The C2 catalytic domain of adenylyl cyclase contains the second metal ion (Mn2+) binding site.

abstract：:Several 3-halo-3-(1-haloalkyl)-1(3H)-isobenzofuranones, 3-(1-haloalkylidene)-1(3H)-isobenzofuranones, and 3-bromomethyl-1H-2-benzopyran-1-ones containing masked halo ketone functional groups were synthesized and tested as inhibitors of several serine proteases including human leukocyte (HL) elastase and cathepsin G. W...

journal_title：Biochemistry

authors： Hemmi K,Harper JW,Powers JC

更新日期：1985-04-09 00:00:00

abstract：:Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...

journal_title：Biochemistry

authors： Baer BR,Schuman JT,Campbell AP,Cheesman MJ,Nakano M,Moguilevsky N,Kunze KL,Rettie AE

更新日期：2005-10-25 00:00:00

abstract：:We are attempting to understand the processes required to accurately replicate the repetitive DNA sequences whose instability is associated with several human diseases. Here we test the hypothesis that the contribution of exonucleolytic proofreading to frameshift fidelity during replication of repetitive DNA sequences...

journal_title：Biochemistry

authors： Kroutil LC,Register K,Bebenek K,Kunkel TA

更新日期：1996-01-23 00:00:00

abstract：:Tyrosine phenol-lyase, a tetrameric pyridoxal 5'-phosphate dependent enzyme, catalyzes the reversible hydrolytic cleavage of L-tyrosine to phenol and ammonium pyruvate. Here we describe the crystal structure of the Citrobacter freundii holoenzyme at 1.9 A resolution. The structure reveals a network of protein interact...

journal_title：Biochemistry

authors： Milić D,Matković-Calogović D,Demidkina TV,Kulikova VV,Sinitzina NI,Antson AA

更新日期：2006-06-20 00:00:00

abstract：:We have determined the fidelity of in vitro DNA synthesis catalyzed at high temperature by the DNA polymerase from the thermophilic bacterium Thermus aquaticus. Using a DNA substrate that contains a 3'-OH terminal mismatch, we demonstrate that the purified polymerase lacks detectable exonucleolytic proofreading activi...

journal_title：Biochemistry

authors： Tindall KR,Kunkel TA

更新日期：1988-08-09 00:00:00

abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

journal_title：Biochemistry

authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

更新日期：2006-09-05 00:00:00

abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...

journal_title：Biochemistry

authors： Gross A,Hubbell WL

更新日期：2002-01-29 00:00:00

abstract：:The kinetics of folding/unfolding of cross-linked Lys7-dinitrophenylene-Lys41-ribonuclease A were studied and compared to those of unmodified ribonuclease A (RNase A) at various concentrations of guanidine hydrochloride. The folding of the denatured cross-linked protein involved one fast-folding species (22 +/- 4%) an...

journal_title：Biochemistry

authors： Lin SH,Konishi Y,Nall BT,Scheraga HA

更新日期：1985-05-21 00:00:00

abstract：:The kinetics of inhibition of dialkylglycine decarboxylase by five aminophosphonate inhibitors are presented. Two of these [(R)-1-amino-1-methylpropanephosphonate and (S)-1-aminoethanephosphonate] are slow binding inhibitors. The inhibitors follow a mechanism in which a weak complex is rapidly formed, followed by slow...

journal_title：Biochemistry

authors： Liu W,Rogers CJ,Fisher AJ,Toney MD

更新日期：2002-10-15 00:00:00

abstract：:Fructose-1,6-bisphosphate muscle aldolase is an essential glycolytic enzyme that catalyzes reversible carbon-carbon bond formation by cleaving fructose 1,6-bisphosphate to yield dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde phosphate. To elucidate the mechanistic role of conserved amino acid Asp-33, Asn-33 an...

journal_title：Biochemistry

authors： St-Jean M,Blonski C,Sygusch J

更新日期：2009-06-02 00:00:00

abstract：:Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...

journal_title：Biochemistry

authors： Longo A,Guanga GP,Rose RB

更新日期：2007-03-20 00:00:00

abstract：:Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...

journal_title：Biochemistry

authors： Meinke C,Solé VA,Pospisil P,Dau H

更新日期：2000-06-20 00:00:00

abstract：:Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...

journal_title：Biochemistry

authors： Ohgaki R,van IJzendoorn SC,Matsushita M,Hoekstra D,Kanazawa H

更新日期：2011-02-01 00:00:00

abstract：:Spectrophotometric titration of pyridoxamine-pyruvate transaminase (EC 2.6.1.30) with pyridoxal at pH 7.15 gives four equivalent binding sites per tetramer. The pH dependence of the equilibrium constant for the association of 5'-deoxypyridoxal with the active site lysine residue was determined spectrophotometrically. ...

journal_title：Biochemistry

authors： Gilmer PJ,McIntire WS,Kirsch JF

更新日期：1977-11-29 00:00:00

abstract：:The catalytic mechanism of valyl-tRNA synthetase from baker's yeast has been investigated by pre-steady-state and steady-state kinetic measurements and end product dissociation studies. The pre-steady-state kinetics show a lag period during the early time when the reaction is started with free enzyme. The preincubatio...

journal_title：Biochemistry

authors： Kern D,Gangloff J

更新日期：1981-04-14 00:00:00

abstract：:The catalytic mechanism of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) and a derivative of rsAPX in which a cysteine residue (Cys32) located close to the substrate (L-ascorbic acid) binding site has been modified to preclude binding of ascorbate [Mandelman, D., Jamal, J., and Poulos, T. L. (1998) Bioche...

journal_title：Biochemistry

authors： Lad L,Mewies M,Raven EL

更新日期：2002-11-19 00:00:00

abstract：:An aminoglycoside 3-acetyltransferase [AAC(3)], possibly a new isoenzymic species of the 3-N-acetyltransferase group, was purified to apparent homogeneity from a crude extract of Pseudomonas aeruginosa, a gentamicin-resistant clinical isolate. The method of purification was consecutive column chromatography--(i) gel f...

journal_title：Biochemistry

authors： Coombe RG,George AM

更新日期：1982-03-02 00:00:00

abstract：:The presence of a N-(2-deoxy-D-erythro pentofuranosyl)urea (henceforth referred to as deoxyribosylurea) residue, ring fragmentation product of a thymine, in a frameshift situation in the sequence 5'd(AGGACCACG).d(CGTGGurTCCT) has been studied by 1H and 31P nuclear magnetic resonance and molecular dynamics. At equilibr...

journal_title：Biochemistry

authors： Gervais V,Cognet JA,Guy A,Cadet J,Téoule R,Fazakerley GV

更新日期：1998-01-27 00:00:00

abstract：:Sulfonylureas and glinides are commonly used oral insulin secretagogues (ISs) that act on the pancreatic ATP-sensitive potassium (KATP) channel to promote insulin secretion in order to lower the blood glucose level. Physiologically, KATP channels are inhibited by intracellular ATP and activated by Mg-ADP. Therefore, t...

journal_title：Biochemistry

authors： Wu JX,Ding D,Wang M,Chen L

更新日期：2020-01-14 00:00:00

abstract：:The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...

journal_title：Biochemistry

authors： Doukov T,Li H,Soltis M,Poulos TL

更新日期：2009-11-03 00:00:00

abstract：:Targeting damage to specific sites in the genome represents an attractive approach to manipulating gene function in mammalian cells. To test the applicability of triple-helix formation as a means for achieving precisely timed site-specific damage within a mammalian gene, a triplex-forming oligodeoxyribonucleotide (TFO...

journal_title：Biochemistry

authors： Vasquez KM,Wensel TG,Hogan ME,Wilson JH

更新日期：1996-08-20 00:00:00

abstract：:Stopped-flow and laser photolysis methods have been used to investigate the rates of electron-transfer reactions of fully reduced riboflavin and the three oxidation states of Clostridium pasteurianum flavodoxin. Both normal and 7,8-dichloroflavin analogues were studied. Redox reagents included oxygen, ferricyanide, fe...

journal_title：Biochemistry

authors： Jung J,Tollin G

更新日期：1981-09-01 00:00:00

abstract：:Complete proton assignments are reported for the 1H nuclear magnetic resonance (NMR) spectrum of Escherichia coli thioredoxin in the oxidized (with active-site disulfide bridge) and reduced (with two sulfhydryl groups) states. The assignments were obtained by using an integrated assignment strategy in which spin syste...

journal_title：Biochemistry

authors： Dyson HJ,Holmgren A,Wright PE

更新日期：1989-08-22 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...

journal_title：Biochemistry

authors： Fredenburg RA,Rospigliosi C,Meray RK,Kessler JC,Lashuel HA,Eliezer D,Lansbury PT Jr

更新日期：2007-06-19 00:00:00

abstract：:Rett syndrome causing missense mutations in the methyl-CpG-binding domain (MBD) of methyl CpG-binding protein 2 (MeCP2) were investigated both in silico and in vitro to reveal their effect on protein stability. It is demonstrated that the vast majority of frequently occurring mutations in the human population indeed a...

journal_title：Biochemistry

authors： Kucukkal TG,Yang Y,Uvarov O,Cao W,Alexov E

更新日期：2015-10-20 00:00:00

abstract：:Sensory rhodopsin II (SRII) is unique among the archaeal rhodopsins in having an absorption maximum near 500 nm, blue shifted roughly 70 nm from the other pigments. In addition, SRII displays vibronic structure in the lambda(max) absorption band, whereas the other pigments display fully broadened band maxima. The mole...

journal_title：Biochemistry

authors： Ren L,Martin CH,Wise KJ,Gillespie NB,Luecke H,Lanyi JK,Spudich JL,Birge RR

更新日期：2001-11-20 00:00:00

abstract：:Colicin E3 is a 60 kDa, multidomain protein antibiotic that targets its ribonuclease activity to an essential region of the 16S ribosomal RNA of Escherichia coli. To prevent suicide of the producing cell, synthesis of the toxin is accompanied by the production of a 10 kDa immunity protein (Im3) that binds strongly to ...

journal_title：Biochemistry

authors： Walker D,Moore GR,James R,Kleanthous C

更新日期：2003-04-15 00:00:00

abstract：:The structure of a novel plant defensin isolated from the flowers of Petunia hybrida has been determined by (1)H NMR spectroscopy. P. hybrida defensin 1 (PhD1) is a basic, cysteine-rich, antifungal protein of 47 residues and is the first example of a new subclass of plant defensins with five disulfide bonds whose stru...

journal_title：Biochemistry

authors： Janssen BJ,Schirra HJ,Lay FT,Anderson MA,Craik DJ

更新日期：2003-07-15 00:00:00

abstract：:Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...

journal_title：Biochemistry

authors： Vachon G,Laalami S,Grunberg-Manago M,Julien R,Cenatiempo Y

更新日期：1990-10-16 00:00:00