Purification and characterization of corn glutathione S-transferase.

abstract：:Transport of lactate, pyruvate, and the ketone bodies, acetoacetate and beta-hydroxybutyrate, is mediated in many mammalian cells by the monocarboxylate transporter MCT1. To be accepted as a substrate, a carboxyl group and an unpolar side chain are necessary. Site-directed mutagenesis of the rat MCT1 was used to ident...

journal_title：Biochemistry

authors： Rahman B,Schneider HP,Bröer A,Deitmer JW,Bröer S

更新日期：1999-08-31 00:00:00

abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...

journal_title：Biochemistry

authors： Echtay KS,Bienengraeber M,Klingenberg M

更新日期：1997-07-08 00:00:00

abstract：:A PS I membrane preparation from a PS II deficient mutant of Synechocystis sp. PCC 6803 (psb DI/DII/C) was investigated by picosecond photovoltage and fluorescence measurements. The photovoltage kinetics show two distinct phases. At low excitation energies the fast phase correlates with the fluorescence decay time con...

journal_title：Biochemistry

authors： Hecks B,Wulf K,Breton J,Leibl W,Trissl HW

更新日期：1994-07-26 00:00:00

abstract：:The primary ligand-binding protein (CNTFR alpha) of the multicomponent receptor for ciliary neurotrophic factor was produced in Escherichia coli. Using novel applications of size-exclusion chromatography and a protein gel-shift assay, we obtained quantitative separation of correctly refolded protein, as well as analyt...

journal_title：Biochemistry

authors： Panayotatos N,Everdeen D,Liten A,Somogyi R,Acheson A

更新日期：1994-05-17 00:00:00

abstract：:The oxygen enzymatically inserted as a hydroxyl function by chick kidney homogenate into the 24 position of 25-hydroxyvitamin D3 to give 24,25-dihydroxyvitamin D3 is derived exclusively from 18O2. Therefore, like the 25-hydroxyvitamin D3-lalpha-hydroxylase system, the 25-hydroxyvitamin D3-24-hydroxylase system is also...

journal_title：Biochemistry

authors： Madhok TC,Schnoes HK,DeLuca HF

更新日期：1977-05-17 00:00:00

abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...

journal_title：Biochemistry

authors： An S,Yang J,So SW,Zeng L,Goetzl EJ

更新日期：1994-12-06 00:00:00

abstract：:The effect of normo (NTG)- and hypertriglyceridemic (HTG)-VLDL on cultured human umbilical vein endothelial cell (HUVEC) surface-localized fibrinolysis was examined following pre-incubation with NTG-, HTG-VLDL, LDL (1-20 micrograms/mL) or buffer (control). Ligand binding assays, using 125I-labeled tcu-PA, t-PA, or Glu...

journal_title：Biochemistry

authors： Li XN,Koons JC,Benza RL,Parks JM,Varma VK,Bradley WA,Gianturco SH,Taylor KB,Grammer JR,Tabengwa EM,Booyse FM

更新日期：1996-05-14 00:00:00

abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator (tPA), but reacts with thrombin at a much slower rate. Based on the previous mutagenesis studies and the X-ray crystal structure of the thrombin E192Q-bovine pancrea...

journal_title：Biochemistry

authors： Rezaie AR

更新日期：1998-09-22 00:00:00

abstract：:We have characterized the importance of size, sulfation, and anticoagulant activity of heparin in release of basic fibroblast growth factor (bFGF) from the subendothelial extracellular matrix (ECM) and the luminal surface of the vascular endothelium. For this purpose, 125I-bFGF was first incubated with ECM and conflue...

journal_title：Biochemistry

authors： Ishai-Michaeli R,Svahn CM,Weber M,Chajek-Shaul T,Korner G,Ekre HP,Vlodavsky I

更新日期：1992-02-25 00:00:00

abstract：:Tafazzin is the mitochondrial enzyme that catalyzes transacylation between a phospholipid and a lysophospholipid in remodeling. Mutations in tafazzin cause Barth syndrome, a potentially life-threatening disease with the major symptom being cardiomyopathy. In the tafazzin-deficient heart, cardiolipin (CL) acyl chains b...

journal_title：Biochemistry

authors： Kimura T,Kimura AK,Ren M,Berno B,Xu Y,Schlame M,Epand RM

更新日期：2018-04-10 00:00:00

abstract：:Glutamate semialdehyde aminomutase is a recognized target for selective herbicides and antibacterial agents because it provides the aminolevulinate from which tetrapyrroles are synthesized in plants and bacteria but not in animals. The reactions of the enzyme with R- and S-enantiomers of a novel compound, diaminopropy...

journal_title：Biochemistry

authors： Contestabile R,Jenn T,Akhtar M,Gani D,John RA

更新日期：2000-03-21 00:00:00

abstract：:The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG) is responsible for the removal of uracil in DNA as the first step in the base excision repair (BER) pathway. AFUDG contains a large solvent-exposed peptide region containing an α helix and loop anchored on each end v...

journal_title：Biochemistry

authors： Engstrom LM,Partington OA,David SS

更新日期：2012-06-26 00:00:00

abstract：:Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococc...

journal_title：Biochemistry

authors： Majsnerowska M,Ter Beek J,Stanek WK,Duurkens RH,Slotboom DJ

更新日期：2015-08-11 00:00:00

abstract：:The structure of HIV protease (HIV Pr) bound to JE-2147 (also named AG1776 or KNI-764) is determined here to 1.09 A resolution. This highest-resolution structure for HIV Pr allows refinement of anisotropic displacement parameters (ADPs) for all atoms. Clustering based on the directional information in ADPs defines two...

journal_title：Biochemistry

authors： Reiling KK,Endres NF,Dauber DS,Craik CS,Stroud RM

更新日期：2002-04-09 00:00:00

abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...

journal_title：Biochemistry

authors： Han H,Yang Y,Olesen SH,Becker A,Betzi S,Schönbrunn E

更新日期：2010-05-18 00:00:00

abstract：:Calcium and its receptor protein calmodulin function in the regulation of proliferation of mammalian cells. A 68 kDa calmodulin-specific binding protein was shown previously to be associated with growth factor-dependent progression of a variety of mammalian cells from G1 to S phase and to stimulate DNA synthesis in pe...

journal_title：Biochemistry

authors： Cao QP,McGrath CA,Baril EF,Quesenberry PJ,Reddy GP

更新日期：1995-03-28 00:00:00

abstract：:The structure of human epidermal growth factor (EGF, 53 amino acids) comprises three distinct loops (A, B, and C) connected correspondingly by the three native disulfide bonds, Cys(6)-Cys(20), Cys(14)-Cys(31), and Cys(33)-Cys(42). The connection of Cys(6) and Cys(20) forming the N-terminal A loop is essential for the ...

journal_title：Biochemistry

authors： Lu BY,Jiang C,Chang JY

更新日期：2005-11-15 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:Steroid-receptor complexes formed at low temperature and ionic strength are unable to bind to target cell nuclei. After a temporary exposure to high ionic strength and/or temperature they become activated (i.e., able to bind to nuclei). However, there exists an equilibrium between activated and nonactivated complexes;...

journal_title：Biochemistry

authors： Atger M,Milgrom E

更新日期：1976-09-21 00:00:00

abstract：:Antithrombin III (ATIII) is a plasma-borne serine protease inhibitor that apparently forms covalent complexes with thrombin. The interaction between ATIII and thrombin is enhanced several thousandfold by the glycosaminoglycan, heparin. We have previously proposed that the heparin binding site of ATIII resides within a...

journal_title：Biochemistry

authors： Smith JW,Dey N,Knauer DJ

更新日期：1990-09-25 00:00:00

abstract：:Plasmids containing a site-specifically incorporated O6-methyl- (m6G), O6-ethyl- (e6G), or O6-benzylguanine (b6G) within the ATG initiation codon of the lacZ' gene were used to transform Escherichia coli that were repair proficient or deficient in one or both of the E. coli O6-alkylguanine-DNA alkyltransferases, the u...

journal_title：Biochemistry

authors： Pauly GT,Hughes SH,Moschel RC

更新日期：1994-08-09 00:00:00

abstract：:The binding of ethidium bromide, as monitored by fluorescence enhancement, to chromatin prepared by nuclease digestion has been compared with the binding of the dye to sheared chromatin. The nuclease preparation (native chromatin) is characterized by a high affinity region of the Scatchard plot (r = 0-0.025, K1 = 1 X ...

journal_title：Biochemistry

authors： Paoletti J,Magee BB,Magee PT

更新日期：1977-02-08 00:00:00

abstract：:Periodate-oxidized ATP (o-ATP) was prepared as an affinity label of nucleotide binding sites on the chloroform-released ox heart mitochondrial ATPase. In the presence of MgSO4, o-ATP is a substrate for the ATPase. It can act as a reversible, competitive inhibitor of ATPase activity and can also induce an irreversible ...

journal_title：Biochemistry

authors： Lowe PN,Beechey RB

更新日期：1982-08-17 00:00:00

abstract：:Fluorescence studies of the binding of IKe gene 5 protein to various polynucleotides were performed to obtain insight into the question as to what extent the binding characteristics of the gene 5 proteins of the IKe and M13 phages resemble and/or differ from each other. The fluorescence of IKe gene 5 protein is quench...

journal_title：Biochemistry

authors： de Jong EA,Harmsen BJ,Konings RN,Hilbers CW

更新日期：1987-04-07 00:00:00

abstract：:The reaction of cytochrome c oxidase with hydrogen peroxide has been of great value in generating and characterizing oxygenated species of the enzyme that are identical or similar to those formed during turnover of the enzyme with dioxygen. Most previous studies have utilized relatively low peroxide concentrations (mi...

journal_title：Biochemistry

authors： Zaslavsky D,Smirnova IA,Brzezinski P,Shinzawa-Itoh K,Yoshikawa S,Gennis RB

更新日期：1999-11-30 00:00:00

abstract：:Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...

journal_title：Biochemistry

authors： Rajapaksa R,Abu-Soud H,Raushel FM,Harris BG,Cook PF

更新日期：1993-03-02 00:00:00

abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...

journal_title：Biochemistry

authors： Chen B,Mayer MU,Squier TC

更新日期：2005-03-29 00:00:00

abstract：:The endogenous phosphoserine residue in porcine pepsinogen has been titrated with use of phosphorus-31 nuclear magnetic resonance (31P NMR). It has an observed pKa2 of 6.7 and a narrow line width (congruent to 10 Hz). The phosphate can be readily removed by an acid phosphatase from potato; however, it is resistant to ...

journal_title：Biochemistry

authors： Williams SP,Bridger WA,James MN

更新日期：1986-10-21 00:00:00

abstract：:Solid-state NMR spectroscopy was employed to study the molecular dynamics of the colicin Ia channel domain in the soluble and membrane-bound states. In the soluble state, the protein executes small-amplitude librations (with root-mean-square angular fluctuations of 0-10 degrees ) in the backbone and larger-amplitude m...

journal_title：Biochemistry

authors： Huster D,Xiao L,Hong M

更新日期：2001-06-26 00:00:00

abstract：:Peptide mapping, chemical sequencing, microbore HPLC/electrospray ionization mass spectrometry (LC/ESI/MS), and matrix-assisted laser desorption mass spectrometry (MALDI/MS) were used to identify the sites of intra- and intermolecular disulfide linkages in bovine dopamine beta-hydroxylase. The enzyme contains 14 cyste...

journal_title：Biochemistry

authors： Robertson JG,Adams GW,Medzihradszky KF,Burlingame AL,Villafranca JJ

更新日期：1994-09-27 00:00:00