Abstract:
:Flash-induced Fourier transform infrared (FTIR) difference spectra for the four-step S-state cycle and the effects of global (15)N- and (13)C-isotope labeling on the difference spectra were examined for the first time in the mid- to low-frequency (1200-800 cm(-1)) as well as the mid-frequency (1700-1200 cm(-1)) regions using photosystem (PS) II core particles from cyanobacterium Synechocystis sp. PCC 6803. The difference spectra clearly exhibited the characteristic vibrational features for each transition during the S-state cycling. It is likely that the bands that change their sign and intensity with the S-state advances reflect the changes of the amino acid residues and protein matrices that have functional and/or structural roles within the oxygen-evolving complex (OEC). Except for some minor differences, the trends of S-state dependence in the 1700-1200 cm(-1) frequency spectra of the PS II cores from Synechocystis were comparable to that of spinach, indicating that the structural changes of the polypeptide backbones and amino acid side chains that occur during the oxygen evolution are inherently identical between cyanobacteria and higher plants. Upon (13)C-labeling, most of the bands, including amide I and II modes and carboxylate stretching modes, showed downward shifts; in contrast, (15)N-labeling induced isotopic shifts that were predominantly observed in the amide II region. In the mid- to low-frequency region, several bands in the 1200-1140 cm(-1) region were attributable to the nitrogen- and/or carbon-containing group(s) that are closely related to the oxygen evolution process. Specifically, the putative histidine ligand exhibited a band at 1113 cm(-1) which was affected by both (15)N- and (13)C-labeling and showed distinct S-state dependency. The light-induced bands in the 900-800 cm(-1) region were downshifted only by (13)C-labeling, whereas the bands in the 1000-900 cm(-1) region were affected by both (15)N- and (13)C-labeling. Several modes in the mid- to low-frequency spectra were induced by the change in protonation state of the buffer molecules accompanied by S-state transitions. Our studies on the light-induced spectrum showed that contributions from the redox changes of Q(A) and the non-heme iron at the acceptor side and Y(D) were minimal. It was, therefore, suggested that the observed bands in the 1000-800 cm(-1) region include the modes of the amino acid side chains that are coupled to the oxidation of the Mn cluster. S-state-dependent changes were observed in some of the bands.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yamanari T,Kimura Y,Mizusawa N,Ishii A,Ono TAdoi
10.1021/bi0362323subject
Has Abstractpub_date
2004-06-15 00:00:00pages
7479-90issue
23eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Two thiol-activated endopeptidases with pH optima near pH 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00654a026
更新日期:1976-05-04 00:00:00
abstract::A novel single polypeptide endopeptidase of 24 kDa (24k-endopeptidase) was purified with a yield of 300-400 microg/L from conditioned medium of a bacterial strain which was identified as a new species in the genus Chryseobacterium Sp. on the basis of its 16S rDNA sequence and DNA:DNA hybridizations. The NH(2)-terminal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992014r
更新日期:2000-01-18 00:00:00
abstract::Trp151 in the lactose permease of Escherichia coli (LacY) is an important component of the sugar-binding site and the only Trp residue out of six that is in close proximity to the galactopyranoside in the structure (1PV7). The short distance between Trp151 and the sugar is favorable for Förster resonance energy transf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061632m
更新日期:2006-12-26 00:00:00
abstract::The interfacial interactions of cholesterol with sphingomyelins (SMs) containing various homogeneous acyl chains have been investigated by Langmuir film balance approaches. Low in-plane elasticity among the packed lipids was identified as an important physical feature of the cholesterol-sphingomyelin liquid-ordered ph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002791n
更新日期:2001-05-22 00:00:00
abstract::Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00182a019
更新日期:1994-04-26 00:00:00
abstract::Competitive inhibitors can activate proteases (papain, trypsin, and cathepsin S) to catalyze the synthesis of peptide bonds and accelerate the hydrolysis of poor substrates (from 1 to 99%). Reaction mixtures contained intermediate molecules that were formed by the coupling of the inhibitor with the poor substrate. Thi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061910h
更新日期:2006-12-12 00:00:00
abstract::Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100119t
更新日期:2010-06-15 00:00:00
abstract::A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020457g
更新日期:2002-11-05 00:00:00
abstract::The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00451a047
更新日期:1989-12-12 00:00:00
abstract::There is considerable evidence that mammalian beta-tubulin is phosphorylated. Specifically, of the seven beta isotypes, the phosphorylated one is beta III, the isotype found almost entirely in neurons. The phosphate is added at a serine and perhaps a tyrosine near the C-terminus. All the evidence to date has been gath...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951247p
更新日期:1996-03-26 00:00:00
abstract::Eubacterial peptidyl-tRNA hydrolase (PTH) recycles all N-blocked aminoacyl-tRNA molecules but initiator formyl-methionyl-tRNAfMet, the acceptor helix of which is characterized by a 1-72 mismatch. Positive selection by PTH of noninitiator tRNA molecules with a full 1-72 base pair is abolished, however, upon the removal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982657r
更新日期:1999-04-20 00:00:00
abstract::Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00016a013
更新日期:1995-04-25 00:00:00
abstract::Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01025
更新日期:2017-12-26 00:00:00
abstract::The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962884y
更新日期:1997-04-29 00:00:00
abstract::Nucleotide excision repair (NER) is a crucial pathway in the maintenance of genome stability requiring at least two dozen proteins. XPA and RPA have essential roles in the damage recognition step of NER. To better understand the mechanism of their interactions with DNA, we utilized equilibrium and stop-flow kinetic ap...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011041q
更新日期:2002-01-08 00:00:00
abstract::The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. The structures of the dissimilatory enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in the sulfite adduct state (FAD-sulf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0521689
更新日期:2006-03-07 00:00:00
abstract::The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00624a010
更新日期:1977-03-08 00:00:00
abstract::A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0507332
更新日期:2005-08-30 00:00:00
abstract::The quercetin binding sites on spinach chloroplast coupling factor 1 (CF1) have been investigated using direct and competitive binding, stopped-flow, temperature-jump, and fluorescence resonance energy transfer measurements. It was found that 8-anilino-1-naphthalensulfonic acid (ANS) competes with quercetin binding at...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00646a001
更新日期:1976-01-13 00:00:00
abstract::We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00196a009
更新日期:1994-08-02 00:00:00
abstract::High-resolution phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of wild-type and mutant strains of Saccharomyces cerevisiae were observed at a frequency of 145.7 MHz. Levels of various phosphorus metabolites were investigated upon addition of glucose under both aerobic and anaerobic conditions. Three mutant...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00588a006
更新日期:1979-10-16 00:00:00
abstract::Autophagy, an essential eukaryotic homeostasis pathway, allows the sequestration of unwanted, damaged, or harmful cytoplasmic components in vesicles called autophagosomes, permitting subsequent lysosomal degradation and nutrient recycling. Autophagosome nucleation is mediated by class III phosphatidylinositol-3-kinase...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00246
更新日期:2016-08-02 00:00:00
abstract::The relaxin-like factor (RLF) also named insulin-like 3 (INSL3) consists of two polypeptide chains linked by two interchain and one intrachain disulfide bond. RLF binds to its receptor (LGR8 also named RXFP2) through the B chain and initiates transmembrane communication by activating the adenylate cyclase through the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300352x
更新日期:2012-05-22 00:00:00
abstract::Lateral mobilities of fluorescent cell surface probes have been measured on normal (3T3) and transformed (SV3T3) cultured mouse fibroblasts. There is little discernible difference in the mobilities of a lipid analogue (diI), a fluorescent ganglioside derivative (GM1), and tetramethylrhodamine-labeled succinylated conc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00551a011
更新日期:1980-05-13 00:00:00
abstract::Escherichia coli DNA photolyase contains a stable flavin radical that is readily photoreduced in the presence of added electron donors. Picosecond, nanosecond, and conventional flash photolysis technique have been employed to investigate the events leading to photoreduction from 40 ps to tens of milliseconds following...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00476a008
更新日期:1990-06-19 00:00:00
abstract::The subgel phases of a homologous series of saturated straight-chain diacylphosphatidylcholines with hydrocarbon chains consisting of 10-18 carbon atoms were studied by Fourier-transform infrared spectroscopy. All of these lipids initially form a subgel phase which is spectroscopically similar to that obtained when fu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00486a024
更新日期:1990-08-28 00:00:00
abstract::A recombinant system was developed for generation of steroid-receptor complexes in vitro. The DNA- and steroid-binding domains of the rat mineralocorticoid receptor were expressed in Escherichia coli as a fusion protein with glutathione S-transferase. The identity of the expressed recombinant protein was confirmed by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a028
更新日期:1993-08-24 00:00:00
abstract::The contribution of ligand dynamics to CYP allosterism has not been considered in detail. On the basis of a previous study, we hypothesized that CYP2A6 and CYP2E1 accommodate multiple xylene ligands. As a result, the intramolecular ( k H/ k D) obs values observed for some xylene isomers are expected to be dependent on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702020y
更新日期:2008-03-04 00:00:00
abstract::Biofilms are surface-attached communities of bacteria enclosed in a polysaccharide matrix. Bacteria in a biofilm are extremely resistant to antibiotics. Several recent reports have linked the signaling molecule nitric oxide (NO) with biofilm dispersal. We have previously reported that an H-NOX (heme-nitric oxide/oxyge...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401597m
更新日期:2014-04-08 00:00:00
abstract::Glyoxylate carboligase (GCL) is a thiamin diphosphate (ThDP)-dependent enzyme, which catalyzes the decarboxylation of glyoxylate and ligation to a second molecule of glyoxylate to form tartronate semialdehyde (TSA). This enzyme is unique among ThDP enzymes in that it lacks a conserved glutamate near the N1' atom of Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300893v
更新日期:2012-10-09 00:00:00