Abstract:
:We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis. The junctional form of the acetylcholine receptor was purified from normal leg muscle by affinity chromatography on concanavalin A/Sepharose and cobrotoxin/Sepharose followed by sucrose gradient centrifugation. Analysis of radioiodinated receptor by polyacrylamide gel electrophoresis in sodium dodecyl sulfate indicated that the subunit structure of the junctional receptor was similar to that previously determined for the extra-junctional form (Froehner, S. C., et al. (1977) J. Biol. Chem. 252, 8589-8596), with major polypeptides, whose apparent molecular weights in 9% polyacrylamide gels were 45 000 and 51 000. In addition, several minor polypeptides were found. When the two receptors were labeled with different isotopes of iodine and run together on a sodium dodecyl sulfate gel, the subunits of one receptor could not be resolved from those of the other. As seen earlier with the extrajunctional form, the affinity alkylating reagent [3H]MBTA labeled the 45 000- and 49 000-dalton polypeptides of the junctional receptor. Peptide mapping showed that the two MBTA binding subunits are structurally related, although they are unrelated to the other polypeptides, and that the 45 000- and 51 000-dalton polypeptides of the junctional receptor were indistinguishable from those of the extrajunctional receptor. In addition, peptide mapping of the four subunits of acetylcholine receptor isolated from Torpedo californica electric organ showed that these four polypeptides appear to be structurally unrelated.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nathanson NM,Hall ZWdoi
10.1021/bi00582a028subject
Has Abstractpub_date
1979-07-24 00:00:00pages
3392-401issue
15eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The Co and Fe K-edge extended X-ray absorption fine structure (EXAFS) spectra of the methionyl aminopeptidase from Escherichia coli (EcMetAP) have been recorded in the presence of 1 and 2 equiv of either Co(II) or Fe(II) (i.e., [Co(II)_(EcMetAP)], [Co(II)Co(II)(EcMetAP)], [Fe(II)_(EcMetAP)], and [Fe(II)Fe(II)(EcMetAP)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010837m
更新日期:2001-11-06 00:00:00
abstract::The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of ortholog...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052272q
更新日期:2006-04-11 00:00:00
abstract::Carbamoyl phosphate synthetase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions occurring at three active sites connected by a molecular tunnel of 100 A. To understand the mechanism for coordination and synchronization among the active sites, the pre-steady-state time cour...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992772h
更新日期:2000-05-02 00:00:00
abstract::β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is kno...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.5b00852
更新日期:2015-10-20 00:00:00
abstract::Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00690a024
更新日期:1975-09-23 00:00:00
abstract::Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00435a014
更新日期:1989-05-02 00:00:00
abstract::A modified multiple binding equilibria treatment is presented that allows determination of thermodynamic parameters of the interaction of phospholipids with integral membrane proteins solubilized in excess detergent. Lipid binding is modeled as a series of exchange reactions between lipid molecules and detergent molec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00376a019
更新日期:1987-01-27 00:00:00
abstract::Structural and biochemical characterization of aspartate 152 at the distal heme side of catalase-peroxidase (KatG) from Synechocystis PCC 6803 reveals an important functional role for this residue. In the wild-type protein, the side chain carboxyl group of Asp152 is 7.8 A apart from the heme iron and is hydrogen-bonde...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026944d
更新日期:2003-05-13 00:00:00
abstract::A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a042
更新日期:1977-04-05 00:00:00
abstract::Horse heart cytochrome c has been modified by 8-azido-ATP and the electron-transfer activity of the modified cytochrome c's to bovine heart cytochrome c oxidase (CcO) under physiological ionic strengths has been studied by the laser flash photolysis technique with 5-deazariboflavin and EDTA as the electron donor. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00008a035
更新日期:1995-02-28 00:00:00
abstract::The cardiac and skeletal muscle sarcoplasmic reticulum ryanodine receptor Ca(2+) release channels contain thiols that are potential targets of endogenously produced reactive oxygen and nitrogen intermediates. Previously, we showed that the skeletal muscle ryanodine receptor (RyR1) has O(2)-sensitive thiols; only when ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8012627
更新日期:2008-12-30 00:00:00
abstract::Glucocorticoid receptors of wild-type and nti ("increased nuclear transfer") mutant S49.1 mouse lymphoma cells exist in extracts under low-salt conditions predominantly as high molecular weight species (Mr greater than or equal to 300,000). These receptor-hormone complexes are unable to bind to DNA. High salt (300 mM ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00415a030
更新日期:1988-07-26 00:00:00
abstract::The gene for human pancreatic cholesterol esterase consists of 11 exons and 10 introns and is 9.2 kb in length. The last and longest exon (841 nucleotides) is unique to the human gene. Functional amino acids are encoded on separate exons. The leader sequence is encoded by a single exon which carries two additional N-t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00141a017
更新日期:1992-07-07 00:00:00
abstract::We have investigated the occurrence of cyclic electron flow in intact spinach leaves. In particular, we have tested the hypothesis that cyclic flow requires the presence of supercomplexes in the thylakoid membrane or other strong associations between proteins. Using biochemical approaches, we found no evidence of the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061439s
更新日期:2006-11-14 00:00:00
abstract::The structure of empty capsids of Kilham rat virus, an autonomous parvovirus with icosahedral symmetry, was investigated by small-angle neutron scattering. From the forward scatter, the molecular weight was determined to be 4.0 X 10(6), and from the Guinier region, the radius of gyration was found to be 105 A in D2O a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a044
更新日期:1984-12-18 00:00:00
abstract::It has been found that centrifugation of (ethylenediamine)tetraacetic acid dissociated L-cell polyribosomes at 25 degrees C through preformed CS2SO4 density gradients containing 15% dimethyl sulfoxide resolves them into two distinct classes of particles that contain ribosomal and messenger RNA, respectively. Ribosomal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00661a019
更新日期:1976-08-10 00:00:00
abstract::Thermodynamic parameters of helix formation were measured spectroscopically for 16 oligoribonucleotides containing either internal GU mismatches or the corresponding AU pairs. Internal GU mismatches stabilize each helix, but not as much as the corresponding AU pairs. The differences in the enthalpy and entropy changes...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00367a061
更新日期:1986-09-23 00:00:00
abstract::The binding of a ligand to cell surface receptors initiates a cascade of intracellular signals that generate responses to the external stimuli. Thus, this event plays a pivotal role in the mechanism of transmembrane signaling. Activin is a member of a cytokine family that is involved in diverse biological processes. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981939o
更新日期:1998-11-24 00:00:00
abstract::We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a003
更新日期:1991-02-19 00:00:00
abstract::Aureochromes have recently been shown to act as blue-light-regulated transcription factors in the stramenopile alga Vaucheria frigida. They comprise a light-, oxygen-, or voltage-sensitive (LOV) domain as a sensory module with flavin mononucleotide (FMN) as a chromophore and a basic region leucine zipper (bZIP) domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400197u
更新日期:2013-05-07 00:00:00
abstract::Folylpoly-gamma-glutamate synthetase (FPGS) is the enzyme responsible for metabolic trapping of reduced folate cofactors in cells for use in nucleotide and amino acid biosynthesis. There are two isoforms of FPGS expressed in mouse tissues, one is expressed in differentiated tissue, principally liver and kidney, and th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015644d
更新日期:2002-01-08 00:00:00
abstract::Superoxide reductase (SOR) is an Fe protein that catalyzes the reduction of superoxide to give H(2)O(2). Recently, the mutation of the Glu47 residue into alanine (E47A) in the active site of SOR from Desulfoarculus baarsii has allowed the stabilization of an iron-peroxo species when quickly reacted with H(2)O(2) [Math...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0498151
更新日期:2004-07-13 00:00:00
abstract::We have characterized a membrane protein containing residues P688-T762 of the integrin beta3 subunit, encompassing its transmembrane and cytoplasmic domains, by nuclear magnetic resonance spectroscopy. Under conditions in which it is monomeric in dodecylphosphocholine micelles, the protein consists mainly of alpha-hel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026822l
更新日期:2002-12-31 00:00:00
abstract::The GlmU protein is a bifunctional enzyme with both acetyltransferase and uridylyltransferase (pyrophosphorylase) activities which catalyzes the transformation of glucosamine-1-P, UTP, and acetyl-CoA to UDP-N-acetylglucosamine [Mengin-Lecreulx, D., & van Heijenoort, J. (1994) J. Bacteriol. 176, 5788-5795], a fundament...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952275a
更新日期:1996-01-16 00:00:00
abstract::The mechanism by which acyl-CoA dehydrogenases initiate catalysis was studied by using p-substituted phenylacetyl-CoAs (substituents-NO2, -CN, and CH3CO-), 3S-C8-, and 3'-dephospho-3S-C8CoA. These analogues lack a beta C-H and cannot undergo alpha,beta-dehydrogenation. Instead they deprotonate at alpha C-H at pH > or ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971827h
更新日期:1998-02-17 00:00:00
abstract::Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9528659
更新日期:1996-07-30 00:00:00
abstract::Anaerobic induction of light harvesting and reaction center gene expression involves two transacting factors termed RegA and RegB. Sequence and mutational analysis has indicated that RegA and RegB constitute cognate components of a prokaryotic sensory transduction cascade with RegB comprising a membrane-spanning senso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00002a002
更新日期:1995-01-17 00:00:00
abstract::The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00267a039
更新日期:1982-11-23 00:00:00
abstract::Polymerase eta is a member of the Y family of DNA polymerases which is able to bypass thymine dimers efficiently and in a relatively error-free manner. To elucidate the mechanism of dimer bypass, the efficiency of dAMP and pyrene nucleotide insertion opposite the thymine dimer and its N3-methyl derivatives was determi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0345687
更新日期:2003-08-12 00:00:00
abstract::Peptidyl derivatives of diphenyl (alpha-aminoalkyl)phosphonates have been synthesized and are effective and specific inhibitors of serine proteases at low concentrations. Z-PheP(OPh)2 irreversibly reacts with chymotrypsin (kobsd/[I] = 1200 M-1 s-1) and does not react with two elastases. The best inhibitor for most chy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00216a026
更新日期:1991-01-15 00:00:00