当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Determination of Fe-ligand bond lengths and the Fe-N-O bond angles in soybean ferrous and ferric nitrosylleghemoglobin a using multiple-scattering XAFS analyses.

Determination of Fe-ligand bond lengths and the Fe-N-O bond angles in soybean ferrous and ferric nitrosylleghemoglobin a using multiple-scattering XAFS analyses.

Abstract：

:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(III)NO at 10 K. The XANES and electronic spectra of the NO adducts are similar in shape and energies to the myoglobin (Mb) analogues. The environment of the Fe atom has been refined using multiple-scattering (MS) analyses of the XAFS data. For Lb(II)NO, the MS analysis resulted in an averaged Fe-N(p)(pyrrole) distance of 2.02 A, an Fe-N(epsilon)(imidazole) distance of 1.98 A, an Fe-N(NO) distance of 1.77 A, and an Fe-N-O angle of 147 degrees. The Fe-N(NO) distance and Fe-N-O angle obtained from the analysis of Lb(II)NO are in good agreement with those determined crystallographically for [Fe(TPP)(NO)] (TPP, tetraphenylporphyrinato), with and without 1-methylimidazole (1-MeIm) as the sixth ligand, and the MS XAFS structures reported previously for the myoglobin (Mb(II)NO) analogue and [Fe(TPP)(NO)]. The MS analysis of Lb(III)NO yielded an average Fe-N(p) distance of 2.00 A, an Fe-N(epsilon) distance of 1.89 A, an Fe-N(NO) distance of 1.68 A, and an Fe-N-O angle of 173 degrees. These bond lengths and angles are consistent with those determined previously for the myoglobin analogue (Mb(III)NO) and the crystal structures of the model complexes, [Fe(III)(TPP)(NO)(OH(2))](+) and [Fe(OEP)(NO)](+) (OEP, octaethylporphyrinato). The final XAFS R values were 16.1 and 18.2% for Lb(II)NO and Lb(III)NO, respectively.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Rich AM,Ellis PJ,Tennant L,Wright PE,Armstrong RS,Lay PA

doi

10.1021/bi990730n

subject

Has Abstract

pub_date

1999-12-14 00:00:00

pages

16491-9

issue

50

eissn

0006-2960

issn

1520-4995

pii

bi990730n

journal_volume

38

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Conformation of parathyroid hormone: time-resolved fluorescence studies.

    abstract：:Conformational and environmental changes in the functionally significant amino-terminal region of human parathyroid hormone (hPTH), induced by solvent or by complexation with acidic lipid, have been investigated. Structural perturbations were monitored by their effect on the fluorescence decay kinetics of the single t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00152a032

    authors： Willis KJ,Szabo AG

    更新日期：1992-09-22 00:00:00

  • Normal cellular Ha ras p21 protein causes local disruption of bilayer phospholipid.

    abstract：:We have investigated the interactions of the p21 protein of c-Ha ras with its phospholipid environment. Gel filtration of detergent-"solubilized" p21 revealed that this preparation consisted of a mixture of multimolecular aggregates of protein and phospholipid and also a population of individual p21 molecules. Additio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00412a028

    authors： Montgomery GW,Jagger BA,Bailey PD

    更新日期：1988-06-14 00:00:00

  • Human and murine cytotoxic T lymphocyte serine proteases: subsite mapping with peptide thioester substrates and inhibition of enzyme activity and cytolysis by isocoumarins.

    abstract：:The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00222a027

    authors： Odake S,Kam CM,Narasimhan L,Poe M,Blake JT,Krahenbuhl O,Tschopp J,Powers JC

    更新日期：1991-02-26 00:00:00

  • Alternatively spliced form of human thyroperoxidase, TPOzanelli: activity, intracellular trafficking, and role in hormonogenesis.

    abstract：:Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0026324

    authors： Niccoli-Sire P,Fayadat L,Siffroi-Fernandez S,Malthierry Y,Franc JL

    更新日期：2001-02-27 00:00:00

  • Solution structure of a band 3 peptide inhibitor bound to aldolase: a proposed mechanism for regulating binding by tyrosine phosphorylation.

    abstract：:Human erythrocyte band 3 inhibits glycolytic enzymes, including aldolase, by binding these cytoplasmic enzymes at its N-terminus. Phosphorylation of Y8 disrupts inhibition, and there is evidence that in vivo glycolysis levels in erythrocytes are regulated in part by a phosphorylation/dephosphorylation signaling pathwa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00051a005

    authors： Schneider ML,Post CB

    更新日期：1995-12-26 00:00:00

  • Interactions of factor XIII with fibrin as substrate and cofactor.

    abstract：:Factor XIIIa (a2') is a homodimeric transglutaminase that is formed via limited alpha-thrombin-catalyzed proteolysis of the platelet (a2) or plasma (a2b2) factor XIII zymogen in a reaction that results in proteolytic removal of a 37-aminoacyl residue peptide from the N-terminus of the a chains and exposure of the acti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00117a017

    authors： Hornyak TJ,Shafer JA

    更新日期：1992-01-21 00:00:00

  • Stable substructures of eightfold beta alpha-barrel proteins: fragment complementation of phosphoribosylanthranilate isomerase.

    abstract：:The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00129a010

    authors： Eder J,Kirschner K

    更新日期：1992-04-14 00:00:00

  • Role of the linker region connecting the reductase and heme domains in cytochrome P450BM-3.

    abstract：:Cytochrome P450BM-3 is a fatty acid monooxygenase that contains the catalytic P450 heme domain covalently attached to a diflavin P450 reductase domain. The function of the linker region connecting the C-terminal end of the heme domain to the N-terminal end of the reductase domain has been studied by deleting parts of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00035a031

    authors： Govindaraj S,Poulos TL

    更新日期：1995-09-05 00:00:00

  • The Q-loop of DrrA is involved in producing the closed conformation of the nucleotide binding domains and in transduction of conformational changes between DrrA and DrrB.

    abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701699a

    authors： Rao DK,Kaur P

    更新日期：2008-03-04 00:00:00

  • Base protonation facilitates B-Z interconversions of poly(dG-dC) X poly(dG-dC).

    abstract：:Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00320a041

    authors： Chen FM

    更新日期：1984-12-04 00:00:00

  • Direct spectroscopic and kinetic evidence for the involvement of a peroxodiferric intermediate during the ferroxidase reaction in fast ferritin mineralization.

    abstract：:Rapid freeze-quench (RFQ) Mössbauer and stopped-flow absorption spectroscopy were used to monitor the ferritin ferroxidase reaction using recombinant (apo) frog M ferritin; the initial transient ferric species could be trapped by the RFQ method using low iron loading (36 Fe2+/ferritin molecule). Biphasic kinetics of f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980847w

    authors： Pereira AS,Small W,Krebs C,Tavares P,Edmondson DE,Theil EC,Huynh BH

    更新日期：1998-07-14 00:00:00

  • An attempt to discriminate catalytic and regulatory proton binding sites in membrane-bound, thiol-reduced chloroplast ATPase.

    abstract：:The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00132a013

    authors： Valerio M,de Kouchkovsky Y,Haraux F

    更新日期：1992-05-05 00:00:00

  • Mechanism of membrane permeabilization by sticholysin I, a cytolysin isolated from the venom of the sea anemone Stichodactyla helianthus.

    abstract：:Actinaria cytolysins are very potent basic toxins isolated from the venom of sea anemones, which are supposed to exert their toxic activity through formation of oligomeric pores in the host plasma membrane. To gain insight into their mechanism of action, the interaction of Stichodactyla helianthus sticholysin I (St-I)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960787z

    authors： Tejuca M,Serra MD,Ferreras M,Lanio ME,Menestrina G

    更新日期：1996-11-26 00:00:00

  • Location of major antigenic sites of the beta subunit of human chorionic gonadotropin.

    abstract：:Purified beta subunit of human chorionic gonadotropin (hCG) was partially reduced with beta-mercaptoethanol, carboxymethylated, and digested with chymotrypsin. The peptides were isolated by high-voltage electrophoresis and paper chromatography. Five major disulfide-containing peptides were isolated, and their location...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00619a034

    authors： Swaminathan N,Braunstein GD

    更新日期：1978-12-26 00:00:00

  • Engineering of papain: selective alteration of substrate specificity by site-directed mutagenesis.

    abstract：:The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00101a003

    authors： Khouri HE,Vernet T,Ménard R,Parlati F,Laflamme P,Tessier DC,Gour-Salin B,Thomas DY,Storer AC

    更新日期：1991-09-17 00:00:00

  • Contributions of asparagine at alpha 97 to the cooperative oxygenation process of hemoglobin.

    abstract：:According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952518z

    authors： Kim HW,Shen TJ,Ho NT,Zou M,Tam MF,Ho C

    更新日期：1996-05-28 00:00:00

  • Decay of activated Bacillus subtilis pho response regulator, PhoP approximately P, involves the PhoR approximately P intermediate.

    abstract：:PhoR of Bacillus subtilis is a histidine sensor-kinase belonging to the family of two-component signal transduction systems. PhoR is responsible for processing the phosphate-starvation signal and providing phosphate input to regulate the level of phosphorylated response regulator, PhoP, which activates/represses Pho r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990658t

    authors： Shi L,Liu W,Hulett FM

    更新日期：1999-08-03 00:00:00

  • Evidence that eosin-5-maleimide binds close to the anion transport site of human erythrocyte band 3: a fluorescence quenching study.

    abstract：:The interaction between eosin-5-maleimide (EMA), an inhibitor of the anion transport protein, band 3, and I-, a transportable substrate, was investigated by fluorescence quenching. The Stern-Volmer plot for the quenching reaction between EMA-labeled band 3 and I- exhibits downward curvature both in human erythrocyte g...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00014a045

    authors： Pan RJ,Cherry RJ

    更新日期：1995-04-11 00:00:00

  • Mechanism of bypass synthesis through an abasic site analog by DNA polymerase I.

    abstract：:Bypass synthesis by DNA polymerase I was studied using synthetic 40-nucleotide-long gapped duplex DNAs each containing a site-specific abasic site analog, as a model system for mutagenesis associated with DNA lesions. Bypass synthesis proceeded in two general stages: a fast polymerization stage that terminated opposit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9621324

    authors： Paz-Elizur T,Takeshita M,Livneh Z

    更新日期：1997-02-18 00:00:00

  • Kinetics of the interaction of 2'(3')-O-(N-methylanthraniloyl)-ATP with myosin subfragment 1 and actomyosin subfragment 1: characterization of two acto-S1-ADP complexes.

    abstract：:We have used a fluorescent analogue of ATP, mantATP [2'(3')-O-(N-methylanthraniloyl)-adenosine 5'-triphosphate; Hiratsuka T. (1983) Biochim. Biophys. Acta 742, 496-508], and made a detailed kinetic study of the interaction of mantATP and mantADP with S1 and acto-S1. We have shown that these analogues behave like ATP a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00216a017

    authors： Woodward SK,Eccleston JF,Geeves MA

    更新日期：1991-01-15 00:00:00

  • Phosphate inhibition of the copper- and zinc-containing superoxide dismutase: a reexamination.

    abstract：:Phosphate was reported to be an inhibitor of copper- and zinc-containing superoxide dismutase (SOD) [de Freitas, D.M., & Valentine, J.S. (1984) Biochemistry 23, 2079-2082]. Thus SOD activity, in 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (pH 7.4), was decreased by approximately 50% when the assay...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a037

    authors： Beyer WF Jr,Wang Y,Fridovich I

    更新日期：1986-10-07 00:00:00

  • The C-terminal lipid-binding domain of apolipoprotein E is a highly efficient mediator of ABCA1-dependent cholesterol efflux that promotes the assembly of high-density lipoproteins.

    abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi602407r

    authors： Vedhachalam C,Narayanaswami V,Neto N,Forte TM,Phillips MC,Lund-Katz S,Bielicki JK

    更新日期：2007-03-13 00:00:00

  • Identification of basic amino acid residues important for citrate binding by the periplasmic receptor domain of the sensor kinase CitA.

    abstract：:The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0340595

    authors： Gerharz T,Reinelt S,Kaspar S,Scapozza L,Bott M

    更新日期：2003-05-20 00:00:00

  • Product dependence of deuterium isotope effects in enzyme-catalyzed reactions.

    abstract：:Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00058a013

    authors： Cook PF,Yoon MY,Hara S,McClure GD Jr

    更新日期：1993-02-23 00:00:00

  • Kinetic and Spectroscopic Characterization of the Catalytic Ternary Complex of Tryptophan 2,3-Dioxygenase.

    abstract：:The first step of the kynurenine pathway for l-tryptophan (l-Trp) degradation is catalyzed by heme-dependent dioxygenases, tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase. In this work, we employed stopped-flow optical absorption spectroscopy to study the kinetic behavior of the Michaelis complex of C...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00179

    authors： Geng J,Weitz AC,Dornevil K,Hendrich MP,Liu A

    更新日期：2020-08-04 00:00:00

  • Mechanistic diversity in a metalloenzyme superfamily.

    abstract：:It is now appreciated that the relationships of proteins, particularly enzymes, within a protein superfamily can be understood not only in terms of their sequence similarities and three-dimensional structures but also by chemical threads that relate their functional attributes. The mechanistic ties among superfamily m...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi001814v

    authors： Armstrong RN

    更新日期：2000-11-14 00:00:00

  • Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site.

    abstract：:Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00271a036

    authors： Dalbey RE,Wells JA,Yount RG

    更新日期：1983-01-18 00:00:00

  • Gel to liquid-crystalline transition temperatures of water dispersions of two pairs of positional isomers of unsaturated mixed-acid phosphatidylcholines.

    abstract：:The gel to liquid-crystalline phase transition temperatures of dispersions of mixed-acid sn-1,2-lecithins which contain one unsaturated and one saturated fatty acid have been studied by differential scanning calorimetry. The temperature for 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (containing no reversed isome...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00515a051

    authors： Davis PJ,Fleming BD,Coolbear KP,Keough KM

    更新日期：1981-06-09 00:00:00

  • Interactions of a Bacterial Cu(I)-ATPase with a Complex Lipid Environment.

    abstract：:Phospholipids and sterols play multiple roles in cells. In addition to establishing barriers between compartments, they also provide the matrix for assembly and function of a large variety of catalytic processes. Lipid composition is a highly regulated feature of biological membranes, yet its implications for membrane...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00326

    authors： Autzen HE,Koldsø H,Stansfeld PJ,Gourdon P,Sansom MSP,Nissen P

    更新日期：2018-07-17 00:00:00

  • Interaction of endothelin-1 with cloned bovine ETA receptors: biochemical parameters and functional consequences.

    abstract：:This paper defines the properties of interaction of endothelin-1 (Et-1) with cloned bovine ETA receptors. The Kd value of Et-1/ETA receptor complexes was estimated in membrane preparations to 20 pM using kinetic experiments and saturation experiments performed under quasi equilibrium conditions. Competition experiment...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961238w

    authors： Desmarets J,Gresser O,Guedin D,Frelin C

    更新日期：1996-11-26 00:00:00