Neoprontosil binding to carbonic anhydrase. Reasonance Raman and other studies on the ionization behavior of the sulfonamide.

abstract：:The cyclic decapeptide antibiotic tyrocidine A was studied by two relatively new methods, viz., correlation proton magnetic resonance (pmr) spectroscopy and double-resonance difference pmr spectroscopy. The correlation method of spectral accumulation provided pmr spectra of good resolution, and in addition the signal-...

journal_title：Biochemistry

authors： Gibbons WA,Beyer CF,Dadok J,Sprecher RF,Wyssbrod HR

更新日期：1975-01-28 00:00:00

abstract：:In the presence of Ca2+ and calmodulin (CM), purified smooth muscle myosin light chain kinase (MLCKase) was found to undergo autophosphorylation at a rate that was about 200-fold slower than its catalytic activity. Up to 1.7 mol of phosphate were incorporated per mole of kinase. Lower levels of incorporation could be ...

journal_title：Biochemistry

authors： Sobieszek A

更新日期：1995-09-19 00:00:00

abstract：:Studies are presented of the equilibrium binding of [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable binding of [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM and Kd2 = 7.7 +/- 4.6 microM, with equal numbers of binding sites. Both components are com...

journal_title：Biochemistry

authors： Neubig RR,Cohen JB

更新日期：1979-11-27 00:00:00

abstract：:Human genomic clones that span the entire protein S expressed gene (PS alpha) and the 3' two-thirds of the protein S pseudogene (PS beta) have been isolated and characterized. The PS alpha gene is greater than 80 kilobases in length and contains 14 introns and 15 exons, as well as 6 repetitive "Alu" sequences. Exons I...

journal_title：Biochemistry

authors： Schmidel DK,Tatro AV,Phelps LG,Tomczak JA,Long GL

更新日期：1990-08-28 00:00:00

abstract：:Biogenesis of pyrroloquinoline quinone (PQQ) in Klebsiella pneumoniae requires the expression of six genes (pqqA-F). One of these genes (pqqE) encodes a 43 kDa protein (PqqE) that plays a role in the initial steps in PQQ formation [Veletrop, J. S., et al. (1995) J. Bacteriol. 177, 5088-5098]. PqqE contains two highly ...

journal_title：Biochemistry

authors： Wecksler SR,Stoll S,Tran H,Magnusson OT,Wu SP,King D,Britt RD,Klinman JP

更新日期：2009-10-27 00:00:00

abstract：:Mutations in human copper-zinc superoxide dismutase (SOD1) cause an inherited form of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS). Here, we present structures of the pathogenic SOD1 variants D124V and H80R, both of which demonstrate compromised zinc-binding sites. The disruption of the zinc...

journal_title：Biochemistry

authors： Seetharaman SV,Winkler DD,Taylor AB,Cao X,Whitson LJ,Doucette PA,Valentine JS,Schirf V,Demeler B,Carroll MC,Culotta VC,Hart PJ

更新日期：2010-07-13 00:00:00

abstract：:The Fv fragment, a univalent antigen-binding unit with a molecular weight of 25,000, has successfully been prepared in high yield by limited proteolysis with clostripain of a short-chain mouse IgG2a anti-dansyl monoclonal antibody in which the entire CH1 domain is deleted [Igarashi, T., Sato, M., Takio, K., Tanaka, T....

journal_title：Biochemistry

authors： Takahashi H,Igarashi T,Shimada I,Arata Y

更新日期：1991-03-19 00:00:00

abstract：:Misaligned structures can result from strand slippage during DNA replication and, if not repaired, would lead to mutations. Previously, we showed that strand slippage can occur upon misincorporation of a dNTP opposite thymine and cytosine templates, resulting in a misaligned structure with a T- or C-bulge. The formati...

journal_title：Biochemistry

authors： Chi LM,Lam SL

更新日期：2009-12-08 00:00:00

abstract：:The reaction of ferric cytochrome c peroxidase (CcP) from Saccharomyces cerevisiae with peroxide produces compound I, characterized by both an oxyferryl iron center and a protein-based free radical. The electron paramagnetic resonance (EPR) signal of the CcP compound I radical can be resolved into a broad majority com...

journal_title：Biochemistry

authors： Fishel LA,Farnum MF,Mauro JM,Miller MA,Kraut J,Liu YJ,Tan XL,Scholes CP

更新日期：1991-02-19 00:00:00

abstract：:KLHL-12 is a substrate specific adapter protein for a Cul3-Ring ligase complex. It is a member of the Kelch β-propeller domain subclass of Cullin-Ring substrate recognition domains. This E3 ubiquitin ligase complex has many activities, including acting as a negative regulator of the Wnt signaling pathway by mediating ...

journal_title：Biochemistry

authors： Zhao B,Payne WG,Sai J,Lu Z,Olejniczak ET,Fesik SW

更新日期：2020-03-03 00:00:00

abstract：:Thermodynamic studies of bacteriorhodopsin (BR) have been undertaken in order to investigate the factors that stabilize the structure of a membrane protein. The stability of the native, intact protein was compared to that of protein with retinal removed, and/or cleaved in one or two of the loops connecting the transme...

journal_title：Biochemistry

authors： Kahn TW,Sturtevant JM,Engelman DM

更新日期：1992-09-22 00:00:00

abstract：:Fluorescent derivatives of short oligonucleotides of defined sequence were prepared by the incorporation of 5-(propylamino)uridine via current phosphoramidite chemistry, followed by derivatization of the propylamine function with mansyl chloride. These oligomers, annealed to complementary oligomers, yielded short dupl...

journal_title：Biochemistry

authors： Allen DJ,Darke PL,Benkovic SJ

更新日期：1989-05-30 00:00:00

abstract：:Membrane fusion induced by the hemagglutinin glycoprotein of influenza virus has been extensively characterized, but the mechanism whereby the protein achieves the merger of the viral and target membrane lipids remains enigmatic. Various lipid intermediate structures have been proposed, and the energies required for t...

journal_title：Biochemistry

authors： Nebel S,Bartoldus I,Stegmann T

更新日期：1995-05-02 00:00:00

abstract：:We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...

journal_title：Biochemistry

authors： Rothery RA,Weiner JH

更新日期：1996-03-12 00:00:00

abstract：:The complete sequence of histone 2B of Drosophila has been determined by using an improved Beckman sequenator. Comparing these data with those previously published by other investigators on the histone 2B of calf [Iwai, K., Hayashi, H., & Ishikawa, K. (1972) J. Biochem. (Tokyo) 72, 357--367], trout [Koostra, A., & Bai...

journal_title：Biochemistry

authors： Elgin SC,Schilling J,Hood LE

更新日期：1979-12-11 00:00:00

abstract：:The gene for the human substance P receptor (NK-1) was cloned using cDNA probes made by the polymerase chain reaction from primers based on the rat sequence. The gene spans 45-60 kb and is contained in five exons, with introns interrupting at sites homologous to those in the NK-2 receptor gene. Analysis of restriction...

journal_title：Biochemistry

authors： Gerard NP,Garraway LA,Eddy RL Jr,Shows TB,Iijima H,Paquet JL,Gerard C

更新日期：1991-11-05 00:00:00

abstract：:The thermal denaturation of caricain (the most alkaline of papain-related proteinases) was studied in acid media. Under all conditions tested, caricain denatured irreversibly following a single first-order reaction that involves simultaneous loss of secondary and tertiary structures. Besides, variation of the rate con...

journal_title：Biochemistry

authors： López-Arenas L,Solís-Mendiola S,Hernández-Arana A

更新日期：1999-11-30 00:00:00

abstract：:Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...

journal_title：Biochemistry

authors： Watson J,Nilsen-Hamilton M,Hamilton RT

更新日期：1976-04-06 00:00:00

abstract：:Many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. Recently, we showed that a single amino acid substitution in the alcohol binding pocket, L29P, in Pseudomonas fluorescens (SIK WI) aryl esterase (PFE) increased the specificity constant of P...

journal_title：Biochemistry

authors： Yin DL,Bernhardt P,Morley KL,Jiang Y,Cheeseman JD,Purpero V,Schrag JD,Kazlauskas RJ

更新日期：2010-03-09 00:00:00

abstract：:This work shows that the voltage across membranes in two very different preparations, lipid vesicles in suspension and individual HeLa cells under a microscope, is linearly related to the ratio of fluorescence excited from the two wings of the absorption spectrum of a voltage-sensitive dye. The dye di-4-ANEPPS [1-(3-s...

journal_title：Biochemistry

authors： Montana V,Farkas DL,Loew LM

更新日期：1989-05-30 00:00:00

abstract：:Light-harvesting complex II (LHC-II) regulates the light energy distribution between photosystem I and II in plants. This process is mediated by phosphorylation of the LHC-II protein, which depends on the oxidation state of photosynthetic electron carriers. In addition to this regulatory mechanism, it has recently bee...

journal_title：Biochemistry

authors： Rogl H,Kühlbrandt W,Barth A

更新日期：2003-09-02 00:00:00

abstract：:Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...

journal_title：Biochemistry

authors： Bull GD,Thompson KC

更新日期：2018-07-31 00:00:00

abstract：:The enzyme As(III) S-adenosylmethionine methyltransferase (EC 2.1.1.137) (ArsM or AS3MT) is found in members of every kingdom, from bacteria to humans. In these enzymes, there are three conserved cysteine residues at positions 72, 174, and 224 in the CmArsM orthologue from the thermophilic eukaryotic alga Cyanidioschy...

journal_title：Biochemistry

authors： Marapakala K,Qin J,Rosen BP

更新日期：2012-02-07 00:00:00

abstract：:ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with compon...

journal_title：Biochemistry

authors： Pérez-Alvarado GC,Martínez-Yamout M,Allen MM,Grosschedl R,Dyson HJ,Wright PE

更新日期：2003-06-24 00:00:00

abstract：:S100 beta is a calcium-binding protein, which regulates the activities of several enzymes and inhibits the phosphorylation of a variety of protein kinase C substrates in a calcium-dependent manner. The protein was recently found to activate a retinal membrane guanylate cyclase, and in this paper, we report that it inh...

journal_title：Biochemistry

authors： Pozdnyakov N,Margulis A,Sitaramayya A

更新日期：1998-07-28 00:00:00

abstract：:The role of histidine in catalysis by prostaglandin H synthase has been investigated using chemical modification with diethyl pyrocarbonate (DEPC), an agent that has been found to rather selectively derivatize histidine residues in proteins under mild conditions. Incubation of the synthase apoprotein with DEPC at pH 7...

journal_title：Biochemistry

authors： Zhang X,Tsai AL,Kulmacz RJ

更新日期：1992-03-10 00:00:00

abstract：:RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...

journal_title：Biochemistry

authors： Campbell LJ,Peppa M,Crabtree MD,Shafiq A,McGough NF,Mott HR,Owen D

更新日期：2015-02-17 00:00:00

abstract：:The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d62) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. Th...

journal_title：Biochemistry

authors： Driscoll DA,Samarasinghe S,Adamy S,Jonas J,Jonas A

更新日期：1991-04-02 00:00:00

abstract：:Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction of CS protein with various glycosaminoglycans in real time using surface plasmon resonance (...

journal_title：Biochemistry

authors： Rathore D,McCutchan TF,Garboczi DN,Toida T,Hernáiz MJ,LeBrun LA,Lang SC,Linhardt RJ

更新日期：2001-09-25 00:00:00

abstract：:Using the baculovirus/Sf9 cell expression system, we have incorporated 99% 15N-enriched [alpha,epsilon-15N2]-L-lysine into the rod visual pigment rhodopsin. We have subsequently investigated the protonated Schiff base (pSB) linkage in the [alpha, epsilon-15N2]Lys-rhodopsin with cross-polarization magic angle spinning ...

journal_title：Biochemistry

authors： Creemers AF,Klaassen CH,Bovee-Geurts PH,Kelle R,Kragl U,Raap J,de Grip WJ,Lugtenburg J,de Groot HJ

更新日期：1999-06-01 00:00:00