Study of the conformational transition of A beta(1-42) using D-amino acid replacement analogues.

abstract：:An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...

journal_title：Biochemistry

authors： Klein C,Hollender J,Bender H,Schulz GE

更新日期：1992-09-22 00:00:00

abstract：:The effect of opportunistic infections (OI) on immune-compromised populations has been known for decades, but the recent AIDS epidemic has sparked renewed interest in the development of new anti-OI agents. The mechanism of action of a series of cationic unfused-aromatic anti-OI drugs is believed to involve binding of ...

journal_title：Biochemistry

authors： Tanious FA,Ding D,Patrick DA,Tidwell RR,Wilson WD

更新日期：1997-12-09 00:00:00

abstract：:The respiratory chain in the cytochrome d deficient mutant Escherichia coli GR19N is a relatively simple, linear system consisting of primary dehydrogenases, ubiquinone 8, cytochrome b-556, and cytochrome o oxidase. By use of right-side-out and inside-out membrane vesicles from this strain, various oxidase activities ...

journal_title：Biochemistry

authors： Matsushita K,Kaback HR

更新日期：1986-05-06 00:00:00

abstract：:Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...

journal_title：Biochemistry

authors： Wadkins RM,Graves DE

更新日期：1991-04-30 00:00:00

abstract：:We measured the time course of skeletal muscle actin polymerization at different actin concentrations. In 0.1 M KCl with 1 mM Mg2+, log/log plots of the rate of the early, slow phase of polymerization vs. actin concentration were linear with slopes from 1.0 to 1.3. Computer-assisted calculations of similar curves from...

journal_title：Biochemistry

authors： Cooper JA,Buhle EL Jr,Walker SB,Tsong TY,Pollard TD

更新日期：1983-04-26 00:00:00

abstract：:Miniproteins reduce the complexity of the protein-folding problem allowing systematic studies of contributions to protein folding and stabilization. Here, we describe the rational redesign of a miniprotein, PPα, comprising a polyproline II helix, a loop, and an α helix. The redesign provides a de novo framework for in...

journal_title：Biochemistry

authors： Porter Goff KL,Nicol D,Williams C,Crump MP,Zieleniewski F,Samphire JL,Baker EG,Woolfson DN

更新日期：2019-07-16 00:00:00

abstract：:Fluorescent derivatives of short oligonucleotides of defined sequence were prepared by the incorporation of 5-(propylamino)uridine via current phosphoramidite chemistry, followed by derivatization of the propylamine function with mansyl chloride. These oligomers, annealed to complementary oligomers, yielded short dupl...

journal_title：Biochemistry

authors： Allen DJ,Darke PL,Benkovic SJ

更新日期：1989-05-30 00:00:00

abstract：:The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...

journal_title：Biochemistry

authors： Reiling KK,Endres NF,Dauber DS,Craik CS,Stroud RM

更新日期：2002-04-09 00:00:00

abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...

journal_title：Biochemistry

authors： Miron S,Durand D,Chilom C,Pérez J,Craescu CT

更新日期：2011-07-26 00:00:00

abstract：:Klebsiella aerogenes urease uses a dinuclear nickel active site to catalyze the hydrolysis of urea. Here, we describe the steady-state and pre-steady-state kinetics of urease inhibition by fluoride. Urease is slowly inhibited by fluoride in both the presence and absence of substrate. Steady-state rate studies yield pa...

journal_title：Biochemistry

authors： Todd MJ,Hausinger RP

更新日期：2000-05-09 00:00:00

abstract：:Raman spectroscopy was used to study the phase behavior of several semisynthetic species of the acidic glycosphingolipid cerebroside sulfate (CBS) which occur in myelin. The C-H stretching mode region at 2800-3100 cm-1 of C18:0-CBS, C24:0-CBS, and C26:0-CBS, and the alpha-hydroxy fatty acid species C18:0h-CBS, was stu...

journal_title：Biochemistry

authors： Stevenson CC,Rich NH,Boggs JM

更新日期：1992-02-18 00:00:00

abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

journal_title：Biochemistry

authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

更新日期：1994-09-20 00:00:00

abstract：:Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...

journal_title：Biochemistry

authors： Longo A,Guanga GP,Rose RB

更新日期：2007-03-20 00:00:00

abstract：:The nitrogenase MoFe protein contains the active site metallocluster called FeMo-cofactor [7Fe-9S-Mo-homocitrate] that exhibits an S = 3/2 EPR signal in the resting state. No interaction with FeMo-cofactor is detected when either substrates or inhibitors are incubated with MoFe protein in the resting state. Rather, th...

journal_title：Biochemistry

authors： Benton PM,Mayer SM,Shao J,Hoffman BM,Dean DR,Seefeldt LC

更新日期：2001-11-20 00:00:00

abstract：:The AT-rich interaction domain (ARID) containing BAF250a is a subunit of the BAF-A class of SWI/SNF chromatin remodeling complexes. The ARID belongs to a family of conserved DNA binding domains found in several eukaryotic proteins; however, its exact contribution to BAF250a function and the mechanism of its DNA bindin...

journal_title：Biochemistry

authors： Giri M,Maulik A,Singh M

更新日期：2020-01-14 00:00:00

abstract：:The murine urokinase-type plasminogen activator (uPA) gene has been isolated from a BALB/c liver DNA cosmid library and its nucleotide sequence established. The gene is organized into 11 exons comprising 34.7% of the 6710 base pair (bp) region spanning the interval between the presumed transcription initiation and pol...

journal_title：Biochemistry

authors： Degen SJ,Heckel JL,Reich E,Degen JL

更新日期：1987-12-15 00:00:00

abstract：:A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...

journal_title：Biochemistry

authors： Pryor C,Bridge M,Loew LM

更新日期：1985-04-23 00:00:00

abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

journal_title：Biochemistry

authors： Ruf A,de Murcia G,Schulz GE

更新日期：1998-03-17 00:00:00

abstract：:The acid-base properties of ionophore A23187 in methanol-water solutions (0--95% w/w) and bound to unilamellar vesicles of dimyristoylphosphatidylcholine were examined by ultraviolet and fluorescence spectroscopy, and the spectral properties for the acidic and basic forms were defined under these conditions. Standard ...

journal_title：Biochemistry

authors： Kauffman RF,Taylor RW,Pfeiffer DR

更新日期：1982-05-11 00:00:00

abstract：:Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without compromising its ability to amplify ...

journal_title：Biochemistry

authors： Wu EY,Walsh AR,Materne EC,Hiltner EP,Zielinski B,Miller BR 3rd,Mawby L,Modeste E,Parish CA,Barnes WM,Kermekchiev MB

更新日期：2015-01-27 00:00:00

abstract：:Using the baculovirus/Sf9 cell expression system, we have incorporated 99% 15N-enriched [alpha,epsilon-15N2]-L-lysine into the rod visual pigment rhodopsin. We have subsequently investigated the protonated Schiff base (pSB) linkage in the [alpha, epsilon-15N2]Lys-rhodopsin with cross-polarization magic angle spinning ...

journal_title：Biochemistry

authors： Creemers AF,Klaassen CH,Bovee-Geurts PH,Kelle R,Kragl U,Raap J,de Grip WJ,Lugtenburg J,de Groot HJ

更新日期：1999-06-01 00:00:00

abstract：:The human intestinal Caco-2 cell line, described as enterocyte-like in a number of studies, was examined for its ability to carry out the metabolism of vitamin A normally required in the absorptive process. Caco-2 cells contained cellular retinol-binding protein II, a protein which is abundant in human villus-associat...

journal_title：Biochemistry

authors： Quick TC,Ong DE

更新日期：1990-12-18 00:00:00

abstract：:The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...

journal_title：Biochemistry

authors： Lin SH,Lee JC

更新日期：2002-10-01 00:00:00

abstract：:The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase ...

journal_title：Biochemistry

authors： Carlson GM,Graves DJ

更新日期：1976-10-05 00:00:00

abstract：:l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

journal_title：Biochemistry

authors： Chow C,Hegde S,Blanchard JS

更新日期：2017-08-08 00:00:00

abstract：:The mechanism of inactivation of human leukocyte elastase (HLE) by the chloromethyl ketone MeOSuc-Ala-Ala-Pro-Val-CH2Cl was investigated. The dependence of the first-order rate constant for inactivation on concentration of chloromethyl ketone is hyperbolic and suggests formation of a reversible "Michaelis complex" pri...

journal_title：Biochemistry

authors： Stein RL,Trainor DA

更新日期：1986-09-23 00:00:00

abstract：:Previous studies of P-glycoprotein have demonstrated that its function can be modulated by phosphorylation. In the present study, inhibition of protein kinase C with calphostin C or stauroporine or prolonged treatment with the phorbol ester TPA decreased phosphorylation of P-glycoprotein, and impaired transport of vin...

journal_title：Biochemistry

authors： Bates SE,Lee JS,Dickstein B,Spolyar M,Fojo AT

更新日期：1993-09-07 00:00:00

abstract：:A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...

journal_title：Biochemistry

authors： Giovannangeli C,Perrouault L,Escudé C,Nguyen T,Hélène C

更新日期：1996-08-13 00:00:00

abstract：:The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...

journal_title：Biochemistry

authors： Colussi T,Parsonage D,Boles W,Matsuoka T,Mallett TC,Karplus PA,Claiborne A

更新日期：2008-01-22 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR) is a plasmid-encoded, type II enzyme. Four monomers (78 amino acids long) assemble into a homotetramer possessing 222 symmetry. In previous studies, a tandem array of four R67 DHFR gene copies was fused in frame to generate a functional monomer named Quad1. This protein possessed the...

journal_title：Biochemistry

authors： Feng J,Grubbs J,Dave A,Goswami S,Horner CG,Howell EE

更新日期：2010-08-31 00:00:00