Abstract:
:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the lifetimes of the central A.T base pairs of the dodecamer 5'-d-CGCAAAAAAGCG are 122 and 91 ms at 15 degrees C whereas, in the same conditions, the lifetime of the central A.T pair of the decamer 5'-d-CGCGATCGCG is only 4 ms, a value similar to those measured in several other B-DNA oligoduplexes [Leroy et al. (1988) J. Mol. Biol. 200, 223-238]. This strongly suggests that, in tracts of four A.T pairs or more, a conformation distinct from standard B-DNA is formed cooperatively. All sequences known to generate curved DNA exhibit anomalously long base-pair lifetimes. This is the first local and physical property shown to correlate with DNA curvature. Our observations suggest that the structure responsible for the long lifetimes is involved in the curvature of DNA.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Leroy JL,Charretier E,Kochoyan M,Guéron Mdoi
10.1021/bi00425a004subject
Has Abstractpub_date
1988-12-13 00:00:00pages
8894-8issue
25eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00031a033
更新日期:1995-08-08 00:00:00
abstract::The HAMP domain is a conserved motif widely distributed in prokaryotic and lower eukaryotic organisms, where it is often found in transmembrane receptors that regulate two-component signaling pathways. The motif links receptor input and output modules and is essential to receptor structure and signal transduction. Rec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701832b
更新日期:2007-12-04 00:00:00
abstract::The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981072u
更新日期:1998-11-10 00:00:00
abstract::We have de novo designed a heterodimeric coiled-coil formed by two peptides as a capture/delivery system that can be used in applications such as affinity tag purification, immobilization in biosensors, etc. The two strands are designated as K coil (KVSALKE heptad sequence) and E coil (EVSALEK heptad sequence), where ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0268450
更新日期:2003-02-18 00:00:00
abstract::The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00443a008
更新日期:1989-08-22 00:00:00
abstract::Low pH-induced membrane insertion by diphtheria toxin T domain is crucial for A chain translocation into the cytoplasm. To define the membrane topography of the T domain, the exposure of biotinylated Cys residues to the cis and trans bilayer surfaces was examined using model membrane vesicles containing a deeply inser...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049354j
更新日期:2004-07-20 00:00:00
abstract::In the ras gene superfamily, codon 12 (-TGGTG-) of the K-ras gene is the most frequently mutated codon in human cancers. Recently, we have found that bulky chemical carcinogens preferentially form DNA adducts at codons 12 and 14 (-CGTAG-) in the K-ras gene in normal human bronchial epithelial (NHBE) cells. Furthermore...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034631s
更新日期:2003-08-26 00:00:00
abstract::Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035018b
更新日期:2003-10-14 00:00:00
abstract::Structures of glycopeptides obtained by exhaustive Pronase digestion of high molecular weight (1.7 X 10(5)) salmon egg polysialoglycoprotein have been elucidated. Six principal glycopeptides isolated by gel chromatography and DEAE-Sephadex A-25 chromatography in the absence or presence of borate ion were analyzed for ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00341a029
更新日期:1985-09-24 00:00:00
abstract::The left-handed polyproline II (P(II)) helix is a structure that has been given a great deal of attention lately because of its role in a wide variety of physiologically important processes and potential significance in protein unfolded states. Recent work by several authors has shown that residues besides proline can...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049922v
更新日期:2004-05-18 00:00:00
abstract::The human LD78 protein (sometimes referred to as human macrophage inflammatory protein-1 alpha) has been shown to protect multipotential hemopoietic stem cells from the effects of cytotoxic agents. Administration of the recombinant stem cell inhibitor molecule LD78 as an adjunct to chemotherapy has potential clinical ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00071a024
更新日期:1993-05-25 00:00:00
abstract::KCNE1 is a single transmembrane protein that modulates the function of voltage-gated potassium channels, including KCNQ1. Hereditary mutations in the genes encoding either protein can result in diseases such as congenital deafness, long QT syndrome, ventricular tachyarrhythmia, syncope, and sudden cardiac death. Despi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00505
更新日期:2015-10-20 00:00:00
abstract::We have studied the binding nature of an aromatic aldehyde to the catalytic site of liver alcohol dehydrogenase from horse (LADH) using preresonance Raman spectroscopy. The compound p-(dimethylamino)benzaldehyde (DABA) is converted to the corresponding alcohol in the presence of nicotinamide adenine dinucleotide (NADH...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00410a023
更新日期:1988-05-17 00:00:00
abstract::Thioacetamide, a hepatocarcinogen and an inhibitor of heme synthesis, blocks the phenobarbitone-mediated increase in the transcription of cytochrome P-450b+e messenger RNA in rat liver. This property is also shared by CoCl2 and 3-amino-1,2,4-triazole, two other inhibitors of heme synthesis. Thus, it appears feasible t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00364a009
更新日期:1986-08-12 00:00:00
abstract::In cells, RNAs likely adopt numerous intermediate conformations prior to formation of functional RNA-protein complexes. We used single-nucleotide resolution selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) to probe the structure of Escherichia coli 16S rRNA in healthy growing bacteria. SHAPE-direct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500198b
更新日期:2014-05-20 00:00:00
abstract::The aromatic hydroxylation of six pairs of selectively deuterated monosubstituted benzenes was investigated with rat liver microsomes of various induction states. The substrates studied included 3,5-D2C6H3X (1a-6a) and 2,4,6-D3C6H2X (1b-6b), where X = Br, CN, NO2, OCH3, CH3, or Ph, respectively. The deuterium content ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00308a031
更新日期:1984-06-19 00:00:00
abstract::A specific tertiary hydrogen bond that is present between the side-chain hydroxyl group of Tyr30 and the side-chain N delta 1 atom of His17 in the small, monomeric, single-domain protein, barstar, has been perturbed by site-directed mutagenesis of the sole histidine residue (His17) to a glutamine residue. The effect o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00005a027
更新日期:1995-02-07 00:00:00
abstract::alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00615a011
更新日期:1978-10-31 00:00:00
abstract::The argument and experimental evidence are presented for a two-state model that explains the action of both helical and beta-sheet antimicrobial peptides after they bind to the plasma membranes of cells. Each peptide has two distinct physical states of binding to lipid bilayers. At low peptide-to-lipid ratios (P/L), t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000946l
更新日期:2000-07-25 00:00:00
abstract::The contribution of ligand dynamics to CYP allosterism has not been considered in detail. On the basis of a previous study, we hypothesized that CYP2A6 and CYP2E1 accommodate multiple xylene ligands. As a result, the intramolecular ( k H/ k D) obs values observed for some xylene isomers are expected to be dependent on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702020y
更新日期:2008-03-04 00:00:00
abstract::In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970160w
更新日期:1997-09-23 00:00:00
abstract::A large fraction of a constitutively synthesized polypeptide, comprising 5% of the total Escherichia coli protein, is released when plasmolysed cells are subjected to osmotic shock into ice-cold water. Since the protein is not liberated by the conversion of cells to spheroplasts, it is not a typical periplasmic protei...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00656a008
更新日期:1976-06-01 00:00:00
abstract::A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9914964
更新日期:2000-01-11 00:00:00
abstract::A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00295a023
更新日期:1983-12-20 00:00:00
abstract::Myc and Max belong to the b-HLH-LZ family of transcription factors. Heterodimerization between Myc and Max or homodimerization of Max allows these proteins to bind their cognate DNA sequence known as the E-box (CACGTG). Recent evidence has suggested that the c-Myc/Max heterodimeric b-HLH-LZ could interact to form a he...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700076m
更新日期:2007-09-11 00:00:00
abstract::Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970387r
更新日期:1997-08-05 00:00:00
abstract::Saccharomyces cerevisiae tRNA and 5S RNA, Escherichia coli 5S RNA, and wheat germ 5S RNA have each been specifically spin-labeled at the 3'-terminal ribose to give morpholino-spin-labeled (MSL) RNAs. Enzymatic hydrolysis with pancreatic RNase, followed by anion-exchange chromatography, confirms the site of attachment ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00268a042
更新日期:1982-12-07 00:00:00
abstract::Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970524k
更新日期:1997-10-07 00:00:00
abstract::Numerous studies have concluded that the interaction of the amyloid β-peptide (Aβ) and cellular membranes contributes to the toxicity and cell death observed in the progression of Alzheimer's disease. Aggregated Aβ species disrupt membranes, leading to physical instability and ion leakage. Further, the presence of Aβ ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400562x
更新日期:2013-07-23 00:00:00
abstract::Stopped-flow kinetic measurements of the association of filipin with sterols in intact cells and isolated membranes of Mycoplasma capricolum were used to study the effects of varying the phospholipid in the membrane. The phospholipid composition and content of the membrane were varied by growing cells in an albumin-co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00511a019
更新日期:1981-04-14 00:00:00