Subtle Dynamic Changes Accompany Hck Activation by HIV-1 Nef and are Reversed by an Antiretroviral Kinase Inhibitor.

abstract：:Colicin E3 is a 60 kDa, multidomain protein antibiotic that targets its ribonuclease activity to an essential region of the 16S ribosomal RNA of Escherichia coli. To prevent suicide of the producing cell, synthesis of the toxin is accompanied by the production of a 10 kDa immunity protein (Im3) that binds strongly to ...

journal_title：Biochemistry

authors： Walker D,Moore GR,James R,Kleanthous C

更新日期：2003-04-15 00:00:00

abstract：:Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...

journal_title：Biochemistry

authors： Marletta MA,Yoon PS,Iyengar R,Leaf CD,Wishnok JS

更新日期：1988-11-29 00:00:00

abstract：:Mouse photoreceptor function and survival critically depend on Ca(2+)-regulated retinal membrane guanylyl cyclase (RetGC), comprised of two isozymes, RetGC1 and RetGC2. We characterized the content, catalytic constants, and regulation of native RetGC1 and RetGC2 isozymes using mice lacking guanylyl cyclase activating ...

journal_title：Biochemistry

authors： Peshenko IV,Olshevskaya EV,Savchenko AB,Karan S,Palczewski K,Baehr W,Dizhoor AM

更新日期：2011-06-28 00:00:00

abstract：:The properties of a series of new potentiometric membrane probes have been explored. The probes all contain an (aminostyryl)pyridinium chromophore or a more highly conjugated analogue. The spectral properties of the dyes are discussed in terms of the excitation-induced charge shift from the pyridine to the aniline; th...

journal_title：Biochemistry

authors： Fluhler E,Burnham VG,Loew LM

更新日期：1985-10-08 00:00:00

abstract：:Synthesis of a novel perylene-neomycin conjugate (3) and the properties of its binding to human telomeric G-quadruplex DNA, 5'-d[AG3(T2AG3)3] (4), are reported. Various spectroscopic techniques were employed to characterize the binding of conjugate 3 to 4. A competition dialysis assay revealed that 3 preferentially bi...

journal_title：Biochemistry

authors： Xue L,Ranjan N,Arya DP

更新日期：2011-04-12 00:00:00

abstract：:In the anaerobic sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough (DvH), the genome sequencing revealed the presence of three operons encoding formate dehydrogenases. fdh1 encodes an alphabetagamma trimeric enzyme containing 11 heme binding sites; fdh2 corresponds to an alphabetagamma trimeric enzyme wi...

journal_title：Biochemistry

authors： ElAntak L,Dolla A,Durand MC,Bianco P,Guerlesquin F

更新日期：2005-11-15 00:00:00

abstract：:P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...

journal_title：Biochemistry

authors： Gayet L,Dayan G,Barakat S,Labialle S,Michaud M,Cogne S,Mazane A,Coleman AW,Rigal D,Baggetto LG

更新日期：2005-03-22 00:00:00

abstract：:Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...

journal_title：Biochemistry

authors： Pohjanjoki P,Lahti R,Goldman A,Cooperman BS

更新日期：1998-02-17 00:00:00

abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...

journal_title：Biochemistry

authors： Strokopytov B,Knegtel RM,Penninga D,Rozeboom HJ,Kalk KH,Dijkhuizen L,Dijkstra BW

更新日期：1996-04-02 00:00:00

abstract：:The primary photochemical event of photoactive yellow protein (PYP) was studied by laser flash photolysis experiments on a subpicosecond-nanosecond time scale. PYP was excited by a 390-nm pulse, and the transient difference absorption spectra were recorded by a multichannel spectrometer for a more reliable spectral an...

journal_title：Biochemistry

authors： Imamoto Y,Kataoka M,Tokunaga F,Asahi T,Masuhara H

更新日期：2001-05-22 00:00:00

abstract：:The Ca2+-dependent regulation of smooth muscle actomyosin involves a myosin light chain kinase (ATP: myosin light chain phosphotransferase). It has been shown (Dabrowska, R., Aromatorio, D., Sherry, J.M.F., and Hartshorne, D.J. 1977, Biochem. Biophys. Res. Commun. 78, 1263) that the kinase is composed of two proteins ...

journal_title：Biochemistry

authors： Dabrowska R,Sherry JM,Aromatorio DK,Hartshorne DJ

更新日期：1978-01-24 00:00:00

abstract：:Cholesterol and its hemisuccinate and sulfate derivatives are widely used in studies of purified membrane proteins but are difficult to solubilize in aqueous solution, even in the presence of detergent micelles. Other cholesterol derivatives do not form conventional micelles and lead to viscous solutions. To address t...

journal_title：Biochemistry

authors： Howell SC,Mittal R,Huang L,Travis B,Breyer RM,Sanders CR

更新日期：2010-11-09 00:00:00

abstract：:The photoaffinity reagent 6-azido-L-tryptophan was synthesized by chemical methods. It binds reversibly in the dark to the alpha 2 beta 2 complex of tryptophan synthase of Escherichia coli and forms a quinonoid intermediate with enzyme-bound pyridoxal phosphate (lambda max = 476 nm). The absorbance of this chromophore...

journal_title：Biochemistry

authors： Miles EW,Phillips RS

更新日期：1985-08-13 00:00:00

abstract：:Tissue plasminogen activator-inhibitor complexes were purified from the conditioned medium of human umbilical vein endothelial cells by affinity chromatography followed by gel filtration. It was found that a single complex was isolated which can exist in two distinct interconvertible conformations. These may be separa...

journal_title：Biochemistry

authors： Sanzo MA,Marasa JC,Wittwer AJ,Siegel NR,Harakas NK,Feder J

更新日期：1987-11-17 00:00:00

abstract：:The ability of DNA topoisomerase I to mediate the formation of structurally modified DNA duplexes was studied utilizing suicide substrates containing high-efficiency cleavage sites and acceptor oligonucleotides in which the 5'-terminal nucleotides were varied. When the substrates were nicked duplexes, the divalent cat...

journal_title：Biochemistry

authors： Wang X,Henningfeld KA,Hecht SM

更新日期：1998-02-24 00:00:00

abstract：:Single-molecule fluorescence resonance energy transfer (smFRET) methods were used to study the assembly pathway and DNA unwinding activity of the bacteriophage T4 helicase-primase (primosome) complex. The helicase substrates used were surface-immobilized model DNA replication forks "internally" labeled in the duplex r...

journal_title：Biochemistry

authors： Lee W,Jose D,Phelps C,Marcus AH,von Hippel PH

更新日期：2013-05-07 00:00:00

abstract：:A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...

journal_title：Biochemistry

authors： Llarrull LI,Mobashery S

更新日期：2012-06-12 00:00:00

abstract：:Cytochrome P450 (P450) 3A4 is the most abundant human P450 and oxidizes a diversity of substrates, including various drugs, steroids, carcinogens, and macrolide natural products. In some reactions, positive cooperativity has been reported in microsomal studies. Flavonoids, e.g., 7,8-benzoflavone (alpha-naphthoflavone,...

journal_title：Biochemistry

authors： Ueng YF,Kuwabara T,Chun YJ,Guengerich FP

更新日期：1997-01-14 00:00:00

abstract：:The dependence of the activity of recombinant activated human protein C (r-APC) on each of its nine gamma-carboxyglutamic (Gla) residues (sequence positions 6, 7, 14, 16, 19, 20, 25, 26, and 29) has been assessed in purified systems and in plasma using r-mutants in which each Gla residue of r-APC was individually alte...

journal_title：Biochemistry

authors： Jhingan A,Zhang L,Christiansen WT,Castellino FJ

更新日期：1994-02-22 00:00:00

abstract：:Human lecithin:cholesterol acyltransferase (LCAT, E.C.2.3.1.43) is a serine-type esterase that contains six cysteines, two of which, Cys31 and Cys184, are free. The remaining cysteines form disulfide links. One of these is between Cys50 and Cys74 and the other is between Cys313 and Cys356. The cDNA of LCAT and mutants...

journal_title：Biochemistry

authors： Qu SJ,Fan HZ,Blanco-Vaca F,Pownall HJ

更新日期：1993-03-30 00:00:00

abstract：:Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covalent complex containing ...

journal_title：Biochemistry

authors： Graves KL,Hardy LW

更新日期：1994-11-08 00:00:00

abstract：:Ammonia is an inhibitor of water oxidation and a structural analogue for substrate water, making it a valuable probe for the structural properties of the possible substrate-binding site on the oxygen-evolving complex (OEC) in photosystem II (PSII). By using the NH(3)-induced upshift of the 1365 cm(-)(1) IR mode in the...

journal_title：Biochemistry

authors： Fang CH,Chiang KA,Hung CH,Chang K,Ke SC,Chu HA

更新日期：2005-07-19 00:00:00

abstract：:The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on...

journal_title：Biochemistry

authors： Labuda D,Pörschke D

更新日期：1980-08-05 00:00:00

abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

journal_title：Biochemistry

authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

更新日期：1986-09-23 00:00:00

abstract：:We have found a correlation between liposome fusion kinetics and lipid phase behavior for several inverted phase forming lipids. N-Methylated dioleoylphosphatidylethanolamine (DOPE-Me), or mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC), will form an inverted hexagonal phase ...

journal_title：Biochemistry

authors： Ellens H,Siegel DP,Alford D,Yeagle PL,Boni L,Lis LJ,Quinn PJ,Bentz J

更新日期：1989-05-02 00:00:00

abstract：:The residue C221 on pyruvate decarboxylase (EC. 4.1.1.1) from Saccharomyces cerevisiae has been shown to be the site where the substrate activation cascade is triggered [Baburina et al. (1994) Biochemistry 33, 5630-5635] and is located on the beta domain [Arjunan et al. (1996) J. Mol. Biol. 256, 590], while the active...

journal_title：Biochemistry

authors： Baburina I,Dikdan G,Guo F,Tous GI,Root B,Jordan F

更新日期：1998-02-03 00:00:00

abstract：:The filamentous coliphage M13 possesses multiple copies of a 50-residue coat protein which is inserted into the inner membrane of Escherichia coli during infection. 13C nuclear magnetic resonance (NMR) spectroscopy has been used to probe the structure and dynamics of M13 coat protein solubilized in detergent micelles....

journal_title：Biochemistry

authors： Henry GD,Weiner JH,Sykes BD

更新日期：1986-02-11 00:00:00

abstract：:The late intermediates involved in the activation mechanism of bovine rhodopsin are investigated by time-resolved optical absorption spectroscopy. Measurements from 10 μs to 200 ms after photolysis were carried out on membrane suspensions of bovine rhodopsin at a temperature of 15 °C and at pH of 7.3, 8.0, and 8.7. Th...

journal_title：Biochemistry

authors： Szundi I,Funatogawa C,Kliger DS

更新日期：2016-09-13 00:00:00

abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...

journal_title：Biochemistry

authors： Laberge M,Osvath S,Fidy J

更新日期：2001-08-07 00:00:00

abstract：:Membrane-bound mammalian adenylyl cyclase isoforms contain two internally homologous cytoplasmic domains (C1 and C2). When expressed separately, C1 and C2 are catalytically inactive, but conversion of ATP to cAMP is observed if C1 and C2 are combined. By analogy with DNA polymerases, adenylyl cyclases are thought to r...

journal_title：Biochemistry

authors： Mitterauer T,Hohenegger M,Tang WJ,Nanoff C,Freissmuth M

更新日期：1998-11-17 00:00:00