Study of the conformational transition of A beta(1-42) using D-amino acid replacement analogues.

abstract：:The mechanism of chromophore biosynthesis in green fluorescent protein (GFP) is triggered by a spontaneous main chain cyclization reaction of residues 65-67. Here, we demonstrate that the initially colorless Y66L variant, designed to trap chromophore precursor states, is oxidatively modified to generate yellow chromop...

journal_title：Biochemistry

authors： Rosenow MA,Patel HN,Wachter RM

更新日期：2005-06-14 00:00:00

abstract：:An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...

journal_title：Biochemistry

authors： Klein C,Hollender J,Bender H,Schulz GE

更新日期：1992-09-22 00:00:00

abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...

journal_title：Biochemistry

authors： Komives EA,Lougheed JC,Liu K,Sugio S,Zhang Z,Petsko GA,Ringe D

更新日期：1995-10-17 00:00:00

abstract：:Epitranscriptomic RNA modifications can serve as recognition elements for the recruitment of effector proteins (i.e., "readers") to modified transcripts. While these interactions play an important role in mRNA regulation, there is a major gap in our understanding of the sequence determinants critical for the binding o...

journal_title：Biochemistry

authors： Arguello AE,Leach RW,Kleiner RE

更新日期：2019-08-06 00:00:00

abstract：:A strategy to design potent antagonists of human coagulation factor VIIa (FVIIa) by linking two proteins that independently inhibit activity and bind at separate, nonoverlapping sites is presented. A bifunctional inhibitor (KDTF5), comprising a Kunitz-type domain engineered to inhibit the FVIIa active site and a solub...

journal_title：Biochemistry

authors： Lee GF,Lazarus RA,Kelley RF

更新日期：1997-05-13 00:00:00

abstract：:Ks values for binding of selected substrates, competitive inhibitors, and a noncompetitive inhibitor were found to be similar for alpha-chymotrypsin and N-methyl-alpha-chymotrypsin. The rates and steps of binding of a competitive inhibitor and a noncompetitive inhibitor were also found to be similar for alpha-chymotry...

journal_title：Biochemistry

authors： Maehler R,Whitaker JR

更新日期：1982-09-14 00:00:00

abstract：:Nitrogenase catalyzes the reduction of N2 to NH3, supporting all biological nitrogen fixation. Electron donors to this enzyme are ferredoxin or flavodoxin (in vivo) and sodium dithionite (in vitro). Features of these electron donors put a limit on spectrophotometric studies and electrocatalytic applications of nitroge...

journal_title：Biochemistry

authors： Badalyan A,Yang ZY,Hu B,Luo J,Hu M,Liu TL,Seefeldt LC

更新日期：2019-11-19 00:00:00

abstract：:Glycosaminoglycans (GAGs) have been reported to play a significant role in amyloid formation of a wide range of proteins/peptides either associated with diseases or native biological functions. The exact mechanism by which GAGs influence amyloid formation is not clearly understood. Here, we studied two closely related...

journal_title：Biochemistry

authors： Jha NN,Anoop A,Ranganathan S,Mohite GM,Padinhateeri R,Maji SK

更新日期：2013-12-10 00:00:00

abstract：:The nontransformed steroid receptors contain several non-steroid binding proteins, such as hsp90, hsp70, and p59. Recently, we and others have shown that p59 (FKBP59) is an immunophilin which binds two potent immunosuppressants, FK506 and rapamycin. This raises the possibility that FK506 or rapamycin may modify the fu...

journal_title：Biochemistry

authors： Tai PK,Albers MW,McDonnell DP,Chang H,Schreiber SL,Faber LE

更新日期：1994-09-06 00:00:00

abstract：:The physicochemical and catalytic properties of thioredoxin-T' are described. This complemented protein structure consists of a 1:1 complex between the inactive fragments thioredoxin-T-(1--73) and thioredoxin T-(74--108). These are generated by selective trypsin cleavage at Arg-73 in lysine-modified and denatured Esch...

journal_title：Biochemistry

authors： Slabý I,Holmgren A

更新日期：1979-12-11 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:Single-tryptophan-containing mutants of low adenylation state Escherichia coli glutamine synthetase (wild type has two tryptophans at positions 57 and 158) have been constructed and studied by multifrequency phase/modulation fluorescence spectroscopy. The W57L mutant (retains tryptophan at residue 158) and the W158S m...

journal_title：Biochemistry

authors： Atkins WM,Stayton PS,Villafranca JJ

更新日期：1991-04-09 00:00:00

abstract：:The conserved glycines in the glycine-rich loop (Leu-Gly50-Thr-Gly52-Ser-Phe-Gly55-Arg-Val) of the catalytic (C) subunit of cAMP-dependent protein kinase were each mutated to Ser (G50S, G52S, and G55S). The effects of these mutations were assessed here using both steady-state and pre-steady-state kinetic methods. Whil...

journal_title：Biochemistry

authors： Grant BD,Hemmer W,Tsigelny I,Adams JA,Taylor SS

更新日期：1998-05-26 00:00:00

abstract：:Lateral mobilities of fluorescent cell surface probes have been measured on normal (3T3) and transformed (SV3T3) cultured mouse fibroblasts. There is little discernible difference in the mobilities of a lipid analogue (diI), a fluorescent ganglioside derivative (GM1), and tetramethylrhodamine-labeled succinylated conc...

journal_title：Biochemistry

authors： Eldridge CA,Elson EL,Webb WW

更新日期：1980-05-13 00:00:00

abstract：:An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...

journal_title：Biochemistry

authors： Cabezas A,Pinto RM,Fraiz F,Canales J,González-Santiago S,Cameselle JC

更新日期：2001-11-13 00:00:00

abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

journal_title：Biochemistry

authors： Kuhlmann J,Macara I,Wittinghofer A

更新日期：1997-10-07 00:00:00

abstract：:To better understand the factors controlling the binding of apolipoprotein molecules at the surfaces of serum lipoprotein particles, the adsorption of human apolipoprotein A-I to phospholipid monolayers has been studied. The influence of lipid packing was investigated by spreading the monolayers at various initial sur...

journal_title：Biochemistry

authors： Ibdah JA,Phillips MC

更新日期：1988-09-06 00:00:00

abstract：:A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...

journal_title：Biochemistry

authors： Sancho J,Serrano L,Fersht AR

更新日期：1992-03-03 00:00:00

abstract：:This report presents evidence for interdomain steps of the ligand-coupled signal transduction mechanism of the oxygen receptor from Rhizobium meliloti, RmFixL. Photolysis of the CO adducts of heme domain (RmFixLN) and heme kinase (RmFixL*) proteins allowed tracking of second-order heme CO recombination reactions by tr...

journal_title：Biochemistry

authors： Rodgers KR,Tang L,Lukat-Rodgers GS,Wengenack NL

更新日期：2001-10-30 00:00:00

abstract：:The initial step in the l-kynurenine pathway is oxidation of l-tryptophan to N-formylkynurenine and is catalyzed by one of two heme enzymes, tryptophan 2,3-dioxygenase (TDO) or indoleamine 2,3-dioxygenase (IDO). Here, we address the role of the conserved active site Ser167 residue in human IDO (S167A and S167H variant...

journal_title：Biochemistry

authors： Chauhan N,Basran J,Efimov I,Svistunenko DA,Seward HE,Moody PC,Raven EL

更新日期：2008-04-22 00:00:00

abstract：:Most duplex DNAs that are in the "B" conformation are not immunogenic. One important exception is poly(dG) X poly(dC), which produces a good immune response even though, by many criteria, it adopts a conventional right-handed helix. In order to investigate what features are being recognized, monoclonal antibodies were...

journal_title：Biochemistry

authors： Lee JS,Woodsworth ML,Latimer LJ

更新日期：1984-07-03 00:00:00

abstract：:The light harvesting 1 antenna (LH1) complex from Rhodobacter sphaeroides funnels excitation energy to the photosynthetic reaction center. Our ultimate goal is to build up the structure of LH1 from structures of its individual subunits, much as the antenna can self-assemble from its components in membrane-mimicking de...

journal_title：Biochemistry

authors： Sorgen PL,Cahill SM,Krueger-Koplin RD,Krueger-Koplin ST,Schenck CC,Girvin ME

更新日期：2002-01-08 00:00:00

abstract：:Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...

journal_title：Biochemistry

authors： Kobayashi K,Koppenhöfer A,Ferguson SJ,Tagawa S

更新日期：1997-11-04 00:00:00

abstract：:We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...

journal_title：Biochemistry

authors： Godson GN,Mustaev AA,Sun W

更新日期：1998-03-17 00:00:00

abstract：:5-Hydroxy-1,2-naphthalenedicarboxylic anhydride is closely related to its precursor dibasic acid which is a metabolite of the carcinogenic polynuclear hydrocarbon dibenz[a,h]anthracene. The anhydride inhibited respiration of coupled mitochondria. This inhibition was relieved by 2,4-dinitrophenol. Several mitochondrial...

journal_title：Biochemistry

authors： Hadler HI,Demetriou JM

更新日期：1975-12-02 00:00:00

abstract：:Plexins are transmembrane proteins that serve as guidance receptors during angiogenesis, lymphangiogenesis, neuronal development, and zebrafish fin regeneration, with a putative role in cancer metastasis. Receptor dimerization or clustering, induced by extracellular ligand binding but modulated in part by the plexin t...

journal_title：Biochemistry

authors： Barton R,Khakbaz P,Bera I,Klauda JB,Iovine MK,Berger BW

更新日期：2016-09-06 00:00:00

abstract：:The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...

journal_title：Biochemistry

authors： Berdis AJ,Benkovic SJ

更新日期：1997-03-11 00:00:00

abstract：:Two synthetic peptides corresponding to sequences in HIV-1LAI gp41, (aa558-595) and T20 (aa 643-678), are strong inhibitors of HIV-1 viral fusion, having EC50 values of 1 microgram/mL and 1 ng/mL, respectively. Previous work suggested that T21 forms a coiled-coil structure in PBS solution, while T20 is primarily nonhe...

journal_title：Biochemistry

authors： Lawless MK,Barney S,Guthrie KI,Bucy TB,Petteway SR Jr,Merutka G

更新日期：1996-10-22 00:00:00

abstract：:The interaction of fluorescent ATP analog 2'(3')-O-[N-[2-[3-(5-fluoresceinyl)thioureido]-ethyl]carbamoyl]adenosine 5'-triphosphate (FEDA-ATP) with rabbit skeletal myosin subfragment 1 (S1) and acto-S1 was studied. This and related ATP analogs are potentially useful for determination of the ATPase activity of single my...

journal_title：Biochemistry

authors： Conibear PB,Jeffreys DS,Seehra CK,Eaton RJ,Bagshaw CR

更新日期：1996-02-20 00:00:00

abstract：:The traditional approach to studying protein folding involves applying a perturbation, usually denaturant or mutation, and determining the effect upon the free energy of folding, DeltaG0, and the activation free energy, DeltaG(not equal). Data collected as a function of the perturbation can be used to construct rate e...

journal_title：Biochemistry

authors： Song B,Cho JH,Raleigh DP

更新日期：2007-12-11 00:00:00