Abstract：
:Insulin is very prone to form amyloid fibrils under slightly destabilizing conditions, and the B-chain region plays a critical role in the fibrillation. We show here that the isolated B-chain peptide of bovine insulin also forms fibrils at both acidic and neutral pH. When a mixture of insulin and the B-chain peptide was incubated at either acidic or neutral pH, the formation of fibrils was clearly separated into two phases, with the faster phase corresponding to the formation of homogeneous fibrils from the B-chain and the slower phase corresponding to homogeneous fibrillation of insulin. To further investigate the interaction (or lack thereof) between the two polypeptides, we examined the effects of cross-seeding. The results indicate that seeds of B-chain fibrils accelerate the fibrillation of insulin at pH 1.6 and inhibit the fibrillation at pH 7.5, but seeds of insulin fibrils have little effect on the fibrillation of the B-chain. We conclude that at pH 7.5 simultaneous independent homologous fibrillation occurs, but at low pH, heterologous fibrillation takes place, and with B-chain seeding of insulin, a unique conformation of fibrils is formed. Our results demonstrate that in the co-aggregation of closely related peptides each peptide species may undergo concurrent homogeneous or heterologous polymerization and that fibrils of one species may or may not seed fibrillation of the other. The results demonstrate the significant "species" barrier in amyloid fibril formation between fibrillation induced by different fibrils. A model for the fibrillation of the heterogeneous system of insulin and B-chain insulin is proposed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Hong DP,Fink ALdoi
10.1021/bi051658ysubject
Has Abstractpub_date
2005-12-20 00:00:00pages
16701-9issue
50eissn
0006-2960issn
1520-4995journal_volume
44pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00326a003
更新日期：1985-02-26 00:00:00
abstract：:Minibrain kinase/dual-specificity tyrosine phosphorylation regulated kinase 1A (MNB/DYRK1A) is a proline/arginine-directed serine/threonine kinase implicated in the learning deficits of Down syndrome. Epigallocatechin-3-gallate (EGCG), the major tea polyphenolic compound, is a potent MNB/DYRK1A inhibitor. In this stud...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060632j
更新日期：2006-10-03 00:00:00
abstract：:Specific intra- and intermolecular quadruplex conformations of model G-DNA oligonucleotides have been identified from their translational and rotational diffusion coefficients in aqueous solution. The transport properties were determined by polarized and depolarized dynamic light scattering. A comparison with hydrodyn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990750p
更新日期：1999-09-21 00:00:00
abstract：:The covalent affinity probe maltosyl isothiocyanate (MITC) has been used previously to identify the glucose transporter of human erythrocytes as a component of band 3. By use of limited proteolysis, the site on the Mr 100 000 protein to which MITC attaches has been localized to a 17 000-dalton region near the center o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00331a001
更新日期：1985-05-07 00:00:00
abstract：:In vivo bioluminescence imaging (BLI) has become a standard, non-invasive imaging modality for following gene expression or the fate of proteins and cells in living animals. Currently, bioluminescent reporters used in laboratories are mostly derivatives of two major luciferase families: ATP-dependent insect luciferase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01303
更新日期：2019-03-26 00:00:00
abstract：:A hallmark of penicillin-binding protein 2 (PBP2) from penicillin-resistant strains of Neisseria gonorrhoeae is insertion of an aspartate after position 345. The insertion resides on a loop near the active site and is immediately adjacent to an existing aspartate (Asp346) that forms a functionally important hydrogen b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5011317
更新日期：2014-12-09 00:00:00
abstract：:The exchangeability of apolipoprotein (apo) E between lipoprotein particles such as very low-density lipoprotein (VLDL) and high-density lipoprotein (HDL) is critical for lipoprotein metabolism, but despite its importance, the kinetics and mechanism of apoE-lipoprotein interaction are not known. We have used surface p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9000694
更新日期：2009-04-07 00:00:00
abstract：:High-resolution proton nuclear magnetic resonance (1H NMR) has been used to study the conformation and dynamics of biosynthetic analogues of actinomycin D that have been substituted at one or both of the 3'-amino acids with azetidine-2-carboxylic acid, pipecolic acid, or 4-ketoproline for one or both of the prolines. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00540a007
更新日期：1982-05-25 00:00:00
abstract：:The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971022+
更新日期：1997-09-30 00:00:00
abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00048a004
更新日期：1995-12-05 00:00:00
abstract：:The complete primary structure of the major component myoglobin from the Arctic minke whale, Balaenoptera acutorostrata, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Over 80% of the amino acid sequence was established from the three ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00623a023
更新日期：1977-02-22 00:00:00
abstract：:The systems of tRNA glycylation belong to the most complex aminoacylation systems since neither the oligomeric structure of glycyl-tRNA synthetases (GlyRS) nor the discriminator bases in tRNAGly are conserved in the phylae. To better understand the structure-function relationship in glycylation systems of various orig...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991392t
更新日期：1999-10-05 00:00:00
abstract：:An OxlT homology model suggests R272 and K355 in transmembrane helices 8 and 11, respectively, are critical to OxlT-mediated transport. We offer positive evidence supporting this idea by studying OxlT function after cysteine residues were separately introduced at these positions. Without further treatment, both mutant...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060746v
更新日期：2006-08-29 00:00:00
abstract：:Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025676g
更新日期：2002-06-04 00:00:00
abstract：:Acholeplasma laidlawii B cells, highly enriched in saturated C14 and C15 fatty acids, have been grown at several temperatures. Conformational order in the acyl chains of live cell membranes has been monitored with Fourier-transform infrared (FT-IR) spectroscopy. Coupled CH2 wagging progressions, characteristic of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00179a037
更新日期：1994-04-05 00:00:00
abstract：:Phenylalanine acts as an allosteric activator of the tetrahydropterin-dependent enzyme phenylalanine hydroxylase. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into local conformational changes accompanying activation of rat phenylalanine hydroxylase by phenylalanine. Peptide...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1001294
更新日期：2010-04-20 00:00:00
abstract：:We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951314f
更新日期：1996-03-05 00:00:00
abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991307h
更新日期：2000-03-14 00:00:00
abstract：:7-Ketocholesterol is an oxidized derivative of cholesterol with numerous physiological effects. In model membranes, 7-ketocholesterol and cholesterol were compared by physical measures of bilayer order and polarity, formation of detergent resistant domains (DRM), phase separation, and membrane microsolubilization by a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0506425
更新日期：2005-08-02 00:00:00
abstract：:Copper binding to the human copper chaperone for superoxide dismutase (hCCS) has been investigated by X-ray absorption spectroscopy. Stoichiometry measurements on the dialyzed, as-isolated protein indicated that up to 3.5 Cu ions bound per hCCS molecule. Reduction with either sodium dithionite or dithiothreitol decrea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000690j
更新日期：2000-06-27 00:00:00
abstract：:Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01156
更新日期：2018-03-06 00:00:00
abstract：:In globular proteins, there are intermolecular hydrogen bonds between protein and water molecules, and between water molecules, which are bound with the proteins, in addition to intramolecular hydrogen bonds. To estimate the contribution of these hydrogen bonds to the conformational stability of a protein, the thermod...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9910169
更新日期：1999-09-28 00:00:00
abstract：:The preparation, spectroscopic investigation, structure determination, conformational analysis, and modeling of the Dewar pyrimidinone photoproduct of thymidylyl-(3'----5')-thymidine, previously referred to as TpT3 [Johns, H. E., Pearson, M. L., LeBlanc, J. C., & Heilleiner, C. W. (1964) J. Mol. Biol. 9, 503-524], is ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00419a007
更新日期：1988-09-20 00:00:00
abstract：:There are highly conserved hydrogen bonds between the distal His and the adjacent Asn in many peroxidases. Although the crystal structure of horseradish peroxidase C (HRP) is not available, comparison of the amino acid sequence with cytochrome c peroxidase indicates that Asn70 is making the hydrogen bond with the dist...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961740g
更新日期：1996-11-12 00:00:00
abstract：:A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proxi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800659x
更新日期：2008-09-16 00:00:00
abstract：:Nogo-66 receptor (NgR1) is a leucine-rich repeat (LRR) protein that forms part of a signaling complex modulating axon regeneration. Previous studies have shown that the entire LRR region of NgR1, including the C-terminal cap of the LRR, LRRCT, is needed for ligand binding, and that the adjacent C-terminal region (CT s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0517483
更新日期：2005-12-20 00:00:00
abstract：:In bacteria, the transcription of virulence genes is usually controlled by a cell density-dependent process known as "quorum sensing" (QS). QS relies on small diffusible signaling molecules that cross the bacterial cell wall and activate target transcription factors after a threshold concentration has been reached. Be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900123j
更新日期：2009-11-03 00:00:00
abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980042o
更新日期：1998-07-14 00:00:00
abstract：:Crystal structures of complexes of a D30N mutant of feline immunodeficiency virus protease (FIV PR) complexed with a statine-based inhibitor (LP-149), as well as with a substrate based on a modification of this inhibitor (LP-149S), have been solved and refined at resolutions of 2.0 and 1.85 A, respectively. Both the i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9707436
更新日期：1997-09-02 00:00:00
abstract：:We have examined the binding of 125I-labeled human and mouse epidermal growth factors (EGF) to human alpha 2-macroglobulin (alpha 2M). In the presence of human neutrophil elastase, both mouse and human EGF bound to alpha 2M, whereas little binding was found to native alpha 2M. Binding was found to be predominantly cov...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a012
更新日期：1993-08-10 00:00:00
