DNA Oligonucleotide 3'-Phosphorylation by a DNA Enzyme.

abstract：:Ligand-dependent site-site (or subunit-subunit) interactions provide the basis for explaining cooperativity in chemical reactions. Even in the simplest possible nonaggregating system, interpretation of the interactions in terms of structural details requires an explicit assumption (or model) for the binding of the lig...

journal_title：Biochemistry

authors： Saroff HA

更新日期：1991-10-22 00:00:00

abstract：:High-precision studies of oxygen binding in hemoglobin (HbA0) solutions at near-physiological concentrations (2-12 mM heme; pHs 7.0-9.1; various buffers) have led to an unanticipated result: an unmeasurably low contribution from the triply ligated species. We have obtained this result from new differential oxygen-bind...

journal_title：Biochemistry

authors： Gill SJ,Di Cera E,Doyle ML,Bishop GA,Robert CH

更新日期：1987-06-30 00:00:00

abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...

journal_title：Biochemistry

authors： Allocatelli CT,Cutruzzolà F,Brancaccio A,Brunori M,Qin J,La Mar GN

更新日期：1993-06-15 00:00:00

abstract：:We report an unusually high frequency (543 cm(-)(1)) for an Fe-CO stretching mode in the CO complex of Ascaris suum hemoglobin as compared to vertebrate hemoglobins in which the frequency of the Fe-CO mode is much lower. A second Fe-CO stretching mode in Ascaris hemoglobin is observed at 515 cm(-1). We propose that th...

journal_title：Biochemistry

authors： Das TK,Friedman JM,Kloek AP,Goldberg DE,Rousseau DL

更新日期：2000-02-01 00:00:00

abstract：:The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistr...

journal_title：Biochemistry

authors： Hofmann K,Zhang WJ,Romovacek H,Finn FM,Bothner-By AA,Mishra PK

更新日期：1984-06-05 00:00:00

abstract：:We report differences in the interaction of two structurally similar phenanthroline intercalators, ethidium and propidium, with poly(dA).poly(dT) and poly[d(A-T)] as a function of ionic strength based on titration microcalorimetry, fluorescence titration, and hydrostatic pressure measurements. Both ethidium and propid...

journal_title：Biochemistry

authors： Marky LA,Macgregor RB Jr

更新日期：1990-05-22 00:00:00

abstract：:Little is known about how a membrane can regulate interactions between transmembrane helices. Here, we show that strong self-interaction of the transmembrane helix of human quiescin sulfhydryl oxidase 2 rests on a motif of conserved amino acids comprising one face of the helix. Atomistic molecular dynamics simulations...

journal_title：Biochemistry

authors： Ried CL,Scharnagl C,Langosch D

更新日期：2016-03-08 00:00:00

abstract：:The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...

journal_title：Biochemistry

authors： Shui X,Sines CC,McFail-Isom L,VanDerveer D,Williams LD

更新日期：1998-12-01 00:00:00

abstract：:The nucleotide binding site of the uncoupling protein (UCP) from brown adipose tissue was mapped by photoaffinity labeling with 2-azidoadenosine 5'-triphosphate (2-azido-ATP) and by affinity labeling with 3'-O-(5-fluoro-2,4-dinitrophenyl)adenosine 5'-triphosphate (FDNP-ATP). Both analogs bind with high affinity and sp...

journal_title：Biochemistry

authors： Mayinger P,Klingenberg M

更新日期：1992-11-03 00:00:00

abstract：:Nicotinic acetylcholine receptors (nAChRs) were studied in detail in the past regarding their interaction with therapeutic and drug addiction related compounds. Using fast kinetic whole-cell recording, we have now studied effects of tacrine, an agent used clinically to treat Alzheimer's disease, on currents elicited b...

journal_title：Biochemistry

authors： Cheffer A,Ulrich H

更新日期：2011-03-22 00:00:00

abstract：:Although point mutations usually lead to minor localized changes in protein structure, replacement of conserved Pro-76 with Gly in iso-2-cytochrome c induces a major conformational change. The change in structure results from mutation-induced depression of the pK for transition to an alkaline conformation with altered...

journal_title：Biochemistry

authors： Nall BT,Zuniga EH,White TB,Wood LC,Ramdas L

更新日期：1989-12-12 00:00:00

abstract：:A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...

journal_title：Biochemistry

authors： Volwerk JJ,Mrsny RJ,Patapoff TW,Jost PC,Griffith OH

更新日期：1987-01-27 00:00:00

abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

journal_title：Biochemistry

authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

更新日期：1989-05-30 00:00:00

abstract：:In the presence of Ca2+ and calmodulin (CM), purified smooth muscle myosin light chain kinase (MLCKase) was found to undergo autophosphorylation at a rate that was about 200-fold slower than its catalytic activity. Up to 1.7 mol of phosphate were incorporated per mole of kinase. Lower levels of incorporation could be ...

journal_title：Biochemistry

authors： Sobieszek A

更新日期：1995-09-19 00:00:00

abstract：:Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...

journal_title：Biochemistry

authors： Davey MW,Sulkowski E,Carter WA

更新日期：1976-02-10 00:00:00

abstract：:To elucidate early stages in protein folding, we have adopted a fragment reconstitution method for small proteins. This approach is expected to provide nuclei for protein folding and to allow us to investigate folding mechanisms. In previous work [Kobayashi, N., et al. (1995) FEBS Lett. 366, 99-103.] we demonstrated t...

journal_title：Biochemistry

authors： Honda S,Kobayashi N,Munekata E,Uedaira H

更新日期：1999-01-26 00:00:00

abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...

journal_title：Biochemistry

authors： Asuru AP,An M,Busenlehner LS

更新日期：2012-09-11 00:00:00

abstract：:Several naturally occurring dietary polyphenols with chemopreventive or anticancer properties are topoisomerase II poisons. To identify additional phytochemicals that enhance topoisomerase II-mediated DNA cleavage, a library of 341 Mediterranean plant extracts was screened for activity against human topoisomerase IIα....

journal_title：Biochemistry

authors： Vann KR,Sedgeman CA,Gopas J,Golan-Goldhirsh A,Osheroff N

更新日期：2015-07-28 00:00:00

abstract：:Although enzymes of thermophilic organisms are often very resistant to thermal denaturation, they are usually less active than their mesophilic or psychrophilic homologues at moderate or low temperatures. To explore the structural features that would improve the activity of a thermophilic enzyme at less than optimal t...

journal_title：Biochemistry

authors： Hayashi S,Akanuma S,Onuki W,Tokunaga C,Yamagishi A

更新日期：2011-10-11 00:00:00

abstract：:Guided by the X-ray structure analysis of a crystalline EcoRV-d(GGGATATCCC) complex (Winkler, in preparation), we have begun to identify functionally important amino acid residues of EcoRV. We show here that Asn70, Asp74, Ser183, Asn185, Thr186, and Asn188 are most likely involved in the binding and/or cleavage of the...

journal_title：Biochemistry

authors： Thielking V,Selent U,Köhler E,Wolfes H,Pieper U,Geiger R,Urbanke C,Winkler FK,Pingoud A

更新日期：1991-07-02 00:00:00

abstract：:Novel inhibitors of lupin diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) hydrolase have been identified by in silico screening of a large virtual chemical library. Compounds were ranked on the basis of a consensus from six scoring functions. From the top 100 ranked compounds six were selected and initially scre...

journal_title：Biochemistry

authors： Branson KM,Mertens HD,Swarbrick JD,Fletcher JI,Kedzierski L,Gayler KR,Gooley PR,Smith BJ

更新日期：2009-08-18 00:00:00

abstract：:Listeriolysin O (LLO) is the most important virulence factor of the intracellular pathogen Listeria monocytogenes. Its main task is to enable escape of bacteria from the phagosomal vacuole into the cytoplasm. LLO belongs to the cholesterol-dependent cytolysin (CDC) family but differs from other members, as it exhibits...

journal_title：Biochemistry

authors： Bavdek A,Gekara NO,Priselac D,Gutiérrez Aguirre I,Darji A,Chakraborty T,Macek P,Lakey JH,Weiss S,Anderluh G

更新日期：2007-04-10 00:00:00

abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...

journal_title：Biochemistry

authors： Cotruvo JA,Stubbe J

更新日期：2011-03-15 00:00:00

abstract：:PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...

journal_title：Biochemistry

authors： Jiang P,Ninfa AJ

更新日期：2009-12-08 00:00:00

abstract：:5-HT(2A) serotonin receptors represent the principal molecular targets for LSD-like hallucinogens and atypical antipsychotic drugs. It has been proposed that a dysregulation of 5-HT(2A) receptor-mediated signaling may contribute to the pathogenesis of schizophrenia and related diseases. A major mechanism for the atten...

journal_title：Biochemistry

authors： Gray JA,Compton-Toth BA,Roth BL

更新日期：2003-09-16 00:00:00

abstract：:Retinitis pigmentosa (RP), a neurodegenerative disorder, can arise from single point mutations in rhodopsin, leading to a cascade of protein instability, misfolding, aggregation, rod cell death, retinal degeneration, and ultimately blindness. Divalent cations, such as zinc and copper, have allosteric effects on misfol...

journal_title：Biochemistry

authors： Gleim S,Stojanovic A,Arehart E,Byington D,Hwa J

更新日期：2009-03-03 00:00:00

abstract：:Mouse fibroblasts contain a macromolecular binding component (receptor) which binds glucocorticoids specifically and with high affinity. This study shows that there are three different cellular forms of bound receptor and that it is experimentally possible to markedly alter the subcellular distribution of these three ...

journal_title：Biochemistry

authors： Middlebrook JL,Wong MD,Ishii DN,Aronow L

更新日期：1975-01-14 00:00:00

abstract：:The three-dimensional structure of toxic shock syndrome toxin 1 (TSST-1) from Staphylococcus aureus has been determined and refined to an R value of 0.226 for data between 8- and 2.5-A resolution. Overall, the structure of TSST-1 is similar to that of another superantigen, staphylococcal enterotoxin B (SEB). The key d...

journal_title：Biochemistry

authors： Prasad GS,Earhart CA,Murray DL,Novick RP,Schlievert PM,Ohlendorf DH

更新日期：1993-12-21 00:00:00

abstract：:Carbon monoxide dehydrogenase (CODH) from Clostridium thermoaceticum plays a central role in the newly discovered acetyl-CoA pathway [Wood, H.G., Ragsdale, S.W., & Pezacka, E. (1986) FEMS Microbiol. Rev. 39, 345-362]. The enzyme catalyzes the formation of acetyl-CoA from methyl, carbonyl, and CoA groups, and it has sp...

journal_title：Biochemistry

authors： Shanmugasundaram T,Kumar GK,Wood HG

更新日期：1988-08-23 00:00:00

abstract：:The aminoacylation kinetics of 19 different variants of yeast tRNATyr with nucleotide substitutions in positions 33-35 were determined. Substitution of the conserved uridine-33 does not alter the rate of aminoacylation. However, substitution of the anticodon position 34 or position 35 reduces Km from 2- to 10-fold and...

journal_title：Biochemistry

authors： Bare LA,Uhlenbeck OC

更新日期：1986-09-23 00:00:00