Abstract:
:Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different molecular forms of the enzyme thus isolated were shown not to be products of the column chromatography per se. The amino acid composition as well as the N-terminal amino acid were found to be identical for the different forms. Molecular weight determinations demonstrated that all four forms of the enzyme consist of a single polypeptide chain with a molecular weight of 45,000 plus or minus 1000. Measurements of partial specific volumes, sedimentation coefficients, and absorption coefficients for form I and form II did not reveal any differences. It is concluded that the multiple forms of phosphodeoxyribomutase are caused by modifications of the polypeptide chain. Evidence is presented that form II is formed in vitro from form I by deamidation. It is probable that this deamidation occurs in vivo also. The different forms displayed only minor changes with respect to KM for substrate and cofactor. Greater differences seem to exist among the four enzyme forms with respect to VM and to cobalt binding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Leer JC,Hammer-Jespersen Kdoi
10.1021/bi00674a021subject
Has Abstractpub_date
1975-02-11 00:00:00pages
599-607issue
3eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::R67 dihydrofolate reductase (DHFR) is a type II DHFR produced by bacteria as a resistance mechanism to the increased clinical use of the antibacterial drug trimethoprim. Type II DHFRs are not homologous in either sequence or structure with chromosomal DHFRs. The type II enzymes contain four identical subunits which fo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0026425
更新日期:2001-04-10 00:00:00
abstract::Oxalate is the strongest known inhibitor of yeast cytochrome b2 activity. We have used spectrophotometric titration, temperature-jump relaxation, and calorimetry in an investigation of the interaction between enzyme and inhibitor. The titration data are consistent with noncooperative binding to one site per subunit. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00647a002
更新日期:1976-01-27 00:00:00
abstract::Three spin-labeled derivatives of the voltage-gated peptide alamethicin were prepared with nitroxides at the C-terminal phenyalaninol, and at positions 9 and 15 in the amino acid sequence. In addition, three spin-labeled derivatives of an analog of alamethicin where alpha-methylalanine residues are replaced by leucine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951985d
更新日期:1996-01-16 00:00:00
abstract::It has been shown that in bovine heart submitochondrial particles, antimycin and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) inhibit the oxidation of NADH, succinate, and reduced ubiquinone incompletely, the uninhibited rate being about 20-40 nmol of substrate oxidized min-1 (mg of protein)-1. By contrast, rotenone, cy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00268a045
更新日期:1982-12-07 00:00:00
abstract::β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500074w
更新日期:2014-03-18 00:00:00
abstract::We have labeled the primer binding domain of murine leukemia virus reverse transcriptase (MuLV RT) by covalently cross-linking 5' end labeled d(T)8 to MuLV RT, using ultraviolet light energy. The specificity and the functional significance of the primer cross-linking reaction were demonstrated by the fact that (i) oth...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00240a014
更新日期:1991-07-02 00:00:00
abstract::We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00297a005
更新日期:1984-01-17 00:00:00
abstract::The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00678a002
更新日期:1975-04-08 00:00:00
abstract::The thermotropic behavior and molecular properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-dihydrosterculoyl-sn-glycero-3-phosphoethanolamine (PDSPE) have been investigated by 2H NMR spectroscopy using samples selectively labeled at the 5'-, 9'-, 10'-, and 16'-positions of the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00325a038
更新日期:1985-02-12 00:00:00
abstract::The reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) is accompanied by exceptional values for rate enhancement (k(cat)/k(non) = 7.1 × 10(16)) and catalytic proficiency [(k(cat)/K(M))/k(non) = 4.8 × 10(22) M(-1)]. Although a stabilized vinyl carbanion/carbene intermediate is located on the reactio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2012355
更新日期:2011-10-04 00:00:00
abstract::Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00288a016
更新日期:1983-09-13 00:00:00
abstract::Two compounds have been synthesized that feature a photosensitive o-nitrobenzyl moiety attached directly to the carbamate nitrogen of carbamoylcholine. The well-characterized acetylcholine analogue, carbamoylcholine, was released from these derivatives in response to laser light pulses at wavelengths between 300 and 3...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00355a052
更新日期:1986-04-08 00:00:00
abstract::Protein amyloid fibrils are originally identified as pathological entities in a variety of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Recent studies have revealed that amyloid fibrils also serve as functional protein assemblies to fulfill a wide range of biological functions. Decip...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.9b01069
更新日期:2020-02-11 00:00:00
abstract::There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all of these diseases, the mutant protein aggregates into neuronal inclusions; it is generally, although not universally, believed tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700806c
更新日期:2007-11-06 00:00:00
abstract::The biosynthesis of epothilones, a family of hybrid polyketide (PK)/nonribosomal peptide (NRP) antitumor agents, provides an ideal system to study a hybrid PK/NRP natural product with significant biomedical value. Here the third enzyme involved in epothilone production, the five domain 195 kDa polyketide synthase (PKS...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020006w
更新日期:2002-04-30 00:00:00
abstract::Interaction between aggregates of amyloid beta protein (Abeta) and membranes has been hypothesized by many to be a key event in the mechanism of neurotoxicity associated with Alzheimer's disease (AD). Proposed membrane-related mechanisms of neurotoxicity include ion channel formation, membrane disruption, changes in m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9006397
更新日期:2009-09-22 00:00:00
abstract::Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9004107
更新日期:2009-06-16 00:00:00
abstract::Annexin V is a Ca(2+)-dependent, phospholipid-binding protein that may have one or more membrane-related functions. The binding of annexin V to phospholipids in a detergent micelle matrix was studied to attempt to determine directly the stoichiometry of specific phospholipid-binding sites and the importance of negativ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00094a030
更新日期:1993-11-02 00:00:00
abstract::A cutinase-type polyesterase from Saccharomonospora viridis AHK190 (Cut190) has been shown to degrade the inner block of polyethylene terephthalate. A unique feature of Cut190 is that its function and stability are regulated by Ca2+ binding. Our previous crystal structure analysis of Cut190S226P showed that one Ca2+ b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00624
更新日期:2018-09-11 00:00:00
abstract::Oligomerization of numerous G protein-coupled receptors has been documented, including the prototypic family B secretin receptor. The clinical significance of oligomerization of this receptor became clear with the recent observation that a misspliced form present in pancreatic cancer could associate with the wild-type...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060494y
更新日期:2006-07-11 00:00:00
abstract::Helices IV and V in the lactose permease of Escherichia coli contain the major determinants for substrate binding [Glu126 (helix IV), Arg144 (helix V), and Cys148 (helix V)]. Structural and dynamic features of this region were studied by using site-directed sulfhydryl modification of 48 single-Cys replacement mutants ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010866x
更新日期:2001-09-04 00:00:00
abstract::Sf9 cells infected with a recombinant baculovirus containing the gene for human prorenin were cultured in the presence of [3H]mannose. In vivo labeled prorenin was isolated by immunoprecipitation from the culture medium and digested with Pronase. The oligosaccharide structures on the resulting glycopeptides were analy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00195a022
更新日期:1994-07-26 00:00:00
abstract::Beta2-microglobulin (beta2-m) can form dialysis-related amyloid deposits. The structure of a fragment of beta2-m (K3, Ser20-Lys41) in the oligomeric state has recently been solved. We modeled equilibrium structures of K3 oligomers with different organizations (single and double layers) and morphologies (linear-like an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7019194
更新日期:2008-02-26 00:00:00
abstract::Human telomeric DNA consists of tandem repeats of the DNA sequence d(GGGTTA). Oligodeoxynucleotide telomere models such as d[A(GGGTTA)(3)GGG] (Tel22) fold in a cation-dependent manner into quadruplex structures consisting of stacked G-quartets linked by d(TTA) loops. NMR has shown that in Na(+) solutions Tel22 forms a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901357r
更新日期:2010-01-12 00:00:00
abstract::A PS I membrane preparation from a PS II deficient mutant of Synechocystis sp. PCC 6803 (psb DI/DII/C) was investigated by picosecond photovoltage and fluorescence measurements. The photovoltage kinetics show two distinct phases. At low excitation energies the fast phase correlates with the fluorescence decay time con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00195a001
更新日期:1994-07-26 00:00:00
abstract::N-Methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, is responsible for the removal of a wide variety of alkylated base lesions in DNA, e.g., N-alkylpurines and cyclic ethenoadducts of adenine, guanine, and cytosine. These lesions, some of which are mutagenic and toxic, are generated endogenously or b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972313l
更新日期:1998-01-13 00:00:00
abstract::The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00132a013
更新日期:1992-05-05 00:00:00
abstract::The structures of four isomorphous crystals of ternary complexes of chicken heart citrate synthase with D- or L-malate and acetyl coenzyme A or carboxymethyl coenzyme A have been determined by X-ray crystallography and fully refined at 1.9-A resolution. The structures show that both L-malate and D-malate bind in a ver...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00238a028
更新日期:1991-06-18 00:00:00
abstract::The sequence of two overlapping cDNA clones for the zinc metalloproteinase hemorrhagic toxin e (also known as atrolysin e, EC 3.4.24.44) from the venom gland of Crotalus atrox, the Western diamondback rattlesnake, is presented. The assembled cDNA sequence is 1975 nucleotides in length and encodes an open reading frame...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00142a005
更新日期:1992-07-14 00:00:00
abstract::Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00096
更新日期:2020-04-07 00:00:00