Abstract：
:MeCP2 has been identified as a chromatin-associated protein that recognizes MAR elements as well as methyl-CpGs. To characterize target sequences of MeCP2 in human cells, we employed two complementary methods. First, by use of a preparative chromatin immunoprecipitation protocol, we created from MCF7 cells a library enriched with sequences bound to MeCP2. A total of 154 representative clones were sequenced and analyzed. A large fraction of clones was found to be associated with retrotransposons, mostly with Alu repeats. A subgroup of four clones is derived from putative MARs; one clone is associated with a CpG island, and four clones contain alphoid repeats. Classical satellite DNAs II and III are not represented among clones, although they are heavily methylated. Second, using indirect immunofluorescence microscopy, we show that MeCP2 staining of human metaphase chromosomes has a dotted to knobby appearance with a reduced level of staining of centromeric regions of some chromosomes. On the other hand, an anti-5-methylcytosine antibody preferentially stained the juxtacentromeric regions of chromosomes 1, 9, and 16, which habor highly methylated, classical satellite DNAs, and methylated alphoid sequences in centromeric regions of several other chromosomes with reduced intensity. In interphase MCF7 cells, the distribution of MeCP2 exhibits a granular appearance throughout the nucleus. This distribution does not parallel that of methylated cytosine and heterochromatin. The selective binding behavior of MeCP2 revealed by these results (preference for murine major satellite DNA, Alu sequences, MARs, and CpG islands) is explained by its ability to recognize the sequence information (guanine bases) adjacent to CpG (TpG) as demonstrated in previous footprinting experiments.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Koch C,Strätling WHdoi
10.1021/bi0359271subject
Has Abstractpub_date
2004-05-04 00:00:00pages
5011-21issue
17eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Chorismate-utilizing enzymes catalyze diverse reactions, providing critical physiological functions unique to plants, bacteria, fungi, and some parasites. Their absence in animals makes them excellent targets for antimicrobials and herbicides. 4-Amino-4-deoxychorismate synthase (ADCS) catalyzes the first step in folat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052216p
更新日期：2006-04-18 00:00:00
abstract：:Nhp6A is an abundant non-histone chromatin-associated protein in Saccharomyces cerevisiae that contains a minor groove DNA binding motif called the HMG box. In this report, we show that Nhp6Ap binds to cisplatin intrastrand cross-links on duplex DNA with a 40-fold greater affinity than to unmodified DNA with the same ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012077l
更新日期：2002-04-30 00:00:00
abstract：:A new rapid procedure for purifying 10-formyltetrahydrofolate dehydrogenase results in 90 mg of pure enzyme from two rabbit livers. This abundant liver enzyme is known to bind its product tetrahydropteroylpentaglutamate (H4PteGlu5) so tightly that it does not dissociate during size exclusion chromatography. 10-Formylt...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9619684
更新日期：1996-12-10 00:00:00
abstract：:The interaction of Ca2+ and vanadate with fluorescein isothiocyanate (FITC) labeled sarcoplasmic reticulum (SR) Ca2+-ATPase has been studied by following the kinetics of changes in the reporter group fluorescence and equilibrium fluorescence levels. The vanadate species bound to the enzyme is clearly monomeric orthova...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a057
更新日期：1989-01-24 00:00:00
abstract：:Copper binding to the human copper chaperone for superoxide dismutase (hCCS) has been investigated by X-ray absorption spectroscopy. Stoichiometry measurements on the dialyzed, as-isolated protein indicated that up to 3.5 Cu ions bound per hCCS molecule. Reduction with either sodium dithionite or dithiothreitol decrea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000690j
更新日期：2000-06-27 00:00:00
abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401103k
更新日期：2013-12-03 00:00:00
abstract：:The membrane topology model of the Na(+)-citrate transporter CitS of Klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. A split version of CitS was constructed to study domain interactions and proximity relationships of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100336s
更新日期：2010-06-01 00:00:00
abstract：:A new actinoleukin-like antitumor antibiotic, BBM-928A, has been shown to interact with isolated DNA molecules. BBM-928A contains two substituted quinolines linked by a cyclic decapeptide. Quenching effects of the covalently closed superhelical PM2 DNA on the BBM-928A fluorescence revealed a strong interaction with an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00565a012
更新日期：1980-11-25 00:00:00
abstract：:The kinetics of the slow folding and unfolding reactions of barstar, a bacterial ribonuclease inhibitor protein, have been studied at 23(+/-1) degrees C, pH 8, by the use of tryptophan fluorescence, far-UV circular dichroism (CD), near-UV CD, and transient mixing (1)H nuclear magnetic resonance (NMR) spectroscopic mea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990285w
更新日期：1999-07-13 00:00:00
abstract：:The three subunits I, II, and III of bovine procarboxypeptidase A separated by reversible dimethylmaleylation can reassociate to form the reconstituted complexes I + II, I + III, and I + II + III. Since the association II + III is not possible, subunit I appears to play a central role in the formation of the complex. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00630a028
更新日期：1977-05-31 00:00:00
abstract：:Human alpha1 and alpha2 isoforms of Na,K-ATPase have been expressed with porcine 10*Histidine-tagged beta1 subunit in Pichia pastoris. Methanol-induced expression of alpha2 is optimal at 20 degrees C, whereas at 25 degrees C, which is optimal for expression of alpha1, alpha2 is not expressed. Detergent-soluble alpha2b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701812c
更新日期：2007-12-25 00:00:00
abstract：:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101806b
更新日期：2011-04-19 00:00:00
abstract：:The binding of Ca2+ and the resulting change in catalytic specificity that allows phosphorylation of the calcium ATPase of sarcoplasmic reticulum by ATP were examined by measuring the amount of phosphoenzyme formation from [32P]ATP, or 45Ca incorporation into vesicles, after the simultaneous addition of ATP and EGTA a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00423a018
更新日期：1988-11-15 00:00:00
abstract：:The interaction of glutamyl-tRNA synthetase with tRNAGlu2 has been studied. The enzyme was purified to apparent homogeneity, and consists of a single chain with a molecular weight of 59 000. The sedimentation coefficient (sdegrees20,w) was found to be 3.7 S and suggests this enzyme is quite asymmetric. The enzyme bind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00664a032
更新日期：1976-09-21 00:00:00
abstract：:The enzyme 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (EC 4.1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was inactivated by the thiol-m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952373w
更新日期：1996-07-09 00:00:00
abstract：:Phosphofructokinase from Escherichia coli (EcPFK) is a homotetramer with four active sites and four allosteric sites. Understanding the allosteric activation of EcPFK by MgADP has been complicated by the complex web of possible interactions, including active site homotropic interactions, allosteric site homotropic int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048569q
更新日期：2004-11-09 00:00:00
abstract：:Minor groove specific DNA equilibrium binding peptides (lex) based on N-methylpyrrole-carboxamide and/or N-methylimidazolecarboxamide subunits have been modified with an O-methyl sulfonate ester functionality to target DNA methylation in the minor groove at Ade/Thy- and/or Gua/Cyt-rich sequences. HPLC and sequencing g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0353272
更新日期：2003-12-09 00:00:00
abstract：:Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent on the presence of negatively charged lipids. By using a phospholipid with an acyl chain linked photoactivatable group,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9809021
更新日期：1998-09-01 00:00:00
abstract：:The conformational properties of purine-pyrimidine and pyrimidine-purine dinucleoside monophosphates in which the glycosidic torsion is fixed to congruent to 120 degree by the formation of a covalent link between the base and the sugar ring are explored by 1H NMR spectroscopy in order to obtain information about the s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00520a029
更新日期：1981-08-18 00:00:00
abstract：:Membrane-bound mammalian adenylyl cyclase isoforms contain two internally homologous cytoplasmic domains (C1 and C2). When expressed separately, C1 and C2 are catalytically inactive, but conversion of ATP to cAMP is observed if C1 and C2 are combined. By analogy with DNA polymerases, adenylyl cyclases are thought to r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981441m
更新日期：1998-11-17 00:00:00
abstract：:13C NMR spectroscopy has been used to study the amino group environments and metal binding properties of 13C reductively methylated bovine alpha-lactalbumin. Bovine alpha-lactalbumin is a Ca2+ metalloprotein containing 12 lysyl amino groups and a free amino terminus. All 13 amino groups can be 13C-dimethylated without...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00315a026
更新日期：1984-09-25 00:00:00
abstract：:Heparan sulfate (HS) proteoglycans (PGs) interact with a number of extracellular signaling proteins, thereby playing an essential role in the regulation of many physiological processes. These interactions are important for both normal signal transduction and regulation of the tissue distribution of signaling molecules...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6025424
更新日期：2007-04-03 00:00:00
abstract：:Previously [Luz et al. (1994) Biochemistry 33, 7239-7249], we determined that Cl(-)- and -HCO3-dependent pHi homeostasis was perturbed in multidrug resistant (MDR) cells created by transfecting LR73 Chinese hamster ovary fibroblasts with wild-type mu (murine) MDR 1 (Gros et al., 1991). Via single-cell photometry exper...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00202a021
更新日期：1994-09-13 00:00:00
abstract：:Phospholipid transfer protein (PLTP) is expressed by macrophage-derived foam cells in human atherosclerotic lesions, suggesting a regulatory role for PLTP in cellular cholesterol homeostasis. However, the exact role of PLTP in the reverse cholesterol transport pathway is not known. PLTP is present in plasma as two for...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700833h
更新日期：2007-10-23 00:00:00
abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00179a011
更新日期：1994-04-05 00:00:00
abstract：:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971707+
更新日期：1998-04-14 00:00:00
abstract：:Integral membrane proteins, which include many cellular effector proteins and drug targets, can be difficult to produce, purify, and manipulate. Although the isolated ectodomains of many membrane proteins can be expressed as water soluble proteins, biological activity is frequently lost when these proteins are release...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052600m
更新日期：2006-03-21 00:00:00
abstract：:It has been suggested that the down-regulation of hepatic HMG-CoA reductase by dietary cholesterol requires modification of the cholesterol molecule before it can exert its suppressive action. In a recent study [Lund, E., Breuer, O., & Björkhem, I. (1992) J. Biol. Chem. 267, 25092-25097], we showed that side-chain hyd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00167a038
更新日期：1994-01-11 00:00:00
abstract：:The field of chemical site-selective modification of proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use of site-selective protein chemistry to probe the ro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00639
更新日期：2018-10-30 00:00:00
abstract：:The ion binding parameters of the EF-hand Ca2+ binding motif are carefully tuned for different biological applications. The present study examines the contribution of the ninth position of the Ca2+-coordinating EF-loop to the tuning of Ca2+ affinity and selectivity, using the model EF-loop of the Escherichia coli gala...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952430l
更新日期：1996-05-28 00:00:00
