Abstract:
:The complete sequence of histone 2B of Drosophila has been determined by using an improved Beckman sequenator. Comparing these data with those previously published by other investigators on the histone 2B of calf [Iwai, K., Hayashi, H., & Ishikawa, K. (1972) J. Biochem. (Tokyo) 72, 357--367], trout [Koostra, A., & Bailey, G. S. (1978) Biochemistry 17, 2504--2510], and Patella (a limpet) [van Helden, P. D., Strickland, W. N., Brandt, W. F., & von Holt, C. (1979) Eur. J. Biochem. 93, 71--78], it is possible to assess the evolutionary stability of this protein. There is little conservation of sequence in the N-terminal portion of the molecule (residues 1--26 numbering according to calf H2B), while the remainder of the protein, which we designate the C-terminal portion, is highly conserved. In the region of 27--125 residues, there are 9 substitutions in the composite data among the 98 positions, 8 of them conservative. These data indicate that very different selective pressures operate on the two different portions of the H2B molecule, implying the existence of two well-defined regions. Studies on the structure of the nucleosome by others have suggested that the C-terminal portion of H2B is involved in histone-histone interactions while the N-terminal portion is a relatively free "tail" binding to DNA. The sequence data indicate that the function of the C-terminal region of H2B requires considerable sequence specificity while that of the N-terminal region does not.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Elgin SC,Schilling J,Hood LEdoi
10.1021/bi00592a025subject
Has Abstractpub_date
1979-12-11 00:00:00pages
5679-85issue
25eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602425c
更新日期:2007-09-18 00:00:00
abstract::Electrophilic halogenating agents, including hypohalous acids and haloamines, oxidize free methionine and the N-terminal methionines of peptides and proteins (e.g., Met-1 of anti-inflammatory peptide 1 and ubiquitin) to produce dehydromethionine (a five-membered isothiazolidinium heterocycle). Amide derivatives of met...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901343d
更新日期:2009-11-24 00:00:00
abstract::We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00582a028
更新日期:1979-07-24 00:00:00
abstract::The stereochemical problem involving a pro-pro-prochiral phosphorus center, the hydrolysis of adenosine 5'-monophosphate to adenosine and inorganic phosphate catalyzed by the venom 5'-nucleotidase, has been studied by use of chiral [16O, 17O, 18O]thiophosphates (Psi). (Rp)- and (Sp)-[alpha-18O1]Adenosine 5'-thiophosph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00564a025
更新日期:1980-11-11 00:00:00
abstract::One of the key pathological features of Alzheimer's disease is the aggregation of tau protein. We are therefore searching for compounds capable of inhibiting this reaction. On the basis of an initial screen of 200000 compounds [Pickhardt, M., Gazova, Z., von Bergen, M., Khlistunova, I., Wang, Y., Hascher, A., Mandelko...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700878g
更新日期:2007-09-04 00:00:00
abstract::The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0509402
更新日期:2005-09-27 00:00:00
abstract::The synthesis of two potential arginine-directed purine-based analogues, 6-chloro-9-(3,4-dioxopentyl)purine (6) and 9-(3,4-dioxopentyl)hypoxanthine (7), is reported. Compound 7 was extensively tested as a potential affinity label of purine nucleoside phosphorylase (EC 2.4.2.1) from human erythrocytes. Evidence that 7 ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00268a010
更新日期:1982-12-07 00:00:00
abstract::The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036269n
更新日期:2004-03-02 00:00:00
abstract::Multisite mutagenesis of single-chain and monomeric forms of human interleukin 5 (IL-5) was performed to investigate mechanistic features of receptor activation and the possibility of differentiating sites of activation from those for receptor interaction. The normally dimeric human IL-5 contains two domains, each con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001467p
更新日期:2000-12-05 00:00:00
abstract::Terminase enzymes are responsible for "packaging" of viral DNA into a preformed procapsid. Bacteriophage lambda terminase is composed of two subunits, gpA and gpNu1, in a gpA(1).gpNu1(2) holoenzyme complex. The larger gpA subunit is responsible for preparation of viral DNA for packaging, and is central to the packagin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050333e
更新日期:2005-07-19 00:00:00
abstract::Cells can be transiently permeabilized by application of high electric pulses of short duration. A direct consequence of this treatment is to induce a fusogenic state in the pulsed membrane. The molecular events underlying these phenomena remain to be explained. During our work, we investigated the effects of pulsing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00471a009
更新日期:1990-05-15 00:00:00
abstract::A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00487a005
更新日期:1990-09-04 00:00:00
abstract::The flavocytochrome p-cresol methylhydroxylase from Pseudomonas putida has been reported to have a Mr of 114,000 and to consist of two subunits, a flavoprotein and a cytochrome c, each with a Mr of 58,000. Recent X-ray crystallographic data from our laboratories [Shamala, N., Lim, L. W., Mathews, F. S., McIntire, W., ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a021
更新日期:1986-10-07 00:00:00
abstract::Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00463a032
更新日期:1990-03-20 00:00:00
abstract::We have synthesized Deltalac-acetogenins that are new acetogenin mimics possessing two n-alkyl tails without an alpha,beta-unsaturated gamma-lactone ring and suggested that their inhibition mechanism may be different from that of common acetogenins [Hamada et al. (2004) Biochemistry 43, 3651-3658]. To elucidate the in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0479003
更新日期:2005-01-18 00:00:00
abstract::Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9924258
更新日期:2000-06-20 00:00:00
abstract::The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035103j
更新日期:2003-10-28 00:00:00
abstract::The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the transition state for GPDH-catalyzed ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00116
更新日期:2016-03-15 00:00:00
abstract::CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00154
更新日期:2016-04-19 00:00:00
abstract::The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9728699
更新日期:1998-04-28 00:00:00
abstract::Heme (iron protoporphyrin IX) is an essential protein prosthetic group and signaling molecule required for most life on Earth. All heme-dependent processes require the dynamic and rapid mobilization of heme from sites of synthesis or uptake to hemoproteins present in virtually every subcellular compartment. The cytoto...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00007
更新日期:2017-04-04 00:00:00
abstract::Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972562+
更新日期:1998-05-19 00:00:00
abstract::We propose a simple method to find an amino acid sequence that is foldable into a globular protein with a desired structure based on a knowledge-based 3D-1D compatibility function. An asymmetric alpha-helical single-domain structure of sperm whale myoglobin consisting of 153 amino acid residues was chosen for the desi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi983006y
更新日期:1999-06-08 00:00:00
abstract::We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9801457
更新日期:1998-06-09 00:00:00
abstract::Transported proteins often contain an extension sequence called the signal peptide. The alkaline phosphatase (PhoA) signal sequence represents a typical signal peptide for comparison to idealized sequences both in vivo and in vitro. We have designed a series of idealized signal sequences which vary in amino terminal c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00031a012
更新日期:1995-08-08 00:00:00
abstract::Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011566i
更新日期:2002-01-22 00:00:00
abstract::Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5' terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) ribonucleic acid (RNA) to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat gern 80S ribosomes convey partial p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00551a044
更新日期:1980-05-13 00:00:00
abstract::Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00542a022
更新日期:1980-01-08 00:00:00
abstract::In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801831r
更新日期:2009-02-03 00:00:00
abstract::The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00679a018
更新日期:1975-04-22 00:00:00