Abstract:
:Integrins are one of the major mediators of cellular adherence. Structurally the component alpha and beta chains are characterized by extensive intrachain disulfide bonding. The assignment of these bonds is currently based on homology with the chains of the integrin alphaIIbbeta3. However, recent crystallographic analysis of the soluble alphaVbeta3 construct indicates that the alphaV chain displays bonding patterns different from those predicted for alphaIIb. In an effort to define the disulfide bonding patterns in integrins, we have used mass spectrometric based approaches to map the human alpha3, alpha5, alphaV, and alphaIIb. The results indicate that there are differences in the disulfide patterns of the alpha chains. These do not correlate with the integrin capacity to bind ligands as all integrins used in the present study displayed functional activity. The differences were observed in the bonding patterns linking the heavy (H) and light (L) components of the of the alpha chains. It was also possible to assign the location in alpha5 of an additional disulfide bond involving a pair of cysteines not present in alphaV or alphaIIb. This second bond between the H and L chains of alpha5 has not been previously described. These results indicate that not all integrin species display the same disulfide bonding patterns. They also highlight the need for caution in the use of assignments based on sequence homology.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Krokhin OV,Cheng K,Sousa SL,Ens W,Standing KG,Wilkins JAdoi
10.1021/bi034726usubject
Has Abstractpub_date
2003-11-11 00:00:00pages
12950-9issue
44eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The kinetics of self-association for beef liver glutamate dehydrogenase (EC 1.4.1.3) have been measured by using pressure perturbation in both the time domain and the frequency domain by monitoring scattered light intensity. The kinetic behavior is entirely consistent with the random self-association model proposed by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00579a007
更新日期:1979-06-12 00:00:00
abstract::A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00436a010
更新日期:1989-05-16 00:00:00
abstract::p-Cresol methylhydroxylase (PCMH) isolated from Pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit Mr 49,000 and 9,000. It is a flavocytochrome c containing covalently bound FAD in the larger subunit and covalently bound heme in the smaller. Crystals in space group P2(1)2(1)2(1) with unit-cell par...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00215a034
更新日期:1991-01-08 00:00:00
abstract::Hemophores from Serratia marcescens (HasA(sm)) and Pseudomonas aeruginosa (HasA(p)) bind hemin between two loops, which harbor the axial ligands H32 and Y75. Hemin binding to the Y75 loop triggers closing of the H32 loop and enables binding of H32. Because Yersinia pestis HasA (HasA(yp)) presents a Gln at position 32,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400280z
更新日期:2013-04-23 00:00:00
abstract::When they are translated, steroid receptors are assembled into a multiprotein complex containing hsp90, p23, an immunophilin, and often some hsp70. Some of the receptors, such as that for progesterone, have nuclear localization signals that are functional in the absence of hormone, and they move into the nucleus where...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970648x
更新日期:1997-06-24 00:00:00
abstract::The group-specific antigen was isolated from a type Ia group B streptococcal strain and is a complex polysaccharide composed of alpha-L-rhamnopyranosyl, alpha-D-galactopyranosyl, 2-acetamido-2-deoxy-beta-D-glucopyranosyl, D-glucitol, and phosphate residues. The complexity of the group B polysaccharide antigen is evide...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00376a020
更新日期:1987-01-27 00:00:00
abstract::The involvement of calmodulin in the proliferation of Chinese hamster embryo fibroblast cells has been studied with a specific monoclonal antibody to calmodulin. We observed that calmodulin levels increase 2-fold in the late G1 period in these cells, and this coincides with the increase in DNA polymerase alpha activit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00158a002
更新日期:1992-11-03 00:00:00
abstract::Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201095z
更新日期:2011-09-06 00:00:00
abstract::Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resist...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971276v
更新日期:1997-10-21 00:00:00
abstract::The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00055a002
更新日期:1993-02-02 00:00:00
abstract::Known crystal structures of class II aminoacyl-tRNA synthetases complexed to their cognate tRNAs reveal that critical acceptor stem contacts are made by the variable loop connecting the beta-strands of motif 2 located within the catalytic core of class II synthetases. To identify potential acceptor stem contacts made ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001835p
更新日期:2000-12-19 00:00:00
abstract::Gp is a major GTP-binding protein of human placenta and platelets [Evans, T., Brown, M. L., Fraser, E. D., & Northup, J. K. (1986) J. Biol. Chem. 261, 7052-7059]. High-affinity guanine nucleotide binding is associated with a polypeptide migrating identically with H-ras on SDS-PAGE. We have characterized the interactio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00482a002
更新日期:1990-07-31 00:00:00
abstract::We investigated the effects of substituting two of the four tryptophans (the "inner pair" Trp(9) and Trp(11) or the "outer pair" Trp(13) and Trp(15)) in gramicidin A (gA) channels. The conformational preferences of the doubly substituted gA analogues were assessed using circular dichroism spectroscopy and size-exclusi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200136e
更新日期:2011-06-07 00:00:00
abstract::Helicases are enzymes that use energy from nucleoside triphosphate hydrolysis to unwind double-stranded (ds) DNA, a process vital to virtually every phase of DNA metabolism. Helicases have been classified as either 5'-to-3' or 3'-to-5' on the basis of their ability to unwind duplex DNA adjacent to either a 5' or 3' si...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi012058b
更新日期:2002-02-19 00:00:00
abstract::The interaction of the TEM-2 beta-lactamase with 9-deoxyclavulanic acid (3) and with both extensively labeled (2) and specifically labeled (1) clavulanic acid has been studied. The close similarity between 9-doexyclavulanate and clavulanate in kinetics, spectroscopic, and protein chemical terms show that the allyl alc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00514a035
更新日期:1981-05-26 00:00:00
abstract::Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00567a017
更新日期:1980-12-23 00:00:00
abstract::Biochemical analysis of a rat fibroblast cell clone, transfected with the murine Ia associated invariant chain gene, demonstrates the expression of a family of proteins. This indicates that all members of the invariant protein family are derived from the same gene. The proteins p41, Ii, p27, p25, and p10, synthesized ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00411a028
更新日期:1988-05-31 00:00:00
abstract::Calmodulin is a ubiquitous calcium dependent protein. In the presence of calcium, calmodulin adopts an altered conformation that leads to the generation of downstream cellular calcium signals. Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. These probe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8014295
更新日期:2009-01-27 00:00:00
abstract::Transcarboxylase (TC) is a multisubunit enzyme that catalyzes the transfer of a carboxylate group from methylmalonyl-CoA (MMCoA) to pyruvate. The CO2- group is shuttled between the MMCoA and pyruvate binding sites by a biotin cofactor, covalently linked to the 1.3S subunit. Fully carboxylated 1.3S can be prepared in v...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0116442
更新日期:2002-02-19 00:00:00
abstract::Lysyl-5-fluoro-L-tryptophyllysine and lysyl-5-fluoro-D-tryptophyllysine were synthesized, and their interactions with double-stranded DNA were investigated as a model for protein-nucleic acid interactions. The binding to DNA was studied by monitoring various 19F NMR parameters, the fluorescence, and the optical absorb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00337a012
更新日期:1985-07-30 00:00:00
abstract::Several polypeptide toxins from sea anemones caused delayed inactivation of mammalian voltage-dependent sodium channels, resulting in a positive inotropic effect on the heart. Anthopleurin B (ApB), a toxin produced by the sea anemone Anthopleura xanthogrammica, is the most potent of all known anemone toxins. Previous ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9528457
更新日期:1996-03-19 00:00:00
abstract::The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a016
更新日期:1977-05-03 00:00:00
abstract::F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034746w
更新日期:2003-10-07 00:00:00
abstract::A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00509a022
更新日期:1981-03-17 00:00:00
abstract::A description of the efficient high-level expression of the monomer hemoglobin (GMG4) from Glycera dibranchiata is presented. The cDNA described by Simons and Satterlee [Simons, P.C., & Satterlee, J.D. (1989) Biochemistry 28, 8525-8530] was subcloned into an expression system, and conditions were found that led to the...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00200a014
更新日期:1994-08-30 00:00:00
abstract::Urea-induced unfolding of Na,K-ATPase from pig kidney and from shark salt gland was studied by electron paramagnetic resonance (EPR) spectroscopy of a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups which are essential for activity. Urea-induced structural changes lead to the inhibition of Na...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901124j
更新日期:2009-09-29 00:00:00
abstract::Comparison of available sequences of HLA-A and HLA-B antigens shows that variable positions are predominantly localized in four segments spanning residues 63-85, 105-116, 138-156, and 177-194. The fourth segment is unique in that it contains no differences between antigens of the same locus. Secondary folding of HLA h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00300a007
更新日期:1984-02-28 00:00:00
abstract::The three-dimensional structure of the fatty-acid-binding protein isolated from the flight muscle of the desert locust Schistocerca gregaria has been solved and refined to a crystallographic R-value of 18.5% for all measured X-ray data from 30.0- to 2.2-A resolution. Crystals employed in the investigation were grown f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00207a004
更新日期:1994-10-18 00:00:00
abstract::Escherichia coli were grown on 14.3% uniformly 13C-labeled glucose as the sole carbon source and challenged anaerobically with 90% 13C-labeled formaldehyde. The major multiply labeled metabolites were identified by 13C NMR spectroscopy to be glycerol and 1,2-propanediol, and a minor metabolite was shown to be 1,3-prop...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a050
更新日期:1985-07-16 00:00:00
abstract::Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00239a002
更新日期:1991-06-25 00:00:00