Abstract:
:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns. The enthalpy of the intermediate increases by approximately 7 kcal/mol upon replacing arginine-45 with either asparagine or glycine. These observations support recent proposals that an arginine-45 salt bridge is broken upon ligand dissociation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Westrick JA,Peters KS,Ropp JD,Sligar SGdoi
10.1021/bi00480a026subject
Has Abstractpub_date
1990-07-17 00:00:00pages
6741-6issue
28eissn
0006-2960issn
1520-4995journal_volume
29pub_type
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