Structural analysis of G-DNA in solution: A combination of polarized and depolarized dynamic light scattering with hydrodynamic model calculations.

abstract：:In an effort to further probe metal binding to metallo-beta-lactamase L1 (mbetal L1), Cu- (Cu-L1) and Ni-substituted (Ni-L1) L1 were prepared and characterized by kinetic and spectroscopic studies. Cu-L1 bound 1.7 equiv of Cu and small amounts of Zn(II) and Fe. The EPR spectrum of Cu-L1 exhibited two overlapping, axia...

journal_title：Biochemistry

authors： Hu Z,Spadafora LJ,Hajdin CE,Bennett B,Crowder MW

更新日期：2009-04-07 00:00:00

abstract：:The zinc-endopeptidase light chain of botulinum A neurotoxin undergoes autocatalytic fragmentation that is accelerated by the presence of the metal cofactor, zinc [Ahmed, S. A. et al. (2001) J. Protein Chem. 20, 221-231]. We show in this paper that >95% fragmented light chain obtained in the absence of added zinc reta...

journal_title：Biochemistry

authors： Ahmed SA,McPhie P,Smith LA

更新日期：2003-11-04 00:00:00

abstract：:NMR spectroscopy and simulated annealing calculations have been used to determine the three-dimensional structure of RK-1, an antimicrobial peptide from rabbit kidney recently discovered from homology screening based on the distinctive physicochemical properties of the corticostatins/defensins. RK-1 consists of 32 res...

journal_title：Biochemistry

authors： McManus AM,Dawson NF,Wade JD,Carrington LE,Winzor DJ,Craik DJ

更新日期：2000-12-26 00:00:00

abstract：:Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...

journal_title：Biochemistry

authors： Thielges MC,Chung JK,Axup JY,Fayer MD

更新日期：2011-06-28 00:00:00

abstract：:Disruption of the cell membrane by the β-amyloid (Aβ) peptides has been considered as a main mechanism of Alzheimer's disease. The peptide-to-lipid molar ratio (P:L) varies over a broad range biologically. We report here that two of the previously observed Aβ evolution pathways, fibrillation and induction of vesicle f...

journal_title：Biochemistry

authors： Akinlolu RD,Nam M,Qiang W

更新日期：2015-06-09 00:00:00

abstract：:The mechanism of herpesviral protease activation upon dimerization was studied using two independent spectroscopic assays augmented by directed mutagenesis. Spectroscopic changes, attributable to dimer interface conformational plasticity, were observed upon dimerization of Kaposi's sarcoma-associated herpesvirus prote...

journal_title：Biochemistry

authors： Pray TR,Reiling KK,Demirjian BG,Craik CS

更新日期：2002-02-05 00:00:00

abstract：:The glucose derivative, 2,2,6,6-tetramethylpiperidine-1-oxylglucose (TEMPO-glucose) was synthesized and examined for its ability to substitute for glucose as a substrate for the intestinal brush border membrane Na+/glucose cotransporter. TEMPO-glucose inhibited Na(+)-dependent phlorizin binding with an apparent KI of ...

journal_title：Biochemistry

authors： Peerce BE

更新日期：1991-04-30 00:00:00

abstract：:The role of the peripheral reaction center chlorophyll a molecule associated with His117 of the D2 polypeptide in photosystem II was investigated in Synechocystis sp. PCC 6803 using a combination of steady state, pump-probe, and picosecond time-resolved fluorescence spectroscopy. Data were obtained from intact cells a...

journal_title：Biochemistry

authors： Vasil'ev S,Bruce D

更新日期：2000-11-21 00:00:00

abstract：:NMR spectroscopic experiments were performed to study the structure of synthetic peptides identical to two extracellular regions of the human thrombin receptor. The smaller molecule, comprising 14 amino acids, was biologically active and was equivalent to the "tethered ligand" exposed after cleavage of the receptor by...

journal_title：Biochemistry

authors： Smith KJ,Trayer IP,Grand RJ

更新日期：1994-05-24 00:00:00

abstract：:Polymerase eta is a member of the Y family of DNA polymerases which is able to bypass thymine dimers efficiently and in a relatively error-free manner. To elucidate the mechanism of dimer bypass, the efficiency of dAMP and pyrene nucleotide insertion opposite the thymine dimer and its N3-methyl derivatives was determi...

journal_title：Biochemistry

authors： Sun L,Zhang K,Zhou L,Hohler P,Kool ET,Yuan F,Wang Z,Taylor JS

更新日期：2003-08-12 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:In iron-deficient conditions, Pseudomonas aeruginosa secretes a major fluorescent siderophore named pyoverdin (Pvd), which after chelating iron(III) is transported back into the cell via its outer membrane receptor FpvA. FpvA is a TonB-dependent transport protein and has the ability to bind Pvd in its apo- or iron-loa...

journal_title：Biochemistry

authors： Clément E,Mesini PJ,Pattus F,Schalk IJ

更新日期：2004-06-22 00:00:00

abstract：:The side-chain dynamics of methyl groups in two structurally related proteins from the fibronectin type III (fnIII) superfamily, the third fnIII domain from human tenascin (TNfn3) and the tenth fnIII domain from human fibronectin (FNfn10), have been studied by NMR spectroscopy. Side-chain order parameters reveal that ...

journal_title：Biochemistry

authors： Best RB,Rutherford TJ,Freund SM,Clarke J

更新日期：2004-02-10 00:00:00

abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

journal_title：Biochemistry

authors： Ruf A,de Murcia G,Schulz GE

更新日期：1998-03-17 00:00:00

abstract：:The fibronectin receptor (FnR), the vitronectin receptor (VnR), and the platelet membrane glycoprotein (GP) IIb-IIIa complex are members of a family of cell adhesion receptors, which consist of noncovalently associated alpha- and beta-subunits. The present study was designed to compare the cDNA-derived protein sequenc...

journal_title：Biochemistry

authors： Fitzgerald LA,Poncz M,Steiner B,Rall SC Jr,Bennett JS,Phillips DR

更新日期：1987-12-15 00:00:00

abstract：:A newly reported strain of Vibrio cholerae, known as strain O139 Bengal, is the first instance of an encapsulated strain that has caused epidemic cholera. The O-antigenic capsule is the critical antigen for protective immunity. Since mapping of the antigenic epitopes will assist in the development of a protein conjuga...

journal_title：Biochemistry

authors： Gunawardena S,Fiore CR,Johnson JA,Bush CA

更新日期：1999-09-14 00:00:00

abstract：:Lipidic membrane systems that have been reported to be composed of sphingomyelin (SM)-cholesterol (Chol) microdomains or "rafts" by Dietrich et al. [palmitoyloleoyl-phosphatidylcholine(POPC)/SM/Chol, 1/1/1; Dietrich, C., Bagatolli, L. A., Volovyk, Z. N., Thompson, N. L., Levi, M., Jacobson, K., and Gratton, E. (2001) ...

journal_title：Biochemistry

authors： Aussenac F,Tavares M,Dufourc EJ

更新日期：2003-02-18 00:00:00

abstract：:Two thiol-activated endopeptidases with pH optima near pH 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. ...

journal_title：Biochemistry

authors： Oliveira EB,Martins AR,Camargo AC

更新日期：1976-05-04 00:00:00

abstract：:Ppr from the purple phototrophic bacterium, Rhodospirillum centenum (also known as Rhodocista centenaria), is a hybrid of photoactive yellow protein (PYP), bacteriophytochrome (Bph), and histidine kinase (HK) domains. The holo-Ppr (containing both chromophores) exhibits characteristic absorption maxima at 435 nm due t...

journal_title：Biochemistry

authors： Kyndt JA,Fitch JC,Meyer TE,Cusanovich MA

更新日期：2007-07-17 00:00:00

abstract：:In a previous report, the cDNA for human proteinase inhibitor 8 (PI8) was first identified, isolated, and subcloned into a mammalian expression vector and expressed in baby hamster kidney cells. Initial studies indicated that PI8 was able to inhibit the amidolytic activity of trypsin and form an SDS-stable approximate...

journal_title：Biochemistry

authors： Dahlen JR,Foster DC,Kisiel W

更新日期：1997-12-02 00:00:00

abstract：:The malaria parasite pigment, hemozoin, is a crystal of ferriprotoporphyrin IX (FP-Fe(III)), a product of hemoglobin digestion. Hemozoin formation is essential for FP-Fe(III) detoxification in the parasite; it is the main target of quinoline antimalarials and can modulate immune and inflammation responses. To gain fur...

journal_title：Biochemistry

authors： Klonis N,Dilanian R,Hanssen E,Darmanin C,Streltsov V,Deed S,Quiney H,Tilley L

更新日期：2010-08-10 00:00:00

abstract：:The binding of formate ion, a substrate for the peroxidatic reaction of catalase, has been investigated by magnetic resonance techniques. Comparative studies of formate binding to ferric myoglobin have also been performed. The nuclear magnetic relaxation (NMR) rate of formate and water protons is enhanced by the prese...

journal_title：Biochemistry

authors： Hershberg RD,Chance B

更新日期：1975-08-26 00:00:00

abstract：:Conformational alterations occurring in bovine alpha 2-macroglobulin (alpha 2M) resulting from proteolysis and nucleophilic modification have been monitored by UV difference spectra, circular dichroism, and changes in the fluorescence of 6-(p-toluidino)-2-naphthalenesulfonate (TNS) and bis(8-anilino-1-naphthalenesulfo...

journal_title：Biochemistry

authors： Strickland DK,Steiner JP,Feldman SR,Pizzo SV

更新日期：1984-12-18 00:00:00

abstract：:The solution structure of the self-complementary dodecamer 5'd(CGCGPATTCGCG)2, containing a purine-thymine base pair within the hexameric canonical recognition site GAATTC for the restriction endonuclease EcoRI, is investigated by nuclear magnetic resonance spectroscopy and restrained molecular dynamics. Nonexchangeab...

journal_title：Biochemistry

authors： Clore GM,Oschkinat H,McLaughlin LW,Benseler F,Happ CS,Happ E,Gronenborn AM

更新日期：1988-05-31 00:00:00

abstract：:Steady-state and femtosecond time-resolved optical methods have been used to compare the spectroscopic features and energy transfer dynamics of two systematically different light-harvesting complexes from the dinoflagellate Amphidinium carterae: main-form (MFPCP) and high-salt (HSPCP) peridinin-chlorophyll a-proteins....

journal_title：Biochemistry

authors： Ilagan RP,Koscielecki JF,Hiller RG,Sharples FP,Gibson GN,Birge RR,Frank HA

更新日期：2006-11-28 00:00:00

abstract：:A critical event in Alzheimer's disease is the transition of Abeta peptides from their soluble forms into disease-associated beta-sheet-rich conformers. Structural analysis of a complete D-amino acid replacement set of Abeta(1-42) enabled us to localize in the full-length 42-mer peptide the region responsible for the ...

journal_title：Biochemistry

authors： Janek K,Rothemund S,Gast K,Beyermann M,Zipper J,Fabian H,Bienert M,Krause E

更新日期：2001-05-08 00:00:00

abstract：:ATP-dependent Mg2+ accumulation in isolated mitochondria occurs predominantly in the matrix and inner membrane compartments. In mitochondria contaminated with lysosomes, the time course and magnitude of ATP-dependent Mg2+ accumulation are influenced by various cytoplasmic substances, besides substrates of the citric a...

journal_title：Biochemistry

authors： Kun E

更新日期：1976-06-01 00:00:00

abstract：:An NMR and nuclear Overhauser effect (NOE) analysis of Thermus thermophilus tRNAIle1a is presented. This species contains modifications including s2T54 and s4U8 [Horie, N., Hara-Yokoyama, M., Yokoyama, S., Watanabe, K., Kuchino, Y., Nishimura, S., & Miyazawa, T. (1985) Biochemistry 24, 5711-5715]. All the expected sec...

journal_title：Biochemistry

authors： Choi BS,Redfield AG

更新日期：1986-04-08 00:00:00

abstract：:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(II...

journal_title：Biochemistry

authors： Rich AM,Ellis PJ,Tennant L,Wright PE,Armstrong RS,Lay PA

更新日期：1999-12-14 00:00:00

abstract：:Human apolipoprotein(a) kringle IV type 10 [apo(a) KIV(10)] contains a strong lysine-binding site (LBS) that mediates the interaction of Lp(a) with biological substrates such as fibrin. Mutations in the KIV(10) LBS have been reported in both the rhesus monkey and chimpanzee, and have been proposed to explain the lack ...

journal_title：Biochemistry

authors： Belczewski AR,Ho J,Taylor FB Jr,Boffa MB,Jia Z,Koschinsky ML

更新日期：2005-01-18 00:00:00