Preparation of the Fv fragment from a short-chain mouse IgG2a anti-dansyl monoclonal antibody and use of selectively deuterated Fv analogues for two-dimensional 1H NMR analyses of the antigen-antibody interactions.

abstract：:The effects of several divalent cations, various polybasic amines, and lysozyme on the rate of polymerization of 8S clathrin to the 300S coat structure have been evaluated by turbidimetric procedures. Ca2+ and Mn2+ strongly enhance the rate of polymerization. Only spermine among the naturally occurring polybasic amine...

journal_title：Biochemistry

authors： Nandi PK,Van Jaarsveld PP,Lippoldt RE,Edelhoch H

更新日期：1981-11-10 00:00:00

abstract：:The structures of synthetic peptides corresponding to regions of putative secondary structure in the cellular prion protein PrPC were studied as models for the conformational transition that features in the formation of the pathogenic isoform, PrPSc. Transgenetic studies argue that these PrP isoforms interact during t...

journal_title：Biochemistry

authors： Nguyen J,Baldwin MA,Cohen FE,Prusiner SB

更新日期：1995-04-04 00:00:00

abstract：:Chemical modification of Torpedo californica acetylcholinesterase by various sulfhydryl reagents results in its conversion to one of two principal states. One of these states, viz., that produced by disulfides and alkylating agents, is stable. The second state, produced by mercury derivatives, is metastable. At room t...

journal_title：Biochemistry

authors： Kreimer DI,Dolginova EA,Raves M,Sussman JL,Silman I,Weiner L

更新日期：1994-12-06 00:00:00

abstract：:CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...

journal_title：Biochemistry

authors： Pappas A,Park TS,Carman GM

更新日期：1999-12-14 00:00:00

abstract：:We have examined proteins containing the DNA-binding domain of c-Myb with biophysical methods. This DNA-binding domain consists of three imperfect repeats (R1, R2, and R3) conserved among many species. Our results indicate that the DNA-binding domain forms unspecific and specific complexes with oligodeoxynucleotides. ...

journal_title：Biochemistry

authors： Ebneth A,Schweers O,Thole H,Fagin U,Urbanke C,Maass G,Wolfes H

更新日期：1994-12-06 00:00:00

abstract：:Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

journal_title：Biochemistry

authors： Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

更新日期：2014-06-10 00:00:00

abstract：:von Willebrand A domain-containing protein 8 (VWA8) is a poorly characterized, mitochondrial matrix-targeted protein with an AAA ATPase domain and ATPase activity that increases in livers of mice fed a high-fat diet. This study was undertaken to use CRISPR/Cas9 to delete VWA8 in cultured mouse hepatocytes and gain ins...

journal_title：Biochemistry

authors： Luo M,Willis WT,Coletta DK,Langlais PR,Mengos A,Ma W,Finlayson J,Wagner GR,Shi CX,Mandarino LJ

更新日期：2019-12-10 00:00:00

abstract：:Cystathionine gamma-synthase (CGS) is a pyridoxal phosphate-dependent enzyme that catalyzes a gamma-replacement reaction, in which the succinyl group of an O-succinyl-L-homoserine (L-OSHS) is displaced by the thiol of L-cysteine to form L-cystathionine, in the first step of the bacterial transsulfuration pathway. The ...

journal_title：Biochemistry

authors： Aitken SM,Kim DH,Kirsch JF

更新日期：2003-09-30 00:00:00

abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...

journal_title：Biochemistry

authors： Sigfridsson K,Young S,Hansson O

更新日期：1996-01-30 00:00:00

abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

journal_title：Biochemistry

authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

更新日期：2014-04-22 00:00:00

abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...

journal_title：Biochemistry

authors： Ishihara K,Suzuki J,Nagata S

更新日期：2016-06-14 00:00:00

abstract：:Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...

journal_title：Biochemistry

authors： Mitchell EP,Withers SG,Ermert P,Vasella AT,Garman EF,Oikonomakos NG,Johnson LN

更新日期：1996-06-11 00:00:00

abstract：:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric ...

journal_title：Biochemistry

authors： Turell L,Botti H,Carballal S,Ferrer-Sueta G,Souza JM,Durán R,Freeman BA,Radi R,Alvarez B

更新日期：2008-01-08 00:00:00

abstract：:The growth and shortening of microtubules in guanosine triphosphate-(GTP-) mediated dynamic instability has previously been observed to occur at rates which are remarkably variable (Gildersleeve et al., 1992, Chrétien et al., 1995). Neighboring microtubules observed simultaneously can grow or shorten at different rate...

journal_title：Biochemistry

authors： Dye RB,Williams RC Jr

更新日期：1996-11-12 00:00:00

abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...

journal_title：Biochemistry

authors： Mozer TJ,Tiemeier DC,Jaworski EG

更新日期：1983-03-01 00:00:00

abstract：:In iron-deficient conditions, Pseudomonas aeruginosa secretes a major fluorescent siderophore named pyoverdin (Pvd), which after chelating iron(III) is transported back into the cell via its outer membrane receptor FpvA. FpvA is a TonB-dependent transport protein and has the ability to bind Pvd in its apo- or iron-loa...

journal_title：Biochemistry

authors： Clément E,Mesini PJ,Pattus F,Schalk IJ

更新日期：2004-06-22 00:00:00

abstract：:The human pathogen Staphylococcus aureus acquires heme iron from hemoglobin (Hb) via the action of a series of iron-regulated surface determinant (Isd) proteins. The cell wall anchored IsdB protein is recognized as the predominant Hb receptor, and is comprised of two NEAr transporter (NEAT) domains that act in concert...

journal_title：Biochemistry

authors： Fonner BA,Tripet BP,Eilers BJ,Stanisich J,Sullivan-Springhetti RK,Moore R,Liu M,Lei B,Copié V

更新日期：2014-06-24 00:00:00

abstract：:The relaxin-like factor (RLF) also named insulin-like 3 (INSL3) consists of two polypeptide chains linked by two interchain and one intrachain disulfide bond. RLF binds to its receptor (LGR8 also named RXFP2) through the B chain and initiates transmembrane communication by activating the adenylate cyclase through the ...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：2012-05-22 00:00:00

abstract：:Group VIA phospholipase A(2) (iPLA(2)beta) is expressed in phagocytes, vascular cells, pancreatic islet beta-cells, neurons, and other cells and plays roles in transcriptional regulation, cell proliferation, apoptosis, secretion, and other events. A bromoenol lactone (BEL) suicide substrate used to study iPLA(2)beta f...

journal_title：Biochemistry

authors： Song H,Bao S,Ramanadham S,Turk J

更新日期：2006-05-23 00:00:00

abstract：:To gain insight into the folding of large proteins, we constructed a bovine beta-lactoglobulin (beta-lg) dimeric mutant, A34C/C121A beta-lg. In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer's inte...

journal_title：Biochemistry

authors： Yagi M,Kameda A,Sakurai K,Nishimura C,Goto Y

更新日期：2008-06-03 00:00:00

abstract：:Peptide NH resonances in the 250 MHZ 1H nuclear magnetic resonance (NMR) spectrum of oxytocin in H2O were assigned to specific amino acid residues by the "underwater decoupling" technique (i.e., decoupling from corresponding CalphaH resonances, which are buried beneath the intense water peak). These experiments confir...

journal_title：Biochemistry

authors： Glickson JD,Rowan R,Pitner TP,Dadok J,Bothner-By AA,Walter R

更新日期：1976-03-09 00:00:00

abstract：:In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis ...

journal_title：Biochemistry

authors： Dreveny I,Andryushkova AS,Glieder A,Gruber K,Kratky C

更新日期：2009-04-21 00:00:00

abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...

journal_title：Biochemistry

authors： Christensen T,Svensson B,Sigurskjold BW

更新日期：1999-05-11 00:00:00

abstract：:Halogen bonding is a weak chemical force that has so far mostly found applications in crystal engineering. Despite its potential for use in drug discovery, as a new molecular tool in the direction of molecular recognition events, it has rarely been assessed in biopolymers. Motivated by this fact, we have developed a p...

journal_title：Biochemistry

authors： Danelius E,Andersson H,Jarvoll P,Lood K,Gräfenstein J,Erdélyi M

更新日期：2017-06-27 00:00:00

abstract：:Protein flexibility is thought to play key roles in numerous biological processes, including antibody affinity maturation, signal transduction, and enzyme catalysis, yet only limited information is available regarding the molecular details linking protein dynamics with function. A single point mutation at the distal s...

journal_title：Biochemistry

authors： Grossman M,Tworowski D,Dym O,Lee MH,Levy Y,Murphy G,Sagi I

更新日期：2010-07-27 00:00:00

abstract：:Light-induced hypsochromic shifts of the Q(y) absorption band of the bacteriochlorophyll dimer (P) from 865 to 850 nm were identified using continuous illumination of dark-adapted reaction centers (RCs) from Rhodobacter capsulatus when dispersed in the most commonly used detergent, the zwitterionic lauryl N-dimethylam...

journal_title：Biochemistry

authors： Deshmukh SS,Akhavein H,Williams JC,Allen JP,Kalman L

更新日期：2011-06-14 00:00:00

abstract：:Voltage-dependent L-type calcium (Ca) channels are heteromultimeric proteins that are regulated through phosphorylation by cAMP-dependent protein kinase (PKA). We demonstrated that the beta 2 subunit was a substrate for PKA in intact cardiac myocytes through back-phosphorylation experiments. In addition, a heterologou...

journal_title：Biochemistry

authors： Gerhardstein BL,Puri TS,Chien AJ,Hosey MM

更新日期：1999-08-10 00:00:00

abstract：:The backbone dynamics of Escherichia coli ribonuclease HI (RNase HI) in the picosecond to nanosecond time scale were characterized by a combination of measurements of 15N-NMR relaxation (T1, T2, and NOE), analyzed by a model-free approach, and molecular dynamics (MD) simulation in water. The MD simulations in water we...

journal_title：Biochemistry

authors： Yamasaki K,Saito M,Oobatake M,Kanaya S

更新日期：1995-05-23 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free fragment there was a previous proposal according to which it would fold into a stable helical structure forming a dimer at concentrations above 0.06 mM. A complete...

journal_title：Biochemistry

authors： Rico M,Jiménez MA,González C,De Filippis V,Fontana A

更新日期：1994-12-13 00:00:00