Copurification of the FpvA ferric pyoverdin receptor of Pseudomonas aeruginosa with its iron-free ligand: implications for siderophore-mediated iron transport.

abstract：:Ligand-induced dimerization of receptor tyrosine kinases (RTKs) modulates a system of linked biochemical reactions, sharply switching the RTK from a quiescent state to an active state that becomes phosphorylated and triggers intracellular signaling pathways. To improve our understanding of this molecular switch, we de...

journal_title：Biochemistry

authors： Sheth PR,Hays JL,Elferink LA,Watowich SJ

更新日期：2008-04-01 00:00:00

abstract：:Detailed 31P NMR measurements have been conducted on pig platelet dense granules and aqueous mixtures of ATP, ADP, MgCl2, and 5HT. The resonance line widths of the dense granule nucleotides were temperature independent above approximately 30 degrees C; below this temperature they exhibit a strong temperature dependenc...

journal_title：Biochemistry

authors： Uğurbil K,Fukami MH,Holmsen H

更新日期：1984-01-31 00:00:00

abstract：:The membrane topology model of the Na(+)-citrate transporter CitS of Klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. A split version of CitS was constructed to study domain interactions and proximity relationships of...

journal_title：Biochemistry

authors： Dobrowolski A,Fusetti F,Lolkema JS

更新日期：2010-06-01 00:00:00

abstract：:Activation of the protein tyrosine kinase receptors requires the coupling of ligand binding to a change in both the proximity and orientation of the single transmembrane (TM) helices of receptor monomers to allow transphosphorylation of the receptor kinase domain. We make use of peptides corresponding to the TM and ju...

journal_title：Biochemistry

authors： Tamagaki H,Furukawa Y,Yamaguchi R,Hojo H,Aimoto S,Smith SO,Sato T

更新日期：2014-08-05 00:00:00

abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...

journal_title：Biochemistry

authors： Miyao-Tokutomi M,Inoue Y

更新日期：1992-01-21 00:00:00

abstract：:The third and most recently identified Parkinson's disease-linked variant of the neuronal protein alpha-synuclein to be identified (E46K) results in widespread brain pathology and early onset Parkinson symptoms (Zarranz et al. (2004) Ann. Neurol. 55, 164-173). Herein, we present biochemical and biophysical characteriz...

journal_title：Biochemistry

authors： Fredenburg RA,Rospigliosi C,Meray RK,Kessler JC,Lashuel HA,Eliezer D,Lansbury PT Jr

更新日期：2007-06-19 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:Cross-linking of antibodies constitutes a widespread initiation signal for their respective effector functions. Cross-linking IgE-class antibodies provide the triggering signal to mast cells for their degranulation process. To obtain a quantitative insight into these cross-linking processes, the interactions between a...

journal_title：Biochemistry

authors： Schweitzer-Stenner R,Licht A,Lüscher I,Pecht I

更新日期：1987-06-16 00:00:00

abstract：:Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...

journal_title：Biochemistry

authors： Shearman MS,Beher D,Clarke EE,Lewis HD,Harrison T,Hunt P,Nadin A,Smith AL,Stevenson G,Castro JL

更新日期：2000-08-01 00:00:00

abstract：:Prolyl hydroxylase is a glycoprotein containing two nonidentical subunits, alpha and beta. The alpha subunit of prolyl hydroxylase isolated from 13-day-old chick embryos contains a single high mannose oligosaccharide having seven mannosyl residues. Two forms of alpha subunit have been shown to exist in enzyme purified...

journal_title：Biochemistry

authors： Kedersha NL,Tkacz JS,Berg RA

更新日期：1985-10-08 00:00:00

abstract：:The fluorescence of Eu(III) is used to study the nature of the Ca(II) binding sites in the central cavity of the two-zinc(II) insulin hexamer. The dependence of the Eu(III) fluorescence lifetime upon Eu(III) stoichiometry indicates that there are three identical Eu(III) binding sites present in the two-zinc(II) insuli...

journal_title：Biochemistry

authors： Alameda GK,Evelhoch JL,Sudmeier JL,Birge RR

更新日期：1985-03-26 00:00:00

abstract：:Brief exposure of purple membrane (PM) to dilute Triton X-100 eliminates the actinic light effect on the relative amounts of fast M (Mf) and slow M (Ms) intermediates and alters the character and kinetics of the photocycle, without destroying the native BR trimers (Mukhopadhyay et al., 1994). Particular membrane lipid...

journal_title：Biochemistry

authors： Mukhopadhyay AK,Dracheva S,Bose S,Hendler RW

更新日期：1996-07-16 00:00:00

abstract：:Human NFU (also known as HIRIP5) has been implicated in cellular iron-sulfur cluster biosynthesis. Bacterial and yeast forms are smaller than the human protein and are homologous to the C-terminal domain of the latter. This C-terminal domain contains a pair of redox active cysteines and demonstrates thioredoxin-like a...

journal_title：Biochemistry

authors： Liu Y,Qi W,Cowan JA

更新日期：2009-02-10 00:00:00

abstract：:The explosion of protein sequence information requires that current strategies for function assignment evolve to complement experimental approaches with computationally based function prediction. This necessitates the development of strategies based on the identification of sequence markers in the form of specificity ...

journal_title：Biochemistry

authors： Huang H,Patskovsky Y,Toro R,Farelli JD,Pandya C,Almo SC,Allen KN,Dunaway-Mariano D

更新日期：2011-10-18 00:00:00

abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...

journal_title：Biochemistry

authors： Teissie J,Tsong TY

更新日期：1981-03-17 00:00:00

abstract：:The number of free cysteines in each polypeptide of acetylcholine receptor from the electric organ of Torpedo californica has been assessed by alkylating the native protein with N-ethylmaleimide and iodoacetamide during homogenization of the tissue and alkylating the polypeptides with N-ethylmaleimide as they were unf...

journal_title：Biochemistry

authors： Kellaris KV,Ware DK,Smith S,Kyte J

更新日期：1989-04-18 00:00:00

abstract：:Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...

journal_title：Biochemistry

authors： Haque ME,McIntosh TJ,Lentz BR

更新日期：2001-04-10 00:00:00

abstract：:The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...

journal_title：Biochemistry

authors： Lau FW,Bowie JU

更新日期：1997-05-13 00:00:00

abstract：:The inhibitory effects of urea on the normally rapid fibrillation of human calcitonin (hCT) were investigated by 1H NMR. From subtle differences in the chemical shift of hCT in the presence and absence of urea, the occurrence of weak interactions between urea and hCT was confirmed. The chemical shifts on the NH and C ...

journal_title：Biochemistry

authors： Kanaori K,Nosaka AY

更新日期：1996-10-01 00:00:00

abstract：:In the nacre or aragonite layer of the mollusk shell, proteomes that regulate both the early stages of nucleation and nano-to-mesoscale assembly of nacre tablets from mineral nanoparticle precursors exist. Several approaches have been developed to understand protein-associated mechanisms of nacre formation, yet we sti...

journal_title：Biochemistry

authors： Chang EP,Roncal-Herrero T,Morgan T,Dunn KE,Rao A,Kunitake JA,Lui S,Bilton M,Estroff LA,Kröger R,Johnson S,Cölfen H,Evans JS

更新日期：2016-04-26 00:00:00

abstract：:Doublet signal and singlet-like signal induced in Ca(2+)-depleted PS II were investigated by pulsed EPR in one-dimensionally oriented photosystem (PS) II membranes. The doublet signal showed marked angular dependent change in its spectrum in term of the applied magnetic field, indicating that the magnetic dipole-dipol...

journal_title：Biochemistry

authors： Mino H,Kawamori A,Ono T

更新日期：2000-09-12 00:00:00

abstract：:Dopamine (DA) is an unstable neurotransmitter that readily oxidizes to the DA quinone and forms reactive oxygen species, such as superoxide and hydrogen peroxide. The oxidized dopamine also forms thiol conjugates with sulfhydryl groups on cysteine, glutathione, and proteins. In the present study, we determined the red...

journal_title：Biochemistry

authors： Akagawa M,Ishii Y,Ishii T,Shibata T,Yotsu-Yamashita M,Suyama K,Uchida K

更新日期：2006-12-19 00:00:00

abstract：:Dynamic conformations of two distinct immunoglobulin (Ig) isotypes, murine IgE and human IgG1, were examined with fluorescence resonance energy transfer measurements. The IgE mutant epsilon/C gamma 3* and the IgG1 mutant gamma/C gamma 3* each bind [5-(dimethylamino)naphthalen-1-yl]sulfonyl (DNS) in two identical antig...

journal_title：Biochemistry

authors： Zheng Y,Shopes B,Holowka D,Baird B

更新日期：1992-08-25 00:00:00

abstract：:The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...

journal_title：Biochemistry

authors： Greenspoon N,Hershkoviz R,Alon R,Varon D,Shenkman B,Marx G,Federman S,Kapustina G,Lider O

更新日期：1993-02-02 00:00:00

abstract：:A stereochemical analysis has been carried out on two vinylpyruvate hydratases (VPH), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4S-hydroxypentanoate in meta-fission pathways. Bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. The analysis was carried out usin...

journal_title：Biochemistry

authors： Johnson WH Jr,Stack TM,Taylor SM,Burks EA,Whitman CP

更新日期：2016-07-26 00:00:00

abstract：:We have studied the hydrogen-exchange kinetics of hemoglobin A0 as a function of ligand, CO, saturation. In the noncooperative system, azide binding to methemoglobin, the alterations in exchange kinetics are proportional to the average degree of ligation. However, in the case of CO binding to deoxyhemoglobin the chang...

journal_title：Biochemistry

authors： Hallaway PE,Hallaway BE,Rosenberg A

更新日期：1984-01-17 00:00:00

abstract：:Enzymic catalysis proceeds via intermediates formed in the course of substrate conversion. Here, we directly detect key intermediates in thiamin diphosphate (ThDP)-dependent enzymes during catalysis using (1)H NMR spectroscopy. The quantitative analysis of the relative intermediate concentrations allows the determinat...

journal_title：Biochemistry

authors： Tittmann K,Golbik R,Uhlemann K,Khailova L,Schneider G,Patel M,Jordan F,Chipman DM,Duggleby RG,Hübner G

更新日期：2003-07-08 00:00:00

abstract：:A series of 24-homologated 1,25-dihydroxyvitamin D3 compounds have been chemically synthesized and studied with regard to their activity in inducing differentiation of human promyelocyte HL-60 cells to monocytes and in calcium mobilizing activity in vitamin D deficient rats. Homologation of 1,25-dihydroxyvitamin D3 or...

journal_title：Biochemistry

authors： Perlman K,Kutner A,Prahl J,Smith C,Inaba M,Schnoes HK,DeLuca HF

更新日期：1990-01-09 00:00:00

abstract：:N-Terminal glutaminyl and glutamyl residues of peptides and proteins tend to form pyroglutamic acid (pGlu) by intramolecular cylization. The rate constants for spontaneous cyclization of glutamine (10(-6) s(-1)) and glutamic acid (10(-9) s(-1)) in aqueous solution differ by approximately 3 orders of magnitude at pH 6....

journal_title：Biochemistry

authors： Seifert F,Schulz K,Koch B,Manhart S,Demuth HU,Schilling S

更新日期：2009-12-22 00:00:00

abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...

journal_title：Biochemistry

authors： Kawashima E,Gadek T,Caruthers MH

更新日期：1977-09-20 00:00:00