Identification of the sites phosphorylated by cyclic AMP-dependent protein kinase on the beta 2 subunit of L-type voltage-dependent calcium channels.

abstract：:Aristolochene synthase, a metal-dependent sesquiterpene cyclase from Aspergillus terreus, catalyzes the ionization-dependent cyclization of farnesyl diphosphate (FPP) to form the bicyclic eremophilane (+)-aristolochene with perfect structural and stereochemical precision. Here, we report the X-ray crystal structure of...

journal_title：Biochemistry

authors： Chen M,Al-lami N,Janvier M,D'Antonio EL,Faraldos JA,Cane DE,Allemann RK,Christianson DW

更新日期：2013-08-13 00:00:00

abstract：:The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosX(Ml)) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activi...

journal_title：Biochemistry

authors： Brown DW,Schaab MR,Birmingham WR,Armstrong RN

更新日期：2009-03-10 00:00:00

abstract：:The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...

journal_title：Biochemistry

authors： Seela F,Tran TQ,Mentzel H,Erdmann VA

更新日期：1981-04-28 00:00:00

abstract：:We report cloning, expression in Escherichia coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the highest affini...

journal_title：Biochemistry

authors： Sineva EV,Davydov DR

更新日期：2010-12-21 00:00:00

abstract：:Analogs of EPSP (4-8) have been prepared, and their activity as substrates for the chorismate synthase from Neurospora crassa has been characterized kinetically. The enzyme appears to show strict discrimination against substitution at the Z-position of the enol ether side chain as well as against substitution at the S...

journal_title：Biochemistry

authors： Lauhon CT,Bartlett PA

更新日期：1994-11-29 00:00:00

abstract：:Members of the ATP-binding cassette (ABC) transporters share the same basic architecture, with a four-core domain made of two transmembrane plus two nucleotide-binding domains. However, a supramolecular organization has been detected in some ABC transporters, which might be relevant to physiological regulation of subs...

journal_title：Biochemistry

authors： Dalmas O,Do Cao MA,Lugo MR,Sharom FJ,Di Pietro A,Jault JM

更新日期：2005-03-22 00:00:00

abstract：:The reduction potentials of the compound II/ferric and compound I/compound II couples have been studied, using potassium hexachloroiridate as a mediator titrant, by thin-layer spectroelectrochemistry. Compound I, which is 2 equiv more oxidized than the ferric (i.e., resting) form of the enzyme, was reversibly formed v...

journal_title：Biochemistry

authors： Farhangrazi ZS,Fossett ME,Powers LS,Ellis WR Jr

更新日期：1995-03-07 00:00:00

abstract：:Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...

journal_title：Biochemistry

authors： Watson J,Nilsen-Hamilton M,Hamilton RT

更新日期：1976-04-06 00:00:00

abstract：:Beta-lactamases are the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and cephalosporins. Transition-state analogues that bind to the enzymes with nanomolar affinities have been introduced in an effort to reverse the resistance conferred by these enzymes. To understand the or...

journal_title：Biochemistry

authors： Roth TA,Minasov G,Morandi S,Prati F,Shoichet BK

更新日期：2003-12-16 00:00:00

abstract：:Granulocyte-colony stimulating factor (G-CSF) binds to a specific cell surface receptor and induces signals for growth and differentiation in cells of granulocyte hematopoietic lineage. In order to understand how G-CSF binding initiates signals into these cells, we have studied its interactions with the entire extrace...

journal_title：Biochemistry

authors： Horan T,Wen J,Narhi L,Parker V,Garcia A,Arakawa T,Philo J

更新日期：1996-04-16 00:00:00

abstract：:The precise nature of redox modulation of N-methyl-d-aspartate (NMDA) receptors is still unclear, although it is thought to be related to the formation and breaking of disulfide bonds. Recent structural data demonstrated the way in which disulfide bonds in the ligand-binding core of the NR1 subunit are arranged. Howev...

journal_title：Biochemistry

authors： Kaye SL,Sansom MS,Biggin PC

更新日期：2007-02-27 00:00:00

abstract：:The O-linked protein glycosylation pathway in Neisseria gonorrhoeae is responsible for the synthesis of a complex oligosaccharide on undecaprenyl diphosphate and subsequent en bloc transfer of the glycan to serine residues of select periplasmic proteins. Protein glycosylation (pgl) genes have been annotated on the bas...

journal_title：Biochemistry

authors： Hartley MD,Morrison MJ,Aas FE,Børud B,Koomey M,Imperiali B

更新日期：2011-06-07 00:00:00

abstract：:The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidity of the active site zinc ion. ...

journal_title：Biochemistry

authors： Mock WL,Tsay JT

更新日期：1986-05-20 00:00:00

abstract：:The X-ray crystallographic structure of the photosynthetic reaction center from Rhodobacter sphaeroides obtained at high resolution has revealed a number of internal water molecules (Ermler, U., Fritzsch, G., Buchanan, S. K., and Michel, H. (1994) Structure 2, 925-936; Stowell, M. H. B., McPhillips, T. M., Rees, D. C....

journal_title：Biochemistry

authors： Tandori J,Sebban P,Michel H,Baciou L

更新日期：1999-10-05 00:00:00

abstract：:Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes the transfer of the amino group of L-histidinol phosphate (Hsp) to 2-oxoglutarate to form imidazole acetol phosphate (IAP) and glutamate. Thus, HspAT recognizes two kinds of substrates, Hsp and glutamat...

journal_title：Biochemistry

authors： Haruyama K,Nakai T,Miyahara I,Hirotsu K,Mizuguchi H,Hayashi H,Kagamiyama H

更新日期：2001-04-17 00:00:00

abstract：:Apolipoprotein E (apoE)-knockout mice develop severe atherosclerosis associated with high levels of very low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) in plasma. To investigate the atherogenic role of VLDL and IDL, the lipoprotein fraction containing both VLDL and IDL (apoEko-VLDL/IDL) was ...

journal_title：Biochemistry

authors： Hakamata H,Sakaguchi H,Zhang C,Sakashita N,Suzuki H,Miyazaki A,Takeya M,Takahashi K,Kitamura N,Horiuchi S

更新日期：1998-09-29 00:00:00

abstract：:Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...

journal_title：Biochemistry

authors： Luria A,Vegelyte-Avery V,Stith B,Tsvetkova NM,Wolkers WF,Crowe JH,Tablin F,Nuccitelli R

更新日期：2002-11-05 00:00:00

abstract：:We have labeled the primer binding domain of murine leukemia virus reverse transcriptase (MuLV RT) by covalently cross-linking 5' end labeled d(T)8 to MuLV RT, using ultraviolet light energy. The specificity and the functional significance of the primer cross-linking reaction were demonstrated by the fact that (i) oth...

journal_title：Biochemistry

authors： Tirumalai RS,Modak MJ

更新日期：1991-07-02 00:00:00

abstract：:The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...

journal_title：Biochemistry

authors： Rickert KW,Sears J,Beck WF,Brudvig GW

更新日期：1991-08-13 00:00:00

abstract：:The effects of nearest neighbor interactions between a nucleotide base at the primer 3'-terminus and an incoming deoxyribonucleoside triphosphate on DNA polymerase catalyzed insertion were examined. Kinetics of inserting the fluorescent nucleotide analog 2-aminopurine deoxyribonucleotide (dAPMP) and dAMP opposite a te...

journal_title：Biochemistry

authors： Bloom LB,Otto MR,Beechem JM,Goodman MF

更新日期：1993-10-19 00:00:00

abstract：:Database searches indicated that the genome of Bacillus subtilis contains three different genes encoding RNase H homologues. The ypdQ gene encodes an RNase HI homologue with 132 amino acid residues, whereas the rnh and ysgB genes encode RNase HII homologues with 255 and 313 amino acid residues, respectively. RNases HI...

journal_title：Biochemistry

authors： Ohtani N,Haruki M,Morikawa M,Crouch RJ,Itaya M,Kanaya S

更新日期：1999-01-12 00:00:00

abstract：:The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...

journal_title：Biochemistry

authors： Fryklund L,Eaker D

更新日期：1975-07-01 00:00:00

abstract：:Despite a number of studies, the formation of the Michaelis complexes between ferredoxin-NADP (+) reductases and NADP(H) eluded detailed investigations by rapid kinetic techniques because of their high formation rates. Moreover, the reversible nature of the reaction of hydride ion transfer between these enzymes and NA...

journal_title：Biochemistry

authors： Pennati A,Zanetti G,Aliverti A,Gadda G

更新日期：2008-03-18 00:00:00

abstract：:The catalytically competent active-site structure of a true acylenzyme reaction intermediate of TEM-1 beta-lactamase formed with the kinetically specific spin-labeled substrate 6-N-(2,2,5,5-tetramethyl-1-oxypyrrolinyl-3-carboxyl)-penicillanic acid isolated under cryoenzymologic conditions has been determined by angle-...

journal_title：Biochemistry

authors： Mustafi D,Sosa-Peinado A,Makinen MW

更新日期：2001-02-27 00:00:00

abstract：:Acholeplasma laidlawii B cells, highly enriched in saturated C14 and C15 fatty acids, have been grown at several temperatures. Conformational order in the acyl chains of live cell membranes has been monitored with Fourier-transform infrared (FT-IR) spectroscopy. Coupled CH2 wagging progressions, characteristic of the ...

journal_title：Biochemistry

authors： Moore DJ,Mendelsohn R

更新日期：1994-04-05 00:00:00

abstract：:Four different temperature-sensitive M protein mutants (tsM) of vesicular stomatitis virus (VSV) were characterized with regard to the association of the mutated M protein either with nucleocapsids or with membranes in the intact virions. Virions were labeled with the photoreactive hydrophobic probe [125I]iodonaphthyl...

journal_title：Biochemistry

authors： Mancarella DA,Lenard J

更新日期：1981-11-24 00:00:00

abstract：:We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...

journal_title：Biochemistry

authors： Sidik K,Smerdon MJ

更新日期：1990-08-14 00:00:00

abstract：:Apamin is being studied as a model for the folding mechanism of proteins whose structures are stabilized by disulfide bonds. Apamin consists of 18 amino acid residues and forms a stable structure consisting of a C-terminal alpha-helix and two reverse turns. This structure is stabilized by two disulfide bonds connectin...

journal_title：Biochemistry

authors： Chau MH,Nelson JW

更新日期：1992-05-12 00:00:00

abstract：:We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...

journal_title：Biochemistry

authors： Godson GN,Mustaev AA,Sun W

更新日期：1998-03-17 00:00:00

abstract：:N-linked, high-mannose-type oligosaccharides lacking glucose residues may be transiently glucosylated directly from UDP-Glc in the endoplasmic reticulum of mammalian, plant, fungal, and protozoan cells. The products formed have been identified as N-linked Glc1Man5-9GlcNAc2 and glucosidase II is apparently the enzyme r...

journal_title：Biochemistry

authors： Gañán S,Cazzulo JJ,Parodi AJ

更新日期：1991-03-26 00:00:00