Pyridoxal 5'-phosphate and analogs as probes of coenzyme-protein interaction in Baccillus alvei tryptophanase.

abstract：:We have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with [3H]tamoxifen aziridine [(...

journal_title：Biochemistry

authors： Katzenellenbogen BS,Elliston JF,Monsma FJ Jr,Springer PA,Ziegler YS,Greene GL

更新日期：1987-04-21 00:00:00

abstract：:The RmFixL-RmFixJ oxygen signal transduction system ensures that a cascade of the Sinorhizobium meliloti nitrogen fixation genes is induced as the concentration of O2 drops below 50 microM in symbiotic nodules. Deoxy-RmFixL is a histidine protein kinase that catalyzes a phosphoryl transfer from ATP to the aspartate 54...

journal_title：Biochemistry

authors： Sousa EH,Gonzalez G,Gilles-Gonzalez MA

更新日期：2005-11-22 00:00:00

abstract：:Site-directed mutagenesis has been used to construct three recombinant mutant hemoglobins (rHbs), rHb(beta L105W), rHb(alpha D94A/betaL105W), and rHb(alpha D94A). rHb(beta L105W) is designed to form a new hydrogen bond from beta 105Trp to alpha 94Asp in the alpha(1)beta(2) subunit interface to lower the oxygen binding...

journal_title：Biochemistry

authors： Fang TY,Simplaceanu V,Tsai CH,Ho NT,Ho C

更新日期：2000-11-14 00:00:00

abstract：:The mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involving primer 3' OH deprotonation, nucleophilic attack, and pyrophosphate leaving-group elimination, preceded by dNTP binding which induces a large-amplitude conformational change for Watson-Crick nascent base pairs. Ambi...

journal_title：Biochemistry

authors： Sucato CA,Upton TG,Kashemirov BA,Osuna J,Oertell K,Beard WA,Wilson SH,Florián J,Warshel A,McKenna CE,Goodman MF

更新日期：2008-01-22 00:00:00

abstract：:Human cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) belongs to a family of closely related proteins found in mammals and reptiles. Some mammalian CRISPs are known to be involved in the process of reproduction, whereas some of the CRISPs from reptiles are neurotoxin-like substances found in lizard sa...

journal_title：Biochemistry

authors： Udby L,Sørensen OE,Pass J,Johnsen AH,Behrendt N,Borregaard N,Kjeldsen L

更新日期：2004-10-12 00:00:00

abstract：:Retroviruses contain a dimeric RNA consisting of two identical molecules of genomic RNA. The interaction between the two monomers is thought to occur near their 5'ends. We previously identified a region upstream from the splice donor site, comprising an autocomplementary sequence, responsible for the formation of dime...

journal_title：Biochemistry

authors： Muriaux D,Fossé P,Paoletti J

更新日期：1996-04-16 00:00:00

abstract：:The [2Fe-2S] cluster of the Rieske iron-sulfur protein is held between two loops of the protein that are connected by a disulfide bridge. We have replaced the two cysteines that form the disulfide bridge in the Rieske protein of Saccharomyces cerevisiae with tyrosine and leucine, and tyrosine and valine, to evaluate t...

journal_title：Biochemistry

authors： Merbitz-Zahradnik T,Zwicker K,Nett JH,Link TA,Trumpower BL

更新日期：2003-11-25 00:00:00

abstract：:Phosphorus-31 magic angle sample spinning NMR spectra have been employed to investigate the structure and composition of the mineral deposits in chicken bone. Three different pulse sequences, Bloch decay, cross-polarization, and dipolar suppression, were employed to obtain spectra from bone specimens of varying age. T...

journal_title：Biochemistry

authors： Roufosse AH,Aue WP,Roberts JE,Glimcher MJ,Griffin RG

更新日期：1984-12-04 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:We have examined the DNA damage produced by reaction of peroxyl radicals with human fibroblast DNA. DNA damage consisted of both strand breaks and base modifications. The extent of strand breaks and base modifications induced as a function of peroxyl radical concentration was determined by quantitation of fragment siz...

journal_title：Biochemistry

authors： Rodriguez H,Valentine MR,Holmquist GP,Akman SA,Termini J

更新日期：1999-12-14 00:00:00

abstract：:The EcDos protein belongs to a group of heme-based sensors that detect their ligands with a heme-binding PAS domain. Among these various heme-PAS proteins, EcDos is unique in having its heme iron coordinated at both axial positions to residues of the protein. To achieve its high affinities for ligands, one of the axia...

journal_title：Biochemistry

authors： Gonzalez G,Dioum EM,Bertolucci CM,Tomita T,Ikeda-Saito M,Cheesman MR,Watmough NJ,Gilles-Gonzalez MA

更新日期：2002-07-02 00:00:00

abstract：:The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

journal_title：Biochemistry

authors： Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

更新日期：1990-06-19 00:00:00

abstract：:Among the 118 genes upregulated by Pseudomonas aeruginosa in response to iron starvation [Ochsner, U. A., Wilderman, P. J., Vasil, A. I., and Vasil, M. L. (2002) Mol. Microbiol. 45, 1277-1287], we focused on the products of the two genes encoding electron transfer proteins, as a means of identifying the redox partners...

journal_title：Biochemistry

authors： Wang A,Zeng Y,Han H,Weeratunga S,Morgan BN,Moënne-Loccoz P,Schönbrunn E,Rivera M

更新日期：2007-10-30 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:We have recently described a procedure for the purification of microtubule associated protein 1B (MAP1B) from calf brain [Pedrotti, B., & Islam K. (1995) Cell Motil. Cytoskeleton 30, 301-309], and this study further characterizes the purified protein and its interaction with microtubules. We show that purified MAP1B (...

journal_title：Biochemistry

authors： Pedrotti B,Ulloa L,Avila J,Islam K

更新日期：1996-03-05 00:00:00

abstract：:Treatment of ADP-glucose pyrophosphorylase (EC 2.7.7.27) from the cyanobacterium Anabaena PCC 7120 with phenylglyoxal in 50 mM Hepes, pH 8.0, at 25 degrees C resulted in a time- and concentration-dependent loss of enzyme activity. Phosphate, the inhibitor, protected the enzyme from inactivation most effectively, while...

journal_title：Biochemistry

authors： Sheng J,Preiss J

更新日期：1997-10-21 00:00:00

abstract：:2,3-Butanedione monoxime (BDM) reversibly inhibits force production in muscle. At least part of its action appears to be directly on the contractile apparatus. To understand better its mechanism of action, we studied the effect of BDM on the steps of myosin subfragment 1 Mg(2+)-ATPase in 0.1 M potassium acetate, pH 7....

journal_title：Biochemistry

authors： Herrmann C,Wray J,Travers F,Barman T

更新日期：1992-12-08 00:00:00

abstract：:Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...

journal_title：Biochemistry

authors： Czekster CM,Naismith JH

更新日期：2017-04-18 00:00:00

abstract：:The amino acid sequence of the peptide alpha1(I)-CB6B, the carboxyl-terminal peptide containing 91 residues of alpha1(I) chain of chick skin collagen, is described. The amino acid sequence was determined by automated Edman degradation of intact peptide plus its tryptic and thermolytic peptides. The comparison of this ...

journal_title：Biochemistry

authors： Dixit SN,Seyer JM,Kang AH,Gross J

更新日期：1978-12-26 00:00:00

abstract：:The effects of metal ion binding on the optical spectroscopic properties and temperature stability of two single tryptophan mutants of chicken skeletal TnC, F78W and F154W, have been examined. The absence of tyrosine and other tryptophan residues allowed the unambiguous assignment of the spectral signal from the intro...

journal_title：Biochemistry

authors： Moncrieffe MC,Venyaminov SY,Miller TE,Guzman G,Potter JD,Prendergast FG

更新日期：1999-09-14 00:00:00

abstract：:Mdm2 negatively regulates p53 by inhibiting its transcriptional activity and promoting its degradation by functioning as an E3 ubiquitin ligase. The primary p53 binding site on mdm2 is located in its N-terminal domain. Through binding to p53 at its N-terminal transactivation domain, mdm2 directly blocks the transcript...

journal_title：Biochemistry

authors： Ma J,Martin JD,Zhang H,Auger KR,Ho TF,Kirkpatrick RB,Grooms MH,Johanson KO,Tummino PJ,Copeland RA,Lai Z

更新日期：2006-08-01 00:00:00

abstract：:We have systematically validated the activity and inhibition of a HIV-1 protease (PR) variant bearing 17 mutations (PR(S17)), selected to represent high resistance by machine learning on genotype-phenotype data. Three of five mutations in PR(S17) correlating with major drug resistance, M46L, G48V, and V82S, and five o...

journal_title：Biochemistry

authors： Park JH,Sayer JM,Aniana A,Yu X,Weber IT,Harrison RW,Louis JM

更新日期：2016-04-26 00:00:00

abstract：:Integrins are one of the major mediators of cellular adherence. Structurally the component alpha and beta chains are characterized by extensive intrachain disulfide bonding. The assignment of these bonds is currently based on homology with the chains of the integrin alphaIIbbeta3. However, recent crystallographic anal...

journal_title：Biochemistry

authors： Krokhin OV,Cheng K,Sousa SL,Ens W,Standing KG,Wilkins JA

更新日期：2003-11-11 00:00:00

abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...

journal_title：Biochemistry

authors： van Oort MG,Dijkman R,Hille JD,de Haas GH

更新日期：1985-12-31 00:00:00

abstract：:Recently, a new clathrin assembly protein (AP180) has been purified from coated vesicles of bovine brain (Ahle & Ungewickell, 1986). This protein has been shown to promote polymerization of clathrin into a homogeneous population of baskets under conditions where pure clathrin does not polymerize by itself. We have pur...

journal_title：Biochemistry

authors： Prasad K,Lippoldt RE

更新日期：1988-08-09 00:00:00

abstract：:A new fluorescent lipid analogue, bimanephosphatidylcholine, has been synthesized for use in lipid bilayers. This probe is well suited as an energy-transfer donor with N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine as the acceptor. Dimyristoylphosphatidylethanolamine vesicles are prepared by sonication a...

journal_title：Biochemistry

authors： Pryor C,Bridge M,Loew LM

更新日期：1985-04-23 00:00:00

abstract：:Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e.,...

journal_title：Biochemistry

authors： Rucker RB,Heng-Khoo CS,Dubick M,Lefevre M,Cross CE

更新日期：1979-09-04 00:00:00

abstract：:Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain. To facilitate large-scale purification of the SecYEG heterotrimer, SecY was fused at its amino terminus with a hexahistidine tag and co-overexpressed with SecE and Sec...

journal_title：Biochemistry

authors： van der Does C,Manting EH,Kaufmann A,Lutz M,Driessen AJ

更新日期：1998-01-06 00:00:00

abstract：:Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as par...

journal_title：Biochemistry

authors： Chu LK,Huang WY,Littledike ET,Hamilton JW,Cohn DV

更新日期：1975-08-12 00:00:00

abstract：:Translesion DNA synthesis represents the ability of a DNA polymerase to misinsert a nucleotide opposite a DNA lesion. Previous kinetic studies of the bacteriophage T4 DNA polymerase using a series of non-natural nucleotides suggest that pi-electron density of the incoming nucleotide substantially contributes to the ef...

journal_title：Biochemistry

authors： Zhang X,Donnelly A,Lee I,Berdis AJ

更新日期：2006-11-07 00:00:00