Abstract：
:The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by (1)H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose structure has been determined. PhD1 has the fold of the cysteine-stabilized alphabeta motif, consisting of an alpha-helix and a triple-stranded antiparallel beta-sheet, except that it contains a fifth disulfide bond from the first loop to the alpha-helix. The additional disulfide bond is accommodated in PhD1 without any alteration of its tertiary structure with respect to other plant defensins. Comparison of its structure with those of classic, four-disulfide defensins has allowed us to identify a previously unrecognized hydrogen bond network that is integral to structure stabilization in the family.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Janssen BJ,Schirra HJ,Lay FT,Anderson MA,Craik DJdoi
10.1021/bi034379osubject
Has Abstractpub_date
2003-07-15 00:00:00pages
8214-22issue
27eissn
0006-2960issn
1520-4995journal_volume
42pub_type
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