Prion protein peptides induce alpha-helix to beta-sheet conformational transitions.

abstract：:Nucleotide excision repair (NER) is a crucial pathway in the maintenance of genome stability requiring at least two dozen proteins. XPA and RPA have essential roles in the damage recognition step of NER. To better understand the mechanism of their interactions with DNA, we utilized equilibrium and stop-flow kinetic ap...

journal_title：Biochemistry

authors： Iakoucheva LM,Walker RK,van Houten B,Ackerman EJ

更新日期：2002-01-08 00:00:00

abstract：:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...

journal_title：Biochemistry

authors： Varticovski L,Chahwala SB,Whitman M,Cantley L,Schindler D,Chow EP,Sinclair LK,Pepinsky RB

更新日期：1988-05-17 00:00:00

abstract：:Adeno-associated virus (AAV) nonstructural proteins Rep78 and Rep68 carry out all DNA transactions that regulate the AAV life cycle. They share two multifunctional domains: an N-terminal origin binding/nicking domain (OBD) from the HUH superfamily and a SF3 helicase domain. A short linker of ∼20 amino acids that is cr...

journal_title：Biochemistry

authors： Musayev FN,Zarate-Perez F,Bardelli M,Bishop C,Saniev EF,Linden RM,Henckaerts E,Escalante CR

更新日期：2015-09-29 00:00:00

abstract：:Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...

journal_title：Biochemistry

authors： Khananshvili D,Baazov D,Weil-Maslansky E,Shaulov G,Mester B

更新日期：1996-12-10 00:00:00

abstract：:Depletion of Ca2+ and/or Cl- ions from PSII membranes blocks the electron-transfer reactions that precede O2 evolution on the oxidizing side of the enzyme. Illumination of these inhibited preparations at 273 K generates a paramagnetic species that is detectable by low-temperature (T < 20 K) EPR as a signal in the g = ...

journal_title：Biochemistry

authors： Lydakis-Simantiris N,Dorlet P,Ghanotakis DF,Babcock GT

更新日期：1998-05-05 00:00:00

abstract：:The effects of various compounds such as the transport substrate ADP and the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA) on the labeling of cysteine residues in the ADP/ATP carrier of bovine heart submitochondrial particles by the SH reagent eosin-5-maleimide (EMA) were studied. Of the f...

journal_title：Biochemistry

authors： Majima E,Shinohara Y,Yamaguchi N,Hong YM,Terada H

更新日期：1994-08-16 00:00:00

abstract：:DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...

journal_title：Biochemistry

authors： Yang Y,Qin Y,Ding Q,Bakhtina M,Wang L,Tsai MD,Zhong D

更新日期：2014-08-26 00:00:00

abstract：:Among hormone-inducible transcription factors, estrogen receptors (ERs) play important roles in tissue growth and differentiation, via either direct or indirect binding, in the nucleus, to specific DNA targets called estrogen responsive elements (EREs), or through nongenomic pathways. In humans, two estrogen receptor ...

journal_title：Biochemistry

authors： Le Grand A,André-Leroux G,Marteil G,Duval H,Sire O,Le Tilly V

更新日期：2015-06-30 00:00:00

abstract：:Oligomerization of numerous G protein-coupled receptors has been documented, including the prototypic family B secretin receptor. The clinical significance of oligomerization of this receptor became clear with the recent observation that a misspliced form present in pancreatic cancer could associate with the wild-type...

journal_title：Biochemistry

authors： Lisenbee CS,Miller LJ

更新日期：2006-07-11 00:00:00

abstract：:The aggregation of the protein tau into amyloid fibrils is known to be involved in the causation of the neurodegenerative tauopathies and the progression of cognitive decline in Alzheimer's disease. This review surveys the mechanism of tau aggregation with special emphasis on the information obtained from biochemical ...

journal_title：Biochemistry

authors： Ramachandran G,Udgaonkar JB

更新日期：2013-06-18 00:00:00

abstract：:The VWF A1 domain seems to possess two heparin binding regions (residues 565-587 and 633-648) displaying positively charged amino acids, but the overall polyanion-A1 domain interaction scheme remains essentially elusive. To probe this molecular reaction as well as the role of electrostatic forces in VWF-heparin intera...

journal_title：Biochemistry

authors： Rastegar-Lari G,Villoutreix BO,Ribba AS,Legendre P,Meyer D,Baruch D

更新日期：2002-05-28 00:00:00

abstract：:The 86-amino acid colicin E9 immunity protein (Im9), which inhibits the DNase activity of colicin E9, has been overexpressed in Escherichia coli and isotopically enriched with 15N and 13C. Using the 3D CBCANH and CBCA(CO)NH experiments, we have almost completely assigned the backbone 13C resonances and extended previo...

journal_title：Biochemistry

authors： Osborne MJ,Breeze AL,Lian LY,Reilly A,James R,Kleanthous C,Moore GR

更新日期：1996-07-23 00:00:00

abstract：:Tec family nonreceptor tyrosine kinases are key immunological enzymes that control processes that range from T and B cell development to reorganization of the actin cytoskeleton. The full-length Tec kinases have been resistant to crystallization. This lack of structural data and the paucity of in vitro biochemical dat...

journal_title：Biochemistry

authors： Joseph RE,Min L,Andreotti AH

更新日期：2007-05-08 00:00:00

abstract：:Activated receptors with a tyrosine kinase activity induce a variety of responses like changes in the differentiation and mitogenic status of cells. These responses are mediated in part by p21ras. Some of these activated receptors induce in certain cell types a pronounced, but transient, increase in Ras-GTP. We have s...

journal_title：Biochemistry

authors： Osterop AP,Medema RH,Ouwens DM,Van der Zon GC,Möller W,Maassen JA

更新日期：1994-06-14 00:00:00

abstract：:Escherichia coli asparagine synthetase B (AS-B) catalyzes the synthesis of asparagine from aspartate, glutamine, and ATP. A combination of kinetic, isotopic-labeling, and stoichiometry studies have been performed to define the nature of nitrogen transfer mediated by AS-B. The results of initial rate studies were consi...

journal_title：Biochemistry

authors： Boehlein SK,Stewart JD,Walworth ES,Thirumoorthy R,Richards NG,Schuster SM

更新日期：1998-09-22 00:00:00

abstract：:The mechanism of oxidation of a peptide substrate by the flavoprotein lysine-specific demethylase (LSD1) has been examined using the effects of pH and isotopic substitution on steady-state and rapid-reaction kinetic parameters. The substrate contained the 21 N-terminal residues of histone H3, with a dimethylated lysyl...

journal_title：Biochemistry

authors： Gaweska H,Henderson Pozzi M,Schmidt DM,McCafferty DG,Fitzpatrick PF

更新日期：2009-06-16 00:00:00

abstract：:A new procedure to cleave tryptophanyl peptide bonds in high yield is reported. The method involves treatment of the S-alkylated protein with o-iodosobenzoic acid. The procedure is highly selective for tryptophan and does not modify tyrosine or histidine, but may convert methionine to its sulfoxide derivative. The yie...

journal_title：Biochemistry

authors： Mahoney WC,Hermodson MA

更新日期：1979-08-21 00:00:00

abstract：:The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...

journal_title：Biochemistry

authors： Pioszak AA,Ninfa AJ

更新日期：2003-07-29 00:00:00

abstract：:The electrophoretic mobilities of 24 single-stranded DNA oligomers, each containing 26 nucleotide residues, have been measured in polyacrylamide gels and in free solution. The mobilities observed at 20 degrees C differed by approximately 20% in polyacrylamide gels and by approximately 10% in free solution, even though...

journal_title：Biochemistry

authors： Stellwagen E,Abdulla A,Dong Q,Stellwagen NC

更新日期：2007-09-25 00:00:00

abstract：:Mistic is an unusual membrane protein from Bacillus subtilis. It appears to fold and insert autonomously into a lipid bilayer and has been suggested as a tool that aids the targeting of eukaryotic membrane proteins to bacterial membranes. The NMR structure of Mistic in detergent (LDAO) micelles has revealed it to be a...

journal_title：Biochemistry

authors： Psachoulia E,Bond PJ,Sansom MS

更新日期：2006-08-01 00:00:00

abstract：:The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...

journal_title：Biochemistry

authors： Fodor E,Mack JW,Maeng JS,Ju JH,Lee HS,Gruschus JM,Ferretti JA,Ginsburg A

更新日期：2005-09-20 00:00:00

abstract：:A series of truncated forms of His(6)-tagged gp91phox were expressed, solubilized, and purified in the presence of 30 microM FAD. The truncated gp91phox with the longest sequence in the C-terminal region (221-570) (gp91C) showed the highest activity (turnover rate, 0.92) for NADPH diaphorase in the presence of either ...

journal_title：Biochemistry

authors： Nisimoto Y,Ogawa H,Miyano K,Tamura M

更新日期：2004-07-27 00:00:00

abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...

journal_title：Biochemistry

authors： Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

更新日期：2004-01-13 00:00:00

abstract：:11Beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) catalyzes the conversion of 11-dehydrocorticosterone to its active form corticosterone in rodents (or cortisone to cortisol in humans). The reductive reaction of the 11-keto to 11-hydroxyl is the pivotal switch in the activation of glucocorticoids. An excess of ...

journal_title：Biochemistry

authors： Zhang J,Osslund TD,Plant MH,Clogston CL,Nybo RE,Xiong F,Delaney JM,Jordan SR

更新日期：2005-05-10 00:00:00

abstract：:alpha-Conotoxin GI is a snail toxin protein consisting of 13 amino acids cross-linked by 2 intramolecular disulfide bridges. This toxin is an antagonist of acetylcholine receptors. The native sequence has been synthesized, along with nine additional variants in which non-cysteine residues are replaced by alanine or th...

journal_title：Biochemistry

authors： Zhang RM,Snyder GH

更新日期：1991-11-26 00:00:00

abstract：:31P NMR spectra of equilibrium mixtures of enzyme-bound reactants and products of the adenylate kinase reaction (formula; see text) were analyzed by using computer simulations based on density matrix theory of chemical exchange. Since adenylate kinase has the unique feature that the reactants in the reverse direction ...

journal_title：Biochemistry

authors： Vasavada KV,Kaplan JI,Nageswara Rao BD

更新日期：1984-02-28 00:00:00

abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...

journal_title：Biochemistry

authors： Treves S,Scutari E,Robert M,Groh S,Ottolia M,Prestipino G,Ronjat M,Zorzato F

更新日期：1997-09-23 00:00:00

abstract：:JZTX-XI is a peptide toxin isolated from the venom of the Chinese spider Chilobrachys jingzhao. It contains 34 residues including six cysteine residues with disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Using 3'- and 5'-RACE methods, the full-length cDNA was identified as encoding an 86-residue pr...

journal_title：Biochemistry

authors： Liao Z,Yuan C,Deng M,Li J,Chen J,Yang Y,Hu W,Liang S

更新日期：2006-12-26 00:00:00

abstract：:The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Fra...

journal_title：Biochemistry

authors： Ho C,Shanmugasundararaj S,Miller KW,Malinowski SA,Cook AC,Slater SJ

更新日期：2008-09-09 00:00:00

abstract：:The effect of Ca2+ binding on the circular dichroism (CD) and 270-MHz proton nuclear magnetic resonance (NMR) spectra of brain-specific S-100b calcium binding protein has been examined at two pH values, 8.5 and 7.5. At pH 8.5, S-100b protein binds two Ca2+ per monomer with Kd values of 6 x 10(-5) and 2 x 10(-4) M, whe...

journal_title：Biochemistry

authors： Mani RS,Shelling JG,Sykes BD,Kay CM

更新日期：1983-03-29 00:00:00