Abstract：
:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size-exclusion high-performance liquid chromatography and sedimentation equilibrium studies indicated that acetylated inner core histones associate to species larger than the octamer and form a more stable complex. Circular dichroism studies demonstrated that the amount of alpha-helix increases with increasing association of the histones. Furthermore, acetylation results in an increase in the amount of alpha-helix, perhaps coupled through its effect on the association state. At high protein concentration and elevated temperature, the acetylated sample displays a greater increase in beta-sheet content, relative to the control sample. This increase in beta-sheet content may be induced during the association of the acetylated sample to species larger than the octamer. There is a marked effect on the conformation of both acetylated and control inner core histones as a function of protein concentration, ionic strength, and temperature. The difference in conformational flexibility and association state of the acetylated vs. the control inner histone core may play a significant role in the control of transcription in the nucleus.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Prevelige PE Jr,Fasman GDdoi
10.1021/bi00384a041subject
Has Abstractpub_date
1987-05-19 00:00:00pages
2944-55issue
10eissn
0006-2960issn
1520-4995journal_volume
26pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970177e
更新日期：1997-08-19 00:00:00
abstract：:Lipid species changes for SV40-transformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice were determined for glycerophospholipids, polyphosphatidylinositides (GPInsP n ) and diacylglycerol (DAG) using direct infusion mass spectrome...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800492c
更新日期：2008-09-09 00:00:00
abstract：:Pig liver phosphomevalonate kinase (EC 2.7.4.2) has been purified to homogeneity as shown by polyacrylamide gel electrophoresis. The molecular weight estimates range from 21,000 to 22,500. Each molecule is composed of one polypeptide chain. The presence of SH-containing reagents is essential for the preservation of en...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00552a003
更新日期：1980-05-27 00:00:00
abstract：:The role of histidine in catalysis by prostaglandin H synthase has been investigated using chemical modification with diethyl pyrocarbonate (DEPC), an agent that has been found to rather selectively derivatize histidine residues in proteins under mild conditions. Incubation of the synthase apoprotein with DEPC at pH 7...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00124a013
更新日期：1992-03-10 00:00:00
abstract：:Seven mutant LH1 polypeptides of Rhodobactor sphaeroides have been isolated, and their behaviors in in vitro reconstitution of LH1 and its subunit complex have been characterized. Two mutants were selected to address the increased stability of the subunit complex of Rb. sphaeroides compared with that of Rhodobacter ca...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962386p
更新日期：1997-03-25 00:00:00
abstract：:The sequences and three-dimensional structures of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family were compared to identify highly conserved surface residues. The functions of these solvent-accessible residues were assessed by determining the effects of their substit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048963o
更新日期：2004-11-23 00:00:00
abstract：:The crystal structures of the guanosine 5'-monophosphate (GMP) and inosine 5'-monophosphate (IMP) complexes of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase (HGPRT) have been determined at 1.65 and 1.90 A resolution. These complexes, which crystallize in space groups P2(1) (a = 65.45 A, b = 90.84 A,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990507q
更新日期：1999-11-02 00:00:00
abstract：:Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951577+
更新日期：1996-01-09 00:00:00
abstract：:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900064t
更新日期：2009-06-02 00:00:00
abstract：:His216 is a well-conserved residue in pyruvate carboxylases and, on the basis of structures of the enzyme, appears to have a role in the binding of MgATP, forming an interaction with the 3'-hydroxyl group of the ribose ring. Mutation of this residue to asparagine results in a 9-fold increase in the Km for MgATP in its...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4016814
更新日期：2014-02-18 00:00:00
abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300295y
更新日期：2012-04-24 00:00:00
abstract：:The thermodynamic binding parameters and crystal structure for streptavidin-peptide complexes where the peptide sequences were obtained by random screening methods are reported. The affinities between streptavidin and two heptapeptides were determined by titrating calorimetric methods [Phe-Ser-His-Pro-Gln-Asn-Thr, Ka ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00154a004
更新日期：1992-10-06 00:00:00
abstract：:The Radical SAM (RS) enzyme PqqE catalyzes the first step in the biosynthesis of the bacterial cofactor pyrroloquinoline quinone, forming a new carbon-carbon bond between two side chains within the ribosomally synthesized peptide substrate PqqA. In addition to the active site RS 4Fe-4S cluster, PqqE is predicted to ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01097
更新日期：2018-02-27 00:00:00
abstract：:DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00624a016
更新日期：1977-03-08 00:00:00
abstract：:Arginase is a binuclear manganese metalloenzyme that serves as a therapeutic target for the treatment of asthma, erectile dysfunction, and atherosclerosis. In order to better understand the molecular basis of inhibitor affinity, we have employed site-directed mutagenesis, enzyme kinetics, and X-ray crystallography to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801911v
更新日期：2009-01-13 00:00:00
abstract：:The following peptide was synthesized by classical methods in solution: Ac-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg-Gly-Pro-Arg-Val-NHCH3 (F-8). The Michaelis-Menten parameters for the hydrolysis of the Arg-Gly bond in F-8 by thrombin were determined to be Kcat = 31 X 10(-11) M [(NIH unit/L) s]-1 and KM = 310 X 10(-6) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00287a002
更新日期：1983-08-30 00:00:00
abstract：:Folding experiments have suggested that some proteins have kinetic intermediates with a non-native structure. A simple G ̅o model does not explain such non-native intermediates. Therefore, the folding energy landscape of proteins with non-native intermediates should have characteristic properties. To identify such pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200241a
更新日期：2011-07-26 00:00:00
abstract：:Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00607a034
更新日期：1978-07-11 00:00:00
abstract：:Monte Carlo protein simulations with continuum solvation were used to explore the conformational mobility of NO within the active site of metmyoglobin. To the best of our knowledge this is the first application of a continuum solvation model for exploring protein binding sites. The usefulness of the Monte Carlo confor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981611v
更新日期：1999-05-18 00:00:00
abstract：:Factors which influence the accessibility, or exposure, of the carbohydrate head group of the glycolipid galactosylceramide (GalCer) at the membrane surface have been examined in lipid model membranes using the technique of galactose oxidase-tritiated sodium borohydride labeling. Both the ceramide composition of GalCe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00072a016
更新日期：1993-06-01 00:00:00
abstract：:One- and two-dimensional NMR spectroscopy and energy minimization calculations were used to investigate the conformation of a 2-aminofluorene- (AF-) modified model human c-H-ras1 protooncogene codon 61 deoxyoligonucleotide duplex, d(C1-A2-C3-C4-A5- [AF-G6]-G7-A8-A9-C10).-d(G11-T12-T13-C14-C15-T16 -G17-G18-T19-G20), in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00250a012
更新日期：1994-11-22 00:00:00
abstract：:Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980565w
更新日期：1998-08-25 00:00:00
abstract：:Simple procedures for the anaerobic preparation of photoactive and stable P840 reaction centers from Chlorobium tepidum and Chlorobium limicola in good yield are presented and quantitated. The subunit composition was tested by cosedimentation in sucrose density gradients. For C. limicola, it minimally comprises four s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00029a039
更新日期：1995-07-25 00:00:00
abstract：:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00432a003
更新日期：1989-03-21 00:00:00
abstract：:Azotobacter vinelandii bacterioferritin (AvBF) containing 800-1500 Co or Mn atoms as Co(III) and Mn(III) oxyhydroxide cores (Co-AvBF, Mn-AvBF) was synthesized by the same procedure used previously for horse spleen ferritin (HoSF). The kinetics of reduction of Co-AvBF and Mn-AvBF by ascorbic acid are first-order in eac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060164d
更新日期：2006-05-09 00:00:00
abstract：:A synthetic analogue of the tripeptide hemiasterlin, designated HTI-286, depolymerizes microtubules, is a poor substrate for P-glycoprotein, and inhibits the growth of paclitaxel-resistant tumors in xenograft models. Two radiolabeled photoaffinity analogues of HTI-286, designated 4-benzoyl-N,beta,beta-trimethyl-l-phen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0474766
更新日期：2005-05-10 00:00:00
abstract：:Human lung tryptase (HLT), a trypsin-like serine proteinase stored as an active enzyme in association with heparin in mast cell granules, is released into the extracellular environment when mast cells are activated. Tryptases are unusual in that they form tetramers and bind heparin. As there are no known endogenous tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960042t
更新日期：1996-10-22 00:00:00
abstract：:Legumain (asparaginyl endopeptidase) is the only protease with a preference for cleavage after asparagine residues. Increased legumain activity is a hallmark of inflammation, neurodegenerative diseases, and cancer, and legumain inhibitors have exhibited therapeutic effects in mouse models of these pathologies. Improve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00821
更新日期：2020-01-28 00:00:00
abstract：:Ultraviolet resonance Raman (RR) spectra, with 200- and 218-nm excitation from a H2-shifted quadrupled Nd:YAG laser, are reported for insulin and alpha-lactalbumin in dilute aqueous solution, at pH values known to produce differences in the exposure of the aromatic residues to solvent. At 200 nm, the spectra are domin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00329a009
更新日期：1985-04-09 00:00:00
abstract：:The fate of plasma and nuclear membrane polypeptides in preparations of acidic chromosomal protein from chicken erythrocytes has been investigated. It is shown that detergent extraction procedures (Nonidet P-40, Triton X-100, and saponin), commonly employed in the preparation of acidic chromosomal protein, cannot be r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00670a034
更新日期：1976-12-14 00:00:00
