Abstract：
:A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through mutation at two positions is examined within a fixed structural motif. Random mutation of Phe61 and Phe65 of cytochrome b562 (E. coli), and subsequent examination of a statistically significant sampling of this library, demonstrates that the redox potential can vary over 100 mV (>25% of the known accessible potential in native proteins with axial His-Met ligation) through mutation at these two positions. The redox potential of the wild-type protein occurs at an extremum of the distribution observed, indicating that Phe61 and Phe65 were most likely naturally selected to differentially stabilize the reduced state of the protein. At the other extremum, a compositionally conservative set of mutations (F61I, F65Y) leads to a 100 mV shift in the redox equilibrium toward the oxidized state. NMR analyses indicate that a charge-dipole interaction which results from mutation of phenylalanine to tyrosine at position 65 may be responsible.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Springs SL,Bass SE,McLendon GLdoi
10.1021/bi0001675subject
Has Abstractpub_date
2000-05-23 00:00:00pages
6075-82issue
20eissn
0006-2960issn
1520-4995pii
bi0001675journal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00048a004
更新日期：1995-12-05 00:00:00
abstract：:The kinetic parameters (Km and Vmax) of sugar-modified analogues of inosine and guanosine have been determined with human erythrocytic purine nucleoside phosphorylase (PNP). Steric alterations at the 2' and 3' positions greatly lessened or abolished substrate activity. However, the 5'-deoxy- and 2',5'-dideoxy-beta-D-r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00542a016
更新日期：1980-01-08 00:00:00
abstract：:The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled nativelike functional arrays of an aspartate receptor cytoplasmic fragment (CF) with its two cytoplasmic protein partners (CheA and Ch...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00234
更新日期：2016-07-05 00:00:00
abstract：:The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700983h
更新日期：2007-11-06 00:00:00
abstract：:An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00152a009
更新日期：1992-09-22 00:00:00
abstract：:The AT-rich interaction domain (ARID) containing BAF250a is a subunit of the BAF-A class of SWI/SNF chromatin remodeling complexes. The ARID belongs to a family of conserved DNA binding domains found in several eukaryotic proteins; however, its exact contribution to BAF250a function and the mechanism of its DNA bindin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00852
更新日期：2020-01-14 00:00:00
abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802298r
更新日期：2009-02-24 00:00:00
abstract：:We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a045
更新日期：1986-05-20 00:00:00
abstract：:Isolated chloroplasts are capable of synthesizing chloroplast DNA in the presence of Mg2+ and deoxynucleoside triphosphates. The in vitro reaction proceeds for at least 60 min and is inhibited by KC1 and N-ethylmaleimide. Stretches of several hundred nucleotides in length are synthesized within an hour. Little or no i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00257a014
更新日期：1982-07-06 00:00:00
abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801047q
更新日期：2008-09-16 00:00:00
abstract：:To probe the structure and stability of the central region of lipid-free apolipoprotein (apo) A-I (residues 123-165), we studied the effects of four mutations made in this region on the conformation, stability, dimyristoylphosphatidylcholine (DMPC) binding kinetics, and size of discoidal reconstituted high-density lip...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025807d
更新日期：2002-08-20 00:00:00
abstract：:The idea of establishing the amyloid-like fibrillation tendency of pro- and antisurvival proteins of human apoptotic pathways is relevant for delineating the conditions that lead to aberrant differentiation, development, and tissue homeostasis. As the first step in this direction, we report here that the caspase recru...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900626u
更新日期：2009-08-18 00:00:00
abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300134b
更新日期：2012-04-03 00:00:00
abstract：:In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52-58]. Recently, we found that subcloned PC12h cells accumulated another un...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00414a058
更新日期：1988-07-12 00:00:00
abstract：:The specific deamidation of asparagine-71 of triosephosphate isomerase increases upon substrate binding and catalysis. This deamidation at the dimer interface initiates subunit dissociation, unfolding, and protein degradation. The apparent connection between catalysis and terminal marking supports the concept of "mole...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00188a027
更新日期：1994-06-07 00:00:00
abstract：:Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002030k
更新日期：2001-04-10 00:00:00
abstract：:Lipid-protein interactions in bovine rod outer segment disk membranes have been studied by using a series of eight stearic acid spin-label probes which were labeled at different carbon atom positions in the chain. In randomly oriented membrane dispersions, the electron spin resonance (ESR) spectra of the C-8, C-9, C-1...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00375a005
更新日期：1987-01-13 00:00:00
abstract：:The solution secondary structure of the highly active Y55F/Y88F "Tyr-14-only" mutant of delta 5-3-ketosteroid isomerase complexed with 19-nortestosterone hemisuccinate has been shown to consist of three helices, a six-stranded mixed beta-sheet, and five turns. The steroid binds near the general acid, Tyr-14, on helix ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971549m
更新日期：1997-12-02 00:00:00
abstract：:All phosphagen kinases contain a conserved cysteine residue which has been shown by crystallographic studies, on both creatine kinase and arginine kinase, to be located in the active site. There are conflicting reports as to whether this cysteine is essential for catalysis. In this study we have used site-directed mut...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011208f
更新日期：2001-10-02 00:00:00
abstract：:Affinity selection-mass spectrometry (AS-MS) screening of kinesin spindle protein (KSP) followed by enzyme inhibition studies and temperature-dependent circular dichroism (TdCD) characterization was utilized to identify a series of benzimidazole compounds. This series also binds in the presence of Ispinesib, a known a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1005283
更新日期：2010-09-28 00:00:00
abstract：:All known Mn-containing superoxide dismutases (MnSODs) have a highly conserved histidine (His-30 in Escherichia coli FeSOD) in the active-site channel, and nearly all have an active-site arginine (Arg-170) that has been proposed to play a combined structural and functional role [Chan et al., Arch. Biochem. Biophys. 27...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980167t
更新日期：1998-08-11 00:00:00
abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00201a018
更新日期：1994-09-06 00:00:00
abstract：:R67 dihydrofolate reductase (DHFR) is a novel homotetrameric protein that possesses 222 symmetry and a single, voluminous active site pore. This symmetry poses numerous limitations on catalysis; for example, two dihydrofolate (DHF) molecules or two NADPH molecules, or one substrate plus one cofactor can bind. Only the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052504l
更新日期：2006-05-30 00:00:00
abstract：:The interaction of the 20-kDa pore-forming domain of colicin A with phospholipid vesicles was investigated by gel permeation chromatography, analytical centrifugation, and electron microscopy. Under the experimental conditions of this study, this peptide was found to interact only with vesicles containing negatively c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a029
更新日期：1989-09-19 00:00:00
abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00566a019
更新日期：1980-12-09 00:00:00
abstract：:In this study, we prepared several shortened and full-length insulin analogues with substitutions at position B26. We compared the binding affinities of the analogues for rat adipose membranes with their ability to lower the plasma glucose level in nondiabetic Wistar rats in vivo after subcutaneous administration, and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702086w
更新日期：2008-05-27 00:00:00
abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981072u
更新日期：1998-11-10 00:00:00
abstract：:The influenza M2 protein is the target of the amantadine family of antiviral drugs, and its transmembrane (TM) domain structure and dynamics have been extensively studied. However, little is known about the structure of the highly conserved N-terminal ectodomain, which contains epitopes targeted by influenza vaccines....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00727
更新日期：2016-09-27 00:00:00
abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800286m
更新日期：2008-05-13 00:00:00
abstract：:The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00177
更新日期：2018-05-15 00:00:00
