Abstract:
:The effects of two oppositely charged pyrene derivatives, 1-pyrenebutyrylcholine (PBC) and 1-pyrenebutyric acid (PBA), on red blood cell shape have been examined. Both compounds convert normal biconcave erythrocytes into echinocytes. However, with extended incubation time at elevated temperature, the morphology of PBC-induced echinocytes is reversed. Examination of probe uptake confirmed that, in contrast to PBA, equilibration of PBC with intact cells occurs very slowly. For PBA-induced echinocytes, it was possible to quantitate the fraction of probe bound in each half of the bilayer from nanosecond fluorescence measurements. Analysis of the heterogeneous decay showed that 71% of the bound PBA was associated with a lifetime (tau) of 102 ns and 29% with tau = 8 ns. It is likely that the later, highly quenched, component corresponds to fluorophores bound at the cytoplasmic surface because of efficient energy transfer to hemoglobin and that the long component corresponds to probe bound exclusively at the outer surface. Evidence in support of this interpretation was obtained by showing that when the paramagnetic cation Mn2+ bound at the extracellular surface the 102-ns component is quenched. The excimer fluorescence of PBC bound to red cells was examined and found to show time and temperature dependencies which correlate with morphological effects. These results indicate that red cells become crenated with PBC molecules are highly concentrated in the outer bilayer half and that shape reversal is subsequently brought about as PBC permeates and accumulates in the inner bilayer half. Finally, hemolysis protection due to PBC or PBA binding was observed also to show striking correlations with cell shape, In summary, these findings support the hypothesis [Sheetz, M. P., & Singer, S. J. (1974) Proc. Natl. Acad. Sci. U.S.A. 71, 4457] that shape changes are induced in red cells by amphiphilic molecules as a consequence of their relative partitioning between the two halves of the bilayer.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Matayoshi EDdoi
10.1021/bi00556a002subject
Has Abstractpub_date
1980-07-22 00:00:00pages
3414-22issue
15eissn
0006-2960issn
1520-4995journal_volume
19pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Infrared (vibrational) circular dichroism (VCD) has been observed for the DNA models d(CG)5, poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dA-dT).poly(dA-dT), and poly(dA).poly(dT) in the B-conformation in buffered, aqueous solution. The observed results are quantitatively interpreted in terms of the exciton model ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00484a018
更新日期:1990-08-14 00:00:00
abstract::Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0005456
更新日期:2000-08-01 00:00:00
abstract::[15N]Glycine was biosynthetically incorporated into normal cellular N-ras p21 and a position 12 transforming mutant, in order to produce p21 proteins containing several site-specific NMR probes at or near activating positions in the guanine nucleotide binding domain. We have previously assigned all five glycine resona...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00450a035
更新日期:1989-11-28 00:00:00
abstract::Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0267588
更新日期:2003-07-15 00:00:00
abstract::We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00431a041
更新日期:1989-03-07 00:00:00
abstract::A method is described for measuring equilibrium constants of DNA-protein interactions using gel chromatography. This technique has been used to study the sequence-specific interaction of the HinfI restriction endonuclease with DNA. HinfI has a monomeric molecular weight of 31000 and exists as a dimer in its active for...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00333a037
更新日期:1985-06-04 00:00:00
abstract::Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001867c
更新日期:2001-01-09 00:00:00
abstract::MRP1 (or ABCC1) is an ABC membrane protein that transports a wide range of natural products as well as glutathione (GSH)-, glucuronate-, and sulfate-conjugated metabolites. In addition, free GSH is required for MRP1 to transport several chemotherapeutic drugs. However, the mechanisms regulating the influence of GSH on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0268807
更新日期:2003-03-25 00:00:00
abstract::One of the most intriguing questions confronting the bone morphogenetic protein family is the mechanism of ligand recognition, because there are more ligands than receptors. Crystal structures of two type II receptors, ActR-II and BMPR-II, are essentially identical, and a loop structure (A-loop) has been suggested to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3003023
更新日期:2012-09-04 00:00:00
abstract::Product distributions for the reaction of glycolaldehyde labeled with carbon-13 at the carbonyl carbon ([1-(13)C]-GA) catalyzed by triosephosphate isomerase (TIM) in D(2)O at pD 7.0 in the presence of phosphite dianion and in its absence were determined by (1)H NMR spectroscopy. We observe three products for the relat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900636c
更新日期:2009-06-23 00:00:00
abstract::Changes in pH can alter the structure and activity of proteins and may be used by the cell to control molecular function. This coupling can also be used in non-native applications through the design of pH-sensitive biomolecules. For example, the pH (low) insertion peptide (pHLIP) can spontaneously insert into a lipid ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00380
更新日期:2016-08-09 00:00:00
abstract::TPPP/p25 is a brain-specific protein, which induces tubulin polymerization and microtubule (MT) bundling and is enriched in Lewy bodies characteristic of Parkinson's disease [Tirián et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13976-13981]. We identified two human gene sequences, CG1-38 and p25beta, which encoded ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061305e
更新日期:2006-11-21 00:00:00
abstract::ABCG2 is a half-ATP binding cassette (ABC) drug transporter that consists of a nucleotide binding domain (NBD) followed by a transmembrane domain. This half-ABC transporter is thought to form a homodimer in the plasma membrane where it transports anticancer drugs across the biological membranes in an ATP-dependent man...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900675v
更新日期:2009-09-29 00:00:00
abstract::Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00546a032
更新日期:1980-03-04 00:00:00
abstract::The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952052k
更新日期:1996-01-09 00:00:00
abstract::Low pH-induced membrane insertion by diphtheria toxin T domain is crucial for A chain translocation into the cytoplasm. To define the membrane topography of the T domain, the exposure of biotinylated Cys residues to the cis and trans bilayer surfaces was examined using model membrane vesicles containing a deeply inser...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049354j
更新日期:2004-07-20 00:00:00
abstract::The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026099z
更新日期:2002-10-01 00:00:00
abstract::The stability and folding kinetics of wild-type and a mutant staphylococcal nuclease (SNase) at neutral pH are significantly perturbed by the presence of moderate to high concentrations of salts. Very substantial increases in stability toward thermal and urea denaturation were observed; for example, 0.4 M sodium sulfa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000861k
更新日期:2001-02-20 00:00:00
abstract::Serum paraoxonase hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. High serum paraoxonase levels appear to protect against the neurotoxic effects of organophosphorus substrates of this enzyme [Costa et al. (1990) Toxicol. Appl. Pharmacol. 103, 66-76]. The amino acid sequence accounting f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00106a010
更新日期:1991-10-22 00:00:00
abstract::Electrostatic interactions play important roles in the catalysis of chorismate to prephenate by chorismate mutase. Mutation of Gln88 to glutamate in the monofunctional chorismate mutase from Escherichia coli results in an enzyme with a pH profile of activity significantly different from that of the wild type protein. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980217u
更新日期:1998-06-23 00:00:00
abstract::MAP kinase kinase (MAPKK) is a dual specificity protein kinase that phosphorylates and activates MAP kinase in vivo. In this study, four mouse monoclonal single-chain Fv (scFv) antibodies (Y1-6, Y1-7, Y3-6, and Y3-11) that can specifically bind to Xenopus MAPKK were isolated from combinatorial scFv-displaying phage li...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960956f
更新日期:1996-10-08 00:00:00
abstract::The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962884y
更新日期:1997-04-29 00:00:00
abstract::The high-frequency resonance Raman spectra of FeIII yeast native cytochrome c peroxidase (CCP) and five of its mutants [CCP(MI), Phe-51, Leu-48, Lys-48, Asn-235, and Phe-191] were recorded in phosphate buffer, pH 7.0, and in glycerol/phosphate mixtures at 295 and 10 K. Glycerol induces heme coordination changes in som...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00438a024
更新日期:1989-06-13 00:00:00
abstract::Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970380+
更新日期:1997-07-01 00:00:00
abstract::Mass spectrometry was used to identify the sites of covalent attachment of [(14)C]-17alpha-bromoacetamidopropylestradiol ([(14)C]17BAPE(2), an estradiol agonist) to the ligand-binding domain (LBD) of mouse estrogen receptor alpha (ERalpha). A glutathione S-transferase (GST)-LBD chimera protein was overexpressed in Esc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0205092
更新日期:2002-12-31 00:00:00
abstract::The EcoRV restriction endonuclease cleaves DNA at its recognition sequence more readily with Mg2+ as the cofactor than with Mn2+ but, at noncognate sequences that differ from the EcoRV site by one base pair, Mn2+ gives higher rates than Mg2+. A mutant of EcoRV, in which an isoleucine near the active site was replaced ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9523926
更新日期:1996-02-13 00:00:00
abstract::Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a022
更新日期:1987-06-30 00:00:00
abstract::We have studied the hydrogen-exchange kinetics of hemoglobin A0 as a function of ligand, CO, saturation. In the noncooperative system, azide binding to methemoglobin, the alterations in exchange kinetics are proportional to the average degree of ligation. However, in the case of CO binding to deoxyhemoglobin the chang...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00297a015
更新日期:1984-01-17 00:00:00
abstract::Antithrombin III (ATIII) is a plasma-borne serine protease inhibitor that apparently forms covalent complexes with thrombin. The interaction between ATIII and thrombin is enhanced several thousandfold by the glycosaminoglycan, heparin. We have previously proposed that the heparin binding site of ATIII resides within a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00490a010
更新日期:1990-09-25 00:00:00
abstract::A generalized set of fluorescence polarization intensities and electron paramagnetic resonance (EPR) spectra from multiple fluorescent and EPR probes of a specific site on an oriented and immobilized protein element in a biological assembly, combined with anisotropic rotational relaxation studies of the purified and l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00184a004
更新日期:1994-05-10 00:00:00