当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Effects of an anti-beta monoclonal antibody on the interaction of the Escherichia coli RNA polymerase with the lac and TAC promoters.

Effects of an anti-beta monoclonal antibody on the interaction of the Escherichia coli RNA polymerase with the lac and TAC promoters.

Abstract：

:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templates of the lac and TAC16 promoters showed that abortive synthesis by mAb 210E8-RNA polymerase varied as a function of DNA topology. A kinetic analysis of RPl formation on the supercoiled lac UV5 promoter showed that mAb 210E8 effected a slight alteration in the isomerization rate and no effect on the initial rate of RNA polymerase binding to the promoter. The potent inhibition of initiation with linear promoters by mAb 210E8 was not apparent when the promoters were assayed in their supercoiled forms. Abortive synthesis with the TAC16 promoter was accompanied by an mAb 210E8 induced hindrance of ApUpU but not UpGpU synthesis. The data indicate that the inhibition by mAb 210E8 with the supercoiled TAC16 promoter is further alleviated when the spacer length is shifted from 16 base pairs (ApUpU formation) to 18 base pairs (UpGpU formation). When DNase I and dimethyl sulfate were used to probe DNA structure, mAb 210E8 was found to alter polymerase interactions with the lac promoter. DNase I footprinting indicated that the structural interactions for lac P+ promoter-RNA polymerase complexes were slightly altered in the presence of mAb 210E8. Treatment of the RNA polymerase-lac UV5 complex with dimethyl sulfate revealed an alternate mode of RNA polymerase interaction with essential guanine contacts which was intermediate between a fully protected and free promoter.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Rockwell P,Krakow JS

doi

10.1021/bi00409a058

subject

Has Abstract

pub_date

1988-05-03 00:00:00

pages

3512-20

issue

9

eissn

0006-2960

issn

1520-4995

journal_volume

27

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • pH-Dependent conformational stability of the ribotoxin alpha-sarcin and four active site charge substitution variants.

    abstract：:Alpha-sarcin is an exquisitely specific ribonuclease that binds and cleaves a single phosphodiester bond in the large rRNA of the eukaryotic ribosome, inactivating it. To better understand this remarkable activity, the contributions of the active site residues (His 50, Glu 96, and His 137) to the conformational stabil...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061273v

    authors： García-Mayoral MF,del Pozo AM,Campos-Olivas R,Gavilanes JG,Santoro J,Rico M,Laurents DV,Bruix M

    更新日期：2006-11-21 00:00:00

  • Function of the MexB efflux-transporter divided into two halves.

    abstract：:The MexA-MexB-OprM efflux pump exports structurally and functionally diverse xenobiotics and confers multi-drug resistance on Pseudomonas aeruginosa cells. The MexB transporter traverses the inner membrane twelve times, bears two large periplasmic domains and has two homologous tandem repeats. To test whether two homo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0300074

    authors： Eda S,Yoneyama H,Nakae T

    更新日期：2003-06-17 00:00:00

  • Characterization and purification of the monoamine transporter of bovine chromaffin granules.

    abstract：:The monoamine transporter of the chromaffin granule membranes can be specifically labeled by the photoaffinity reagent 7-azido-8-[125I]iodoketanserin. The characteristics of the labeled protein have been investigated. Two-dimensional gel electrophoresis of the labeled membranes indicated a MW of about 70,000 and an is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00122a012

    authors： Isambert MF,Gasnier B,Botton D,Henry JP

    更新日期：1992-02-25 00:00:00

  • Solution structures of spinach acyl carrier protein with decanoate and stearate.

    abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052062d

    authors： Zornetzer GA,Fox BG,Markley JL

    更新日期：2006-04-25 00:00:00

  • Stabilization of the red semiquinone form of pig kidney general acyl-CoA dehydrogenase by acyl coenzyme A derivatives.

    abstract：:Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00520a024

    authors： Mizzer JP,Thorpe C

    更新日期：1981-08-18 00:00:00

  • A β-Turn Motif in the Steroid Hormone Receptor's Ligand-Binding Domains Interacts with the Peptidyl-prolyl Isomerase (PPIase) Catalytic Site of the Immunophilin FKBP52.

    abstract：:The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor α (hERα) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II β-turn conformat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00506

    authors： Byrne C,Henen MA,Belnou M,Cantrelle FX,Kamah A,Qi H,Giustiniani J,Chambraud B,Baulieu EE,Lippens G,Landrieu I,Jacquot Y

    更新日期：2016-09-27 00:00:00

  • Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase.

    abstract：:Biotin synthase catalyzes the conversion of dethiobiotin (DTB) to biotin through the oxidative addition of sulfur between two saturated carbon atoms, generating a thiophane ring fused to the existing ureido ring. Biotin synthase is a member of the radical SAM superfamily, composed of enzymes that reductively cleave S-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201042r

    authors： Taylor AM,Stoll S,Britt RD,Jarrett JT

    更新日期：2011-09-20 00:00:00

  • Phenotypic regulation of the sphingosine 1-phosphate receptor miles apart by G protein-coupled receptor kinase 2.

    abstract：:The evolutionarily conserved DRY motif at the end of the third helix of rhodopsin-like, class-A G protein-coupled receptors (GPCRs) is a major regulator of receptor stability, signaling activity, and β-arrestin-mediated internalization. Substitution of the DRY arginine with histidine in the human vasopressin receptor ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi501061h

    authors： Burczyk M,Burkhalter MD,Blätte T,Matysik S,Caron MG,Barak LS,Philipp M

    更新日期：2015-01-27 00:00:00

  • Rational engineering of mannosyl binding in the distal glycone subsites of Cellulomonas fimi endo-beta-1,4-mannanase: mannosyl binding promoted at subsite -2 and demoted at subsite -3.

    abstract：:To date, rational redesign of glycosidase active-site clefts has been mainly limited to the removal of essential functionalities rather than their introduction. The glycoside hydrolase family 26 endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi depolymerizes various abundant plant mannans. On the basis...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100097f

    authors： Hekmat O,Lo Leggio L,Rosengren A,Kamarauskaite J,Kolenova K,Stålbrand H

    更新日期：2010-06-15 00:00:00

  • Interfacial properties of the polyheme cytochrome c3 superfamily from Desulfovibrio.

    abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00036a005

    authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

    更新日期：1995-09-12 00:00:00

  • Nucleosome rearrangement in human cells following short patch repair of DNA damaged by bleomycin.

    abstract：:We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00484a020

    authors： Sidik K,Smerdon MJ

    更新日期：1990-08-14 00:00:00

  • Nearest neighbor analysis of the SecYEG complex. 2. Identification of a SecY-SecE cytosolic interface.

    abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034333v

    authors： Satoh Y,Mori H,Ito K

    更新日期：2003-06-24 00:00:00

  • Escherichia coli aromatic amino acid aminotransferase: characterization and comparison with aspartate aminotransferase.

    abstract：:Aromatic amino acid aminotransferase (ArAT) from Escherichia coli was overexpressed in E. coli cells, purified, and characterized. The enzyme was similar to aspartate aminotransferase (AspAT) of E. coli in many aspects, such as gross protein structure and spectroscopic properties. The reactions of pyridoxal 5'-phospha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00096a036

    authors： Hayashi H,Inoue K,Nagata T,Kuramitsu S,Kagamiyama H

    更新日期：1993-11-16 00:00:00

  • Kinetic consequences of introducing a proximal selenocysteine ligand into cytochrome P450cam.

    abstract：:The structural, electronic, and catalytic properties of cytochrome P450cam are subtly altered when the cysteine that coordinates to the heme iron is replaced with a selenocysteine. To map the effects of the sulfur-to-selenium substitution on the individual steps of the catalytic cycle, we conducted a comparative kinet...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00939

    authors： Vandemeulebroucke A,Aldag C,Stiebritz MT,Reiher M,Hilvert D

    更新日期：2015-11-10 00:00:00

  • Purification and characterization of CTP synthetase, the product of the URA7 gene in Saccharomyces cerevisiae.

    abstract：:In the yeast Saccharomyces cerevisiae, CTP synthetase [EC 6.3.4.2; UTP:ammonia ligase (ADP-forming)] is the product of the URA7 gene. CTP synthetase was purified 503-fold to apparent homogeneity from cells bearing the URA7 gene on a multicopy plasmid that directed a 10-fold overproduction of the enzyme. The purificati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00201a028

    authors： Yang WL,McDonough VM,Ozier-Kalogeropoulos O,Adeline MT,Flocco MT,Carman GM

    更新日期：1994-09-06 00:00:00

  • Binding of copper and silver to single-site variants of peptidylglycine monooxygenase reveals the structure and chemistry of the individual metal centers.

    abstract：:Peptidylglycine monooxygenase (PHM) catalyzes the final step in the biosynthesis of amidated peptides that serve as important signaling molecules in numerous endocrine pathways. The catalytic mechanism has attracted much attention because of a number of unique attributes, including the presence of a pair of uncoupled ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4015264

    authors： Chauhan S,Kline CD,Mayfield M,Blackburn NJ

    更新日期：2014-02-18 00:00:00

  • Effect of basic compounds on the polymerization of clathrin.

    abstract：:The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00526a028

    authors： Nandi PK,Van Jaarsveld PP,Lippoldt RE,Edelhoch H

    更新日期：1981-11-10 00:00:00

  • The amino- and carboxyl-terminal fragments of the Bacillus thuringensis Cyt1Aa toxin have differential roles in toxin oligomerization and pore formation.

    abstract：:The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101239r

    authors： Rodriguez-Almazan C,Ruiz de Escudero I,Cantón PE,Muñoz-Garay C,Pérez C,Gill SS,Soberón M,Bravo A

    更新日期：2011-01-25 00:00:00

  • Membrane topography of the photosynthetic reaction center polypeptides of Rhodopseudomonas sphaeroides.

    abstract：:The topography of the photosynthetic reaction center (RC) polypeptides (H, M, and L) was investigated by proteolysis and radioiodination of membrane vesicles isolated from Rhodopseudomonas sphaeroides. Chromatophores, obtained from French-pressed cell lysates, are closed vesicles' and oriented inside out with respect ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00519a012

    authors： Bachmann RC,Gillies K,Takemoto JY

    更新日期：1981-08-04 00:00:00

  • cAMP binds with high affinity and specificity to the androgen receptor from murine skeletal muscle.

    abstract：:cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00423a013

    authors： Ofenloch-Hähnle B,Eisele K

    更新日期：1988-11-15 00:00:00

  • N-terminal region of CusB is sufficient for metal binding and metal transfer with the metallochaperone CusF.

    abstract：:Gram-negative bacteria, such as Escherichia coli, utilize efflux resistance systems in order to expel toxins from their cells. Heavy-metal resistance is mediated by resistance nodulation cell division (RND)-based efflux pumps composed of a tripartite complex that includes an RND-transporter, an outer-membrane factor (...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300596a

    authors： Mealman TD,Zhou M,Affandi T,Chacón KN,Aranguren ME,Blackburn NJ,Wysocki VH,McEvoy MM

    更新日期：2012-08-28 00:00:00

  • NMR solution structure of a synthetic troponin C heterodimeric domain.

    abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9528006

    authors： Shaw GS,Sykes BD

    更新日期：1996-06-11 00:00:00

  • Carbon-13 nuclear magnetic resonance studies on the interaction of glycophorin with lecithin in reconstituted vesicles.

    abstract：:Glycophorin, the MN blood group substance, is a major intrinsic glycoprotein in erythrocyte membranes. The interaction of glycophorin with phosphatidylcholine, 13C-labeled in specific positions in reconstituted unilamellar vesicles, was investigated by using the 13C NMR technique. 1-Palmitoyl-2-([14-13C]linoleoyl)-sn-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00552a016

    authors： Utsumi H,Tunggal BD,Stoffel W

    更新日期：1980-05-27 00:00:00

  • Acid- and calcium-induced structural changes in phosphatidylethanolamine membranes stabilized by cholesteryl hemisuccinate.

    abstract：:The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00328a013

    authors： Lai MZ,Vail WJ,Szoka FC

    更新日期：1985-03-26 00:00:00

  • Primary structure of Escherichia coli ribosomal protein L31.

    abstract：:Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniq...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00596a020

    authors： Brosius J

    更新日期：1978-02-07 00:00:00

  • Purification and biochemical characterization of tubulin from the budding yeast Saccharomyces cerevisiae.

    abstract：:We describe a method for isolating milligram quantities of assembly-competent tubulin from the budding yeast Saccharomyces cerevisiae. The tubulin is > 95% purified and free of contaminating enzyme activities. As a result, it has been possible to determine the yeast tubulin equilibrium-binding constant for Mg-GTP and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00085a013

    authors： Davis A,Sage CR,Wilson L,Farrell KW

    更新日期：1993-08-31 00:00:00

  • Subtle Dynamic Changes Accompany Hck Activation by HIV-1 Nef and are Reversed by an Antiretroviral Kinase Inhibitor.

    abstract：:The HIV-1 virulence factor Nef interacts with the macrophage Src-family kinase Hck, resulting in constitutive kinase activation that contributes to viral replication and immune escape. Previous chemical library screens identified the diphenylfuranopyrimdine kinase inhibitor DFP-4AB, which selectively inhibits Nef-depe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00875

    authors： Wales TE,Hochrein JM,Morgan CR,Emert-Sedlak LA,Smithgall TE,Engen JR

    更新日期：2015-10-20 00:00:00

  • Guanosine tetraphyosphate and its analogues. Chemical synthesis of guanosine 3',5'-dipyrophosphate, deoxyguanosine 3',5'-dipyrophosphate, guanosine 2',5'-bis(methylenediphosphonate), and guanosine 3',5'-bis(methylenediphosphonate).

    abstract：:A new procedure for the synthesis of the pyrophosphate bond has been employed in the preparation of nucleoside dipyrophosphates from nucleoside 3',5'-diphosphates. The method makes use of a powerful phosphorylating agent generated in a mixture of cyanoethyl phosphate, dicyclohexylcarbodiimide, and mesitylenesulfonyl c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00666a012

    authors： Bennett GN,Gough GR,Gilham PT

    更新日期：1976-10-19 00:00:00

  • Carbon monoxide binding to human hemoglobin A0.

    abstract：:The carbon monoxide binding curve to human hemoglobin A0 has been measured to high precision in experimental conditions of 600 microM heme, 0.1 M N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, 0.1 M NaCl, 10 mM inositol hexaphosphate, 1 mM disodium ethylenediaminetetraacetic acid, pH 6.94, and 25 degrees C. Co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00394a031

    authors： Di Cera E,Doyle ML,Connelly PR,Gill SJ

    更新日期：1987-10-06 00:00:00

  • 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract.

    abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062117r

    authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

    更新日期：2007-06-05 00:00:00