Abstract:
:TPPP/p25 is a brain-specific protein, which induces tubulin polymerization and microtubule (MT) bundling and is enriched in Lewy bodies characteristic of Parkinson's disease [Tirián et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13976-13981]. We identified two human gene sequences, CG1-38 and p25beta, which encoded homologous proteins, that we termed p20 and p18, respectively. These homologous proteins display 60% identity with tubulin polymerization promoting protein/p25 (TPPP/p25); however, the N-terminal segment of TPPP/p25 is missing. They could be clustered into three subfamilies present in mammals and other vertebrates. We cloned, isolated, and characterized the structural and functional properties of the recombinant human proteins at molecular, ultrastructural, and cellular levels using a number of tools. These data revealed that, while p20 behaved as a disorganized protein similarly to TPPP/p25, which was described as a flexible and inherently dynamic protein with a long unstructured N-terminal tail, p18 was featured in more ordered fashion. TPPP/p25 and p20 specifically attached to MTs causing MT bundling both in vitro and in vivo; p18 protein did not cross-link MTs, and it distributed homogeneously within the cytosol of the transfected HeLa cells. These data indicate that the two shorter homologues display distinct structural features that determine their associations to MTs. The properties of p20 resemble TPPP/p25. The bundling activity of these two proteins results in the stabilization of the microtubular network, which is likely related to their physiological functions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Vincze O,Tökési N,Oláh J,Hlavanda E,Zotter A,Horváth I,Lehotzky A,Tirián L,Medzihradszky KF,Kovács J,Orosz F,Ovádi Jdoi
10.1021/bi061305esubject
Has Abstractpub_date
2006-11-21 00:00:00pages
13818-26issue
46eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Upon blue light irradiation, photoactive yellow protein (PYP) undergoes a conformational change that involves large movements at the N-terminus of the protein. We reasoned that this conformational change might be used to control other protein or peptide sequences if these were introduced as linkers connecting the N- a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400018h
更新日期:2013-05-14 00:00:00
abstract::Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01174
更新日期:2017-08-08 00:00:00
abstract::Two short amino acid motifs, WXGDXNXR and PXWCDRXL, define a large family of inositol polyphosphate 5-phosphatases. We tested the importance of seven of these conserved amino acids to substrate binding and catalysis by mutating each to alanine in the platelet 75 kDa inositol polyphosphate 5-phosphatase II (5-phosphata...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9602627
更新日期:1996-06-18 00:00:00
abstract::The iron K-edge X-ray absorption spectrum of Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) nitrile hydratase in frozen solutions at pH 7 and 9 has been analyzed to determine details of the iron coordination. EXAFS analysis implies two or three sulfur ligands per iron and overall six coordination; together wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960164l
更新日期:1996-08-06 00:00:00
abstract::The RcnR metalloregulator represses the transcription of the Co(II) and Ni(II) exporter, RcnAB. Previous studies have shown that Co(II) and Ni(II) bind to RcnR in six-coordinate sites, resulting in derepression. Here, the roles of His60, His64, and His67 in specific metal recognition are examined. His60 and His64 corr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300886q
更新日期:2013-01-08 00:00:00
abstract::The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtai...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00580a030
更新日期:1979-06-26 00:00:00
abstract::Heme oxygenase (HO) catalyzes the regiospecific cleavage of the porphyrin ring of heme using reducing equivalents and O2 to produce biliverdin, iron, and CO. Because CO has a cytoprotective effect through the p38-MAPK pathway, HO is a potential therapeutic target in cancer. In fact, inhibition of the HO isoform HO-1 r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062264p
更新日期:2007-02-20 00:00:00
abstract::Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The corresp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00504a600
更新日期:1981-01-06 00:00:00
abstract::The three lipoyl (E2plip) domains of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase (PDH) complex of Escherichia coli house the lipoyl-lysine side chain essential for active-site coupling and substrate channelling within the complex. The structure of the unlipoylated form of the innermost ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992978i
更新日期:2000-07-25 00:00:00
abstract::The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00252a016
更新日期:1994-12-06 00:00:00
abstract::Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027006g
更新日期:2003-04-22 00:00:00
abstract::In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035319l
更新日期:2004-01-13 00:00:00
abstract::The regulation of the rate-controlling enzyme in cholesterol biosynthesis and of the incorporation of [14C]oleate into cholesterol esters were studied in established lymphoid cell lines from normal subjects and compared with that of eight patients with genetic abnormalities of lipid metabolism. The activity of 3-hydro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00648a011
更新日期:1976-02-10 00:00:00
abstract::It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00167a038
更新日期:1994-01-11 00:00:00
abstract::Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001272h
更新日期:2000-10-03 00:00:00
abstract::Apo horse spleen ferritin (apo HoSF) was reconstituted to various core sizes (100-3500 Fe3+/HoSF) by depositing Fe(OH)3 within the hollow HoSF interior by air oxidation of Fe2+. Fe2+ and phosphate (Pi) were then added anaerobically at a 1:4 ratio, and both Fe2+ and Pi were incorporated into the HoSF cores. The resulti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982727u
更新日期:1999-05-18 00:00:00
abstract::The reaction of ferric cytochrome c peroxidase (CcP) from Saccharomyces cerevisiae with peroxide produces compound I, characterized by both an oxyferryl iron center and a protein-based free radical. The electron paramagnetic resonance (EPR) signal of the CcP compound I radical can be resolved into a broad majority com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a036
更新日期:1991-02-19 00:00:00
abstract::The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9719814
更新日期:1998-02-10 00:00:00
abstract::The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00515a024
更新日期:1981-06-09 00:00:00
abstract::Conformational states of the Na,K-ATPase and rates of transition between these are studied using the fluorescent dye eosin as a marker for the Na+ form (E1) and occlusion of 86Rb+ as a marker for the K+ form (E2). The aim of the present paper is to propose that the E1 form of the Na,K-ATPase can be liganded with a num...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00194a022
更新日期:1994-07-19 00:00:00
abstract::There is a significant need for new therapeutics to treat infections caused by the biodefense agent Bacillus anthracis. In pursuit of drug discovery against this organism, we have developed novel propargyl-linked inhibitors that target the essential enzyme dihydrofolate reductase (DHFR) from B. anthracis. Previously, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802319w
更新日期:2009-05-19 00:00:00
abstract::The flavocytochrome p-cresol methylhydroxylase from Pseudomonas putida has been reported to have a Mr of 114,000 and to consist of two subunits, a flavoprotein and a cytochrome c, each with a Mr of 58,000. Recent X-ray crystallographic data from our laboratories [Shamala, N., Lim, L. W., Mathews, F. S., McIntire, W., ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a021
更新日期:1986-10-07 00:00:00
abstract::Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200049c
更新日期:2011-04-05 00:00:00
abstract::We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00371a025
更新日期:1986-11-18 00:00:00
abstract::The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00129a010
更新日期:1992-04-14 00:00:00
abstract::The gamma subunits of the 7S nerve growth factor complex (7S NGF) display arginine esteropeptidase activity. By varying the conditions of electrophoresis in acrylamide gel, it has been demonstrated that the gamma-subunit fraction of 7S NGF contains five different proteins, in contrast to the three (gamma1, gamma2, and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00652a016
更新日期:1976-04-06 00:00:00
abstract::D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201378c
更新日期:2011-11-22 00:00:00
abstract::Rat liver sinusoidal endothelial cells (LECs) mediate the removal of hyaluronan (HA) from the circulation via a specific Ca(2+)-independent endocytic receptor. To characterize the receptor biochemically, detergent-soluble extracts were prepared from crude LEC membranes. Using a dot blot assay to quantitate 125I-HA bin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00117a039
更新日期:1992-01-21 00:00:00
abstract::An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026191v
更新日期:2002-10-15 00:00:00
abstract::Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00121a039
更新日期:1992-02-18 00:00:00