Cadmium-113 nuclear magnetic resonance studies of proteolytic fragments of calmodulin: assignment of strong and weak cation binding sites.

abstract：:Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been previously reported that the soluble extracellular domains of the EPO receptor (sEPOR) are not dimerized in the presence of EPO and are able to form only 1:1 complex...

journal_title：Biochemistry

authors： Philo JS,Aoki KH,Arakawa T,Narhi LO,Wen J

更新日期：1996-02-06 00:00:00

abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...

journal_title：Biochemistry

authors： McCutchen SL,Colon W,Kelly JW

更新日期：1993-11-16 00:00:00

abstract：:Subunit II of the aa3 type cytochrome c oxidase contains a binuclear copper center (CuA) which functions as the entry point for electrons donated by cytochrome c. We have introduced site-specific mutations in residues liganding the CuA center in the oxidase of the bacterium Paracoccus denitrificans; the purified, full...

journal_title：Biochemistry

authors： Zickermann V,Wittershagen A,Kolbesen BO,Ludwig B

更新日期：1997-03-18 00:00:00

abstract：:The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5...

journal_title：Biochemistry

authors： Sy J

更新日期：1975-03-11 00:00:00

abstract：:Alkalimetric, spectrophotometric, NMR, and resonance Raman titrations are reported for the sulfonamide Neoprontosil in aqueous solution. An assignment of the magnetic resonance peaks for each of the Neoprontosil protons has been made. Neoprontosil is shown to have two "coupled" iity of the microscopic pKs for these tw...

journal_title：Biochemistry

authors： Carey PR,King RW

更新日期：1979-06-26 00:00:00

abstract：:8-OxoGua (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to correspondi...

journal_title：Biochemistry

authors： Ito R,Hayakawa H,Sekiguchi M,Ishibashi T

更新日期：2005-05-03 00:00:00

abstract：:Surface plasmon resonance (SPR) detection technology was employed to investigate the kinetic mechanism of deoxyadenosine kinase from Mycoplasma mycoides ssp. mycoides SC. In our experimental approach, the enzyme was attached to the sensor surface, the reactants were injected in the mobile phase, and the product-enzyme...

journal_title：Biochemistry

authors： Pol E,Wang L

更新日期：2006-01-17 00:00:00

abstract：:The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction. In this study, we used electrochemically induced FTIR difference spectroscopy to identify residues involved in redox-couple...

journal_title：Biochemistry

authors： Hellwig P,Gomes CM,Teixeira M

更新日期：2003-05-27 00:00:00

abstract：:A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein...

journal_title：Biochemistry

authors： Van Heeke G,Deforce L,Schnizer RA,Shaw R,Couton JM,Shaw G,Song PS,Schuster SM

更新日期：1993-09-28 00:00:00

abstract：:During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A reso...

journal_title：Biochemistry

authors： Mueller U,Maier G,Mochi Onori A,Cellai L,Heumann H,Heinemann U

更新日期：1998-09-01 00:00:00

abstract：:To investigate the possible role of viral glycoprotein mobility in membrane fusion, fluorescence photobleaching recovery was employed to study the effects of exposure to mildly acidic pH (required to convert many viral fusion proteins to the fusion-active form) on the lateral mobility of influenza hemagglutinin (HA) p...

journal_title：Biochemistry

authors： Gutman O,Danieli T,White JM,Henis YI

更新日期：1993-01-12 00:00:00

abstract：:Triclinic crystals of hen egg-white lysozyme cross-linked with glutaraldehyde have been treated with various denaturants and found to be susceptible to x-ray structure analysis even after major conformational changes in the protein. Cross-linked crystals were isomorphous with the native form, and electron density diff...

journal_title：Biochemistry

authors： Yonath A,Sielecki A,Moult J,Podjarny A,Traub W

更新日期：1977-04-05 00:00:00

abstract：:The function of the GM2 activator protein is to act as a substrate-specific cofactor in the hydrolysis of GM2 ganglioside by beta-hexosaminidase A. Mutations in the gene encoding it result in the AB variant form of GM2 gangliosidosis. One such mutation, Cys138 Arg, results in the mutant protein being retained and degr...

journal_title：Biochemistry

authors： Xie B,Rigat B,Smiljanic-Georgijev N,Deng H,Mahuran D

更新日期：1998-01-20 00:00:00

abstract：:The expression of the transforming gene product of Rous sarcoma virus (pp60v-src) in Saccharomyces cerevisiae has recently been reported (Kornbluth et al., 1987; Brugge et al., 1987). To carry out biochemical and structural studies of this enzyme, a facile purification was developed. The purification was accomplished ...

journal_title：Biochemistry

authors： Radziejewski C,Miller WT,Mobashery S,Goldberg AR,Kaiser ET

更新日期：1989-11-14 00:00:00

abstract：:The binding of the codon UUC to the isolated anticodon loop of tRNAPhe (yeast) has been studied as a model of codon recognition by a simple adaptor. Fluorescence titrations demonstrate that UUC binds to the isolated anticodon loop with an equilibrium constant of 1.4 X 10(3) M-1 (at 7.2 degrees C). Equilibrium sediment...

journal_title：Biochemistry

authors： Bujalowski W,Jung M,McLaughlin LW,Porschke D

更新日期：1986-10-21 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:We report a straightforward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity and inhibition of fucosyltransferase VIII, which catal...

journal_title：Biochemistry

authors： Soroko M,Kwan DH

更新日期：2020-06-09 00:00:00

abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...

journal_title：Biochemistry

authors： Askerka M,Vinyard DJ,Brudvig GW,Batista VS

更新日期：2015-09-29 00:00:00

abstract：:Aqueous suspensions of dark-adapted bacteriorhodopsin (bR560) in the purple membrane of Halobacterium halobium are exposed to rapid jumps to high pH. Optical and resonance Raman measurements are carried out by using flow and stationary methods. Above pH congruent to 11.5 bR560 starts to be reversibly converted to a sp...

journal_title：Biochemistry

authors： Druckmann S,Ottolenghi M,Pande A,Pande J,Callender RH

更新日期：1982-09-28 00:00:00

abstract：:MeCP2 has been identified as a chromatin-associated protein that recognizes MAR elements as well as methyl-CpGs. To characterize target sequences of MeCP2 in human cells, we employed two complementary methods. First, by use of a preparative chromatin immunoprecipitation protocol, we created from MCF7 cells a library e...

journal_title：Biochemistry

authors： Koch C,Strätling WH

更新日期：2004-05-04 00:00:00

abstract：:The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...

journal_title：Biochemistry

authors： Falcon CM,Matthews KS

更新日期：2001-12-25 00:00:00

abstract：:The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...

journal_title：Biochemistry

authors： Zimmermann JL,Amano T,Sigalat C

更新日期：1999-11-16 00:00:00

abstract：:In order to gain insight into the structure of human parathyroid hormone (hPTH), four fragments [hPTH(1-34), hPTH(18-48), hPTH(28-48), and hPTH(53-84)], which cover all regions of the intact hormone, have been investigated by CD and NMR spectroscopy in combination with distance geometry, and restrained molecular dynam...

journal_title：Biochemistry

authors： Wray V,Federau T,Gronwald W,Mayer H,Schomburg D,Tegge W,Wingender E

更新日期：1994-02-22 00:00:00

abstract：:Extensive molecular modeling with molecular dynamics simulations and van der Waals energy analyses were used to elucidate the striking finding that a mutagenic benzo[a]pyrene-derived DNA lesion, the base-displaced intercalated 10R-(+)-cis-anti-B[a]P-N(2)-dG (G*), manifests large differences in nucleotide excision repa...

journal_title：Biochemistry

authors： Mu H,Kropachev K,Chen Y,Zhang H,Cai Y,Geacintov NE,Broyde S

更新日期：2013-08-20 00:00:00

abstract：:The fungal neurotoxin alpha-cyclopiazonic acid (CPA), a nanomolar inhibitor of Ca(2+)-ATPase with a unique pentacyclic indole tetramic acid scaffold, is assembled by a three enzyme pathway CpaS, CpaD, and CpaO in Aspergillus sp. We recently characterized the first pathway-specific enzyme CpaS, a hybrid two module poly...

journal_title：Biochemistry

authors： Liu X,Walsh CT

更新日期：2009-11-24 00:00:00

abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...

journal_title：Biochemistry

authors： Fiordalisi JJ,al-Rabiee R,Chiappinelli VA,Grant GA

更新日期：1994-04-05 00:00:00

abstract：:The binding site of the dopamine D2 receptor, like that of other homologous G protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Using the substituted-cysteine accessibility method, we previously mapped the residues in the third, fifth, and seve...

journal_title：Biochemistry

authors： Javitch JA,Ballesteros JA,Weinstein H,Chen J

更新日期：1998-01-27 00:00:00

abstract：:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

journal_title：Biochemistry

authors： Leroy JL,Charretier E,Kochoyan M,Guéron M

更新日期：1988-12-13 00:00:00

abstract：:Nitrogenase reduces N2 to NH3, but the mechanistic details are unclear. Diazene (N2H2), a proposed 2e-/2H+ intermediate on the reduction pathway, is labile under typical enzyme assay conditions, and no firm evidence is available on whether or not it can be reduced by or inhibit nitrogenase. In this paper, we compare t...

journal_title：Biochemistry

authors： McKenna CE,Simeonov AM,Eran H,Bravo-Leerabhandh M

更新日期：1996-04-09 00:00:00

abstract：:We have examined the ability of the Escherichia coli single-stranded DNA binding protein (SSB) tetramer to form its different binding modes on poly(dC), poly(U), and poly(A) over a range of NaCl and NaF concentrations for comparison with previous studies with poly(dT). In reverse titrations with poly(U) and poly(A) at...

journal_title：Biochemistry

authors： Lohman TM,Bujalowski W

更新日期：1994-05-24 00:00:00