Chemical tools for K(+) channel biology.

abstract：:The compounds 3-hydroxy-4-nitrobenzaldehyde and 3-hydroxy-4-nitrobenzyl alcohol are introduced as new chromophoric substrates for probing the catalytic mechanism of horse liver alcohol dehydrogenase (LADH). Ionization of the phenolic hydroxyl group shifts the spectrum of the aldehyde from 360 to 433 nm (pKa = 6.0), wh...

journal_title：Biochemistry

authors： MacGibbon AK,Koerber SC,Pease K,Dunn MF

更新日期：1987-06-02 00:00:00

abstract：:The borrelidin polyketide synthase (PKS) begins with a carboxylated substrate and, unlike typical decarboxylative loading PKSs, retains the carboxy group in the final product. The specificity and tolerance of incorporation of carboxyacyl substrate into type I PKSs have not been explored. Here, we show that the first e...

journal_title：Biochemistry

authors： Hagen A,Poust S,de Rond T,Yuzawa S,Katz L,Adams PD,Petzold CJ,Keasling JD

更新日期：2014-09-30 00:00:00

abstract：:Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...

journal_title：Biochemistry

authors： Li M,Satinover DL,Brautigan DL

更新日期：2007-03-06 00:00:00

abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...

journal_title：Biochemistry

authors： Kaur AP,Wilks A

更新日期：2007-03-20 00:00:00

abstract：:Despite recognition that the plasma membrane (PM) is comprised of lipid raft domains that are key organizing sites of multiple signaling pathways and other cell functions, limited information is available regarding the structure and function in sterol dynamics of these microdomains. To begin to resolve these issues, M...

journal_title：Biochemistry

authors： Gallegos AM,Storey SM,Kier AB,Schroeder F,Ball JM

更新日期：2006-10-03 00:00:00

abstract：:Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...

journal_title：Biochemistry

authors： Banci L,Bertini I,De la Rosa MA,Koulougliotis D,Navarro JA,Walter O

更新日期：1998-04-07 00:00:00

abstract：:Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

journal_title：Biochemistry

authors： Negash S,Huang S,Squier TC

更新日期：1999-06-22 00:00:00

abstract：:The trifunctional dinuclear platinum compounds 1,2/c,c [[cis-PtCl(NH(3))(2)]mu-H(2)N(CH(2))(6)NH(2)[cis-PtCl(2)(NH(3))]](+) and 1,2/t,c [[trans-PtCl(NH(3))(2)]mu-H(2)N(CH(2))(6)NH(2)[cis-PtCl(2)(NH(3))]](+) contain a monofunctional platinum coordination sphere linked to a cis-[PtCl(2)(amine)(2)] moiety. The compounds ...

journal_title：Biochemistry

authors： Kloster M,Kostrhunova H,Zaludova R,Malina J,Kasparkova J,Brabec V,Farrell N

更新日期：2004-06-22 00:00:00

abstract：:Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...

journal_title：Biochemistry

authors： Gulbinas V,Karpicz R,Garab G,Valkunas L

更新日期：2006-08-08 00:00:00

abstract：:The catalytic utilization of dimethylallyl, geranyl, farnesyl, and geranylgeranyl diphosphates in the reaction catalyzed by recombinant human farnesyl:protein transferase (hFPTase) has been examined in the presence of three different protein substrates, Ras-CVLS, Ras-CVIM, and Ras-CAIL. hFPTase catalyzed both farnesyl...

journal_title：Biochemistry

authors： Pompliano DL,Schaber MD,Mosser SD,Omer CA,Shafer JA,Gibbs JB

更新日期：1993-08-17 00:00:00

abstract：:In previous work from our laboratory, 5-10% of the channels formed by [Val1]gramicidin A have conductances that fall outside the narrow range that conventionally has defined the standard gramicidin channel [e.g., see Russell et al. (1986) Biophys. J. 49, 673]. Reports from other laboratories, however, show that up to ...

journal_title：Biochemistry

authors： Sawyer DB,Koeppe RE 2nd,Andersen OS

更新日期：1989-08-08 00:00:00

abstract：:The navel orangeworm, Amyelois transitella (Walker), is an agricultural insect pest that can be controlled by disrupting male-female communication with sex pheromones, a technique known as mating disruption. Insect pheromone-binding proteins (PBPs) provide fast transport of hydrophobic pheromones through the aqueous s...

journal_title：Biochemistry

authors： Xu X,Xu W,Rayo J,Ishida Y,Leal WS,Ames JB

更新日期：2010-02-23 00:00:00

abstract：:Currently, it is thought that inhalational anesthetics cause anesthesia by binding to ligand-gated ion channels. This is being investigated using four-alpha-helix bundles, small water-soluble analogues of the transmembrane domains of the "natural" receptor proteins. The study presented here specifically investigates h...

journal_title：Biochemistry

authors： Manderson GA,Michalsky SJ,Johansson JS

更新日期：2003-09-30 00:00:00

abstract：:The thylakoid transmembrane DeltapH is the sole energy source driving translocation of precursor proteins by the DeltapH/Tat machinery. Consequently, proton translocation must be coupled to precursor translocation. For the precursor of the 17 kDa protein of the oxygen-evolving complex (pOE17), the protein translocatio...

journal_title：Biochemistry

authors： Musser SM,Theg SM

更新日期：2000-07-18 00:00:00

abstract：:Succinyl-CoA:3-oxoacid coenzyme A transferase (EC 2.8.3.5), which catalyzes the reversible conversion of succinyl-CoA and acetoacetate into acetoacetyl-CoA and succinate through a covalent enzyme thiol ester intermediate, E-CoA, utilizes binding energy from noncovalent interactions with CoA to bring about an increase ...

journal_title：Biochemistry

authors： Whitty A,Fierke CA,Jencks WP

更新日期：1995-09-19 00:00:00

abstract：:Tumor suppressor Nore1, its acronym coming from novel Ras effector, is one of the 10 members of the Rassf (Ras association domain family) protein family that have been identified. It is expressed as two mRNA splice variants, Nore1A and a shorter isoform, Nore1B. It forms homo- and heterocomplexes through its C-termina...

journal_title：Biochemistry

authors： Makbul C,Constantinescu Aruxandei D,Hofmann E,Schwarz D,Wolf E,Herrmann C

更新日期：2013-02-12 00:00:00

abstract：:The WSXWS motif is a highly conserved structural feature of the type I cytokine receptor family. It has previously been demonstrated that mutations in the (232)WSAWS(236) motif in the erythropoietin receptor (EPOR) can result in strongly inhibited surface expression, due to defective intracellular transport [Hilton, D...

journal_title：Biochemistry

authors： Furmanek A,Hess D,Rogniaux H,Hofsteenge J

更新日期：2003-07-22 00:00:00

abstract：:The chicken and goose egg white lysozymes (ChEWL and GoEWL) are homologues, but differ in substrate specificity. ChEWL catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while GoEWL is specific for bacterial peptidoglycans. The active-site aspartate 52 residue o...

journal_title：Biochemistry

authors： Matsumura I,Kirsch JF

更新日期：1996-02-13 00:00:00

abstract：:O6-Alkylguanine-DNA alkyltransferase (AGT) is an important cellular defense against the mutagenic effects of DNA alkylating agents. In humans this defense can contribute to the ability of some tumors to resist the effects of chemotherapeutic agents that act through DNA alkylation. We report here studies that character...

journal_title：Biochemistry

authors： Fried MG,Kanugula S,Bromberg JL,Pegg AE

更新日期：1996-12-03 00:00:00

abstract：:Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...

journal_title：Biochemistry

authors： Showalter AK,Tsai MD

更新日期：2002-08-27 00:00:00

abstract：:SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...

journal_title：Biochemistry

authors： Williams C,Galyov EE,Bagby S

更新日期：2004-09-28 00:00:00

abstract：:We have studied the kinetics of transfer of glucocerebroside between phospholipid bilayers by using pyrene and 3H-labeled glucocerebroside incorporated into dimyristoylphosphatidylcholine (DMPC) and dipalmitoylphosphatidylcholine (DPPC) bilayers. Pyrene-labeled glucocerebroside (PyrCer) molecules are able to form an e...

journal_title：Biochemistry

authors： Correa-Freire MC,Barenholz Y,Thompson TE

更新日期：1982-03-16 00:00:00

abstract：:Proteolytic fragments of bovine testis calmodulin were obtained by limited proteolysis with trypsin or thrombin. Cadmium-113 NMR studies showed that the tryptic fragment encompassing Ca2+ binding domains III and IV (TR2C) gave rise to a spectrum identical with that of the native protein. Two thrombic fragments contain...

journal_title：Biochemistry

authors： Andersson A,Forsén S,Thulin E,Vogel HJ

更新日期：1983-05-10 00:00:00

abstract：:The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...

journal_title：Biochemistry

authors： Khouri HE,Vernet T,Ménard R,Parlati F,Laflamme P,Tessier DC,Gour-Salin B,Thomas DY,Storer AC

更新日期：1991-09-17 00:00:00

abstract：:To shed more light on the molecular requirements for recognition of thyroid response elements (TREs) by thyroid receptors (TRs), we compared the specific aspects of DNA TRE recognition by different TR constructs. Using fluorescence anisotropy, we performed a detailed and hierarchical study of TR-TRE binding. This was ...

journal_title：Biochemistry

authors： Figueira AC,Lima LM,Lima LH,Ranzani AT,Mule Gdos S,Polikarpov I

更新日期：2010-02-09 00:00:00

abstract：:The turnover of the collagen triple-helical structure (collagenolysis) is a tightly regulated process in normal physiology and has been ascribed to a small number of proteases. Several members of the matrix metalloproteinase (MMPs) family possess collagenolytic activity, and the mechanisms by which these enzymes proce...

journal_title：Biochemistry

authors： Minond D,Lauer-Fields JL,Cudic M,Overall CM,Pei D,Brew K,Moss ML,Fields GB

更新日期：2007-03-27 00:00:00

abstract：:The following peptide was synthesized by classical methods in solution: Ac-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg-Gly-Pro-Arg-Val-NHCH3 (F-8). The Michaelis-Menten parameters for the hydrolysis of the Arg-Gly bond in F-8 by thrombin were determined to be Kcat = 31 X 10(-11) M [(NIH unit/L) s]-1 and KM = 310 X 10(-6) ...

journal_title：Biochemistry

authors： Marsh HC Jr,Meinwald YC,Thannhauser TW,Scheraga HA

更新日期：1983-08-30 00:00:00

abstract：:Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...

journal_title：Biochemistry

authors： Mu H,Kropachev K,Chen Y,Zhang H,Cai Y,Geacintov NE,Broyde S

更新日期：2013-08-20 00:00:00

abstract：:Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...

journal_title：Biochemistry

authors： Wadkins RM,Graves DE

更新日期：1991-04-30 00:00:00

abstract：:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...

journal_title：Biochemistry

authors： Chi LM,Lam SL

更新日期：2009-12-08 00:00:00