Exploration of the Effects of γ-Phosphate-Modified ATP Analogues on Histidine Kinase Autophosphorylation.

abstract：:Malate dehydrogenase occurs in virtually all eucaryotic cells in mitochondrial and cytoplasmic forms, both of which are composed of two identical subunits. The reactivation of the mitochondrial isoenzyme has been the subject of previous studies [Jaenicke, R., Rudolph, R., & Heider, I. (1979) Biochemistry 18, 1217-1223...

journal_title：Biochemistry

authors： Rudolph R,Fuchs I,Jaenicke R

更新日期：1986-04-08 00:00:00

abstract：:The structure of human epidermal growth factor (EGF, 53 amino acids) comprises three distinct loops (A, B, and C) connected correspondingly by the three native disulfide bonds, Cys(6)-Cys(20), Cys(14)-Cys(31), and Cys(33)-Cys(42). The connection of Cys(6) and Cys(20) forming the N-terminal A loop is essential for the ...

journal_title：Biochemistry

authors： Lu BY,Jiang C,Chang JY

更新日期：2005-11-15 00:00:00

abstract：:For the class A β-lactamase SHV-1, the kinetic and mechanistic properties of the clinically used inhibitor sulbactam are compared with the sulbactam analog substituted in its 6β position by a CH2OH group (6β-(hydroxymethyl)penicillanic acid). The 6β substitution improves both in vitro and microbiological inhibitory pr...

journal_title：Biochemistry

authors： Che T,Rodkey EA,Bethel CR,Shanmugam S,Ding Z,Pusztai-Carey M,Nottingham M,Chai W,Buynak JD,Bonomo RA,van den Akker F,Carey PR

更新日期：2015-01-27 00:00:00

abstract：:Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...

journal_title：Biochemistry

authors： Arnold WR,Baylon JL,Tajkhorshid E,Das A

更新日期：2017-12-26 00:00:00

abstract：:Fluid-phase interactions between hematologic cells and those of the vessel wall were studied in order to define a role for lipoxygenase products as cell signals in the control of vascular cholesterol metabolism. A functional parameter for hydroxy acids in this system has not been previously demonstrated. We report her...

journal_title：Biochemistry

authors： Hajjar DP,Marcus AJ,Etingin OR

更新日期：1989-10-31 00:00:00

abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

journal_title：Biochemistry

authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

更新日期：1999-01-05 00:00:00

abstract：:Heme oxygenase-1 (HO-1) is an enzyme that catalyzes the oxidative degradation of heme. Since free heme is toxic to cells, rapid degradation of heme is important for maintaining cellular health. There have been useful mechanistic studies of the HO reaction based on crystal structures; however, how HO-1 recognizes heme ...

journal_title：Biochemistry

authors： Harada E,Sugishima M,Harada J,Fukuyama K,Sugase K

更新日期：2015-01-20 00:00:00

abstract：:Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...

journal_title：Biochemistry

authors： St-Jean M,Blonski C,Sygusch J

更新日期：2009-06-02 00:00:00

abstract：:In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...

journal_title：Biochemistry

authors： Munson LM,Reznikoff WS

更新日期：1981-04-14 00:00:00

abstract：:Human DNA primase synthesizes short RNA primers that DNA polymerase alpha then elongates during the initiation of all new DNA strands. Even though primase misincorporates NTPs at a relatively high frequency, this likely does not impact the final DNA product since the RNA primer is replaced with DNA. We used an extensi...

journal_title：Biochemistry

authors： Urban M,Joubert N,Purse BW,Hocek M,Kuchta RD

更新日期：2010-02-02 00:00:00

abstract：:A general protocol for preparation of oligonucleotides containing intrastrand cross-links between the exocyclic amino groups of adjacent deoxyguanosines has been developed. A series of 2, 3, and 4 methylene cross-links was incorporated site-specifically into an 11-mer (5'-GGCAGGTGGTG-3', cross-linked positions are und...

journal_title：Biochemistry

authors： Kowalczyk A,Carmical JR,Zou Y,Van Houten B,Lloyd RS,Harris CM,Harris TM

更新日期：2002-03-05 00:00:00

abstract：:We show here that expression of chromogranins in non-neuroendocrine NIH3T3 cells significantly increased the amount of IP(3)-mediated intracellular Ca(2+) mobilization in these cells, whereas suppression of them in neuroendocrine PC12 cells decreased the amount of mobilized Ca(2+). We have therefore investigated the r...

journal_title：Biochemistry

authors： Huh YH,Jeon SH,Yoo JA,Park SY,Yoo SH

更新日期：2005-04-26 00:00:00

abstract：:The kinetics of formation of the ADP-sensitive (EP) and ADP-insensitive (E*P) phosphoenzyme intermediates of the CaATPase in sarcoplasmic reticulum (SR) were investigated by means of the quenched-flow technique. At 21 degrees C, addition of saturating ADP to SR vesicles phosphorylated for 116 ms with 10 microM ATP gav...

journal_title：Biochemistry

authors： Froehlich JP,Heller PF

更新日期：1985-01-01 00:00:00

abstract：:Calmodulin (CaM) is a ubiquitous protein in nature and plays a regulatory role in numerous biological processes, including the upregulation of nitric oxide (NO) synthesis in vivo. Several peptides that prevent NO production by interacting with CaM have been isolated in the cutaneous secretions of Australian amphibians...

journal_title：Biochemistry

authors： Calabrese AN,Bowie JH,Pukala TL

更新日期：2015-01-20 00:00:00

abstract：:DNA gyrase and topoisomerase IV (Topo IV) are cellular targets of quinolone antibacterial drugs. The Ser-80 and the Glu-84 of the ParC subunit have been identified as mutational hotspots for quinolone resistance. Mutant Topo IV proteins containing a quinolone resistance-conferring mutation have been constructed, and t...

journal_title：Biochemistry

authors： Hiasa H

更新日期：2002-10-01 00:00:00

abstract：:Illuminating Ca2+-depleted photosystem (PS) II membranes generated two new EPR signals at g = 11 and 15 by perpendicular and parallel polarization modes, respectively. Two turnovers of the oxygen evolving center (OEC) beyond the modified S2' state are required for the appearance of these signals. The formation of the ...

journal_title：Biochemistry

authors： Mino H,Kawamori A,Matsukawa T,Ono T

更新日期：1998-03-03 00:00:00

abstract：:T4 polynucleotide kinase is widely used for 5'-phosphorylation of DNA and RNA oligonucleotide termini, but no natural protein enzyme is capable of 3'-phosphorylation. Here, we report the in vitro selection of deoxyribozymes (DNA enzymes) capable of DNA oligonucleotide 3'-phosphorylation, using a 5'-triphosphorylated R...

journal_title：Biochemistry

authors： Camden AJ,Walsh SM,Suk SH,Silverman SK

更新日期：2016-05-10 00:00:00

abstract：:In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...

journal_title：Biochemistry

authors： Suzuki A,Ito T

更新日期：1996-04-23 00:00:00

abstract：:Oxidative damage by transition metals bound to proteins may be an important pathogenic mechanism. Ceruloplasmin (Cp) is a Cu-containing plasma protein thought to be involved in oxidative modification of lipoproteins. We have previously shown that Cp increased cell-mediated low-density lipoprotein (LDL) oxidation by a ...

journal_title：Biochemistry

authors： Mukhopadhyay CK,Fox PL

更新日期：1998-10-06 00:00:00

abstract：:The chicken and goose egg white lysozymes (ChEWL and GoEWL) are homologues, but differ in substrate specificity. ChEWL catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while GoEWL is specific for bacterial peptidoglycans. The active-site aspartate 52 residue o...

journal_title：Biochemistry

authors： Matsumura I,Kirsch JF

更新日期：1996-02-13 00:00:00

abstract：:Photolyzed rhodopsin is phosphorylated at multiple serine and threonine residues during the quenching of phototransduction. Sites of phosphorylation by rhodopsin kinase have been localized to the C-terminal region of rhodopsin, but no information was available on the kinetics and identity of phosphorylated residues. T...

journal_title：Biochemistry

authors： Ohguro H,Palczewski K,Ericsson LH,Walsh KA,Johnson RS

更新日期：1993-06-01 00:00:00

abstract：:A role for Tyr254 in L-lactate dehydrogenation catalyzed by flavocytochrome b2 has recently been proposed on the basis of the known active-site structure and of studies that had suggested a mechanism involving the initial formation of a lactate carbanion [Lederer, F., & Mathews, F.S. (1987) in Flavins and Flavoprotein...

journal_title：Biochemistry

authors： Dubois J,Chapman SK,Mathews FS,Reid GA,Lederer F

更新日期：1990-07-10 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...

journal_title：Biochemistry

authors： Danyal K,Dean DR,Hoffman BM,Seefeldt LC

更新日期：2011-11-01 00:00:00

abstract：:Recognition for proteolysis by trypsin depends almost exclusively on tight binding of arginine or lysine side chains by the primary substrate specificity pocket. Although extended subsite interactions are important for catalysis, the majority of binding energy is localized in the P1 pocket. Analysis of the interaction...

journal_title：Biochemistry

authors： Willett WS,Brinen LS,Fletterick RJ,Craik CS

更新日期：1996-05-14 00:00:00

abstract：:Pig pancreatic alpha-amylase (PPA), an enzyme belonging to the alpha-amylase family, is involved in the degradation of starch. Like some other members of this family, PPA requires chloride to reach maximum activity levels. To further explain the mechanism of chloride activation, a crystal of wild-type PPA soaked with ...

journal_title：Biochemistry

authors： Qian M,Ajandouz el H,Payan F,Nahoum V

更新日期：2005-03-08 00:00:00

abstract：:The acetoacetate decarboxylase-like superfamily (ADCSF) is a little-explored group of enzymes that may contain new biocatalysts. The low level of sequence identity (∼20%) between many ADCSF enzymes and the confirmed acetoacetate decarboxylases led us to investigate the degree of diversity in the reaction and substrate...

journal_title：Biochemistry

authors： Mydy LS,Hoppe RW,Hagemann TM,Schwabacher AW,Silvaggi NR

更新日期：2019-10-08 00:00:00

abstract：:Soybean lipoxygenase 1 was studied using limited proteolysis and active-site labeling to begin the structural characterization of the enzyme in solution. The serine proteases trypsin and chymotrypsin cleaved the large monomeric protein (95 kDa) into two large polypeptides, a C-terminal fragment of about 30 kDa and an ...

journal_title：Biochemistry

authors： Ramachandran S,Carroll RT,Dunham WR,Funk MO Jr

更新日期：1992-08-25 00:00:00

abstract：:Despite recognition that the plasma membrane (PM) is comprised of lipid raft domains that are key organizing sites of multiple signaling pathways and other cell functions, limited information is available regarding the structure and function in sterol dynamics of these microdomains. To begin to resolve these issues, M...

journal_title：Biochemistry

authors： Gallegos AM,Storey SM,Kier AB,Schroeder F,Ball JM

更新日期：2006-10-03 00:00:00

abstract：:A detailed interpretation of the grossly distorted ultraviolet absorption and circular dichroism spectra of the intact red blood cell is given, including an evaluation of the effects of protein conformation, detector geometry, cell hemoglobin content, and refractive index on the calculated cell spectra. The origins of...

journal_title：Biochemistry

authors： Gitter-Amir A,Schneider AS,Rosenheck K

更新日期：1976-07-13 00:00:00

abstract：:Biotin synthase, a member of the "radical SAM" family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin (DTB). The active form of the enzyme contains two iron-sulfur clusters, a [4Fe-4S](2+) cluster liganded by Cys-53, Cys-57, and Cys-60 and the S-ad...

journal_title：Biochemistry

authors： Lotierzo M,Raux E,Tse Sum Bui B,Goasdoue N,Libot F,Florentin D,Warren MJ,Marquet A

更新日期：2006-10-10 00:00:00