当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Analysis and computer simulation of aerobic oxidation of reduced nicotinamide adenine dinucleotide catalyzed by horseradish peroxidase.

Analysis and computer simulation of aerobic oxidation of reduced nicotinamide adenine dinucleotide catalyzed by horseradish peroxidase.

Abstract：

:Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxidase. About 2 mol of oxyperoxidase was formed per mol of H2O2. When a considerable amount of the ferric enzyme still remained, the initial burst was followed by an induction phase. In this phase the rate of oxyperoxidase formation from the ferric enzyme increased with the decrease of the ferric enzyme and an approximately exponential increase of oxyperoxidase was observed. A rapid oxidation of NADH suddenly began at the end of the induction phase and the oxidation continued at a relatively constant rate. In the steady state, oxygen was consumed and H2O2 accumulated. A drastic terminating reaction suddenly set in when the oxygen concentration decreased under a certain level. During the reaction, H2O2 disappeared accompanying an accelerated oxidation of NADH and the enzyme returned to the ferric form after a transient increase of peroxidase compound II. Time courses of NADH oxidation, O2 consumption, H2O2 accumulation, and formation of enzyme intermediates could be simulated with an electronic computer using 11 elementary reactions and 9 rate equations. The results were also discussed in relation to the mechanism for oscillatory responses of the reaction that appeared in an open system with a continuous supply of oxygen.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Yokota K,Yamazaki I

doi

10.1021/bi00628a024

subject

Has Abstract

pub_date

1977-05-03 00:00:00

pages

1913-20

issue

9

eissn

0006-2960

issn

1520-4995

journal_volume

16

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • The 2.15 A crystal structure of Mycobacterium tuberculosis chorismate mutase reveals an unexpected gene duplication and suggests a role in host-pathogen interactions.

    abstract：:Chorismate mutase catalyzes the first committed step toward the biosynthesis of the aromatic amino acids, phenylalanine and tyrosine. While this biosynthetic pathway exists exclusively in the cell cytoplasm, the Mycobacterium tuberculosis enzyme has been shown to be secreted into the extracellular medium. The secretor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0606445

    authors： Qamra R,Prakash P,Aruna B,Hasnain SE,Mande SC

    更新日期：2006-06-13 00:00:00

  • Radical redesign of a tandem array of four R67 dihydrofolate reductase genes yields a functional, folded protein possessing 45 substitutions.

    abstract：:R67 dihydrofolate reductase (DHFR) is a plasmid-encoded, type II enzyme. Four monomers (78 amino acids long) assemble into a homotetramer possessing 222 symmetry. In previous studies, a tandem array of four R67 DHFR gene copies was fused in frame to generate a functional monomer named Quad1. This protein possessed the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1005943

    authors： Feng J,Grubbs J,Dave A,Goswami S,Horner CG,Howell EE

    更新日期：2010-08-31 00:00:00

  • Atomic force microscopy imaging reveals the domain structure of polycystin-1.

    abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300134b

    authors： Oatley P,Stewart AP,Sandford R,Edwardson JM

    更新日期：2012-04-03 00:00:00

  • Structure and mechanism of DNA polymerase β.

    abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500139h

    authors： Beard WA,Wilson SH

    更新日期：2014-05-06 00:00:00

  • Multiple forms of phosphodeoxyribomutase from Escherichia coli. Physical and chemical characterization.

    abstract：:Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00674a021

    authors： Leer JC,Hammer-Jespersen K

    更新日期：1975-02-11 00:00:00

  • Kinetics and thermodynamics of the mechanism of interaction of sodium phytate with alpha-globulin.

    abstract：:The precipitation mechanism of alpha-globulin in the presence of myo-inositol hexaphosphate (sodium phytate) was studied in detail. The maximum interaction was found at pH 2.3 where the protein was in a dissociated state having an 8.3S aggregate and a 1.5S monomer. This interaction was predominantly dependent upon the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00064a035

    authors： Rajendran S,Prakash V

    更新日期：1993-04-06 00:00:00

  • The 56-59-kilodalton protein identified in untransformed steroid receptor complexes is a unique protein that exists in cytosol in a complex with both the 70- and 90-kilodalton heat shock proteins.

    abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00473a021

    authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

    更新日期：1990-05-29 00:00:00

  • Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation.

    abstract：:Site-directed mutagenesis, electron microscopy, and X-ray crystallography were used to probe the structural basis of annexin IV-induced membrane aggregation and the inhibition of this property by protein kinase C phosphorylation. Site-directed mutants that either mimic (Thr6Asp, T6D) or prevent (Thr6Ala, T6A) phosphor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002507s

    authors： Kaetzel MA,Mo YD,Mealy TR,Campos B,Bergsma-Schutter W,Brisson A,Dedman JR,Seaton BA

    更新日期：2001-04-03 00:00:00

  • Crystal structures of two potent nonamidine inhibitors bound to factor Xa.

    abstract：:There has been intense interest in the development of factor Xa inhibitors for the treatment of thrombotic diseases. Our laboratory has developed a series of novel non-amidine inhibitors of factor Xa. This paper presents two crystal structures of compounds from this series bound to factor Xa. The first structure is de...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0264061

    authors： Adler M,Kochanny MJ,Ye B,Rumennik G,Light DR,Biancalana S,Whitlow M

    更新日期：2002-12-31 00:00:00

  • EGF-mCherry Fusion Protein Expressed in E. coli Shows Product Heterogeneity but a High Biological Activity.

    abstract：:The epidermal growth factor receptor (EGFR) is a transmembrane protein involved in cell signaling processes, and dysregulation of its activity often drives tumor growth. EGFR is a clinically validated tumor marker and target for antibodies and tyrosine kinase inhibitors. We demonstrate that a fusion protein of the nat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00021

    authors： Feiner RC,Pennè I,Müller B,Müller KM

    更新日期：2019-02-26 00:00:00

  • Ciliary dynein conformational changes as evidenced by the extrinsic fluorescent probe 8-anilino-1-naphthalenesulfonate.

    abstract：:The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding sites and that experimentally applied ANS concentration...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00347a012

    authors： Saucier AC,Mariotti S,Anderson SA,Purich DL

    更新日期：1985-12-17 00:00:00

  • Electrostatic optimization of the conformational energy landscape in a metamorphic protein.

    abstract：:The equilibrium unfolding reaction of Ltn, a metamorphic C-class chemokine, was monitored by tryptophan fluorescence to determine unfolding free energies. Measurements revealed that addition of 150 mM NaCl stabilized the Ltn chemokine fold by approximately 1 kcal/mol. Specific mutations involving Arg23 and Arg43 also ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300842j

    authors： Tyler RC,Wieting JC,Peterson FC,Volkman BF

    更新日期：2012-11-13 00:00:00

  • Regulation of phosphatidate phosphatase activity from the yeast Saccharomyces cerevisiae by phospholipids.

    abstract：:Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952808f

    authors： Wu WI,Carman GM

    更新日期：1996-03-26 00:00:00

  • Ligand binding properties of bacterial hemoglobins and flavohemoglobins.

    abstract：:Bacterial hemoglobins and flavohemoglobins share a common globin fold but differ otherwise in structural and functional aspects. The bases of these differences were investigated through kinetic studies on oxygen, carbon monoxide, and nitric oxide binding. The novel bacterial hemoglobins from Clostridium perfringens an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047389d

    authors： Farrés J,Rechsteiner MP,Herold S,Frey AD,Kallio PT

    更新日期：2005-03-15 00:00:00

  • Solution conformation of asparagine-linked oligosaccharides: alpha(1-2)-, alpha(1-3)-, beta(1-2)-, and beta(1-4)-linked units.

    abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00284a021

    authors： Brisson JR,Carver JP

    更新日期：1983-07-19 00:00:00

  • Low-temperature solid-state 13C NMR studies of the retinal chromophore in rhodopsin.

    abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00380a018

    authors： Smith SO,Palings I,Copié V,Raleigh DP,Courtin J,Pardoen JA,Lugtenburg J,Mathies RA,Griffin RG

    更新日期：1987-03-24 00:00:00

  • Topological equilibria of ion channel peptides in oriented lipid bilayers revealed by 15N solid-state NMR spectroscopy.

    abstract：:Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050866n

    authors： Sudheendra US,Bechinger B

    更新日期：2005-09-13 00:00:00

  • Using saturation-recovery EPR to measure distances in proteins: applications to photosystem II.

    abstract：:The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00117a033

    authors： Hirsh DJ,Beck WF,Innes JB,Brudvig GW

    更新日期：1992-01-21 00:00:00

  • Arginine-42 and threonine-45 are required for FAD incorporation and catalytic activity in human monoamine oxidase B.

    abstract：:Monoamine oxidase B (MAO B) is an integral protein of the outer mitochondrial membrane that is involved in the deamination of vasoactive and neuroactive amines. The oxidation of these amine substrates requires the cofactor FAD, which is covalently bound to Cys-397 of human MAO B. Previously, Glu-34 and Tyr-44 of MAO B...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9806910

    authors： Kirksey TJ,Kwan SW,Abell CW

    更新日期：1998-09-01 00:00:00

  • Conformational changes and substrate recognition in Pseudomonas aeruginosa D-arginine dehydrogenase.

    abstract：:DADH catalyzes the flavin-dependent oxidative deamination of d-amino acids to the corresponding α-keto acids and ammonia. Here we report the first X-ray crystal structures of DADH at 1.06 Å resolution and its complexes with iminoarginine (DADH(red)/iminoarginine) and iminohistidine (DADH(red)/iminohistidine) at 1.30 Å...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1005865

    authors： Fu G,Yuan H,Li C,Lu CD,Gadda G,Weber IT

    更新日期：2010-10-05 00:00:00

  • Electron spin echo modulation and nuclear relaxation studies of staphylococcal nuclease and its metal-coordinating mutants.

    abstract：:Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00421a010

    authors： Serpersu EH,McCracken J,Peisach J,Mildvan AS

    更新日期：1988-10-18 00:00:00

  • Ruthenium-iron hybrid hemoglobins as a model for partially liganded hemoglobin: oxygen equilibrium curves and resonance Raman spectra.

    abstract：:The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00447a050

    authors： Ishimori K,Tsuneshige A,Imai K,Morishima I

    更新日期：1989-10-17 00:00:00

  • Fourier transforms infrared difference spectroscopy of secondary quinone acceptor photoreduction in proton transfer mutants of Rhodobacter sphaeroides.

    abstract：:In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00045a013

    authors： Nabedryk E,Breton J,Hienerwadel R,Fogel C,Mäntele W,Paddock ML,Okamura MY

    更新日期：1995-11-14 00:00:00

  • Structure of Petunia hybrida defensin 1, a novel plant defensin with five disulfide bonds.

    abstract：:The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by (1)H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose stru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034379o

    authors： Janssen BJ,Schirra HJ,Lay FT,Anderson MA,Craik DJ

    更新日期：2003-07-15 00:00:00

  • Reconsidering the mechanism of polyglutamine peptide aggregation.

    abstract：:There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all of these diseases, the mutant protein aggregates into neuronal inclusions; it is generally, although not universally, believed tha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700806c

    authors： Lee CC,Walters RH,Murphy RM

    更新日期：2007-11-06 00:00:00

  • Catalytic properties of hairpin ribozymes derived from Chicory yellow mottle virus and arabis mosaic virus satellite RNAs.

    abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00048a024

    authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

    更新日期：1995-12-05 00:00:00

  • Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

    abstract：:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035924o

    authors： Tao L,English AM

    更新日期：2004-04-06 00:00:00

  • Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings.

    abstract：:Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050152c

    authors： Jain NU,Tjioe E,Savidor A,Boulie J

    更新日期：2005-06-28 00:00:00

  • Selective inactivation of parvulin-like peptidyl-prolyl cis/trans isomerases by juglone.

    abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973162p

    authors： Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

    更新日期：1998-04-28 00:00:00

  • Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site.

    abstract：:Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00271a036

    authors： Dalbey RE,Wells JA,Yount RG

    更新日期：1983-01-18 00:00:00