Abstract:
:The crystal structure of the recombinant thiamin diphosphate-dependent E1 component from the Escherichia coli pyruvate dehydrogenase multienzyme complex (PDHc) has been determined at a resolution of 1.85 A. The E. coli PDHc E1 component E1p is a homodimeric enzyme and crystallizes with an intact dimer in an asymmetric unit. Each E1p subunit consists of three domains: N-terminal, middle, and C-terminal, with all having alpha/beta folds. The functional dimer contains two catalytic centers located at the interface between subunits. The ThDP cofactors are bound in the "V" conformation in clefts between the two subunits (binding involves the N-terminal and middle domains), and there is a common ThDP binding fold. The cofactors are completely buried, as only the C2 atoms are accessible from solution through the active site clefts. Significant structural differences are observed between individual domains of E1p relative to heterotetrameric multienzyme complex E1 components operating on branched chain substrates. These differences may be responsible for reported alternative E1p binding modes to E2 components within the respective complexes. This paper represents the first structural example of a functional pyruvate dehydrogenase E1p component from any species. It also provides the first representative example for the entire family of homodimeric (alpha2) E1 multienzyme complex components, and should serve as a model for this class of enzymes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Arjunan P,Nemeria N,Brunskill A,Chandrasekhar K,Sax M,Yan Y,Jordan F,Guest JR,Furey Wdoi
10.1021/bi0118557subject
Has Abstractpub_date
2002-04-23 00:00:00pages
5213-21issue
16eissn
0006-2960issn
1520-4995pii
bi0118557journal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The qualitative and quantitative features of mutagenesis by two DNA adducts of carcinogenic alkylating agents, O6-methylguanine (m6G) and O4-methylthymine (m4T), were examined in vivo. The deoxyhexanucleotides 5'-GCTAGC-3' and 5'-GCTAGC-3' were synthesized, where the underlined bases are the positions of m4T or m6G, r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00242a031
更新日期:1991-07-16 00:00:00
abstract::Protease inhibition by metastable serine protease inhibitors (serpins) is mediated by one of the largest functional intradomain conformational changes known in biology. In this extensive structural rearrangement, protease-serpin complex formation triggers cleavage of the serpin reactive center loop (RCL), its subseque...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00839
更新日期:2017-01-31 00:00:00
abstract::14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00643a005
更新日期:1977-11-29 00:00:00
abstract::Cytochrome c oxidase contains four redox-active metal centers: two heme irons, cytochromes a and a3, and two copper ions, CuA and CuB. Due to the paucity of spectroscopic signatures for both copper sites in cytochrome c oxidase, the ligands and structures for these sites have remained ambiguous. The specific depletion...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00382a005
更新日期:1987-04-21 00:00:00
abstract::Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027319b
更新日期:2003-06-10 00:00:00
abstract::Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labele...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00479a012
更新日期:1990-07-10 00:00:00
abstract::The inactivation of excited rhodopsin in the presence of ATP, rhodopsin kinase, and/or arrestin has been studied from its effect on the two subsequent steps in the light-induced enzymatic cascade: metarhodopsin II catalyzed activation of G-protein and G-protein-dependent activation of cGMP phosphodiesterase. The inact...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00405a049
更新日期:1988-03-08 00:00:00
abstract::The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961770j
更新日期:1996-12-03 00:00:00
abstract::A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0001675
更新日期:2000-05-23 00:00:00
abstract::We used a combination of optical and calorimetric techniques to investigate the incorporation of deoxythymidine --> deoxyuridine (dT --> dU) substitutions in the duplex and third strand of the parallel intramolecular triplex d(A(7)C(5)T(7)C(5)T(7)) (ATT). UV and differential scanning calorimetry melting experiments sh...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052203b
更新日期:2006-03-07 00:00:00
abstract::Flash-induced Fourier transform infrared (FTIR) difference spectra for the four-step S-state cycle and the effects of global (15)N- and (13)C-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0362323
更新日期:2004-06-15 00:00:00
abstract::The temperature dependence of steady-state kinetics has been studied with horse liver alcohol dehydrogenase (HLADH) using protonated and deuterated benzyl alcohol as substrates in methanol/water mixtures between +3 and -50 degrees C. Additionally, the competitive isotope effects, k(H)/k(T) and k(D)/k(T), were measured...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002075l
更新日期:2001-02-20 00:00:00
abstract::Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedde...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.8b00944
更新日期:2019-01-29 00:00:00
abstract::The neonatal Fc receptor (FcRn) binds maternal immunoglobulin G (IgG) during the acquisition of passive immunity by the fetus or newborn. In adult mammals, FcRn also binds IgG and returns it to the bloodstream, thus protecting IgG from a default degradative pathway. Biosensor assays have been used to characterize the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970841r
更新日期:1997-08-05 00:00:00
abstract::Src homology 2 (SH2) domains are phosphotyrosine- (pY-) binding modules found in a variety of signal-transducing proteins and constitute an important class of drug targets for the treatment of signaling related diseases/conditions. To date, a large number of peptidic as well as nonpeptidic SH2 domain inhibitors have b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034076u
更新日期:2003-05-06 00:00:00
abstract::Intrastrand cross-links represent the majority of modifications in DNA resulting from interaction with the cancer chemotherapeutic drug cis-diamminedichloroplatinum(II) (cis-DDP). These adducts were recently characterized although several discrepancies remained to be resolved. In these studies, [3H]-cis-dichloro(ethyl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00361a026
更新日期:1986-07-01 00:00:00
abstract::The flexibility of individual amino acid side chains of pancreatic phospholipase A2 in aqueous and micellar solutions was studied with deuterium nuclear magnetic resonance (2H NMR). Bovine pancreatic phospholipase A2 was selectively deuterated at the aromatic ring systems of Trp-3 and Phe-5 and porcine pancreatic phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00334a029
更新日期:1985-06-18 00:00:00
abstract::Trypanosoma brucei ornithine decarboxylase was reconstituted by coexpression of two polypeptides corresponding to residues 1-305 and residues 306-425 in Escherichia coli. The two peptides were coexpressed, at wild-type levels, from a single transcriptional unit that was separated by a 15-nucleotide untranslated region...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00041a021
更新日期:1995-10-17 00:00:00
abstract::The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4005528
更新日期:2013-08-06 00:00:00
abstract::Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051377q
更新日期:2005-11-29 00:00:00
abstract::The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001276m
更新日期:2000-10-17 00:00:00
abstract::The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs. MutY also exhibits adenine glycosylase activity toward...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981594+
更新日期:1998-10-20 00:00:00
abstract::A new actinoleukin-like antitumor antibiotic, BBM-928A, has been shown to interact with isolated DNA molecules. BBM-928A contains two substituted quinolines linked by a cyclic decapeptide. Quenching effects of the covalently closed superhelical PM2 DNA on the BBM-928A fluorescence revealed a strong interaction with an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00565a012
更新日期:1980-11-25 00:00:00
abstract::The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00676a015
更新日期:1975-03-11 00:00:00
abstract::Galactosyl- and glucosylceramide, globoside, and dihydrolactosylceramide, bearing [2,2-2H2]stearic acid, have been studied at a concentration of 10 mol% in bilayers of dimyristoylphosphatidylcholine by 2H NMR. The quadrupolar splitting delta vQ of the C2 deuterons were measured at several temperatures in the range of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00232a019
更新日期:1991-05-07 00:00:00
abstract::Conjugative transfer of F-like plasmids in Escherichia coli is repressed by a plasmid-encoded protein, FinO. FinO blocks the translation of TraJ, a positive activator of transcription of genes required for conjugation. FinO binds a traJ antisense RNA, FinP, thereby protecting it from degradation, and catalyzes FinP-tr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9911482
更新日期:1999-10-19 00:00:00
abstract::To clarify the function of the hydrophilic carboxyl-terminal tail of human erythrocyte membrane band 3 protein (HEM-B3), we purified two peptides, C1 (Ala893-Val911) and KS4 (Gly647-Arg656), from human erythrocyte band 3 protein preparations. Purified C1 peptides at concentrations from 5 to 80 microM were incubated wi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035281c
更新日期:2004-02-17 00:00:00
abstract::Recent studies implicate hydrogen sulfide (H2S) oxidation as an important aspect of bacterial antibiotic resistance and sulfide homeostasis. The cst operon of the major human pathogen Staphylococcus aureus is induced by exogenous H2S stress and encodes enzymes involved in sulfide oxidation, including a group I flavopr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00714
更新日期:2016-11-29 00:00:00
abstract::Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.5b01073
更新日期:2016-03-22 00:00:00
abstract::DNA polymerase β (Pol β) is a key enzyme in mammalian base excision repair (BER), contributing stepwise 5'-deoxyribose phosphate (dRP) lyase and "gap-filling" DNA polymerase activities. The lyase reaction is believed to occur via a β-elimination reaction following the formation of a Schiff base between the dRP group a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00807
更新日期:2017-01-24 00:00:00