Electrostatic optimization of the conformational energy landscape in a metamorphic protein.

abstract：:The highly conserved amino acid sequence PCDGPGRGGTC in both photosystem I reaction center core proteins PsaA and PsaB has been predicted to contribute the four cysteine ligands for coordination of the 4Fe-4S iron-sulfur cluster FX, and we have proposed a working model for the binding of PsaC to this domain of the rea...

journal_title：Biochemistry

authors： Rodday SM,Webber AN,Bingham SE,Biggins J

更新日期：1995-05-16 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...

journal_title：Biochemistry

authors： Wen JD,Gray DM

更新日期：2004-03-09 00:00:00

abstract：:The borrelidin polyketide synthase (PKS) begins with a carboxylated substrate and, unlike typical decarboxylative loading PKSs, retains the carboxy group in the final product. The specificity and tolerance of incorporation of carboxyacyl substrate into type I PKSs have not been explored. Here, we show that the first e...

journal_title：Biochemistry

authors： Hagen A,Poust S,de Rond T,Yuzawa S,Katz L,Adams PD,Petzold CJ,Keasling JD

更新日期：2014-09-30 00:00:00

abstract：:The chemoreceptors of Escherichia coli are homodimeric membrane proteins that cluster in patches near the cell poles. They convert environmental stimuli into intracellular signals that control flagellar rotation. The functional domains of a receptor are physically separated by the cell membrane. Chemoeffectors bind to...

journal_title：Biochemistry

authors： Draheim RR,Bormans AF,Lai RZ,Manson MD

更新日期：2005-02-01 00:00:00

abstract：:Galactose oxidase is a remarkable enzyme containing a metalloradical redox cofactor capable of oxidizing a variety of primary alcohols during enzyme turnover. Recent studies using 1-O-methyl alpha-D-galactopyranoside have revealed an unusually large kinetic isotope effect (KIE) for oxidation of the alpha-deuterated al...

journal_title：Biochemistry

authors： Whittaker MM,Whittaker JW

更新日期：2001-06-19 00:00:00

abstract：:We have designed a novel estrogen-responsive unit, overERE, which consists of two overlapping ERE separated by 5 bp (center-to-center). In gel retardation assays, this sequence forms a low-mobility complex that migrates like an estrogen receptor tetramer. The receptor-overERE complex was specific and was supershifted ...

journal_title：Biochemistry

authors： Massaad C,Coumoul X,Sabbah M,Garlatti M,Redeuilh G,Barouki R

更新日期：1998-04-28 00:00:00

abstract：:In order to more clearly define the structure of human plasma fibronectin (PFn) under physiologic buffer conditions, we determined the mean harmonic rotational relaxation times (rho H) of PFn and the thrombin-derived 190/170-kDa PFn fragment using steady-state fluorescence polarization. These measurements utilized the...

journal_title：Biochemistry

authors： Benecky MJ,Kolvenbach CG,Wine RW,DiOrio JP,Mosesson MW

更新日期：1990-03-27 00:00:00

abstract：:Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...

journal_title：Biochemistry

authors： Lin LF,Houng A,Reed GL

更新日期：2000-04-25 00:00:00

abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...

journal_title：Biochemistry

authors： Burgess AW,Lloyd CJ,Smith S,Stanley E,Walker F,Fabri L,Simpson RJ,Nice EC

更新日期：1988-07-12 00:00:00

abstract：:YtvA is a blue light sensor protein composed of an N-terminal LOV (light-oxygen-voltage) domain, a linker helix, and the C-terminal sulfate transporter and anti-σ factor antagonist domain. YtvA is believed to act as a positive regulator for light and salt stress responses by regulating the σB transcription factor. Alt...

journal_title：Biochemistry

authors： Choi S,Nakasone Y,Hellingwerf KJ,Terazima M

更新日期：2016-06-07 00:00:00

abstract：:The direct electrochemistry of the cytochrome c/cytochrome b5 and cytochrome c/plastocyanin complexes has been investigated at edge-plane graphite and modified gold electrode surfaces, which are selective for one of the two components of the complex. Electrochemical response of one protein at an otherwise electrostati...

journal_title：Biochemistry

authors： Bagby S,Barker PD,Guo LH,Hill HA

更新日期：1990-04-03 00:00:00

abstract：:Tropomyosins from bovine aorta and pulmonary artery exhibit identical electrophoretic patterns in sodium dodecyl sulfate but differ from tropomyosins of either chicken gizzard or rabbit skeletal muscle. Each of the four tropomyosins binds readily to skeletal muscle F-actin as indicated by their sedimentation with acti...

journal_title：Biochemistry

authors： Yamaguchi M,Ver A,Carlos A,Seidel JC

更新日期：1984-02-14 00:00:00

abstract：:The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...

journal_title：Biochemistry

authors： Hayes GR,Williams AM,Lucas JJ,Enns CA

更新日期：1997-04-29 00:00:00

abstract：:The reaction of cytochrome c oxidase with dioxygen has been studied by means of time-resolved measurements of electrical membrane potential (DeltaPsi). Microsecond time resolution was achieved by starting with the CO-inhibited enzyme, which was photolyzed after addition of oxygen. The time course of the reaction could...

journal_title：Biochemistry

authors： Jasaitis A,Verkhovsky MI,Morgan JE,Verkhovskaya ML,Wikström M

更新日期：1999-03-02 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:Static FTIR light-induced difference spectra have been recorded for reaction centers from Rhodopseudomonas viridis in the following charge-separated states: P+QA(-)-PQA, P+QB(-)-PQB, I(-)-I, I-QA(-)-IQA, and I-QA(2-)-IQA. A comparison of the I(-)-I difference spectra with the I-QA(-)-IQA difference spectra reveals new...

journal_title：Biochemistry

authors： Buchanan S,Michel H,Gerwert K

更新日期：1992-02-11 00:00:00

abstract：:Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...

journal_title：Biochemistry

authors： James NG,Berger CL,Byrne SL,Smith VC,MacGillivray RT,Mason AB

更新日期：2007-09-18 00:00:00

abstract：:Because of its ability to selectively glucosylate misfolded glycoproteins, UDP-glucose:glycoprotein glucosyltransferase (UGGT) functions as a folding sensor in the glycoprotein quality control system in the endoplasmic reticulum (ER). The unique property of UGGT derives from its ability to transfer a glucose residue t...

journal_title：Biochemistry

authors： Ohara K,Takeda Y,Daikoku S,Hachisu M,Seko A,Ito Y

更新日期：2015-08-11 00:00:00

abstract：:Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...

journal_title：Biochemistry

authors： Sepuru KM,Rajarathnam K

更新日期：2018-10-16 00:00:00

abstract：:The aspartate receptor is one of the ligand-specific, homodimeric chemoreceptors that detects extracellular attractants and triggers the chemotaxis pathway of Escherichia coli and Salmonella typhimurium. This receptor regulates the activity of the histidine kinase CheA, which forms a kinetically stable complex with th...

journal_title：Biochemistry

authors： Winston SE,Mehan R,Falke JJ

更新日期：2005-09-27 00:00:00

abstract：:Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74 pM). Their molecula...

journal_title：Biochemistry

authors： Yarney TA,Sairam MR,Bhargavi GN,Mohapatra SK

更新日期：1990-04-17 00:00:00

abstract：:Staphylococcus aureus is a leading cause of surgical site infections that results in increased hospital stays due to the development of chronic wounds. Little is known about factors involved in S. aureus' ability to prevent wounds from healing. We discovered a novel secreted protein produced by a surgical site isolate...

journal_title：Biochemistry

authors： Merriman JA,Klingelhutz AJ,Diekema DJ,Leung DY,Schlievert PM

更新日期：2015-08-11 00:00:00

abstract：:A series of human lymphoblastoid cell lines derived from nonleukemic donors are known to be cysteine prototrophs (cys+), while several lymphoblastoid lines derived from leukemic donors are cysteine auxotrophs (cys-). We have tested representative cell lines of each type for their content of cystathionase enzyme activi...

journal_title：Biochemistry

authors： Glode LM,Kriegler MP,Livingston DM

更新日期：1981-03-03 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...

journal_title：Biochemistry

authors： Nagpal A,Valley MP,Fitzpatrick PF,Orville AM

更新日期：2006-01-31 00:00:00

abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...

journal_title：Biochemistry

authors： Allocatelli CT,Cutruzzolà F,Brancaccio A,Brunori M,Qin J,La Mar GN

更新日期：1993-06-15 00:00:00

abstract：:There has been intense interest in the development of factor Xa inhibitors for the treatment of thrombotic diseases. Our laboratory has developed a series of novel non-amidine inhibitors of factor Xa. This paper presents two crystal structures of compounds from this series bound to factor Xa. The first structure is de...

journal_title：Biochemistry

authors： Adler M,Kochanny MJ,Ye B,Rumennik G,Light DR,Biancalana S,Whitlow M

更新日期：2002-12-31 00:00:00

abstract：:Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...

journal_title：Biochemistry

authors： Lee JE,Ciccarelli RB,Jennette KW

更新日期：1982-02-16 00:00:00

abstract：:Reducing sugars such as glucose react nonenzymatically with protein amino groups to initiate a posttranslational modification process known as advanced glycosylation. Nucleotide bases also participate in advanced glycosylation reactions, producing DNA-linked advanced glycosylation endproducts (AGEs) that cause mutatio...

journal_title：Biochemistry

authors： Papoulis A,al-Abed Y,Bucala R

更新日期：1995-01-17 00:00:00