Abstract：
:Genomic clones encompassing all the translated sequences, the 3' untranslated sequence, and 1 kb flanking the ATG translation initiation codon of bovine tropoelastin have been obtained and characterized by restriction enzyme analysis and extensive DNA sequencing. These analyses demonstrated that functionally distinct hydrophobic and cross-linking domains of the protein are segregated into separate exons throughout the gene. The putative promoter region lacks a TATA box, has an extremely high G+C content, and contains several SP1 binding sites. Comprehensive S1 analyses using probes covering the entire mRNA and RNA isolated from the nuchal ligament of bovine fetuses of different ages, neonate calves, and adult cows demonstrated that while only a single exon is alternatively spliced at high frequency, many exons are alternatively spliced at limited, variable frequencies. The results also suggest that such limited splicing is increased in the adult tissue relative to fetal and neonate tissues.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Yeh H,Anderson N,Ornstein-Goldstein N,Bashir MM,Rosenbloom JC,Abrams W,Indik Z,Yoon K,Parks W,Mecham Rdoi
10.1021/bi00432a003subject
Has Abstract,Author List Incompletepub_date
1989-03-21 00:00:00pages
2365-70issue
6eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The interaction of Triton X-100 and other nonionic detergents with a delipidated preparation of the Ca2+ ATPase from sarcoplasmic reticulum has been studied. Binding of radiolabeled Triton X-100 was determined by column chromatography at 6 degrees C, and two classes of binding sites were observed. Below the critical m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00510a006
更新日期：1981-03-31 00:00:00
abstract：:The reactivity of aluminum and several other divalent and trivalent metallic cations toward chromatin from rat brain and liver has been investigated. Two criteria are used to determine the relative reactivity of these cations toward chromatin. The first involves the ability of the ions to compact the chromatin fibers ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00435a043
更新日期：1989-05-02 00:00:00
abstract：:Steric constraints in the retinal binding pocket of sensory rhodopsin I (SR-I) are analyzed by studying effects of sample temperature and retinal analogs. The flash-induced yield of the earliest detected intermediate S610, which corresponds to the K intermediate in the bacteriorhodopsin (BR) photocycle, decreases belo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00089a044
更新日期：1993-09-28 00:00:00
abstract：:We have previously designed and synthesized small-molecule inhibitors that reduce Vibrio cholerae virulence in vitro by targeting the transcription factor ToxT. Here we report the synthesis and biological activity of derivatives of our previous bicyclic, fatty acid-like inhibitors. All of the synthesized derivatives s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00667
更新日期：2018-09-25 00:00:00
abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6026192
更新日期：2007-04-10 00:00:00
abstract：:Hydrogen bonds which form between a hydrogen bond donor and an aromatic ring as acceptor are thought to contribute to the stability and function of proteins. We have tested the function of such an interaction in a highly homologous pair of proteins, cellular retinol-binding protein (CRBP) and cellular retinol-binding ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00035a018
更新日期：1995-09-05 00:00:00
abstract：:Electrophilic halogenating agents, including hypohalous acids and haloamines, oxidize free methionine and the N-terminal methionines of peptides and proteins (e.g., Met-1 of anti-inflammatory peptide 1 and ubiquitin) to produce dehydromethionine (a five-membered isothiazolidinium heterocycle). Amide derivatives of met...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901343d
更新日期：2009-11-24 00:00:00
abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963133z
更新日期：1997-05-06 00:00:00
abstract：:The GTP-binding proteins or GTPases comprise a superfamily of proteins that provide molecular switches in numerous cellular processes. The "GTPase switch" paradigm, in which a GTPase acts as a bimodal switch that is turned "on" and "off" by external regulatory factors, has been used to interpret the regulatory mechani...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9006989
更新日期：2009-07-28 00:00:00
abstract：:A visual pigment consists of an opsin protein and a chromophore, 11-cis-retinal, which binds to a specific lysine residue of opsin via a Schiff base linkage. The Schiff base chromophore is protonated in pigments that absorb visible light, whereas it is unprotonated in ultraviolet-absorbing visual pigments (UV pigments...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7003763
更新日期：2007-05-29 00:00:00
abstract：:Previous work from this laboratory has established that the NADPH-menadione oxidoreductase reaction catalyzed by methylenetetrahydrofolate reductase from pig liver proceeds by Ping Pong Bi Bi kinetics and that the reductive half-reaction is rate limiting in steady-state turnover. We have now shown that methylenetetrah...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00317a027
更新日期：1984-10-23 00:00:00
abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a030
更新日期：1975-09-23 00:00:00
abstract：:The interaction of the C-terminal octapeptide of cholecystokinin, CCK-8, with the third extracellular loop of human cholecystokinin-A receptor, CCK(A)-R(329-357), has been probed by high-resolution NMR and extensive computer simulations. The structure of CCK(A)-R(329-357) in the presence of dodecylphosphocholine micel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002659n
更新日期：2001-04-03 00:00:00
abstract：:Deazapurine-containing secondary metabolites comprise a broad range of structurally diverse nucleoside analogues found throughout biology, including various antibiotics produced by species of Streptomyces bacteria and the hypermodified tRNA bases queuosine and archaeosine. Despite early interest in deazapurines as ant...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900400e
更新日期：2009-05-12 00:00:00
abstract：:The transcriptional activity of the beta-globin genes is regulated by a complex genetic element, the locus control region (LCR), at the 5'-end of the beta-globin locus. Tandem binding sites for the erythroid-specific transcription factor NF-E2 are important for the transcriptional activation function of the LCR. We di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00050a015
更新日期：1995-12-19 00:00:00
abstract：:In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501160q
更新日期：2015-01-20 00:00:00
abstract：:Herpesvirus saimiri codes for a tyrosine kinase interacting protein (Tip) that interacts with both the SH3 domain and the kinase domain of the T-cell-specific tyrosine kinase Lck via two separate motifs. The activation of Lck by Tip is considered as a key event in the transformation of human T-lymphocytes during herpe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015986j
更新日期：2002-04-23 00:00:00
abstract：:Helix-helix interactions within membranes are dominated by van der Waals packing motifs and side chain-side chain hydrogen bond formation, which act in tandem to determine the residues that comprise the interface between two given helices. To explore in a systematic manner the tertiary contacts between transmembrane h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0492760
更新日期：2004-11-16 00:00:00
abstract：:The effect of DnaB helicase on the initiation specificity of primase was studied biochemically using a series of single-stranded DNA templates in which each nucleotide of the trinucleotide d(CTG) initiation sequence was systematically varied. DnaB helicase accelerated the rate of primer syntheisis, prevented "overlong...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991555d
更新日期：2000-02-01 00:00:00
abstract：:Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800164c
更新日期：2008-05-06 00:00:00
abstract：:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The resul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00406a021
更新日期：1988-03-22 00:00:00
abstract：:The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Escherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthrol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00216a033
更新日期：1991-01-15 00:00:00
abstract：:The expression of rusticyanin in Escherichia coli and a number of mutants for Ser86 is reported. Mutations of Ser86 to Asn, Asp, Gln, and Leu were undertaken as this is an Asn residue in other structurally characterized cupredoxins, and it has been suggested that this may be partly responsible for the high redox poten...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980960m
更新日期：1998-08-18 00:00:00
abstract：:A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00584a026
更新日期：1979-08-21 00:00:00
abstract：:The time course of changes in the intravesicular Ca2+ concentration ([Ca2+]i) in terminal cisternal sarcoplasmic reticulum vesicles upon the induction of Ca2+ release was investigated by using tetramethylmurexide (TMX) as an intravesicular Ca2+ probe. Upon the addition of polylysine at the concentration that led to th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00235a017
更新日期：1991-05-28 00:00:00
abstract：:The primary structure of a multifunctional protein, the large alpha-subunit of the Escherichia coli fatty acid oxidation complex, was determined by sequencing the fadB region of the fadBA operon. The amino-terminal sequence of this protein had been established by Edman degradation. The transcription start site of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00241a023
更新日期：1991-07-09 00:00:00
abstract：:A series of diastereomeric dipeptide amides, containing an N-terminal L-lysyl residue and a C-terminal L- or D-amino acid with a derivatized aromatic ring on the side chain, was synthesized to determine the dependence of (1) the chirality of the N-terminal amino acid alpha-carbon and (2) the length of the N-terminal a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00278a005
更新日期：1983-04-26 00:00:00
abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200104h
更新日期：2011-05-31 00:00:00
abstract：:A full-length cDNA for dopamine beta-monooxygenase (D beta M) from bovine adrenal glands has been cloned and sequenced. The soluble and membrane-derived forms of D beta M have also been sequenced from their N-termini. While the observed sequences for the soluble protein correspond to those previously reported [Joh, T....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00452a026
更新日期：1989-12-26 00:00:00
abstract：:Linker histones bind to the nucleosomes and linker DNA of chromatin fibers, causing changes in linker DNA structure and stabilization of higher order folded and oligomeric chromatin structures. Linker histones affect chromatin structure acting primarily through their approximately 100-residue C-terminal domain (CTD). ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801636y
更新日期：2009-01-13 00:00:00
