Mechanism of action of thrombin on fibrinogen. Kinetic evidence for involvement of aspartic acid at position P10.

abstract：:Gemcitabine 5'-diphosphate (F(2)CDP) is a potent inhibitor of ribonucleotide reductases (RNRs), enzymes that convert nucleotides (NDPs) to deoxynucleotides and are essential for DNA replication and repair. The Escherichia coli RNR, an alpha2beta2 complex, when incubated with 1 equiv of F(2)CDP catalyzes the release of...

journal_title：Biochemistry

authors： Artin E,Wang J,Lohman GJ,Yokoyama K,Yu G,Griffin RG,Bar G,Stubbe J

更新日期：2009-12-15 00:00:00

abstract：:The primary structure of the 43-kilodalton peripheral membrane protein (43-kDa protein) of Torpedo nicotinic postsynaptic membrane has been determined. The 43-kDa protein, which was isolated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, has an amino terminus resistant to Edman degradation, ...

journal_title：Biochemistry

authors： Carr C,McCourt D,Cohen JB

更新日期：1987-11-03 00:00:00

abstract：:Tafazzin is the mitochondrial enzyme that catalyzes transacylation between a phospholipid and a lysophospholipid in remodeling. Mutations in tafazzin cause Barth syndrome, a potentially life-threatening disease with the major symptom being cardiomyopathy. In the tafazzin-deficient heart, cardiolipin (CL) acyl chains b...

journal_title：Biochemistry

authors： Kimura T,Kimura AK,Ren M,Berno B,Xu Y,Schlame M,Epand RM

更新日期：2018-04-10 00:00:00

abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...

journal_title：Biochemistry

authors： Chartrand P,Usman N,Cedergren R

更新日期：1997-03-18 00:00:00

abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

journal_title：Biochemistry

authors： Jelicks LA,Broido MS,Becker JM,Naider FR

更新日期：1989-05-16 00:00:00

abstract：:A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...

journal_title：Biochemistry

authors： Rad B,Kowalczykowski SC

更新日期：2012-04-03 00:00:00

abstract：:Glycogen phosphorylase is a muscle enzyme which metabolizes glycogen, producing glucose-1-phosphate, which can be used for the production of ATP. Phosphorylase activity is regulated by phosphorylation/dephosphorylation, and by the allosteric binding of numerous effectors. In this work, we have studied 10 site-directed...

journal_title：Biochemistry

authors： Biorn AC,Graves DJ

更新日期：2001-05-01 00:00:00

abstract：:Enzyme IIA(Glc) (EIIA(Glc)) of the phosphoenolpyruvate phosphotransferase system for the uptake of glucose in Escherichia coli and Salmonella inhibits the maltose ATP-binding cassette transporter (MalE-FGK2) by interaction with the nucleotide-binding and -hydrolyzing subunit MalK, a process termed inducer exclusion. W...

journal_title：Biochemistry

authors： Wuttge S,Licht A,Timachi MH,Bordignon E,Schneider E

更新日期：2016-09-27 00:00:00

abstract：:The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...

journal_title：Biochemistry

authors： Mizushina Y,Ohkubo T,Sugawara F,Sakaguchi K

更新日期：2000-10-17 00:00:00

abstract：:Using a simple model for a biological membrane we examine cation-induced gel phase formation and the depletion of polypeptide from the gel phase. The model system consists of vesicles of phosphatidic acid and phosphatidylcholine which contain gramicidin A'. By use of electron spin resonance to monitor lipid phase beha...

journal_title：Biochemistry

authors： Feigenson GW

更新日期：1983-06-21 00:00:00

abstract：:The tau microtubule-associated proteins are axonal proteins that have been implicated in axonal outgrowth, microtubule spacing, and microtubule bundling. Moreover, tau is the major structural component of the paired helical filaments present in the brains of Alzheimer's disease patients. The Caenorhabditis elegans Gen...

journal_title：Biochemistry

authors： McDermott JB,Aamodt S,Aamodt E

更新日期：1996-07-23 00:00:00

abstract：:A high molecular weight inert molecule, poly(ethylene glycol) (PEG), or a soluble protein, ovalbumin, causes parallel bundles of actin filaments in a crystalline-like structure under physiological conditions of ionic compositions and pH. The bundle formation depends on the molecular weight of PEG, and a larger molecul...

journal_title：Biochemistry

authors： Suzuki A,Yamazaki M,Ito T

更新日期：1989-07-25 00:00:00

abstract：:Triple helix-forming oligonucleotides (TFOs) bind with high affinity and specificity to homopurine-homopyrimidine sequences in DNA and have been shown to inhibit transcription of target genes in various experimental systems. In the present study, we evaluated the ability of 3'-amino-modified phosphodiester TFOs direct...

journal_title：Biochemistry

authors： Catapano CV,McGuffie EM,Pacheco D,Carbone GM

更新日期：2000-05-02 00:00:00

abstract：:The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediat...

journal_title：Biochemistry

authors： Simmons CR,Krishnamoorthy K,Granett SL,Schuller DJ,Dominy JE Jr,Begley TP,Stipanuk MH,Karplus PA

更新日期：2008-11-04 00:00:00

abstract：:During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...

journal_title：Biochemistry

authors： Casy W,Prater AR,Cornish PV

更新日期：2018-04-03 00:00:00

abstract：:Colibactins are genotoxic secondary metabolites whose biosynthesis is encoded in the clb gene cluster harbored by certain strains of gut commensal Escherichia coli. Using synthetic colibactin analogues, we previously provided evidence that colibactins alkylate DNA by addition of a nucleotide to an electrophilic cyclop...

journal_title：Biochemistry

authors： Xue M,Shine E,Wang W,Crawford JM,Herzon SB

更新日期：2018-11-13 00:00:00

abstract：:Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...

journal_title：Biochemistry

authors： Lemkul JA,Bevan DR

更新日期：2012-07-31 00:00:00

abstract：:The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA by the removal of misincorporated adenine residues in OG:A mispairs. MutY also exhibits adenine glycosylase activity toward...

journal_title：Biochemistry

authors： Porello SL,Leyes AE,David SS

更新日期：1998-10-20 00:00:00

abstract：:The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

journal_title：Biochemistry

authors： Reisler E,Liu J,Cheung P

更新日期：1983-10-11 00:00:00

abstract：:5-HT(2A) serotonin receptors represent the principal molecular targets for LSD-like hallucinogens and atypical antipsychotic drugs. It has been proposed that a dysregulation of 5-HT(2A) receptor-mediated signaling may contribute to the pathogenesis of schizophrenia and related diseases. A major mechanism for the atten...

journal_title：Biochemistry

authors： Gray JA,Compton-Toth BA,Roth BL

更新日期：2003-09-16 00:00:00

abstract：:The chromatin structure encompassing the lysozyme gene domain in hen oviduct nuclei was studied by measuring the partitioning of coding and flanking sequences during chromatin fractionation and by analyzing the nucleosome repeat in response to micrococcal nuclease digestion. Following micrococcal nuclease digestion, n...

journal_title：Biochemistry

authors： Strätling WH,Dölle A,Sippel AE

更新日期：1986-01-28 00:00:00

abstract：:Reticulocyte lysate contains a chaperone system that assembles glucocorticoid receptor (GR).hsp90 heterocomplexes. Using purified proteins, we have prepared a five-protein heterocomplex assembly system consisting of two proteins essential for heterocomplex assembly-hsp90 and hsp70-and three proteins that act as co-cha...

journal_title：Biochemistry

authors： Kanelakis KC,Murphy PJ,Galigniana MD,Morishima Y,Takayama S,Reed JC,Toft DO,Pratt WB

更新日期：2000-11-21 00:00:00

abstract：:The positions of single amino acids in the interhelical loop regions and the C-terminal tail of bacteriorhodopsin (bR) were investigated by X-ray diffraction using site-directed heavy-atom labeling. Since wild-type bR does not contain any cysteines, appropriate cysteine mutants were produced with a unique sulfhydryl g...

journal_title：Biochemistry

authors： Behrens W,Alexiev U,Mollaaghababa R,Khorana HG,Heyn MP

更新日期：1998-07-21 00:00:00

abstract：:Mg2+ is important for the RNase activity of the hammerhead ribozyme. To investigate the binding properties of Mg2+ to the hammerhead ribozyme, cleavage rates and CD spectra for substrates containing inosine or guanosine at the cleavage site were measured. The 2-amino group of this guanosine interfered with the rate of...

journal_title：Biochemistry

authors： Koizumi M,Ohtsuka E

更新日期：1991-05-28 00:00:00

abstract：:The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopi...

journal_title：Biochemistry

authors： Jiménez-García E,Portugal J

更新日期：1992-11-24 00:00:00

abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...

journal_title：Biochemistry

authors： Dwulet FE,Jones BN,Lehman LD,Gurd FR

更新日期：1977-03-08 00:00:00

abstract：:A procedure has been developed to purify foot and mouth disease virus (FMDV) VP1 surface antigens from recombinant Escherichia coli. The VP1 antigens are expressed as fusion proteins derived from the E. coli Trp operon and VP1 surface protein of FMDV. The procedure is capable of recovering greater than 96% of the desi...

journal_title：Biochemistry

authors： Shire SJ,Bock L,Ogez J,Builder S,Kleid D,Moore DM

更新日期：1984-12-18 00:00:00

abstract：:Xylose isomerase (E.C. 5.3.1.5) catalyzes the interconversion of aldose and ketose sugars and has an absolute requirement for two divalent cations at its active site to drive the hydride transfer rates of sugar isomerization. Evidence suggests some degree of metal movement at the second metal site, although how this m...

journal_title：Biochemistry

authors： Fenn TD,Ringe D,Petsko GA

更新日期：2004-06-01 00:00:00

abstract：:The temperature dependence of biological rates at different scales (from individual enzymes to isolated organisms to ecosystem processes such as soil respiration and photosynthesis) is the subject of much historical and contemporary research. The precise relationship between the temperature dependence of enzyme rates ...

journal_title：Biochemistry

authors： Prentice EJ,Hicks J,Ballerstedt H,Blank LM,Liáng LNL,Schipper LA,Arcus VL

更新日期：2020-09-29 00:00:00

abstract：:Long-chain alkanols are general anesthetics which can also act as uncharged noncompetitive inhibitors of the peripheral nicotinic acetylcholine receptor (AChR) by binding to one or more specific sites on the AChR. Cembranoids are naturally occurring, uncharged noncompetitive inhibitors of peripheral and neuronal AChRs...

journal_title：Biochemistry

authors： Pagán OR,Eterović VA,Garcia M,Vergne D,Basilio CM,Rodríguez AD,Hann RM

更新日期：2001-09-18 00:00:00