Solution structure and functional characterization of jingzhaotoxin-XI: a novel gating modifier of both potassium and sodium channels.

abstract：:Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...

journal_title：Biochemistry

authors： Furter R,Snaith M,Gillespie DE,Hall BD

更新日期：1992-11-10 00:00:00

abstract：:DNA-DNA interstrand cross-links are thought to be important for the cytotoxicity of many chemotherapeutic agents. To study this more definitively, adduct site-specific methods are used to construct a plasmid with a single nitrogen mustard interstrand cross-link (inter-HN2-pTZSV28). Replication efficiency (RE = [coloni...

journal_title：Biochemistry

authors： Berardini M,Mackay W,Loechler EL

更新日期：1997-03-25 00:00:00

abstract：:Vanadate can often bind to phosphoryl transfer enzymes to form a trigonal-bipyramidal structure at the active site. The enzyme-vanadate dissociation constants in these enzymes are much lower than those for phosphate. Therefore, enzyme-bound vanadate moieties are often considered as transition state analogues. To test ...

journal_title：Biochemistry

authors： Deng H,Callender R,Huang Z,Zhang ZY

更新日期：2002-05-07 00:00:00

abstract：:The Pseudomonas aeruginosa FpvA receptor is a TonB-dependent outer membrane transport protein that catalyzes uptake of ferric pyoverdin across the outer membrane. Surprisingly, FpvA expressed in P. aeruginosa grown in an iron-deficient medium copurifies with a ligand X that we have characterized by UV, fluorescence, a...

journal_title：Biochemistry

authors： Schalk IJ,Kyslik P,Prome D,van Dorsselaer A,Poole K,Abdallah MA,Pattus F

更新日期：1999-07-20 00:00:00

abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...

journal_title：Biochemistry

authors： Sulkes M,Lewis A,Marcus MA

更新日期：1978-10-31 00:00:00

abstract：:Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...

journal_title：Biochemistry

authors： Arnold U,Schulenburg C,Schmidt D,Ulbrich-Hofmann R

更新日期：2006-03-21 00:00:00

abstract：:Alpha-sarcin is an exquisitely specific ribonuclease that binds and cleaves a single phosphodiester bond in the large rRNA of the eukaryotic ribosome, inactivating it. To better understand this remarkable activity, the contributions of the active site residues (His 50, Glu 96, and His 137) to the conformational stabil...

journal_title：Biochemistry

authors： García-Mayoral MF,del Pozo AM,Campos-Olivas R,Gavilanes JG,Santoro J,Rico M,Laurents DV,Bruix M

更新日期：2006-11-21 00:00:00

abstract：:Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...

journal_title：Biochemistry

authors： Miles HT,Frazier J

更新日期：1978-07-11 00:00:00

abstract：:Microfibrils having a diameter of 10-12 nm, found either in association with elastin or independently, are an important component of the extracellular matrix of many tissues. Because isolation of native proteins composing the microfibrils has proven difficult, information on structure/function relationships is limited...

journal_title：Biochemistry

authors： Bashir MM,Abrams WR,Rosenbloom J,Kucich U,Bacarra M,Han MD,Brown-Augsberger P,Mecham R,Rosenbloom J

更新日期：1994-01-18 00:00:00

abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...

journal_title：Biochemistry

authors： Ernst RJ,Komor AC,Barton JK

更新日期：2011-12-20 00:00:00

abstract：:NMR spectroscopy is particularly adept at site-specifically monitoring dynamic processes in proteins, such as protein folding, domain movements, ligand binding, and side-chain rotations. By coupling the favorable spectroscopic properties of highly dynamic side-chain methyl groups with transverse-relaxation-optimized s...

journal_title：Biochemistry

authors： Boswell ZK,Latham MP

更新日期：2019-01-22 00:00:00

abstract：:Separation of the gradient-purified gastric microsome into two membrane subfractions of distinct enzymatic and phospholipid composition has been achieved by mild SDS (0.033% w/v) treatment followed by sucrose gradient centrifugation of the pig and rabbit gastric microsomes. While the high-density membranes had all of ...

journal_title：Biochemistry

authors： Ray TK,Das PK,Nandi J,Banerjee A,Bandopadhyay S

更新日期：1988-12-13 00:00:00

abstract：:Holliday junctions are central intermediates in the process of genetic recombination. These DNA molecules contain four double helices that flank a central branch point, so that each of the four strands that constitute the junction is associated with two different helices. Previously, we have shown that such branched j...

journal_title：Biochemistry

authors： Wang H,Seeman NC

更新日期：1995-01-24 00:00:00

abstract：:The effects of poly(ethylene glycol) (PEG) on sonicated unilamellar vesicles made of phosphatidylcholine have been examined. Stopped-flow and equilibrium data are presented for vesicle aggregation, vesicle leakage, lipid mixing, and aqueous contents mixing. Vesicle aggregation is detected as a monoexponential increase...

journal_title：Biochemistry

authors： Viguera AR,Mencía M,Goñi FM

更新日期：1993-04-13 00:00:00

abstract：:A type II topoisomerase is essential for decatenating DNA replication products, and it accomplishes this task by passing one DNA duplex through a transient break in a second duplex. The B' domain of topoisomerase II contains three highly conserved motifs, EGDSA, PL(R/K)GK(I/L/M)LNVR, and IMTD(Q/A)DXD. We have investig...

journal_title：Biochemistry

authors： West KL,Meczes EL,Thorn R,Turnbull RM,Marshall R,Austin CA

更新日期：2000-02-15 00:00:00

abstract：:The betagamma-crystallin superfamily consists of evolutionarily related proteins with domain topology similar to lens beta- and gamma-crystallins, formed from duplicated Greek key motifs. Ca(2+) binding was found in a few betagamma-crystallin members earlier, although its prevalence and diversity as inherent molecular...

journal_title：Biochemistry

authors： Aravind P,Mishra A,Suman SK,Jobby MK,Sankaranarayanan R,Sharma Y

更新日期：2009-12-29 00:00:00

abstract：:Traditionally, assigning the heme protein resonances has relied heavily on the comparison of spectra arising from protein reconstituted with specifically deuterated hemes and the native form. Such an approach can identify tentatively the broad, overlapping signals in the Fe(II) high-spin heme protein spectra. Although...

journal_title：Biochemistry

authors： Busse SC,Jue T

更新日期：1994-09-13 00:00:00

abstract：:Reductive dehalogenation such as that catalyzed by iodotyrosine deiodinase (IYD) is highly unusual in aerobic organisms but necessary for iodide salvage from iodotyrosine generated during thyroxine biosynthesis. Equally unusual is the dependence of this process on flavin. Rapid kinetics have now been used to define th...

journal_title：Biochemistry

authors： Bobyk KD,Ballou DP,Rokita SE

更新日期：2015-07-28 00:00:00

abstract：:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...

journal_title：Biochemistry

authors： Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

更新日期：2009-06-16 00:00:00

abstract：:A 150-kDa DNA-dependent ATPase composed of 83/68-kDa subunits was previously reported to cofractionate with a 21S complex of enzymes for DNA synthesis from HeLa cells (Vishwanatha, J. K., & Baril, E. F. (1990) Biochemistry 29, 8753-8759). The DNA-dependent ATPase was purified to electrophoretic homogeneity from a HeLa...

journal_title：Biochemistry

authors： Cao QP,Pitt S,Leszyk J,Baril EF

更新日期：1994-07-19 00:00:00

abstract：:The micellar properties of mixed, bovine gangliosides and purified galactosyl-N-acetylacetylgalactosaminyl (N-acetylneuraminyl) galactosylglucosylceramide were studied by gel filtration, equilibrium dialysis, and band and boundary centrifugation in sucrose gradients. The dissociation of micelles is very slow (days) in...

journal_title：Biochemistry

authors： Formisano S,Johnson ML,Lee G,Aloj SM,Edelhoch H

更新日期：1979-03-20 00:00:00

abstract：:Because of its ability to selectively glucosylate misfolded glycoproteins, UDP-glucose:glycoprotein glucosyltransferase (UGGT) functions as a folding sensor in the glycoprotein quality control system in the endoplasmic reticulum (ER). The unique property of UGGT derives from its ability to transfer a glucose residue t...

journal_title：Biochemistry

authors： Ohara K,Takeda Y,Daikoku S,Hachisu M,Seko A,Ito Y

更新日期：2015-08-11 00:00:00

abstract：:The VWF A1 domain seems to possess two heparin binding regions (residues 565-587 and 633-648) displaying positively charged amino acids, but the overall polyanion-A1 domain interaction scheme remains essentially elusive. To probe this molecular reaction as well as the role of electrostatic forces in VWF-heparin intera...

journal_title：Biochemistry

authors： Rastegar-Lari G,Villoutreix BO,Ribba AS,Legendre P,Meyer D,Baruch D

更新日期：2002-05-28 00:00:00

abstract：:The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...

journal_title：Biochemistry

authors： Eder J,Kirschner K

更新日期：1992-04-14 00:00:00

abstract：:The nontransformed steroid receptors contain several non-steroid binding proteins, such as hsp90, hsp70, and p59. Recently, we and others have shown that p59 (FKBP59) is an immunophilin which binds two potent immunosuppressants, FK506 and rapamycin. This raises the possibility that FK506 or rapamycin may modify the fu...

journal_title：Biochemistry

authors： Tai PK,Albers MW,McDonnell DP,Chang H,Schreiber SL,Faber LE

更新日期：1994-09-06 00:00:00

abstract：:Site-directed mutations R177A and R177K in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium were generated. The mutant enzymes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase gene promoter, puri...

journal_title：Biochemistry

authors： Sollewijn Gelpke MD,Moënne-Loccoz P,Gold MH

更新日期：1999-08-31 00:00:00

abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...

journal_title：Biochemistry

authors： Parr RD,Martin GG,Hostetler HA,Schroeder ME,Mir KD,Kier AB,Ball JM,Schroeder F

更新日期：2007-07-17 00:00:00

abstract：:We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...

journal_title：Biochemistry

authors： Kloc M,Li XX,Etkin LD

更新日期：1993-08-17 00:00:00

abstract：:The aureolic acid antitumor antibiotic mithramycin (MTM) inhibits both cancer growth and bone resorption by cross-linking GC-rich DNA, thus blocking binding of Sp-family transcription factors to gene regulatory elements. Transcription of c-src, a gene implicated in many human cancers and required for osteoclast-depend...

journal_title：Biochemistry

authors： Remsing LL,Bahadori HR,Carbone GM,McGuffie EM,Catapano CV,Rohr J

更新日期：2003-07-15 00:00:00

abstract：:Two peptides designed for drug delivery were generated by the combination of a signal peptide with a nuclear localization sequence and are shown to facilitate the cellular internalization of small molecules which are covalently linked to these peptides. In order to understand the mechanism of internalization, the conf...

journal_title：Biochemistry

authors： Chaloin L,Vidal P,Heitz A,Van Mau N,Méry J,Divita G,Heitz F

更新日期：1997-09-16 00:00:00