Comparative mutagenesis of O6-methylguanine and O4-methylthymine in Escherichia coli.

abstract：:Base excision repair (BER) plays a vital role in maintaining genomic integrity in mammalian cells. DNA polymerase λ (Pol λ) is believed to play a backup role to DNA polymerase β (Pol β) in base excision repair. Two oxidized abasic lesions that are produced by a variety of DNA-damaging agents, including several antitum...

journal_title：Biochemistry

authors： Stevens AJ,Guan L,Bebenek K,Kunkel TA,Greenberg MM

更新日期：2013-02-05 00:00:00

abstract：:Guinea pig liver transglutaminase (TGase) reacts with 0.1 mM N-Cbz-L-Glu(gamma-p-nitrophenyl ester)Gly (5, prepared herein, K(M) = 0.02 mM) to undergo rapid acylation that can be followed spectrophotometrically at 400 nm (pH 7.0, 25 degrees C). Deacylation of the transiently formed thiolester acyl enzyme intermediate ...

journal_title：Biochemistry

authors： Leblanc A,Gravel C,Labelle J,Keillor JW

更新日期：2001-07-27 00:00:00

abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...

journal_title：Biochemistry

authors： Kasson PM,Rabinowitz JD,Schmitt L,Davis MM,McConnell HM

更新日期：2000-02-08 00:00:00

abstract：:Molecular cloning revealed the unique serpin endopin 2C that demonstrates selective inhibition of cathepsin L compared to papain or elastase. Endopin 2C, thus, functions as a serpin with the property of cross-class inhibition. Endopin 2C possesses homology in primary sequence to endopin 2A and other isoforms of endopi...

journal_title：Biochemistry

authors： Hwang SR,Stoka V,Turk V,Hook VY

更新日期：2005-05-31 00:00:00

abstract：:Fibronectin is composed of multiple homologous repeats and contains many functional domains. Two major heparin-binding domains have previously been identified: the Hep I site near the amino terminus and the Hep II site near the carboxyl terminus. The Hep II site has been considered the high-affinity heparin-binding si...

journal_title：Biochemistry

authors： Lin H,Lal R,Clegg DO

更新日期：2000-03-28 00:00:00

abstract：:We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...

journal_title：Biochemistry

authors： Navarro JA,Cheddar G,Tollin G

更新日期：1989-07-11 00:00:00

abstract：:Most duplex DNAs that are in the "B" conformation are not immunogenic. One important exception is poly(dG) X poly(dC), which produces a good immune response even though, by many criteria, it adopts a conventional right-handed helix. In order to investigate what features are being recognized, monoclonal antibodies were...

journal_title：Biochemistry

authors： Lee JS,Woodsworth ML,Latimer LJ

更新日期：1984-07-03 00:00:00

abstract：:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...

journal_title：Biochemistry

authors： Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

更新日期：2003-01-21 00:00:00

abstract：:Ca2+-free crystals of sarcoplasmic reticulum Ca2+-ATPase have, up until now, been obtained in the presence of inhibitors such as thapsigargin (TG), bound to the transmembrane region of this protein. Here, we examined the consequences of such binding for the protein. We found that, after TG binding, an active site liga...

journal_title：Biochemistry

authors： Montigny C,Picard M,Lenoir G,Gauron C,Toyoshima C,Champeil P

更新日期：2007-12-25 00:00:00

abstract：:We have determined the nucleotide sequence and the derived amino acid sequence of the phosphoprotein-encoding region of the chicken vitellogenin III gene. The sequence of this minor vitellogenin could be aligned with exon 22 up to exon 27 of the previously sequenced major vitellogenin II gene (van het Schip et al., 19...

journal_title：Biochemistry

authors： Byrne BM,de Jong H,Fouchier RA,Williams DL,Gruber M,Ab G

更新日期：1989-03-21 00:00:00

abstract：:The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

journal_title：Biochemistry

authors： Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

更新日期：1996-01-09 00:00:00

abstract：:In cytochrome c, the coordination of the axial Met Sδ atom to the heme Fe atom occurs in one of two distinctly different stereochemical manners, i.e., R and S configurations, depending upon which of the two lone pairs of the Sδ atom is involved in the bond; hence, the Fe-coordinated Sδ atom becomes a chiral center. In...

journal_title：Biochemistry

authors： Tai H,Tonegawa K,Shibata T,Hemmi H,Kobayashi N,Yamamoto Y

更新日期：2013-07-16 00:00:00

abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...

journal_title：Biochemistry

authors： Hazra S,Szewczak A,Ort S,Konrad M,Lavie A

更新日期：2011-04-12 00:00:00

abstract：:In the field of muscle regulation, there is still controversy as to whether Ca2+, alone, is able to shift muscle from the relaxed to the fully active state or whether cross-bridge binding also contributes to turning on muscle contraction. Our previous studies on the binding of myosin subfragment 1 (S-1) to the troponi...

journal_title：Biochemistry

authors： Williams DL Jr,Greene LE,Eisenberg E

更新日期：1988-09-06 00:00:00

abstract：:The nerve growth factors (NGF's) isolated from cobra venom (Naja naja) and mouse submaxillary gland are closely related proteins. They are structurally similar in that about 60% of their amino acid residues are identical (Hogue-Angeletti, R. A., Frazier, W. A., Jacobs, J. W., Niall, H.D., and Bradshaw, R. A. (1976), B...

journal_title：Biochemistry

authors： Server AC,Herrup K,Shooter EM,Hogue-Angeletti RA,Frazier WA,Bradshaw RA

更新日期：1976-01-13 00:00:00

abstract：:Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...

journal_title：Biochemistry

authors： Rice HC,Young-Pearse TL,Selkoe DJ

更新日期：2013-05-14 00:00:00

abstract：:Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a biopterin cofactor. Through genome sequencing, proteins that are highly homologous to the oxygenase domain of NOSs have been identified, in particular in bacteria. The active site is ...

journal_title：Biochemistry

authors： Weisslocker-Schaetzel M,Lembrouk M,Santolini J,Dorlet P

更新日期：2017-02-07 00:00:00

abstract：:The Staphylococcus aureus sortase transpeptidase SrtA isoform is responsible for the covalent attachment of virulence and colonization-associated proteins to the bacterial peptidoglycan. SrtA utilizes two substrates, undecaprenol-pyrophosphoryl-MurNAc(GlcNAc)-Ala-D-isoGlu-Lys(epsilon-Gly(5))-D-Ala-D-Ala (branched Lipi...

journal_title：Biochemistry

authors： Kruger RG,Otvos B,Frankel BA,Bentley M,Dostal P,McCafferty DG

更新日期：2004-02-17 00:00:00

abstract：:The proximity of skeletal myosin subfragment-1 (S1) to actin, and its orientation with respect to thin filaments of single muscle fibers, were compared in the presence and in the absence of ADP. The proximity was assessed by the efficiency of carbodiimide-induced cross-linking and the orientation by polarization of fl...

journal_title：Biochemistry

authors： Andreev OA,Ushakov DS,Borejdo J

更新日期：1998-12-22 00:00:00

abstract：:JZTX-XI is a peptide toxin isolated from the venom of the Chinese spider Chilobrachys jingzhao. It contains 34 residues including six cysteine residues with disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Using 3'- and 5'-RACE methods, the full-length cDNA was identified as encoding an 86-residue pr...

journal_title：Biochemistry

authors： Liao Z,Yuan C,Deng M,Li J,Chen J,Yang Y,Hu W,Liang S

更新日期：2006-12-26 00:00:00

abstract：:We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer in its native state. When BDNF unfolded in 6 M guanidine hydrochloride is diluted 20-fold into phosphate-buffered saline, a partially folded intermediate is rapi...

journal_title：Biochemistry

authors： Philo JS,Rosenfeld R,Arakawa T,Wen J,Narhi LO

更新日期：1993-10-12 00:00:00

abstract：:Aureochromes have recently been shown to act as blue-light-regulated transcription factors in the stramenopile alga Vaucheria frigida. They comprise a light-, oxygen-, or voltage-sensitive (LOV) domain as a sensory module with flavin mononucleotide (FMN) as a chromophore and a basic region leucine zipper (bZIP) domain...

journal_title：Biochemistry

authors： Herman E,Sachse M,Kroth PG,Kottke T

更新日期：2013-05-07 00:00:00

abstract：:The intestinal fatty acid binding protein is composed of two beta-sheets surrounding a large interior cavity. There is a small helical domain associated with the portal for entry of the ligand into the cavity. Denaturation of the protein has been monitored in a residue-specific manner by collecting a series of two-dim...

journal_title：Biochemistry

authors： Hodsdon ME,Frieden C

更新日期：2001-01-23 00:00:00

abstract：:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...

journal_title：Biochemistry

authors： Prevelige PE Jr,Fasman GD

更新日期：1987-05-19 00:00:00

abstract：:A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...

journal_title：Biochemistry

authors： Jacobson GR,Takacs BJ,Rosenbusch JP

更新日期：1976-06-01 00:00:00

abstract：:The aminoacylation kinetics of 19 different variants of yeast tRNATyr with nucleotide substitutions in positions 33-35 were determined. Substitution of the conserved uridine-33 does not alter the rate of aminoacylation. However, substitution of the anticodon position 34 or position 35 reduces Km from 2- to 10-fold and...

journal_title：Biochemistry

authors： Bare LA,Uhlenbeck OC

更新日期：1986-09-23 00:00:00

abstract：:Inheritance of apoE4 is a strong risk factor for the development of late-onset sporadic Alzheimer's disease (AD). Several lines of evidence suggest that apoE4 binds to the Alzheimer Abeta protein and, under certain experimental conditions, promotes formation of beta-sheet structures and amyloid fibrils. Deposition of ...

journal_title：Biochemistry

authors： Poeggeler B,Miravalle L,Zagorski MG,Wisniewski T,Chyan YJ,Zhang Y,Shao H,Bryant-Thomas T,Vidal R,Frangione B,Ghiso J,Pappolla MA

更新日期：2001-12-11 00:00:00

abstract：:The mechanism of binding of the substrates Mg x ATP and ribose 5-phosphate as well as Mg2+ to the enzyme 5-phospho-D-ribosyl (alpha-1-diphosphate synthetase from Escherichia coli has been analyzed. By use of the competive inhibitors of ATP and ribose 5-phosphate binding, alpha,beta-methylene ATP and (+)-1-alpha,2-alph...

journal_title：Biochemistry

authors： Willemoës M,Hove-Jensen B

更新日期：1997-04-22 00:00:00

abstract：:We reacted uracil-containing M13mp2 DNA with N-hydroxy-2-aminofluorene to produce a template with N-(deoxyguanosin-8-yl)-2-aminofluorene adducts. This template was hybridized to a non-uracil-containing linear fragment from which the lac z complementing insert had been removed to produce a gapped substrate. DNA synthes...

journal_title：Biochemistry

authors： Sahm J,Turkington E,LaPointe D,Strauss B

更新日期：1989-04-04 00:00:00

abstract：:Human S100B(beta beta) is a small intracellular EF-hand calcium-binding protein that consists of two noncovalently associated 91-residue beta monomers. The three-dimensional structures of S100B reveal the dimer interface consists of four alpha-helices (I, I' and IV, IV') packed in an X-type bundle. In this study, guan...

journal_title：Biochemistry

authors： Ferguson PL,Shaw GS

更新日期：2002-03-19 00:00:00