Abstract:
:Evidence is presented that indicates tropoelastin is derived from a soluble elastin with a molecular weight of 95000. Tropoelastin and its proposed precursor were isolated from the aortas of copper-deficient chicks. Although it is doubtful that the proposed precursor is an initial product of elastin translation, i.e., a proelastin, it is proposed to be at least a truncated form of proelastin that is converted to tropoelastin. The key to its isolation was the presence of alpha 1-antitrypsin at each step in the purification procedure. The first 11 amino acid residues at the NH2 terminal of the proposed tropoelastin precursor (GGVPGVAVPGGV) are the same as those for tropoelastin. Its amino acid composition is similar to that of tropoelastin, except for higher amounts of acidic amino acid residues. Further, the proposed precursor contains a limited number of aldehydic functions, presumably in the form of peptidyl allysine. This was taken as an indication that the proposed precursor serves as a substract for lysyl oxidase. Under the conditions used for the isolation, the precursor appeared to be in higher concentrations than tropoelastin in aorta extracts from copper-deficient chicks.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rucker RB,Heng-Khoo CS,Dubick M,Lefevre M,Cross CEdoi
10.1021/bi00585a004subject
Has Abstractpub_date
1979-09-04 00:00:00pages
3854-9issue
18eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9805036
更新日期:1998-10-06 00:00:00
abstract::The highly processive motor, myosin V, has an extremely long neck containing six calmodulin-binding IQ motifs that allows it to take multiple 36 nm steps corresponding to the pseudo-repeat of actin. To further investigate how myosin V moves processively on actin filaments, we altered the length of the neck by adding o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0512086
更新日期:2005-12-13 00:00:00
abstract::The activity of peptidyl-tRNALys-CpCp2'dA was measured in an in vitro poly(A)-dependent polypeptide synthesizing system derived from Escherichia coli. It has already been shown that Lys-tRNALys-CpCp2'dA is active as an acceptor and Ac2-Lys-tRNALys-Cp2'dA can donate its peptidyl residue but that the overall poly(A)-dep...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00270a013
更新日期:1983-01-04 00:00:00
abstract::Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000637v
更新日期:2000-10-31 00:00:00
abstract::The binding of heparin to high molecular weight kininogen (H-kininogen) was analyzed by the effect of kininogen in decreasing the heparin-induced enhancement of the rate of inactivation of thrombin by antithrombin. The conditions were arranged so that the heparin-catalyzed antithrombin-thrombin reaction, monitored in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00429a039
更新日期:1989-02-07 00:00:00
abstract::The peptides generated from the degradation of the oxidized B chain of bovine insulin by the multiproteinase complex macropain (proteasome) have been analyzed by reverse-phase peptide mapping and identified by N-terminal amino acid sequencing and composition analysis. Six of the 29 peptide bonds in the insulin B chain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00224a022
更新日期:1991-03-12 00:00:00
abstract::The photoaffinity probes [gamma-32P]2-azidoATP (2-N3ATP) and [alpha-32P]8-azido-ATP (8-N3ATP) were used to investigate the binding of ATP to highly purified 2-5A synthetase. 2-N3ATP and 8-N3ATP are substrates for 2-5A synthetase [Suhadolnik, R.J., Karikó, K., Sobol, R.W., Jr., Li, S.W., Reichenbach, N.L., & Haley, B.E...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00424a024
更新日期:1988-11-29 00:00:00
abstract::The synthesis of 5-(2-oxalylethyl)-NADH, a reduced nicotinamide adenine dinucleotide (NADH) derivate with pyruvate covalently attached to the 5 position of the dihydronicotinamide ring over an additional methylene group has been described previously (Trommer, W.E., Blume, H., and Kapmeyer, H. (1976) Justus Liebigs Ann...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00668a012
更新日期:1976-11-16 00:00:00
abstract::In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond ( journal_title:Biochemistry pub_type: 杂志文章 doi:10.1021/bi700904a 更新日期:2007-10-30 00:00:00
abstract::We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9805947
更新日期:1998-11-10 00:00:00
abstract::A mutant form of the nitrogenase iron protein with a deletion of residue Leu 127, located in the switch II region of the nucleotide binding site, forms a tight, inactive complex with the nitrogenase molybdenum iron (MoFe) protein in the absence of nucleotide. The structure of this complex generated with proteins from ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001645e
更新日期:2001-01-23 00:00:00
abstract::PhnZ utilizes a mixed valence diiron(II/III) cofactor and O2 to oxidatively cleave the carbon-phosphorus bond of (R)-2-amino-1-hydroxyethylphosphonic acid to form glycine and orthophosphate. The active site residues Y24 and E27 are proposed to mediate induced-fit recognition of the substrate and access of O2 to one of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00145
更新日期:2019-12-31 00:00:00
abstract::As discussed in the accompanying paper [Markland, W., Ley, A. C., & Ladner, R. C. (1996) Biochemistry 35, 8045-8057], we generated libraries from the first Kunitz domain of human lipoprotein-associated coagulation inhibitor (LACI-D1) using multivalent M13 III display and derived potent inhibitors of human plasmin (PLA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952629y
更新日期:1996-06-18 00:00:00
abstract::Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are different both in sequence and in structure from known chromosomal DHFRs. These plasmid-derived DHFRs are responsible for conferring trimethoprim resistance to the host strain. A derivative of R388 DHFR, RBG200, has been cloned and overp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00494a011
更新日期:1990-10-23 00:00:00
abstract::Rat mucosal-type mast cells of the RBL-2H3 line express a glycoprotein termed the MAst cell Function-associated Antigen (MAFA). When MAFA is clustered by its specific monoclonal antibody G63, secretion normally triggered by aggregating these cells' type I Fcepsilon receptor (FcepsilonRI) is substantially inhibited. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011566i
更新日期:2002-01-22 00:00:00
abstract::Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8002956
更新日期:2008-07-08 00:00:00
abstract::The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1016793
更新日期:2011-01-11 00:00:00
abstract::The respiratory chain complexes can arrange into multienzyme assemblies, so-called supercomplexes. We present the first 3D map of a respiratory chain supercomplex. It was determined by random conical tilt electron microscopy analysis of a bovine supercomplex consisting of complex I, dimeric complex III, and complex IV...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700983h
更新日期:2007-11-06 00:00:00
abstract::Clostridial neurotoxins are zinc endopeptidases, and each contains one Zn(2+)/molecule. To investigate the structural/functional role of Zn(2+) in botulinum neurotoxin light chain (the enzymatic subunit of the neurotoxin), the effect of the removal of zinc on protein folding and enzyme kinetics was investigated. The a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0007472
更新日期:2000-08-29 00:00:00
abstract::The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00526a028
更新日期:1981-11-10 00:00:00
abstract::The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00632a012
更新日期:1977-06-28 00:00:00
abstract::The (1)H NMR resonances of the heme substituents of the low-spin Fe(III) form of nitrophorin 2, as its complexes with N-methylimidazole (NP2-NMeIm) and imidazole (NP2-ImH), have been assigned by a combination of (1)H homonuclear two-dimensional NMR techniques and (1)H-(13)C HMQC. Complete assignment of the proton and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026765w
更新日期:2003-01-28 00:00:00
abstract::M13 DNA containing 20-30 apurinic/apyrimidinic (AP) sites per intact circular molecule was prepared by growing phage on an ung- dut- Escherichia coli mutant and treating the DNA with uracil N-glycosylase. AP sites obstruct in vitro DNA synthesis catalyzed by E. coli pol I. The position at which termination of synthesi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00288a026
更新日期:1983-09-13 00:00:00
abstract::Leucine aminopeptidase (LAP) is a protease requiring two divalent metal cations per subunit for activity. Zn2+, Mg2+, and Co2+ metal-substituted forms of LAP have been prepared and investigated kinetically. Substitution of metal into the two binding sites independently resulted in the preparation of Zn2+Zn2+, Mg2+Zn2+...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00285a010
更新日期:1983-08-02 00:00:00
abstract::The effects of solution variables on the in vitro reconstitution of calf brain tubulin, purified by the method of Weisenberg et al. (Weisenberg, R. C., Borisy, G. G., and Taylor, E. W. (1968), Biochemistry 7, 4466-4479; Weisenberg, R. C., and Timasheff, S. N. (1970), Biochemistry 9, 4110-4116), as modified by Lee et a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00627a037
更新日期:1977-04-19 00:00:00
abstract::Human erythrocyte spectrin prepared from fresh blood is a mixture of different association states. Depending on the manner of preparation, the two-chain dimer or the tetramer predominates. These forms are not in rapid thermodynamic equilibrium. The molecular weight of the dimer by sedimentation and diffusion and by li...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00644a028
更新日期:1977-12-13 00:00:00
abstract::The subgel phases of a homologous series of saturated straight-chain diacylphosphatidylcholines with hydrocarbon chains consisting of 10-18 carbon atoms were studied by Fourier-transform infrared spectroscopy. All of these lipids initially form a subgel phase which is spectroscopically similar to that obtained when fu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00486a024
更新日期:1990-08-28 00:00:00
abstract::This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015828s
更新日期:2002-01-29 00:00:00
abstract::Myosins are a superfamily of actin-binding motor proteins with significant variations in kinetic properties (such as actin binding affinity) between different isoforms. It remains unknown how such kinetic variations arise from the structural and dynamic tuning of the actin-myosin interface at the amino acid residue le...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi4006896
更新日期:2013-11-26 00:00:00
abstract::Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050595g
更新日期:2005-09-20 00:00:00