Structural basis and binding properties of the second bromodomain of Brd4 with acetylated histone tails.

abstract：:Proteolytic processing of the transmembrane domain of the amyloid precursor protein (APP) is a key component of Alzheimer's disease pathogenesis. Using C-terminally tagged APP derivatives, we have identified by amino-terminal sequencing a novel cleavage site of APP, at Leu-49, distal to the gamma-secretase site. This ...

journal_title：Biochemistry

authors： Weidemann A,Eggert S,Reinhard FB,Vogel M,Paliga K,Baier G,Masters CL,Beyreuther K,Evin G

更新日期：2002-02-26 00:00:00

abstract：:Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...

journal_title：Biochemistry

authors： Seto R,Takaichi S,Kurihara T,Kishi R,Honda M,Takenaka S,Tsukatani Y,Madigan MT,Wang-Otomo ZY,Kimura Y

更新日期：2020-06-30 00:00:00

abstract：:The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...

journal_title：Biochemistry

authors： Horváth LI,Brophy PJ,Marsh D

更新日期：1988-01-12 00:00:00

abstract：:Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...

journal_title：Biochemistry

authors： Sepuru KM,Rajarathnam K

更新日期：2018-10-16 00:00:00

abstract：:Triple-helical DNA shows increasing potential for applications in the control of gene expression (including therapeutics) and the development of sequence-specific DNA-cleaving agents. The major limitation in this technology has been the requirement of homopurine sequences for triplex formation. We describe a simple ap...

journal_title：Biochemistry

authors： Jayasena SD,Johnston BH

更新日期：1992-01-21 00:00:00

abstract：:The intense charge transfer complex between the enolate of 3-thia-octanoyl-CoA and the oxidized flavin of the medium-chain acyl-CoA dehydrogenase is discharged by the ferricenium ion with irreversible inactivation of the enzyme. Charge transfer complex formation is a necessary, but insufficient, condition for oxidativ...

journal_title：Biochemistry

authors： Schaller RA,Thorpe C

更新日期：1995-12-19 00:00:00

abstract：:The structures of hemes a and a3 of maize and wheat germ cytochrome c oxidase were investigated by resonance Raman spectroscopy. Comparison between the plant and mammalian cytochrome oxidases revealed that (i) the vinyl groups associated with hemes a and a3 vibrate at higher frequencies in the plant enzyme than in the...

journal_title：Biochemistry

authors： de Paula JC,Peiffer WE,Ingle RT,Centeno JA,Ferguson-Miller S,Babcock GT

更新日期：1990-09-18 00:00:00

abstract：:The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...

journal_title：Biochemistry

authors： Lefferts JA,Wang C,Sridharan D,Baralt M,Lambert MW

更新日期：2009-01-20 00:00:00

abstract：:We previously reported that the microtubule (MT)-binding domain of microtubule-associated protein 4 (MAP4) contains three 18-amino acid imperfect repeats that are homologous to the repeats found in the MT-binding domains of the neuronal MAPs, MAP2 and tau [Chapin, S. J., & Bulinski, J. C. (1991) J. Cell Sci. 98, 27-36...

journal_title：Biochemistry

authors： Chapin SJ,Lue CM,Yu MT,Bulinski JC

更新日期：1995-02-21 00:00:00

abstract：:Although the membrane-associated surfactant protein B (SP-B) is an essential component of lung surfactant, which is itself essential for life, the molecular basis for its activity is not understood. SP-B's biophysical functions can be partially mimicked by subfragments of the protein, including the C-terminus. We have...

journal_title：Biochemistry

authors： Booth V,Waring AJ,Walther FJ,Keough KM

更新日期：2004-12-07 00:00:00

abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

journal_title：Biochemistry

authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

更新日期：2009-10-27 00:00:00

abstract：:Copper-zinc superoxide dismutase (CuZnSOD) acquires its catalytic copper ion through interaction with another polypeptide termed the copper chaperone for SOD. Here, we combine X-ray crystallographic and analytical ultracentrifugation methods to characterize rigorously both truncated and full-length forms of apo-LYS7, ...

journal_title：Biochemistry

authors： Hall LT,Sanchez RJ,Holloway SP,Zhu H,Stine JE,Lyons TJ,Demeler B,Schirf V,Hansen JC,Nersissian AM,Valentine JS,Hart PJ

更新日期：2000-04-04 00:00:00

abstract：:The human double-stranded RNA- (dsRNA) activated protein kinase (PKR) has a dsRNA-binding domain (dsRBD) that contains two tandem copies of the dsRNA-binding motif (dsRBM). The minimal-length polypeptide required to bind dsRNA contains both dsRBMs, as determined by mobility-shift and filter-binding assays. Mobility-sh...

journal_title：Biochemistry

authors： Bevilacqua PC,Cech TR

更新日期：1996-08-06 00:00:00

abstract：:Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...

journal_title：Biochemistry

authors： Swart GW,Parmeggiani A

更新日期：1989-01-10 00:00:00

abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...

journal_title：Biochemistry

authors： Singh M,Castillo D,Patel CV,Patel RC

更新日期：2011-05-31 00:00:00

abstract：:Proelastase has been purified to homogeneity from rat pancreatic tissue by a combination of CM-Sephadex and immobilized protease inhibitor affinity resins. Trypsin activation yields an elastolytic enzyme that possesses a specificity toward small hydrophobic residues in synthetic amide substrates, similar to those of p...

journal_title：Biochemistry

authors： Largman C

更新日期：1983-08-02 00:00:00

abstract：:The C-terminus of interferon-gamma (IFNgamma) contains a nuclear localization sequence (NLS) required for the activation and nuclear translocation of the transcription factor STAT1alpha and induction of IFNgamma-activated genes. On the basis of this and other studies, we developed a peptide mimetic of IFNgamma that po...

journal_title：Biochemistry

authors： Subramaniam PS,Flowers LO,Haider SM,Johnson HM

更新日期：2004-05-11 00:00:00

abstract：:Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or bind...

journal_title：Biochemistry

authors： Di Giambattista M,Engelborghs Y,Nyssen E,Clays K,Cocito C

更新日期：1991-07-23 00:00:00

abstract：:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated...

journal_title：Biochemistry

authors： Viard M,Ablan SD,Zhou M,Veenstra TD,Freed EO,Raviv Y,Blumenthal R

更新日期：2008-02-19 00:00:00

abstract：:Inhibitors of poly(ADP-ribose) polymerase (PARP, EC 2.4.2.30) are of clinical interest because they have potential for improving radiation therapy and chemotherapy of cancer. The refined binding structures of four such inhibitors are reported together with the refined structure of the unligated catalytic fragment of t...

journal_title：Biochemistry

authors： Ruf A,de Murcia G,Schulz GE

更新日期：1998-03-17 00:00:00

abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...

journal_title：Biochemistry

authors： Balsera M,Arellano JB,Gutiérrez JR,Heredia P,Revuelta JL,De Las Rivas J

更新日期：2003-02-04 00:00:00

abstract：:The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation...

journal_title：Biochemistry

authors： Deslauriers R,Komoroski RA,Levy GC,Seely JH,Smith IC

更新日期：1976-10-19 00:00:00

abstract：:One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...

journal_title：Biochemistry

authors： Spezio M,Wilson DB,Karplus PA

更新日期：1993-09-28 00:00:00

abstract：:Nitrophorins 1-4 (NP1-4) are ferriheme proteins from the blood-sucking insect Rhodnius prolixus that transport nitric oxide (NO) to the victim, sequester histamine, and inhibit blood coagulation. Here, we report kinetic and thermodynamic analyses for ligand binding by all four proteins and their reduction potentials. ...

journal_title：Biochemistry

authors： Andersen JF,Ding XD,Balfour C,Shokhireva TK,Champagne DE,Walker FA,Montfort WR

更新日期：2000-08-22 00:00:00

abstract：:We report two small molecule azobenzenesulfonamide probes, CAP1 and CAP2, capable of photomodulating the activity of carbonic anhydrase (CA) on demand. In the trans form, CAP azobenzene probes adopt a linear shape, making them suitable for occupying the CA active site and interacting with Zn2+, thereby inhibiting enzy...

journal_title：Biochemistry

authors： Aggarwal K,Banik M,Medellin B,Que EL

更新日期：2019-01-08 00:00:00

abstract：:The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediat...

journal_title：Biochemistry

authors： Simmons CR,Krishnamoorthy K,Granett SL,Schuller DJ,Dominy JE Jr,Begley TP,Stipanuk MH,Karplus PA

更新日期：2008-11-04 00:00:00

abstract：:The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...

journal_title：Biochemistry

authors： Kanaori K,Nosaka AY

更新日期：1996-10-01 00:00:00

abstract：:A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...

journal_title：Biochemistry

authors： Cappai R,Stewart L,Jobling MF,Thyer JM,White AR,Beyreuther K,Collins SJ,Masters CL,Barrow CJ

更新日期：1999-03-16 00:00:00

abstract：:The SCF ubiquitin ligase associates with substrates through its F-box protein adaptor. Substrates are typically recognized through a defined phosphodegron. Here, we characterize the interaction of the F-box protein Saf1 with Prb1, one of its vacuolar protease substrates. We show that Saf1 binds the mature protein but ...

journal_title：Biochemistry

authors： Mark KG,Meza-Gutierrez F,Johnson JR,Newton BW,Krogan NJ,Toczyski DP

更新日期：2015-07-28 00:00:00

abstract：:Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...

journal_title：Biochemistry

authors： Chai W,Leteux C,Westling C,Lindahl U,Feizi T

更新日期：2004-07-06 00:00:00