Monoclonal antibodies specific for poly(dG) X poly(dC) and poly(dG) X poly(dm5C).

abstract：:Chemoenzymatic glycan remodeling by endoglycosidase-catalyzed deglycosylation and reglycosylation is emerging as an attractive approach for producing homogeneous glycoforms of antibodies, and the success of this approach depends on the discovery of efficient endoglycosidases and their glycosynthase mutants. We report ...

journal_title：Biochemistry

authors： Tong X,Li T,Li C,Wang LX

更新日期：2018-09-04 00:00:00

abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

journal_title：Biochemistry

authors： Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

更新日期：1997-11-18 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:Decarboxylation reactions from comparable thiamin diphosphate- and thiamin-derived adducts of p-(halomethyl)benzoylformic acids in enzymic and non-enzymic reactions, respectively, reveal critical distinctions in otherwise similar Breslow intermediates. The ratio of protonation to chloride elimination from the Breslow ...

journal_title：Biochemistry

authors： Bielecki M,Howe GW,Kluger R

更新日期：2019-08-27 00:00:00

abstract：:The interfacial interactions of cholesterol with sphingomyelins (SMs) containing various homogeneous acyl chains have been investigated by Langmuir film balance approaches. Low in-plane elasticity among the packed lipids was identified as an important physical feature of the cholesterol-sphingomyelin liquid-ordered ph...

journal_title：Biochemistry

authors： Li XM,Momsen MM,Smaby JM,Brockman HL,Brown RE

更新日期：2001-05-22 00:00:00

abstract：:The circulating protein transthyretin (TTR) can unfold, oligomerize, and form highly structured amyloid fibrils that are deposited in tissues, causing organ damage and disease. This pathogenic process is caused by a heritable TTR point mutation in cases of familial TTR-related amyloidosis or wild-type TTR in cases of ...

journal_title：Biochemistry

authors： Greene MJ,Klimtchuk ES,Seldin DC,Berk JL,Connors LH

更新日期：2015-01-20 00:00:00

abstract：:Previous experiments have shown that a Synechocystis sp. PCC 6803 mutant (delta psbO) lacking the extrinsic manganese-stabilizing protein (MSP) exhibits impaired, but significant levels of H2O-splitting activity [Burnap, R., & Sherman, L.A. (1991) Biochemistry 30, 440-446]. [14C]DCMU-binding experiments now show that ...

journal_title：Biochemistry

authors： Burnap RL,Shen JR,Jursinic PA,Inoue Y,Sherman LA

更新日期：1992-08-18 00:00:00

abstract：:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...

journal_title：Biochemistry

authors： Lu J,Zhao L,Xia A,Xia T,Qi X

更新日期：2013-03-12 00:00:00

abstract：:Clostridium perfringens epsilon toxin belongs to the aerolysin-like family of pore-forming toxins and is one of the most potent bacterial toxins known. The epsilon toxin causes fatal enterotoxemia in sheep, goats, and possibly humans. Evidence indicates that the toxin binds to protein receptors including hepatitis A v...

journal_title：Biochemistry

authors： Ivie SE,McClain MS

更新日期：2012-09-25 00:00:00

abstract：:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...

journal_title：Biochemistry

authors： Rockwell P,Krakow JS

更新日期：1988-05-03 00:00:00

abstract：:Ca(2+)/calmodulin-dependent protein kinase kinases (CaMKKs) phosphorylate and activate specific downstream protein kinases, including CaMKI, CaMKIV, and 5'-AMP-activated protein kinase, which mediates a variety of Ca(2+) signaling cascades. CaMKKs have been shown to undergo autophosphorylation, although their role in ...

journal_title：Biochemistry

authors： Tokumitsu H,Hatano N,Fujimoto T,Yurimoto S,Kobayashi R

更新日期：2011-09-27 00:00:00

abstract：:The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...

journal_title：Biochemistry

authors： Kempton JB,Withers SG

更新日期：1992-10-20 00:00:00

abstract：:NAD(P)H:quinone oxidoreductase 1 (NQO1) is currently an emerging target in pancreatic cancer. In this report, we describe a series of indolequinones, based on 5-methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione (ES936), and evaluate NQO1 inhibition and growth inhibitory activity in the human pancreatic M...

journal_title：Biochemistry

authors： Reigan P,Colucci MA,Siegel D,Chilloux A,Moody CJ,Ross D

更新日期：2007-05-22 00:00:00

abstract：:Structures of glycopeptides obtained by exhaustive Pronase digestion of high molecular weight (1.7 X 10(5)) salmon egg polysialoglycoprotein have been elucidated. Six principal glycopeptides isolated by gel chromatography and DEAE-Sephadex A-25 chromatography in the absence or presence of borate ion were analyzed for ...

journal_title：Biochemistry

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1985-09-24 00:00:00

abstract：:Pyrimidine oligonucleotides have been shown to prime the E. coli DNA polymerase I repair reaction, specifically and reproducibly. DNA molecules up to 30 nucleotides long have been obtained from the extension of oligopyrimidine primers, 9 to 11 nucleotides long isolated from the complementary (minus) strand of bacterio...

journal_title：Biochemistry

authors： Kaptein JS,Spencer JH

更新日期：1978-03-07 00:00:00

abstract：:The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...

journal_title：Biochemistry

authors： Vertessy BG,Harmat V,Böcskei Z,Náray-Szabó G,Orosz F,Ovádi J

更新日期：1998-11-03 00:00:00

abstract：:The positions of the several sea urchin histone genes on the eukaryotic fragments of the chimeric plasmids pSp2 and pSp17 have been mapped relative to the Eco RI and Hind III restriction endonuclease sites on the plasmids. Two principal mapping methods using the electron microscope have been used: (a) the R-loop proce...

journal_title：Biochemistry

authors： Holmes DS,Cohn RH,Kedes LH,Davidson N

更新日期：1977-04-05 00:00:00

abstract：:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(II...

journal_title：Biochemistry

authors： Rich AM,Ellis PJ,Tennant L,Wright PE,Armstrong RS,Lay PA

更新日期：1999-12-14 00:00:00

abstract：:The proteins which have been left tightly bound to the tissue culture substrate after ethylenebis (oxyethyl-enenitrilo) tetraacetic acid (EGTA)-mediated removal of normal, virus-transformed, and revertant mouse cells and which have been implicated in the substrate adhesion process have been analyzed by slab sodium dod...

journal_title：Biochemistry

authors： Culp LA

更新日期：1976-09-07 00:00:00

abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...

journal_title：Biochemistry

authors： Haapalainen AM,Meriläinen G,Pirilä PL,Kondo N,Fukao T,Wierenga RK

更新日期：2007-04-10 00:00:00

abstract：:Thermobifida fusca Cel9A-90, an unusual family 9 enzyme, is a processive endoglucanase containing a catalytic domain closely linked to a family 3c cellulose binding domain (Cel9A-68) followed by a fibronectin III-like domain and a family 2 cellulose binding domain. To study its catalytic mechanism, 12 mutant genes wit...

journal_title：Biochemistry

authors： Zhou W,Irwin DC,Escovar-Kousen J,Wilson DB

更新日期：2004-08-03 00:00:00

abstract：:Cells can be transiently permeabilized by application of high electric pulses of short duration. A direct consequence of this treatment is to induce a fusogenic state in the pulsed membrane. The molecular events underlying these phenomena remain to be explained. During our work, we investigated the effects of pulsing ...

journal_title：Biochemistry

authors： Rols MP,Teissié J

更新日期：1990-05-15 00:00:00

abstract：:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...

journal_title：Biochemistry

authors： Varticovski L,Chahwala SB,Whitman M,Cantley L,Schindler D,Chow EP,Sinclair LK,Pepinsky RB

更新日期：1988-05-17 00:00:00

abstract：:The effect of concentrated glycerol on the thermal transitions of chymotrypsinogen and ribonuclease has been examined by differential spectrophotometry at 293 and 287 mm, respectively. It was found that for both proteins addition of glycerol raises the transition temperature, the increase in Tm being greater for ribon...

journal_title：Biochemistry

authors： Gekko K,Timasheff SN

更新日期：1981-08-04 00:00:00

abstract：:A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state k...

journal_title：Biochemistry

authors： Dooijewaard G,Roossien FF,Robillard GT

更新日期：1979-07-10 00:00:00

abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

journal_title：Biochemistry

authors： Westrick JA,Peters KS,Ropp JD,Sligar SG

更新日期：1990-07-17 00:00:00

abstract：:Native ribonucleotide reductase from Escherichia coli exhibits a resonance-enhanced Raman mode at 1498 cm-1 that is characteristic of a tyrosyl radical. The Raman frequency as well as the absorption maximum at 410 nm identifies the radical as being in a deprotonated state. The B2 subunit of ribonucleotide reductase sh...

journal_title：Biochemistry

authors： Backes G,Sahlin M,Sjöberg BM,Loehr TM,Sanders-Loehr J

更新日期：1989-02-21 00:00:00

abstract：:In the absence of any external electron donor, the "peroxy" intermediate of cytochrome c oxidase (CcO-607) is converted to the ferryl form (CcO-580) and subsequently to oxidized enzyme. The rate of conversion of CcO-607 to the CcO-580 form is pH dependent between pH 3.0 and pH 7.6. A plot of the logarithm of the rate ...

journal_title：Biochemistry

authors： Fabian M,Palmer G

更新日期：2001-02-13 00:00:00

abstract：:Time-resolved infrared (TRIR) techniques have been employed to study the reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome c oxidase (CcO). The ligation dynamics immediately following photodissociation have been investigated using picosecond TRIR spectroscopy and linear dichroism. The r...

journal_title：Biochemistry

authors： Dyer RB,Peterson KA,Stoutland PO,Woodruff WH

更新日期：1994-01-18 00:00:00

abstract：:There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...

journal_title：Biochemistry

authors： Danielson TA,Stine JM,Dar TA,Briknarova K,Bowler BE

更新日期：2017-12-26 00:00:00