Abstract:
:A visual pigment consists of an opsin protein and a chromophore, 11-cis-retinal, which binds to a specific lysine residue of opsin via a Schiff base linkage. The Schiff base chromophore is protonated in pigments that absorb visible light, whereas it is unprotonated in ultraviolet-absorbing visual pigments (UV pigments). To investigate whether an unprotonated Schiff base can undergo photoisomerization as efficiently as a protonated Schiff base in the opsin environment, we measured the quantum yields of the bovine rhodopsin E113Q mutant, in which the Schiff base is unprotonated at alkaline pH, and the mouse UV pigment (mouse UV). Photosensitivities of UV pigments were measured by irradiation of the pigments followed by chromophore extraction and HPLC analysis. Extinction coefficients were estimated by comparing the maximum absorbances of the original pigments and their acid-denatured states. The quantum yield of the bovine rhodopsin E113Q mutant at pH 8.2, where the Schiff base is unprotonated, was significantly lower than that of wild-type rhodopsin, whereas the mutant gave a quantum yield almost identical to that of the wild type at pH 5.5, where the Schiff base is protonated. These results suggest that Schiff base protonation plays a role in increasing quantum yield. The quantum yield of mouse UV, which has an unprotonated Schiff base chromophore, was significantly higher than that of the unprotonated form of the rhodopsin E113Q mutant, although it was still lower than the visible-absorbing pigments. These results suggest that the mouse UV pigment has a specific mechanism for the efficient photoisomerization of its unprotonated Schiff base chromophore.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tsutsui K,Imai H,Shichida Ydoi
10.1021/bi7003763subject
Has Abstractpub_date
2007-05-29 00:00:00pages
6437-45issue
21eissn
0006-2960issn
1520-4995journal_volume
46pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was found to consist of four alpha-helical regions and a short sectio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00148a040
更新日期:1992-08-25 00:00:00
abstract::Familial British dementia (FBD) is a rare neurodegenerative disorder and shares features with Alzheimer's disease, including amyloid plaque deposits, neurofibrillary tangles, neuronal loss, and progressive dementia. Immunohistochemical and biochemical analysis of plaques and vascular amyloid of FBD brains revealed tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002287i
更新日期:2001-03-27 00:00:00
abstract::The MexA-MexB-OprM efflux pump exports structurally and functionally diverse xenobiotics and confers multi-drug resistance on Pseudomonas aeruginosa cells. The MexB transporter traverses the inner membrane twelve times, bears two large periplasmic domains and has two homologous tandem repeats. To test whether two homo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0300074
更新日期:2003-06-17 00:00:00
abstract::Despite extensive characterization of the human immunodeficiency virus type 1 (HIV-1) hydrophobic fusion peptide (FP), the structure-function relationships underlying its extraordinary degree of conservation remain poorly understood. Specifically, the fact that the tandem repeat of the FLGFLG tripeptide is absolutely ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00745
更新日期:2017-10-17 00:00:00
abstract::The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein canno...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00038a003
更新日期:1995-09-26 00:00:00
abstract::Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034394t
更新日期:2003-06-24 00:00:00
abstract::6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0267994
更新日期:2003-02-18 00:00:00
abstract::The promoter fragment -1550/+129 of the human hepatic triglyceride lipase (HTGL) gene drives the expression of the CAT gene in HepG2 cells, albeit at very low levels. Transient transfections in HepG2 and HeLa cells of 5' deletion constructs indicated that the regulatory elements that control this expression are locate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00088a018
更新日期:1993-09-21 00:00:00
abstract::The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00117a033
更新日期:1992-01-21 00:00:00
abstract::Trinucleotide repeat expansion diseases (TREDs) are correlated with elongation of CNG DNA and RNA repeats to pathological level. This paper shows, for the first time, complete data concerning thermodynamic stabilities of RNA with CNG trinucleotide repeats. Our studies include the stability of oligoribonucleotides comp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0502339
更新日期:2005-08-16 00:00:00
abstract::Traditionally, assigning the heme protein resonances has relied heavily on the comparison of spectra arising from protein reconstituted with specifically deuterated hemes and the native form. Such an approach can identify tentatively the broad, overlapping signals in the Fe(II) high-spin heme protein spectra. Although...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00202a012
更新日期:1994-09-13 00:00:00
abstract::Transport of lactate, pyruvate, and the ketone bodies, acetoacetate and beta-hydroxybutyrate, is mediated in many mammalian cells by the monocarboxylate transporter MCT1. To be accepted as a substrate, a carboxyl group and an unpolar side chain are necessary. Site-directed mutagenesis of the rat MCT1 was used to ident...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990973f
更新日期:1999-08-31 00:00:00
abstract::Cu,Zn superoxide dismutase from Photobacterium leiognathi has been cloned and expressed in Escherichia coli. The circular dichroism spectrum in the UV region of the recombinant protein indicates an higher content of random coil structure with respect to the eukaryotic enzymes. Investigation of the active site by optic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963020f
更新日期:1997-06-10 00:00:00
abstract::Several bifunctional alkylating agents of the aziridinylbenzoquinone class have been evaluated as potential antitumor agents. 3,6-Bis[(2-hydroxyethyl)amino]-2,5- diaziridinyl-1,4-benzoquinone (BZQ), 2,5-diaziridinyl-1,4-benzoquinone (DZQ), 3,6-bis(carboxyamino)-2,5-diaziridinyl- 1,4-benzoquinone (AZQ), and six analogu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00114a016
更新日期:1991-12-17 00:00:00
abstract::The highly conserved amino acid sequence PCDGPGRGGTC in both photosystem I reaction center core proteins PsaA and PsaB has been predicted to contribute the four cysteine ligands for coordination of the 4Fe-4S iron-sulfur cluster FX, and we have proposed a working model for the binding of PsaC to this domain of the rea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00019a010
更新日期:1995-05-16 00:00:00
abstract::Plasma membrane and vesicular transporters for the biogenic amines, dopamine, norepinephrine, and serotonin, represent a group of proteins that play a crucial role in the regulation of neurotransmission. Clinically, mono amine transporters are the primary targets for the actions of many therapeutic agents used to trea...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi200405c
更新日期:2011-08-30 00:00:00
abstract::The equilibrium between the rat brain tubulin alpha beta dimer and the dissociated alpha and beta monomers has been studied by analytical ultracentrifugation with use of a new method employing short solution columns, allowing rapid equilibration and hence short runs, minimizing tubulin decay. Simultaneous analysis of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00228a023
更新日期:1991-04-09 00:00:00
abstract::Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00425a007
更新日期:1988-12-13 00:00:00
abstract::Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purifie...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00496a008
更新日期:1990-11-06 00:00:00
abstract::The proline catabolic enzyme Δ(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) catalyzes the NAD(+)-dependent oxidation of γ-glutamate semialdehyde to l-glutamate. In Saccharomyces cerevisiae, ALDH4A1 is encoded by the PUT2 gene and known as Put2p. Here we report the steady-state kinetic parameters of the purified ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500048b
更新日期:2014-03-04 00:00:00
abstract::Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051658y
更新日期:2005-12-20 00:00:00
abstract::The quercetin binding sites on spinach chloroplast coupling factor 1 (CF1) have been investigated using direct and competitive binding, stopped-flow, temperature-jump, and fluorescence resonance energy transfer measurements. It was found that 8-anilino-1-naphthalensulfonic acid (ANS) competes with quercetin binding at...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00646a001
更新日期:1976-01-13 00:00:00
abstract::Cells can be transiently permeabilized by application of high electric pulses of short duration. A direct consequence of this treatment is to induce a fusogenic state in the pulsed membrane. The molecular events underlying these phenomena remain to be explained. During our work, we investigated the effects of pulsing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00471a009
更新日期:1990-05-15 00:00:00
abstract::Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00135a022
更新日期:1992-05-26 00:00:00
abstract::Nine monoclonal antibodies were used to test calcium and EDTA effects on the molecular conformation of ventricular VLC2 within myosin. Antibody epitopes were located in six domains of VLC2 using recombinant proteins. The apparent association constants of these antibodies were measured in solution in the presence of ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00208a005
更新日期:1994-10-25 00:00:00
abstract::The hydrogen exchange kinetics of the complex of trypsin-soybean trypsin inhibitor (Kunitz) have been compared to the calculated sum of the exchange kinetics for the inhibitor and trypsin measured separately. The exchange rates observed for the complex are substantially less than the sum of the exchange rates in the t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00686a020
更新日期:1975-07-29 00:00:00
abstract::Electron transfer in reaction center core (RCC) complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum was studied by measuring flash-induced absorbance changes. The first preparation contained approximately three iron-sulfur centers, indicating that the three putative electron acce...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992861u
更新日期:2000-06-20 00:00:00
abstract::Sarcosine oxidase from Corynebacterium sp. P-1 binds 2 mol of tetrahydrofolate/mol of enzyme (KD = 8.8 microM). The same stoichiometry is observed with tetrahydropteroyltetraglutamate (KD = 15.4 microM). Binding is also observed with pteroyltetraglutamate and with 5-formyltetrahydrofolate. In the case of the pteroylmo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00397a029
更新日期:1987-11-17 00:00:00
abstract::Peptides prepared from the amino termini of pro alpha 1(I) and pro alpha 1(III) collagen chains inhibit the production of pro alpha 1(I) and pro alpha 2 by rat calvaria rna in a reticulocyte cell-free system. The synthesis of other proteins was not altered, suggesting a specific effect on collagen production. Various ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00589a034
更新日期:1979-10-30 00:00:00
abstract::Photochemical studies of the effects of temperature, pH, and dehydration on the formation and back photoreaction of the M412 intermediate in the photocycle of light-adapted bacteriorhodopsin (bR570) are carried out. Continuous illumination experiments in the range between -40 and -90 degrees C indicate that at low tem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00506a031
更新日期:1981-02-03 00:00:00