Abstract：
:A method to identify poly(L-proline)-type (PII) conformation in crystal structures of globular proteins is presented. Short segments of PII structure were identified in globular protein structures, and these form a significant fraction of the residues which are not assigned to alpha-helix, beta-sheet, and beta-turns. The fractions of alpha-helix, beta-sheet, beta-turns, PII, and unordered, identified in conjunction with the Kabsch and Sander method [(1983) Biopolymers 22, 2577], were incorporated in the analysis of circular dichroism (CD) spectra of proteins. The separation of PII fraction from the fraction of residues not assigned to alpha-helix or beta-sheet or -turns resulted in a distinctive PII CD spectrum and an unusual CD spectrum corresponding to the residual unassigned structures. The quality of prediction of PII fraction from CD spectra of proteins was comparable to that of beta-sheet and -turns.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sreerama N,Woody RWdoi
10.1021/bi00199a028subject
Has Abstractpub_date
1994-08-23 00:00:00pages
10022-5issue
33eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:When alpha,beta-unsaturated substrates bind to the active site of enoyl-CoA hydratase, large spectral changes can be observed [D'Ordine, R. L., et al. (1994) Biochemistry 33, 12635-12643]. The differences in the isotropic magnetic shieldings of the free and active site-bound forms of the carbonyl, alpha-, and beta-car...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015845h
更新日期：2002-02-26 00:00:00
abstract：:Utilizing structure-based design, we have previously demonstrated that it is possible to obtain selective inhibitors of protein-tyrosine phosphatase 1B (PTP1B). A basic nitrogen was introduced into a general PTP inhibitor to form a salt bridge to Asp48 in PTP1B and simultaneously cause repulsion in PTPs containing an ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011389l
更新日期：2001-12-11 00:00:00
abstract：:The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi027103+
更新日期：2003-03-11 00:00:00
abstract：:Voltage-dependent anion channel 1 (VDAC1) situated in the outer mitochondrial membrane regulates the transfer of various metabolites and is a key player in mitochondria-mediated apoptosis. Although many small chemicals that modulate the functions of VDAC1 have been reported to date, most, if not all, of them cannot be...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01209
更新日期：2019-02-26 00:00:00
abstract：:Solution X-ray scattering patterns of enzyme I of the phosphotransferase system from Staphylococcus carnosus indicate an increase in radius of gyration and molecular mass in the presence of Mg2+ or both Mg2+ and phosphoenolpyruvate, indicating a partial dimerization of enzyme I. Mg2+ ions are essential for both the di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00048a014
更新日期：1995-12-05 00:00:00
abstract：:The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00641a013
更新日期：1977-11-01 00:00:00
abstract：:The solution conformation of the ribonuclease barnase has been determined by using 1H nuclear magnetic resonance (NMR) spectroscopy. The 20 structures were calculated by using 853 interproton distance restraints obtained from analyses of two-dimensional nuclear Overhauser spectra, 72 phi and 53 chi 1 torsion angle res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00099a030
更新日期：1991-09-03 00:00:00
abstract：:K(+) channels are revered for their universal action of suppressing electrical activity in nerve and muscle, as well as regulating salt and water transport in epithelial tissues involved in metabolism and digestion. These multisubunit membrane-embedded proteins carry out their physiological chore, selectively allowing...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi8018515
更新日期：2009-01-27 00:00:00
abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981328w
更新日期：1998-10-06 00:00:00
abstract：:Cytochromes c require covalent attachment of heme via two thioether bonds at conserved CXXCH motifs, a process accomplished in prokaryotes by eight integral membrane proteins (CcmABCDEFGH), termed System I. Heme is trafficked from inside the cell to outside (via CcmABCD) and chaperoned (holoCcmE) to the cytochrome c s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00427
更新日期：2016-06-07 00:00:00
abstract：:The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00328a013
更新日期：1985-03-26 00:00:00
abstract：:Chloride intracellular channel protein 1 (CLIC1) functions as an anion channel in plasma and nuclear membranes when its soluble monomeric form converts to an integral-membrane form. The transmembrane region of CLIC1 is located in its thioredoxin-like domain 1, but the mechanism whereby the protein converts to its memb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9010607
更新日期：2009-09-08 00:00:00
abstract：:Moesin binds to a large range of proteins through its N terminal FERM (band 4.1, ezrin, radixin, moesin) domain. In full-length moesin isolated from cells, this binding is masked by binding to the C-terminal domain of moesin (C-ERMAD). Activation takes place by phosphorylation of Thr 558 in the C-ERMAD, which releases...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010419h
更新日期：2001-06-19 00:00:00
abstract：:Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00672
更新日期：2020-10-27 00:00:00
abstract：:Differential expression of subunit genes from the nicotinic acetylcholine receptor (AChR) superfamily yields distinct receptor subtypes. As each AChR subtype has a specific subunit composition and many subunit combinations appear not to be expressed, each subunit must contain some information leading to proper assembl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00254a031
更新日期：1994-12-20 00:00:00
abstract：:Arginine vasopressin (AVP) is a nonapeptide long known as an endocrine and paracrine regulator of important systemic functions, namely, vasoconstriction, gluconeogenesis, corticosteroidogenesis, and excretion of water and urea. Here we report, for the first time, that AVP specifically inhibits expression of the cyclin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026807g
更新日期：2003-02-25 00:00:00
abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0355443
更新日期：2004-03-02 00:00:00
abstract：:Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049196w
更新日期：2004-08-10 00:00:00
abstract：:The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. The structure establishes NicA2 as a member of the monoamine oxidase family. Residues 1-50 are disordered and may play a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00963
更新日期：2016-12-06 00:00:00
abstract：:Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00003a040
更新日期：1995-01-24 00:00:00
abstract：:A generalized set of fluorescence polarization intensities and electron paramagnetic resonance (EPR) spectra from multiple fluorescent and EPR probes of a specific site on an oriented and immobilized protein element in a biological assembly, combined with anisotropic rotational relaxation studies of the purified and l...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00184a004
更新日期：1994-05-10 00:00:00
abstract：:During the past decade, there has been much debate about the enormous catalytic rate enhancement observed in enzymatic reactions involving carbanion intermediates. Our recent theoretical study has demonstrated the importance of the short strong hydrogen bond (SSHB) in the enzymatic reactions. Nevertheless, other recen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0255118
更新日期：2002-04-23 00:00:00
abstract：:Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00243a033
更新日期：1991-07-23 00:00:00
abstract：:UreE is a homodimeric metallo-chaperone that assists the insertion of Ni(2+) ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0601003
更新日期：2006-05-23 00:00:00
abstract：:Sedimentation and gel retardation studies show a stronger interaction of HMG 1 and 2 with negatively supercoiled DNA than with linear, nicked-circular, or positively supercoiled ds-DNA. An apparent unwinding angle of 58 degrees was obtained for HMG 1 and 2 when assayed by protection of negatively supercoiled DNA from ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a005
更新日期：1993-04-06 00:00:00
abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00832
更新日期：2020-12-29 00:00:00
abstract：:α-Synuclein (α-Syn) oligomerization and amyloid formation are associated with Parkinson's disease (PD) pathogenesis. Studying familial α-Syn mutants associated with early onset PD has therapeutic importance. Here we report the aggregation kinetics and other biophysical properties of a newly discovered PD associated Fi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5010365
更新日期：2014-10-21 00:00:00
abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00515a023
更新日期：1981-06-09 00:00:00
abstract：:Circular dichroism spectra of the partially folded trapped intermediates were measured in order to aid in the elucidation of the conformational forces which determine a nonrandom, nonsequential pathway of disulfide bond formation upon refolding of bovine pancreatic trypsin inhibitor. Whatever conformation was responsi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00279a020
更新日期：1983-05-10 00:00:00
abstract：:Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, named protein B1 (170 000) and protein B2 (87 000). We purified and characterized five monoclonal antibodies against B1 and three against B2 from hybridomas obtained by fusion of spleen cells from immunized mice and the myeloma cell ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00352a018
更新日期：1986-02-25 00:00:00
