当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Kinetic evidence for a monomer activation step in actin polymerization.

Kinetic evidence for a monomer activation step in actin polymerization.

Abstract：

:We measured the time course of skeletal muscle actin polymerization at different actin concentrations. In 0.1 M KCl with 1 mM Mg2+, log/log plots of the rate of the early, slow phase of polymerization vs. actin concentration were linear with slopes from 1.0 to 1.3. Computer-assisted calculations of similar curves from theoretical models with different sizes for the nucleus showed that no simple model gave a log/log plot with a slope less than 1.5. Addition of a first-order, monomer activation step before nucleation allowed models of any reasonable nucleus size to have a slope of 1. This is the first evidence that such a step is part of the kinetic pathway for actin polymerization. In 0.1 M KCl with 0.2 mM Ca2+, log/log plots of the rate of the slow phase vs. actin concentration were linear with slopes from 2.0 to 2.5. Monomer activation was not necessary to account for this slope. However, fits of kinetic curves calculated from theoretical models to experimental kinetic curves showed that filament fragmentation was important to achieve a good fit, confirming the finding of Wegner and Savko [Wegner, A., & Savko, P. (1982) Biochemistry 21, 1909-1913]. Our fit procedure also allowed us to estimate the size of the nucleus and the rate constants for activation, nucleation, and fragmentation. In 0.1 M KCl with 1 mM Mg2+, the nucleus was a dimer or trimer, and nucleation was fast. In 0.1 M KCl with 2.0 mM Ca2+, the nucleus was a trimer, and nucleation was slow.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Cooper JA,Buhle EL Jr,Walker SB,Tsong TY,Pollard TD

doi

10.1021/bi00278a021

subject

Has Abstract

pub_date

1983-04-26 00:00:00

pages

2193-202

issue

9

eissn

0006-2960

issn

1520-4995

journal_volume

22

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Involvement of insulin-like growth factor type 1 receptor and protein kinase Cdelta in bis(maltolato)oxovanadium(IV)-induced phosphorylation of protein kinase B in HepG2 cells.

    abstract：:Vanadium(IV) oxo-bis(maltolato) (BMOV), an organovanadium compound, is a potent insulinomimetic agent and improves glucose homeostasis in various models of diabetes. We have shown previously that BMOV stimulates the phosphorylation of PKB which may contribute as one of the mechanisms for the insulinomimetic effect of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060403x

    authors： Mehdi MZ,Vardatsikos G,Pandey SK,Srivastava AK

    更新日期：2006-09-26 00:00:00

  • Poly(pro)II helices in globular proteins: identification and circular dichroic analysis.

    abstract：:A method to identify poly(L-proline)-type (PII) conformation in crystal structures of globular proteins is presented. Short segments of PII structure were identified in globular protein structures, and these form a significant fraction of the residues which are not assigned to alpha-helix, beta-sheet, and beta-turns. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a028

    authors： Sreerama N,Woody RW

    更新日期：1994-08-23 00:00:00

  • Effects of pH and iminosugar pharmacological chaperones on lysosomal glycosidase structure and stability.

    abstract：:Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9002265

    authors： Lieberman RL,D'aquino JA,Ringe D,Petsko GA

    更新日期：2009-06-09 00:00:00

  • Sequence-specific solution structure of d-GGTACGCGTACC.

    abstract：:Complete resonance assignments of nonexchangeable base protons and sugar protons in d-GGTACGCGTACC at 500 MHz have been obtained by two-dimensional correlated spectroscopy (COSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The characteristic phase-sensitive multiplet patterns of the ntrasugar cross peaks ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00410a050

    authors： Chary KV,Hosur RV,Govil G,Chen CQ,Miles HT

    更新日期：1988-05-17 00:00:00

  • Tyr51: Key Determinant of the Low Thermostability of the Colwellia psychrerythraea Cold-Shock Protein.

    abstract：:Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile Colwellia psychrerythraea 34H, ( Cp-Csp). Althou...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00144

    authors： Lee Y,Kwak C,Jeong KW,Durai P,Ryu KS,Kim EH,Cheong C,Ahn HC,Kim HJ,Kim Y

    更新日期：2018-07-03 00:00:00

  • Substrate binding in the FAD-dependent hydroxynitrile lyase from almond provides insight into the mechanism of cyanohydrin formation and explains the absence of dehydrogenation activity.

    abstract：:In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802162s

    authors： Dreveny I,Andryushkova AS,Glieder A,Gruber K,Kratky C

    更新日期：2009-04-21 00:00:00

  • Localization in the Golgi apparatus of rat liver UDP-Gal:glucosylceramide beta 1----4galactosyltransferase.

    abstract：:The presence and subcellular localization of UDP-Gal:glucosylceramide beta 1----4galactosyltransferase (GalT-2) was investigated in rat liver. For this purpose, purified Golgi apparatus, endoplasmic reticulum, and plasma membrane fractions were prepared from the liver and used as the enzyme source for detecting GalT-2...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00224a021

    authors： Trinchera M,Fiorilli A,Ghidoni R

    更新日期：1991-03-12 00:00:00

  • Preferred conformation and dynamics of the glycerol backbone in phospholipids. An NMR and X-ray single-crystal analysis.

    abstract：:The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00426a014

    authors： Hauser H,Pascher I,Sundell S

    更新日期：1988-12-27 00:00:00

  • N-terminal disordered domain of Saccharomyces cerevisiae Hop1 protein is dispensable for DNA binding, bridging, and synapsis of double-stranded DNA molecules but is necessary for spore formation.

    abstract：:The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4005528

    authors： Khan K,Madhavan TP,Kshirsagar R,Boosi KN,Sadhale P,Muniyappa K

    更新日期：2013-08-06 00:00:00

  • Cholesterol-mediated conformational changes in the steroidogenic acute regulatory protein are essential for steroidogenesis.

    abstract：:Although the mechanism by which the steroidogenic acute regulatory protein (StAR) promotes steroidogenesis has been studied extensively, it remains incompletely characterized. Because structural analysis has revealed a hydrophobic sterol-binding pocket (SBP) within StAR, this study sought to examine the regulatory rol...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401125v

    authors： Rajapaksha M,Kaur J,Bose M,Whittal RM,Bose HS

    更新日期：2013-10-15 00:00:00

  • The structures of the C185S and C185A mutants of sulfite oxidase reveal rearrangement of the active site.

    abstract：:Sulfite oxidase (SO) catalyzes the physiologically critical conversion of sulfite to sulfate. Enzymatic activity is dependent on the presence of the metal molybdenum complexed with a pyranopterin-dithiolene cofactor termed molybdopterin. Comparison of the amino acid sequences of SOs from a variety of sources has ident...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1001954

    authors： Qiu JA,Wilson HL,Pushie MJ,Kisker C,George GN,Rajagopalan KV

    更新日期：2010-05-11 00:00:00

  • Delta-elimination by T4 endonuclease V at a thymine dimer site requires a secondary binding event and amino acid Glu-23.

    abstract：:Endonuclease V from bacteriophage T4 is a well characterized enzyme that initiates the repair of ultraviolet light induced pyrimidine dimers. Scission of the phosphodiester backbone between the pyrimidines within a dimer, or 3' to an abasic (AP) site, occurs by a beta-elimination mechanism. In addition, high concentra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00027a031

    authors： Latham KA,Lloyd RS

    更新日期：1995-07-11 00:00:00

  • Bilayer interactions of ether- and ester-linked phospholipids: dihexadecyl- and dipalmitoylphosphatidylcholines.

    abstract：:Mixed phospholipid systems of ether-linked 1,2-dihexadecylphosphatidylcholine (DHPC) and ester-linked 1,2-dipalmitoylphosphatidylcholine (DPPC) have been studied by differential scanning calorimetry and X-ray diffraction. At maximum hydration (60 wt % water), DHPC shows three reversible transitions: a main (chain melt...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00395a006

    authors： Kim JT,Mattai J,Shipley GG

    更新日期：1987-10-20 00:00:00

  • Structural analysis of the PsbQ protein of photosystem II by Fourier transform infrared and circular dichroic spectroscopy and by bioinformatic methods.

    abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026575l

    authors： Balsera M,Arellano JB,Gutiérrez JR,Heredia P,Revuelta JL,De Las Rivas J

    更新日期：2003-02-04 00:00:00

  • Chloroplast biogenesis: [4-vinyl] chlorophyllide a reductase is a divinyl chlorophyllide a-specific, NADPH-dependent enzyme.

    abstract：:Some properties of [4-vinyl] chlorophyllide a reductase are described. This enzyme converts divinyl chlorophyllide a to monovinyl chlorophyllide a. The latter is the immediate precursor of monovinyl chlorophyll a, the main chlorophyll in green plants. [4-Vinyl] chlorophyllide a reductase plays an important role in day...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00151a011

    authors： Parham R,Rebeiz CA

    更新日期：1992-09-15 00:00:00

  • Electronic absorption, EPR, and resonance raman spectroscopy of CooA, a CO-sensing transcription activator from R. rubrum, reveals a five-coordinate NO-heme.

    abstract：:Electronic absorption, EPR, and resonance Raman spectroscopies revealed that CooA, the CO-sensing transcriptional regulator from Rhodospirillum rubrum, reacts with NO to form a five-coordinate NO-heme. NO must therefore displace both of the heme ligands from six-coordinate, low-spin Fe(II)CooA in forming five-coordina...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991378g

    authors： Reynolds MF,Parks RB,Burstyn JN,Shelver D,Thorsteinsson MV,Kerby RL,Roberts GP,Vogel KM,Spiro TG

    更新日期：2000-01-18 00:00:00

  • Dual-wavelength ratiometric fluorescence measurements of membrane potential.

    abstract：:This work shows that the voltage across membranes in two very different preparations, lipid vesicles in suspension and individual HeLa cells under a microscope, is linearly related to the ratio of fluorescence excited from the two wings of the absorption spectrum of a voltage-sensitive dye. The dye di-4-ANEPPS [1-(3-s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00437a003

    authors： Montana V,Farkas DL,Loew LM

    更新日期：1989-05-30 00:00:00

  • Identification of a region at the N-terminus of phospholipase C-beta 3 that interacts with G protein beta gamma subunits.

    abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992021f

    authors： Barr AJ,Ali H,Haribabu B,Snyderman R,Smrcka AV

    更新日期：2000-02-22 00:00:00

  • Structural transitions of deoxyribonucleic acid in aqueous electrolyte solutions. II. The role of hydration.

    abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00679a018

    authors： Wolf B,Hanlon S

    更新日期：1975-04-22 00:00:00

  • Modification of the conformational equilibria in the sodium and potassium dependent adenosinetriphosphatase with glutaraldehyde.

    abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00288a016

    authors： Chipman DM,Lev A

    更新日期：1983-09-13 00:00:00

  • Water structural changes involved in the activation process of photoactive yellow protein.

    abstract：:Fourier transform infrared (FTIR) spectroscopy was applied to the blue-light photoreceptor photoactive yellow protein (PYP) to investigate water structural changes possibly involved in the photocycle of PYP. Photointermediates were stabilized at low temperature, and difference IR spectra were obtained between intermed...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000357f

    authors： Kandori H,Iwata T,Hendriks J,Maeda A,Hellingwerf KJ

    更新日期：2000-07-11 00:00:00

  • Redox properties of electron-transfer flavoprotein ubiquinone oxidoreductase as determined by EPR-spectroelectrochemistry.

    abstract：:We have determined the formal potential values for each electron transfer to electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO), in order to further characterize the thermodynamics of electron transport from various acyl-CoA thioesters to the mitochondrial ubiquinone pool. ETF-QO contains one [4Fe-4S]2+...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00162a012

    authors： Paulsen KE,Orville AM,Frerman FE,Lipscomb JD,Stankovich MT

    更新日期：1992-12-01 00:00:00

  • Solution structure of the DNA methyl phosphotriester repair domain of Escherichia coli Ada.

    abstract：:The Escherichia coli Ada protein repairs methyl phosphotriesters in DNA by direct, irreversible methyl transfer to one of its own cysteine residues. The methyl-transfer process appears to be autocatalyzed by coordination of the acceptor residue, Cys-69, to a tightly bound zinc ion. Upon methyl transfer, Ada acquires t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00214a003

    authors： Myers LC,Verdine GL,Wagner G

    更新日期：1993-12-28 00:00:00

  • Photooxidation pathway of chlorophyll Z in photosystem II as Studied by Fourier transform infrared spectroscopy.

    abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052346y

    authors： Kitajima Y,Noguchi T

    更新日期：2006-02-14 00:00:00

  • Affinity labeling of lysine-149 in the anion-binding exosite of human alpha-thrombin with an N alpha-(dinitrofluorobenzyl)hirudin C-terminal peptide.

    abstract：:In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00479a006

    authors： Bourdon P,Fenton JW 2nd,Maraganore JM

    更新日期：1990-07-10 00:00:00

  • Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails.

    abstract：:Brd4 belongs to the BET family. It is a multifunctional protein involved in transcription, replication, the signal transduction pathway, and cell cycle progression. All of these functions are linked to its association with acetylated chromatin. With its tandem bromodomains, Brd4 avidly binds to diacetylated H4-AcK5/K1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8001659

    authors： Liu Y,Wang X,Zhang J,Huang H,Ding B,Wu J,Shi Y

    更新日期：2008-06-17 00:00:00

  • Metal ion and substrate binding to bovine galactosyltransferase.

    abstract：:Bovine milk galactosyltransferase was examined by ESR and NMR proton relaxation measurements to determine the stoichiometry and nature of manganese and UDP-Gal substrate binding. The ESR and NMR data clearly showed the binding of two (Mn(II) per mol of enzyme in the ternary complex (enzyme-manganese-UDP-Gal). The affi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00546a017

    authors： Andree PJ,Berliner LJ

    更新日期：1980-03-04 00:00:00

  • Nucleosome Histone Tail Conformation and Dynamics: Impacts of Lysine Acetylation and a Nearby Minor Groove Benzo[a]pyrene-Derived Lesion.

    abstract：:Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b01208

    authors： Fu I,Cai Y,Geacintov NE,Zhang Y,Broyde S

    更新日期：2017-04-11 00:00:00

  • Identification of critical lysyl residues in the pyrophosphate-dependent phosphofructo-1-kinase of Propionibacterium freudenreichii.

    abstract：:Pyrophosphate-dependent 6-phosphofructo-1-kinase (PPi-PFK) from Propionibacterium freudenreichii was inactivated by low concentrations of the lysine-specific reagent pyridoxal phosphate (PLP) after sodium borohydride reduction. The substrates fructose 6-phosphate and fructose 1,6-bisphosphate protected against inactiv...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00135a011

    authors： Green PC,Latshaw SP,Ladror US,Kemp RG

    更新日期：1992-05-26 00:00:00

  • Structure, dynamics, and antimicrobial and immune modulatory activities of human LL-23 and its single-residue variants mutated on the basis of homologous primate cathelicidins.

    abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2016266

    authors： Wang G,Elliott M,Cogen AL,Ezell EL,Gallo RL,Hancock RE

    更新日期：2012-01-17 00:00:00