当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy.

Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy.

Abstract：

:Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The correspondence between the ionization behavior as determined by proton NMR and fluorometric titrations indicated that fluorescence titrations reflect the ionization behavior of His-159 in both the active enzyme and the methylthio derivative. The ionic strength dependence of the pK was analyzed in terms of simple electrostatic theory and was shown to be consistent with the charge on the protein. The temperature dependence of the pK values of His-159 indicated an increase in the heat of ionization from about 0 to 8 kcal/mol upon removal of the methylthio blocking group from Cys-25. Measurements of the effect of solvent on the pK's and heats of ionization of simple model compounds indicated that the observed shift in enthalpy of ionization of His-159 upon removal of th methylthio group from Cys-25 is not unreasonable in light of the accompanying perturbation is more than 4 pK units in the pK of His-159. The perturbations in enthalpies and free energies are attributed to formation of an ion pair. The ionization behavior of His-159 in thiol-blocked derivatives of papain is consistent with the involvement of His-159 in the deacylation step in papain catalysis.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Johnson FA,Lewis SD,Shafer JA

doi

10.1021/bi00504a600

subject

Has Abstract

pub_date

1981-01-06 00:00:00

pages

52-8

issue

1

eissn

0006-2960

issn

1520-4995

journal_volume

20

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Phase-resolved spectral measurements with several two tryptophan containing proteins.

    abstract：:We have used frequency domain fluorescence techniques to resolve the component emission spectra for several two tryptophan containing proteins (e.g., horse liver alcohol dehydrogenase, sperm whale apomyoglobin, yeast 3-phosphoglycerate kinase, apoazurin from Alcaligenes denitricans). We have first performed multifrequ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00399a047

    authors： Eftink MR,Wasylewski Z,Ghiron CA

    更新日期：1987-12-15 00:00:00

  • Involvement of tryptophan residues at the coenzyme A binding site of carbon monoxide dehydrogenase from Clostridium thermoaceticum.

    abstract：:Carbon monoxide dehydrogenase (CODH) from Clostridium thermoaceticum plays a central role in the newly discovered acetyl-CoA pathway [Wood, H.G., Ragsdale, S.W., & Pezacka, E. (1986) FEMS Microbiol. Rev. 39, 345-362]. The enzyme catalyzes the formation of acetyl-CoA from methyl, carbonyl, and CoA groups, and it has sp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00417a045

    authors： Shanmugasundaram T,Kumar GK,Wood HG

    更新日期：1988-08-23 00:00:00

  • Regioselectivity in the homolytic cleavage of S-adenosylmethionine.

    abstract：:The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101509u

    authors： Kampmeier JA

    更新日期：2010-12-28 00:00:00

  • Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation.

    abstract：:Site-directed mutagenesis, electron microscopy, and X-ray crystallography were used to probe the structural basis of annexin IV-induced membrane aggregation and the inhibition of this property by protein kinase C phosphorylation. Site-directed mutants that either mimic (Thr6Asp, T6D) or prevent (Thr6Ala, T6A) phosphor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002507s

    authors： Kaetzel MA,Mo YD,Mealy TR,Campos B,Bergsma-Schutter W,Brisson A,Dedman JR,Seaton BA

    更新日期：2001-04-03 00:00:00

  • Kinetics of binding of oligosaccharides to a homogeneous pneumococcal antibody: dependence on antigen chain length suggests a labile intermediate complex.

    abstract：:Temperature-jump experiments were performed with di-, tetra-, and hexasaccharides derived from type III pneumococcal polysaccharide using a homogeneous corresponding antibody IgG 45-394. A decrease in stability of the oligosaccharide-antibody complexes with decreasing chain length was observed and entirely reflected i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00637a023

    authors： Maeda H,Schmidt-Kessen A,Engel J,Jaton JC

    更新日期：1977-09-06 00:00:00

  • 31P NMR studies of nucleotide storage in the dense granules of pig platelets.

    abstract：:Detailed 31P NMR measurements have been conducted on pig platelet dense granules and aqueous mixtures of ATP, ADP, MgCl2, and 5HT. The resonance line widths of the dense granule nucleotides were temperature independent above approximately 30 degrees C; below this temperature they exhibit a strong temperature dependenc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00298a004

    authors： Uğurbil K,Fukami MH,Holmsen H

    更新日期：1984-01-31 00:00:00

  • Isolation of peptides from the carboxyl carrier subunit of transcarboxylase. Role of the non-biotinyl peptide in assembly.

    abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00679a010

    authors： Ahmad F,Jacobson B,Chuang M,Brattin W,Wood HG

    更新日期：1975-04-22 00:00:00

  • A spectroscopic study of the temperature induced modifications on ferredoxin folding and iron-sulfur moieties.

    abstract：:Thermal perturbation of the dicluster ferredoxin from Acidianus ambivalens was investigated employing a toolbox of spectroscopic methods. FTIR and visible CD were used for assessing changes of the secondary structure and coarse alterations of the [3Fe4S] and [4Fe4S] cluster moieties, respectively. Fine details of the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700967g

    authors： Todorovic S,Leal SS,Salgueiro CA,Zebger I,Hildebrandt P,Murgida DH,Gomes CM

    更新日期：2007-09-18 00:00:00

  • Interaction between the Rev1 C-Terminal Domain and the PolD3 Subunit of Polζ Suggests a Mechanism of Polymerase Exchange upon Rev1/Polζ-Dependent Translesion Synthesis.

    abstract：:Translesion synthesis (TLS) is a mutagenic branch of cellular DNA damage tolerance that enables bypass replication over DNA lesions carried out by specialized low-fidelity DNA polymerases. The replicative bypass of most types of DNA damage is performed in a two-step process of Rev1/Polζ-dependent TLS. In the first ste...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01282

    authors： Pustovalova Y,Magalhães MT,D'Souza S,Rizzo AA,Korza G,Walker GC,Korzhnev DM

    更新日期：2016-04-05 00:00:00

  • Conformational heterogeneity at position U37 of an all-RNA hairpin ribozyme with implications for metal binding and the catalytic structure of the S-turn.

    abstract：:The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051550i

    authors： Alam S,Grum-Tokars V,Krucinska J,Kundracik ML,Wedekind JE

    更新日期：2005-11-08 00:00:00

  • Mapping the microtubule binding regions of calponin.

    abstract：:The smooth muscle basic calponin interacts with F-actin and inhibits the actomyosin ATPase in a calmodulin or phosphorylation modulated manner. It also binds in vitro to microtubules and its acidic isoform, present in nonmuscle cells, and co-localizes with microfilaments and microtubules in cultured neurons. To assess...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020336g

    authors： Fattoum A,Roustan C,Smyczynski C,Der Terrossian E,Kassab R

    更新日期：2003-02-11 00:00:00

  • Biochemical and functional characterization of recombinant Rhodnius prolixus platelet aggregation inhibitor 1 as a novel lipocalin with high affinity for adenosine diphosphate and other adenine nucleotides.

    abstract：:The Rhodnius prolixus aggregation inhibitor 1 (RPAI-1) is a novel blood-sucking salivary molecule that binds to ADP and attenuates platelet aggregation. In this report, we determine the binding constants and specificity of RPAI-1 for adenine nucleotides and its functional significance. By the Hummel-Dreyer method of e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011015s

    authors： Francischetti IM,Andersen JF,Ribeiro JM

    更新日期：2002-03-19 00:00:00

  • A probe for the mutagenic activity of the carcinogen 4-aminobiphenyl: synthesis and characterization of an M13mp10 genome containing the major carcinogen-DNA adduct at a unique site.

    abstract：:The duplex genome of Escherichia coli virus M13mp10 was modified at a unique site to contain N-(deoxyguanosin-8-yl)-4-aminobiphenyl (dG8-ABP), the major carcinogen-DNA adduct of the human bladder carcinogen 4-aminobiphenyl. A tetradeoxynucleotide containing a single dG8-ABP residue was synthesized by reacting 5'-d(TpG...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00385a019

    authors： Lasko DD,Basu AK,Kadlubar FF,Evans FE,Lay JO Jr,Essigmann JM

    更新日期：1987-06-02 00:00:00

  • Sodium-dependent steps in the redox reactions of the Na+-motive NADH:quinone oxidoreductase from Vibrio harveyi.

    abstract：:The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002545b

    authors： Bogachev AV,Bertsova YV,Barquera B,Verkhovsky MI

    更新日期：2001-06-19 00:00:00

  • Molecular basis of the effects of chloride ion on the acid-base catalyst in the mechanism of pancreatic alpha-amylase.

    abstract：:Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048201t

    authors： Qian M,Ajandouz el H,Payan F,Nahoum V

    更新日期：2005-03-08 00:00:00

  • Normal modes of prion proteins: from native to infectious particle.

    abstract：:Prion proteins (PrP) are the infectious agent in transmissible spongiform encephalopathies (i.e., mad cow disease). To be infectious, prion proteins must undergo a conformational change involving a decrease in α-helical content along with an increase in β-strand content. This conformational change was evaluated by mea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1010514

    authors： Samson AO,Levitt M

    更新日期：2011-03-29 00:00:00

  • Oxidation of the Cyanobactin Precursor Peptide Is Independent of the Leader Peptide and Operates in a Defined Order.

    abstract：:The five-membered nitrogen plus heteroatom rings known as azolines or in their oxidized form as azoles are very common in natural products and drugs. The oxidation of thiazoline to thiazole in the cyanobactin class of natural products is one of the several important transformations that are known to alter the biologic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00835

    authors： Gao S,Ge Y,Bent AF,Schwarz-Linek U,Naismith JH

    更新日期：2018-10-16 00:00:00

  • Calorimetric studies of ligands binding to glutathione S-transferase from the malarial parasite Plasmodium falciparum.

    abstract：:Glutathione S-transferase, from the malarial parasite Plasmodium falciparum (PfGST), exerts a protective role in the organism and is thus considered an interesting target for antimalarial drug development. In contrast to other GSTs, it is present in solution as a tetramer and a dimer in equilibrium, which is induced b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400007g

    authors： Quesada-Soriano I,Barón C,García-Maroto F,Aguilera AM,García-Fuentes L

    更新日期：2013-03-19 00:00:00

  • Studies on the cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA.

    abstract：:The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00696a023

    authors： Ruyechan WT,Wetmur JG

    更新日期：1975-12-16 00:00:00

  • Self-splicing of the group I intron from Anabaena pre-tRNA: requirement for base-pairing of the exons in the anticodon stem.

    abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00082a016

    authors： Zaug AJ,McEvoy MM,Cech TR

    更新日期：1993-08-10 00:00:00

  • The arginine finger loop of yeast and human GAP is a determinant for the specificity toward Ras GTPase.

    abstract：:In this work, we have studied the role of the arginine finger region in determining the specificity of the GTPase activating proteins (GAPs) Saccharomyces cerevisiae Ira2p and human p120-GAP toward yeast Ras2p and human Ha-Ras p21. It is known that p120-GAP can enhance both Ras2p and Ha-Ras GTPase activities, whereas ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010027a

    authors： te Biesebeke R,Krab IM,Parmeggiani A

    更新日期：2001-06-26 00:00:00

  • A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.

    abstract：:Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200644c

    authors： Pak ML,Lakowski TM,Thomas D,Vhuiyan MI,Hüsecken K,Frankel A

    更新日期：2011-09-27 00:00:00

  • Thrombin inactivates acidic fibroblast growth factor but not basic fibroblast growth factor.

    abstract：:Incubation of bovine brain derived acidic fibroblast growth factor (aFGF) with bovine or human thrombin, 0.5 NIH unit/mL, for 24 h at 37 degrees C results in cleavage of the mitogen, generating a 14-kilodalton fragment which has significantly reduced affinity for immobilized heparin as compared to aFGF, and is at leas...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00407a045

    authors： Lobb RR

    更新日期：1988-04-05 00:00:00

  • Structural and guanosine triphosphate/diphosphate requirements for transit peptide recognition by the cytosolic domain of the chloroplast outer envelope receptor, Toc34.

    abstract：:Toc34 is a transmembrane protein located in the outer envelope membrane of chloroplasts and involved in transit peptide recognition. The cytosolic region of Toc34 reveals 34% alpha-helical and 26% beta-strand structure and is stabilized by intramolecular electrostatic interaction. Toc34 binds both chloroplast preprote...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011361+

    authors： Schleiff E,Soll J,Sveshnikova N,Tien R,Wright S,Dabney-Smith C,Subramanian C,Bruce BD

    更新日期：2002-02-12 00:00:00

  • Biochemical and biological characterization of neutrophil chemotactic protein, a novel rabbit CXC chemokine from alveolar macrophages.

    abstract：:The role of interleukin-8 (IL-8) and related CXC chemokines has been demonstrated in many human diseases. However, more profound studies, e.g., by blocking the effect of these inflammatory mediators, request animal models and hence the identification of all human counterparts for commonly used laboratory animals. In t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0011227

    authors： Wuyts A,Schutyser E,Menten P,Struyf S,D'Haese A,Bult H,Opdenakker G,Proost P,Van Damme J

    更新日期：2000-11-28 00:00:00

  • Folding domains as functional tools in allosteric systems: a heme-dependent domain in hemoglobin beta subunits.

    abstract：:We have investigated the denaturation by guanidine hydrochloride (Gdn X HCl), temperature, and pH of hemoglobin beta subunits and of the peptides beta (1-146), beta (56-146), and beta (1-55). The last peptide was insensitive to all of the three agents. In the other polypeptides denaturation by Gdn X HCl and temperatur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00267a024

    authors： Franchi D,Fronticelli C,Bucci E

    更新日期：1982-11-23 00:00:00

  • Inhibition of sodium-calcium exchange in cardiac sarcolemmal membrane vesicles. 1. Mechanism of inhibition by amiloride analogues.

    abstract：:The mechanism by which terminal guanidino nitrogen substituted analogues of amiloride inhibit Na-Ca exchange in purified cardiac sarcolemmal membrane vesicles has been investigated. These inhibitors block both Nai-dependent Ca2+ uptake and Nao-dependent Ca2+ efflux. Inhibition of Na-Ca exchange monitored in K+ is nonc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00407a023

    authors： Slaughter RS,Garcia ML,Cragoe EJ Jr,Reeves JP,Kaczorowski GJ

    更新日期：1988-04-05 00:00:00

  • Exploration of the Effects of γ-Phosphate-Modified ATP Analogues on Histidine Kinase Autophosphorylation.

    abstract：:While two-component systems (TCSs), composed of a sensor histidine kinase (HK) and a response regulator, are the main signaling pathways in bacteria, global TCS activity remains poorly described. Here, we report the kinetic parameters of the HK autophosphorylation reaction using previously uncharacterized γ-phosphate-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00485

    authors： Chase OM,Espinasse A,Wilke KE,Carlson EE

    更新日期：2018-07-24 00:00:00

  • Interactions between model proteins and deoxyribonucleic acids.

    abstract：:Interactions between DNA and model proteins, poly(L-Lys(m)L-Ala(n)), where m + n = 100%, have been investigated using thermal denaturation and circular dichroism (CD). All complexes of DNA with these proteins precipitate in a small range of input ratios, protein to DNA, with the midpoints of all precipitation curves c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00653a014

    authors： Pinkston MF,Ritter AH,Li HJ

    更新日期：1976-04-20 00:00:00

  • Mechanism of transfer of reduced nicotinamide adenine dinucleotide among dehydrogenases. Transfer rates and equilibria with enzyme-enzyme complexes.

    abstract：:The direct transfer of NADH between A-B pairs of dehydrogenases and also the dissociation of NADH from individual E-NADH complexes have been investigated by transient stopped-flow kinetic techniques. Such A-B transfers of NADH occur without the intermediate dissociation of coenzyme into the aqueous solvent environment...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00379a006

    authors： Srivastava DK,Bernhard SA

    更新日期：1987-03-10 00:00:00