Abstract:
:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric probe for sulfenic acid in human serum albumin (HSA-SOH) because of lack of specificity. Thionitrobenzoate (TNB) reacted with HSA exposed to hydrogen peroxide, but not control or thiol-blocked HSA. The reaction was biphasic. The first phase was approximately 20-fold faster than the second phase and first order in HSA-SOH and TNB (105 +/- 11 M-1 s-1, 25 degrees C, pH 7.4), allowing quantitative data on HSA-SOH formation and reactivity to be obtained. Exposure of reduced HSA (0.5 mM) to hydrogen peroxide (4 mM, 37 degrees C, 4 min) yielded 0.18 +/- 0.02 mol of HSA-SOH per mol of HSA. HSA-SH reacted with hydrogen peroxide at 2.7 +/- 0.7 M-1 s-1 (37 degrees C, pH 7.4), while HSA-SOH reacted at 0.4 +/- 0.2 M-1 s-1, yielding sulfinic acid (HSA-SO2H), as detected by mass spectrometry. The rate constants of HSA-SOH with targets of analytical interest such as dimedone and sodium arsenite were determined. HSA-SOH did not react appreciably with the plasma reductants ascorbate or urate, nor with free basic amino acids. In contrast, HSA-SOH reacted rapidly with the plasma thiols cysteine, glutathione, homocysteine, and cysteinylglycine at 21.6 +/- 0.2, 2.9 +/- 0.5, 9.3 +/- 0.9, and 55 +/- 3 M-1 s-1 (25 degrees C, pH 7.4), respectively, supporting a role for HSA-SOH in the formation of mixed disulfides.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Turell L,Botti H,Carballal S,Ferrer-Sueta G,Souza JM,Durán R,Freeman BA,Radi R,Alvarez Bdoi
10.1021/bi701520ysubject
Has Abstractpub_date
2008-01-08 00:00:00pages
358-67issue
1eissn
0006-2960issn
1520-4995journal_volume
47pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800515c
更新日期:2008-08-19 00:00:00
abstract::A specific tertiary hydrogen bond that is present between the side-chain hydroxyl group of Tyr30 and the side-chain N delta 1 atom of His17 in the small, monomeric, single-domain protein, barstar, has been perturbed by site-directed mutagenesis of the sole histidine residue (His17) to a glutamine residue. The effect o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00005a027
更新日期:1995-02-07 00:00:00
abstract::The interaction of the 2'-phosphate-containing nucleotides (NADP+, NADPH, 2'-phosphoadenosine 5'-diphosphoribose, and adenosine 2',5'-bisphosphate) with NADP+ -specific isocitrate dehydrogenase was studied by using 31P NMR spectroscopy. The separate resonances corresponding to free and bound nucleotides, characteristi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00303a015
更新日期:1984-04-10 00:00:00
abstract::Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a024
更新日期:1992-05-26 00:00:00
abstract::Alpha-synuclein plays a key role in the pathogenesis of many neurodegenerative diseases. To date, its cellular role has yet to be determined, although it has been proposed to be connected to calcium and G protein-mediated dopamine signaling. Alpha-synuclein is known to bind strongly to model membrane surfaces where it...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0487140
更新日期:2005-01-18 00:00:00
abstract::There are a small number of peptides derived from proteins that have a propensity to adopt structure in aqueous solution which is similar to the structure they possess in the parent protein. There are far fewer examples of protein fragments which adopt stable nonnative structures in isolation. Understanding how nonnat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990320z
更新日期:1999-06-01 00:00:00
abstract::The smooth muscle basic calponin interacts with F-actin and inhibits the actomyosin ATPase in a calmodulin or phosphorylation modulated manner. It also binds in vitro to microtubules and its acidic isoform, present in nonmuscle cells, and co-localizes with microfilaments and microtubules in cultured neurons. To assess...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020336g
更新日期:2003-02-11 00:00:00
abstract::A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049323o
更新日期:2004-08-17 00:00:00
abstract::Time-resolved infrared (TRIR) techniques have been employed to study the reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome c oxidase (CcO). The ligation dynamics immediately following photodissociation have been investigated using picosecond TRIR spectroscopy and linear dichroism. The r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00168a015
更新日期:1994-01-18 00:00:00
abstract::Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00629a014
更新日期:1977-05-17 00:00:00
abstract::The association of nucleolar phosphoprotein C23 with preribosomal ribonucleoprotein (RNP) particles was examined in Novikoff hepatoma nucleoli. RNA was labeled with [3H]uridine for various times in cell suspensions, and RNP particles were extracted from isolated nucleoli and fractionated by sucrose gradient ultracentr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a063
更新日期:1986-10-07 00:00:00
abstract::A common motif in protein structures is the assembly of alpha-helices. Natural alpha-helical assemblies, such as helical bundles and coiled coils, consist of multiple right-handed alpha-helices. Here we design a protein complex containing both left-handed and right-handed helices, with peptides of D- and L-amino acids...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010725v
更新日期:2001-07-31 00:00:00
abstract::Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a024
更新日期:1977-05-03 00:00:00
abstract::The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00177
更新日期:2018-05-15 00:00:00
abstract::We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00060
更新日期:2015-05-26 00:00:00
abstract::Catalase (hydroperoxidase) HPII of Escherichia coli is the largest catalase so far characterized, existing as a homotetramer of 84 kDa subunits. Each subunit has a core structure that closely resembles small subunit catalases, supplemented with an extended N-terminal sequence and compact flavodoxin-like C-terminal dom...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034208j
更新日期:2003-05-20 00:00:00
abstract::Recent evidence adds support to a traditional concept according to which the eukaryotic nucleus is organized into functional domains by scaffold or matrix attachment regions (S/MARs). These regions have previously been predicted to have a high potential for stress-induced duplex destabilization (SIDD). Here we report ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026496+
更新日期:2003-01-14 00:00:00
abstract::DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex-hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high pero...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9006786
更新日期:2009-08-25 00:00:00
abstract::First identified on the surface of lymphoids as a type II transmembrane protein, CD38 has now been established to have dual functions not only as a receptor but also as a multifunctional enzyme,catalyzing the synthesis of and hydrolysis of a general calcium messenger molecule, cyclic ADP-ribose(cADPR). The receptorial...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:2008-12-30 00:00:00
abstract::T4 polynucleotide kinase is widely used for 5'-phosphorylation of DNA and RNA oligonucleotide termini, but no natural protein enzyme is capable of 3'-phosphorylation. Here, we report the in vitro selection of deoxyribozymes (DNA enzymes) capable of DNA oligonucleotide 3'-phosphorylation, using a 5'-triphosphorylated R...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00151
更新日期:2016-05-10 00:00:00
abstract::Among the 118 genes upregulated by Pseudomonas aeruginosa in response to iron starvation [Ochsner, U. A., Wilderman, P. J., Vasil, A. I., and Vasil, M. L. (2002) Mol. Microbiol. 45, 1277-1287], we focused on the products of the two genes encoding electron transfer proteins, as a means of identifying the redox partners...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7013135
更新日期:2007-10-30 00:00:00
abstract::Piscidin 1 (Pis-1) is a novel cytotoxic peptide with a cationic alpha-helical structure that was isolated from the mast cells of hybrid striped bass [Silphaduang, U., and Noga, E. J. (2001) Nature 414, 268-269]. Pis-1 is not selective for bacterial versus mammalian cells. In the present study, to develop novel antibio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062233u
更新日期:2007-03-27 00:00:00
abstract::The side chains of the interacting pair Asp237(helix VII)-Lys358(helix XI) or Asp240(helix VII)-Lys319(helix X) in the lactose permease of Escherichia coli were extended by replacement with Glu and/or Arg or by site-specific derivatization of single-Cys replacement mutants. Iodoacetic acid was used to carboxymethylate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00089a019
更新日期:1993-09-28 00:00:00
abstract::The interaction of linker histone H1 with both linear and superhelical double-stranded DNA has been investigated at low ionic strengths. Gel mobility retardation experiments demonstrate strikingly different behavior for the two forms of DNA. First, the experiments strongly suggest that linker histone binds to superhel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0497704
更新日期:2004-06-22 00:00:00
abstract::The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00140
更新日期:2015-04-21 00:00:00
abstract::Bleeding, the most serious complication of thrombolytic therapy with tissue-type plasminogen activator (t-PA), is thought to result from lysis of fibrin in hemostatic plugs and from the systemic lytic state caused by unopposed plasmin. One mechanism by which systemic plasmin can impair hemostasis is by partially degra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0525730
更新日期:2006-04-04 00:00:00
abstract::Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972010r
更新日期:1998-03-10 00:00:00
abstract::The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801604x
更新日期:2008-11-18 00:00:00
abstract::A new quantitative technique for measuring the binding of proteins to membranes is described. The method is based on a combination of total internal reflection fluorescence microscopy and the preparation of supported planar bilayers. Specific and reversible binding of a fluorescence-labeled monoclonal antibody to lipi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00458a036
更新日期:1990-02-13 00:00:00
abstract::We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00431a041
更新日期:1989-03-07 00:00:00