Sterol and pH interdependence in the binding, oligomerization, and pore formation of Listeriolysin O.

abstract：:PsbO, the photosystem II manganese stabilizing protein, contains an aspartate residue [Asp157 (spinach numbering)], which is highly conserved in eukaryotic and prokaryotic PsbOs. The homology model of the PSII-bound conformation of spinach PsbO presented here positions Asp157 in the large flexible loop of the protein....

journal_title：Biochemistry

authors： Popelkova H,Commet A,Yocum CF

更新日期：2009-12-22 00:00:00

abstract：:The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...

journal_title：Biochemistry

authors： Stetler-Stevenson WG,Veis A

更新日期：1983-08-30 00:00:00

abstract：:The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...

journal_title：Biochemistry

authors： Cane DE,Chiu HT,Liang PH,Anderson KS

更新日期：1997-07-08 00:00:00

abstract：:Under suitable experimental conditions the aerobic oxidation of NADH catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. A trace amount of H2O2 present in the NADH solution brought about initial burst in the formation of oxyperoxida...

journal_title：Biochemistry

authors： Yokota K,Yamazaki I

更新日期：1977-05-03 00:00:00

abstract：:Apolipoprotein (apo) A-I, a 243-residue, 28.1-kDa protein is a major mediator of the reverse cholesterol transport (RCT) pathway, a process that may reduce the risk of cardiovascular disease in humans. In plasma, a small fraction of lipid-free or lipid-poor apoA-I is likely a key player in the first step of RCT. There...

journal_title：Biochemistry

authors： Silva RA,Hilliard GM,Fang J,Macha S,Davidson WS

更新日期：2005-03-01 00:00:00

abstract：:The synaptotagmin (Syt) family of proteins plays an important role in vesicle docking and fusion during Ca(2+)-induced exocytosis in a wide variety of cell types. Its role as a Ca(2+) sensor derives primarily from its two C2 domains, C2A and C2B, which insert into anionic lipid membranes upon binding Ca(2+). Syt isofo...

journal_title：Biochemistry

authors： Osterberg JR,Chon NL,Boo A,Maynard FA,Lin H,Knight JD

更新日期：2015-09-22 00:00:00

abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...

journal_title：Biochemistry

authors： Zhu Z,Becklin RR,Desiderio DM,Dalton JT

更新日期：2001-09-11 00:00:00

abstract：:The skeletal muscle Ca2+ release channel (RYR1) is regulated by calmodulin in both its Ca2+-free (apocalmodulin) and Ca2+-bound (Ca2+ calmodulin) states. Apocalmodulin is an activator of the channel, and Ca2+ calmodulin is an inhibitor of the channel. Both apocalmodulin and Ca2+ calmodulin binding sites on RYR1 are de...

journal_title：Biochemistry

authors： Moore CP,Rodney G,Zhang JZ,Santacruz-Toloza L,Strasburg G,Hamilton SL

更新日期：1999-06-29 00:00:00

abstract：:Simultaneous replacement of Asp-94 with serine and Gly-98 with glutamate in rat alpha-parvalbumin creates a CD-site ligand array in the context of the EF-site binding loop. Previous work has shown that, relative to the wild-type CD site, this engineered site has markedly reduced Ca(2+) affinity. Seeking an explanation...

journal_title：Biochemistry

authors： Tanner JJ,Agah S,Lee YH,Henzl MT

更新日期：2005-08-23 00:00:00

abstract：:Human and mouse metallothionein-3 (MT-3) molecules exhibit the same metal binding stoichiometry with Zn(II), Cd(II), or Cu(I) as MT-1 or MT-2 molecules, suggesting that MT-3 consists of two domains enfolding separate polymetallic clusters. The kinetic reactivities of Zn(II) complexes of MT-3 with the chelator ethylene...

journal_title：Biochemistry

authors： Sewell AK,Jensen LT,Erickson JC,Palmiter RD,Winge DR

更新日期：1995-04-11 00:00:00

abstract：:Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences...

journal_title：Biochemistry

authors： Jain NU,Tjioe E,Savidor A,Boulie J

更新日期：2005-06-28 00:00:00

abstract：:The structure of a synthetic peptide corresponding to residues 1-45 of rabbit muscle adenylate kinase has been studied in aqueous solution by two-dimensional NMR, FTIR, and CD spectroscopy. This peptide, which binds MgATP and is believed to represent most of the MgATP-binding site of the enzyme [Fry, D.C., Kuby, S.A.,...

journal_title：Biochemistry

authors： Fry DC,Byler DM,Susi H,Brown EM,Kuby SA,Mildvan AS

更新日期：1988-05-17 00:00:00

abstract：:We have previously suggested that folding of the SV40 attenuator RNA 1 into two hairpin elements leads to a block of transcription elongation. Using site-directed mutagenesis, we created three templates that either strengthened or weakened the proposed hairpin structures. The mutated and wild-type templates were clone...

journal_title：Biochemistry

authors： Goldring NB,Kessler M,Aloni Y

更新日期：1992-09-08 00:00:00

abstract：:Phosphofructokinase from Escherichia coli (EcPFK) is a homotetramer with four active sites and four allosteric sites. Understanding the allosteric activation of EcPFK by MgADP has been complicated by the complex web of possible interactions, including active site homotropic interactions, allosteric site homotropic int...

journal_title：Biochemistry

authors： Fenton AW,Paricharttanakul NM,Reinhart GD

更新日期：2004-11-09 00:00:00

abstract：:S100 beta is a calcium-binding protein, which regulates the activities of several enzymes and inhibits the phosphorylation of a variety of protein kinase C substrates in a calcium-dependent manner. The protein was recently found to activate a retinal membrane guanylate cyclase, and in this paper, we report that it inh...

journal_title：Biochemistry

authors： Pozdnyakov N,Margulis A,Sitaramayya A

更新日期：1998-07-28 00:00:00

abstract：:Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...

journal_title：Biochemistry

authors： Buchko GW,Tarasevich BJ,Bekhazi J,Snead ML,Shaw WJ

更新日期：2008-12-16 00:00:00

abstract：:The crystal structure of the recombinant thiamin diphosphate-dependent E1 component from the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) has been determined at a resolution of 1.85 A. The E. coli PDHc E1 component E1p is a homodimeric enzyme and crystallizes with an intact dimer in an asymmetric...

journal_title：Biochemistry

authors： Arjunan P,Nemeria N,Brunskill A,Chandrasekhar K,Sax M,Yan Y,Jordan F,Guest JR,Furey W

更新日期：2002-04-23 00:00:00

abstract：:Polyalanine (polyA) is the third-most prevalent homopeptide repeat in eukaryotes, behind polyglutamine and polyasparagine. Abnormal expansion of the polyA repeat is linked to at least nine human diseases, and the disease mechanism likely involves enhanced length-dependent aggregation. Because of the simplicity of its ...

journal_title：Biochemistry

authors： Bernacki JP,Murphy RM

更新日期：2011-11-01 00:00:00

abstract：:Creatininase catalyzes the conversion of creatinine (a biosensor for kidney function) to creatine via a two-step mechanism: water addition followed by ring opening. Water addition is common to other known cyclic amidohydrolases, but the precise mechanism for ring opening is still under debate. The proton donor in this...

journal_title：Biochemistry

authors： Jitonnom J,Mujika JI,van der Kamp MW,Mulholland AJ

更新日期：2017-12-05 00:00:00

abstract：:PsbU is a lumenal peripheral protein in the photosystem II (PS II) complex of cyanobacteria and red algae. It is thought that PsbU is replaced functionally by PsbP or PsbQ in plant chloroplasts. After the discovery of PsbP and PsbQ homologues in cyanobacterial PS II [Thornton et al. (2004) Plant Cell 16, 2164-2175], w...

journal_title：Biochemistry

authors： Inoue-Kashino N,Kashino Y,Satoh K,Terashima I,Pakrasi HB

更新日期：2005-09-13 00:00:00

abstract：:We have measured the frequency of the carbon-hydrogen stretching mode of the pro-R and pro-S C4-H bonds of NADH in solution and when bound to pig heart lactate (LDH) or mitochondrial malate (mMDH) dehydrogenases. This is achieved by specifically deuterating the C4 pro-R or pro-S hydrogens of NADH and determining the f...

journal_title：Biochemistry

authors： Deng H,Zheng J,Sloan D,Burgner J,Callender R

更新日期：1992-06-02 00:00:00

abstract：:Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...

journal_title：Biochemistry

authors： Cogoi S,Codognotto A,Rapozzi V,Meeuwenoord N,van der Marel G,Xodo LE

更新日期：2005-08-09 00:00:00

abstract：:The role of the Na,K-ATPase beta-subunit in stabilization of ion-binding sites has been investigated. Treatment of the purified renal Na,K-ATPase with 0.25 M DTT at 40 degrees C for 1 h resulted in 50% loss of Rb occlusion, which correlates with partial reduction of S-S bridges in the extracellular portion of the beta...

journal_title：Biochemistry

authors： Lutsenko S,Kaplan JH

更新日期：1993-07-06 00:00:00

abstract：:The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...

journal_title：Biochemistry

authors： Hyland LJ,Tomaszek TA Jr,Roberts GD,Carr SA,Magaard VW,Bryan HL,Fakhoury SA,Moore ML,Minnich MD,Culp JS

更新日期：1991-08-27 00:00:00

abstract：:The purified ATPase of sarcoplasmic reticulum was covalently labeled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-IAEDANS) or with iodoacetamidofluorescein (IAF). In reconstituted vesicles containing both types of ATPase molecules fluorescence energy transfer was observed from the IAEDANS (donor) to t...

journal_title：Biochemistry

authors： Vanderkooi JM,Ierokomas A,Nakamura H,Martonosi A

更新日期：1977-04-05 00:00:00

abstract：:Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...

journal_title：Biochemistry

authors： Zhao B,Lamb DC,Lei L,Kelly SL,Yuan H,Hachey DL,Waterman MR

更新日期：2007-07-31 00:00:00

abstract：:Substitution of cysteine for proline-39 in Escherichia coli dihydrofolate reductase by oligonucleotide-directed mutagenesis positions the new cysteine adjacent to already existing cysteine-85. When the mutant protein is expressed in the E. coli cytosol, the cysteine sulfur atoms are found, by X-ray crystallographic an...

journal_title：Biochemistry

authors： Villafranca JE,Howell EE,Oatley SJ,Xuong NH,Kraut J

更新日期：1987-04-21 00:00:00

abstract：:The ileal apical and liver basolateral bile acid transporters catalyze the Na+-dependent uptake of these amphipathic molecules in the intestine and liver. They contain nine predicted helical hydrophobic sequences (H1-H9) between the exoplasmic N-glycosylated N terminus and the cytoplasmic C terminus. Previous in vitro...

journal_title：Biochemistry

authors： Mareninova O,Shin JM,Vagin O,Turdikulova S,Hallen S,Sachs G

更新日期：2005-10-25 00:00:00

abstract：:βγ-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, βγ-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has ...

journal_title：Biochemistry

authors： Srivastava SS,Jamkhindikar AA,Raman R,Jobby MK,Chadalawada S,Sankaranarayanan R,Sharma Y

更新日期：2017-03-07 00:00:00

abstract：:Two extremely potent inhibitors of citrate synthase, carboxyl and primary amide analogues of acetyl coenzyme A, have been synthesized. The ternary complexes of these inhibitors with oxaloacetate and citrate synthase have been crystallized and their structures analyzed at 1.70- and 1.65-A resolution, respectively. The ...

journal_title：Biochemistry

authors： Usher KC,Remington SJ,Martin DP,Drueckhammer DG

更新日期：1994-06-28 00:00:00