1H nuclear magnetic resonance double resonance study of oxytocin in aqueous solution.

abstract：:The question of the possible identity of catalytic and regulatory proton pathways in the chloroplast FoF1 ATPase has been studied using different energy-transfer inhibitors. Venturicidin, a reversible inhibitor of Fo, affects neither the delta mu H(+)-dependent thiol reduction of the membrane-bound chloroplast ATPase ...

journal_title：Biochemistry

authors： Valerio M,de Kouchkovsky Y,Haraux F

更新日期：1992-05-05 00:00:00

abstract：:The reactivity of aluminum and several other divalent and trivalent metallic cations toward chromatin from rat brain and liver has been investigated. Two criteria are used to determine the relative reactivity of these cations toward chromatin. The first involves the ability of the ions to compact the chromatin fibers ...

journal_title：Biochemistry

authors： Walker PR,LeBlanc J,Sikorska M

更新日期：1989-05-02 00:00:00

abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...

journal_title：Biochemistry

authors： Woodyer R,van der Donk WA,Zhao H

更新日期：2003-10-14 00:00:00

abstract：:Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...

journal_title：Biochemistry

authors： Ohanian V,Wolfe LC,John KM,Pinder JC,Lux SE,Gratzer WB

更新日期：1984-09-11 00:00:00

abstract：:Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dim...

journal_title：Biochemistry

authors： Pocanschi CL,Dahmane T,Gohon Y,Rappaport F,Apell HJ,Kleinschmidt JH,Popot JL

更新日期：2006-11-28 00:00:00

abstract：:Understanding the structural basis for defects in protein function that underlie protein-based genetic diseases is the fundamental requirement for development of therapies. This situation is epitomized by the cystic fibrosis transmembrane conductance regulator (CFTR)-the gene product known to be defective in CF patien...

journal_title：Biochemistry

authors： Cheung JC,Deber CM

更新日期：2008-02-12 00:00:00

abstract：:Hemoglobins extracted from fishes that live in temperate waters show little or no dissociation even in the liganded form, unlike human hemoglobin (HbA). To establish whether cold adaptation influences the tendency to dissociate, the dimer-tetramer association constants (L(2,4)) of the carbonmonoxy derivatives of repre...

journal_title：Biochemistry

authors： Giangiacomo L,D'Avino R,di Prisco G,Chiancone E

更新日期：2001-03-13 00:00:00

abstract：:The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...

journal_title：Biochemistry

authors： Zimmermann JL,Amano T,Sigalat C

更新日期：1999-11-16 00:00:00

abstract：:Inheritance of apoE4 is a strong risk factor for the development of late-onset sporadic Alzheimer's disease (AD). Several lines of evidence suggest that apoE4 binds to the Alzheimer Abeta protein and, under certain experimental conditions, promotes formation of beta-sheet structures and amyloid fibrils. Deposition of ...

journal_title：Biochemistry

authors： Poeggeler B,Miravalle L,Zagorski MG,Wisniewski T,Chyan YJ,Zhang Y,Shao H,Bryant-Thomas T,Vidal R,Frangione B,Ghiso J,Pappolla MA

更新日期：2001-12-11 00:00:00

abstract：:Epitranscriptomic RNA modifications can serve as recognition elements for the recruitment of effector proteins (i.e., "readers") to modified transcripts. While these interactions play an important role in mRNA regulation, there is a major gap in our understanding of the sequence determinants critical for the binding o...

journal_title：Biochemistry

authors： Arguello AE,Leach RW,Kleiner RE

更新日期：2019-08-06 00:00:00

abstract：:Sedimentation experiments with closed circular duplex PM2 deoxyribonucleic acid (DNA) and viscosity measurements with sonicated rodlike DNA fragments have been performed to investigate unwinding and extension of the DNA helix associated with binding of homologous series of diacridines. When the acridine nuclei are lin...

journal_title：Biochemistry

authors： Wright RG,Wakelin LP,Fieldes A,Acheson RM,Waring MJ

更新日期：1980-12-09 00:00:00

abstract：:In a previous report, the cDNA for human proteinase inhibitor 8 (PI8) was first identified, isolated, and subcloned into a mammalian expression vector and expressed in baby hamster kidney cells. Initial studies indicated that PI8 was able to inhibit the amidolytic activity of trypsin and form an SDS-stable approximate...

journal_title：Biochemistry

authors： Dahlen JR,Foster DC,Kisiel W

更新日期：1997-12-02 00:00:00

abstract：:Aminopeptidase B (APB, EC 3.4.11.6) preferentially hydrolyzes the N-terminal basic amino acids of synthetic and peptide substrates and requires a physiological concentration of NaCl for optimal activity. In this study, we used site-directed mutagenesis and molecular modeling to search for an amino acid residue that is...

journal_title：Biochemistry

authors： Ohnishi A,Watanabe J,Ogawa Y,Goto Y,Hattori A,Tsujimoto M

更新日期：2015-10-06 00:00:00

abstract：:Pluronic block copolymers (triblock copolymers of poly(ethylene oxide) and poly(propylene oxide)) exhibit a chemosensitizing effect on multidrug resistant cell lines. Changes in membrane permeability are hypothesized to be responsible because inhibition of drug transport mediated by both the multidrug-resistance-assoc...

journal_title：Biochemistry

authors： Krylova OO,Pohl P

更新日期：2004-03-30 00:00:00

abstract：:A procedure was developed for selective radiolabeling of membrane proteins on cells to higher specific activities than possible with available techniques. Cell surface amino groups were derivatized with 125I-(hydroxyphenyl)propionyl groups via 125I-sulfosuccinimidyl (hydroxyphenyl)propionate (125I-sulfo-SHPP). This re...

journal_title：Biochemistry

authors： Thompson JA,Lau AL,Cunningham DD

更新日期：1987-02-10 00:00:00

abstract：:The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...

journal_title：Biochemistry

authors： Caspary WJ,Lanzo DA,Niziak C

更新日期：1982-01-19 00:00:00

abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

journal_title：Biochemistry

authors： Earle SR,O'Neal SG,Fisher RR

更新日期：1978-10-31 00:00:00

abstract：:The constant fragment of the immunoglobulin light chain in which the intramolecular disulfide bond is reduced (reduced CL fragment) assumes a conformation very similar to that of the intact CL fragment and contains two sulfhydryl groups buried in the interior of the molecule [Goto, Y., & Hamaguchi, K. (1979) J. Bioche...

journal_title：Biochemistry

authors： Goto Y,Hamaguchi K

更新日期：1986-05-20 00:00:00

abstract：:The C2 domain is a Ca2+-dependent, membrane-targeting motif originally discovered in protein kinase C and recently identified in numerous eukaryotic signal-transducing proteins, including cytosolic phospholipase A2 (cPLA2) of the vertebrate inflammation pathway. Intracellular Ca2+ signals recruit the C2 domain of cPLA...

journal_title：Biochemistry

authors： Nalefski EA,Slazas MM,Falke JJ

更新日期：1997-10-07 00:00:00

abstract：:The interaction of single chain urokinase with its receptor accelerates plasminogen activator activity on cell surfaces and induces intracellular signalling in several cell types. To date, no physiologic inhibitor of this binding has been identified. We report that the binding of scuPA to its cellular receptor is inhi...

journal_title：Biochemistry

authors： al-Roof Higazi A,Aceto JF,Kniss D,Upson R,Cohen R,Dichek DA,Cines DB

更新日期：1996-05-28 00:00:00

abstract：:The purified PBS2 phage-coded inhibitor of uracil-DNA glycosylase (Ura-DNA glycosylase) from Bacillus subtilis has been tested for its ability to inhibit this enzyme isolated from other prokaryotic and from eukaryotic sources. In addition, the inhibitor has been assayed for its effect on DNA glycosylases specific for ...

journal_title：Biochemistry

authors： Karran P,Cone R,Friedberg EC

更新日期：1981-10-13 00:00:00

abstract：:The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...

journal_title：Biochemistry

authors： Liu J,Shu W,Fagan MB,Nunberg JH,Lu M

更新日期：2001-03-06 00:00:00

abstract：:In order to study in detail the generality of the NUX (N = A, U, G, or C; X = A, U, or C) rule for the GUC triplet adjacent to the cleavage site in hammerhead ribozymes, two kinetic parameters, namely, kcat and Km, were determined for substrates with mutations in this triplet, which included double mutants with mutati...

journal_title：Biochemistry

authors： Shimayama T,Nishikawa S,Taira K

更新日期：1995-03-21 00:00:00

abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...

journal_title：Biochemistry

authors： Wu HY,Behe MJ

更新日期：1985-09-24 00:00:00

abstract：:Annexin V is a Ca(2+)-dependent, phospholipid-binding protein that may have one or more membrane-related functions. The binding of annexin V to phospholipids in a detergent micelle matrix was studied to attempt to determine directly the stoichiometry of specific phospholipid-binding sites and the importance of negativ...

journal_title：Biochemistry

authors： Meers P,Mealy T

更新日期：1993-11-02 00:00:00

abstract：:The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled nativelike functional arrays of an aspartate receptor cytoplasmic fragment (CF) with its two cytoplasmic protein partners (CheA and Ch...

journal_title：Biochemistry

authors： Harris MJ,Struppe JO,Wylie BJ,McDermott AE,Thompson LK

更新日期：2016-07-05 00:00:00

abstract：:Tat, an essential human immunodeficiency virus type 1 protein interacts with the transactivation response element (TAR) and stimulates transcription from the viral long-terminal repeat (LTR). Blockage of Tat-TAR interaction halts viral transcription and hence replication. We have found that polyamide nucleic acid (PNA...

journal_title：Biochemistry

authors： Mayhood T,Kaushik N,Pandey PK,Kashanchi F,Deng L,Pandey VN

更新日期：2000-09-26 00:00:00

abstract：:Diverse voltage-gated K+ channels are produced by tetramerization of channel alpha subunits that can be encoded by multiple genes. In GH3 pituitary cells, the Kv1.4 and Kv1.5 subunit genes are constitutively expressed, and dexamethasone selectively up-regulates expression of the latter gene. Here we use subunit-specif...

journal_title：Biochemistry

authors： Takimoto K,Levitan ES

更新日期：1996-11-12 00:00:00

abstract：:Human acidic mammalian chitinase (AMCase), a member of the family 18 glycosyl hydrolases, is one of the important proteins involved in Th2-mediated inflammation and has been implicated in asthma and allergic diseases. Inhibition of AMCase results in decreased airway inflammation and airway hyper-responsiveness in a mo...

journal_title：Biochemistry

authors： Chou YT,Yao S,Czerwinski R,Fleming M,Krykbaev R,Xuan D,Zhou H,Brooks J,Fitz L,Strand J,Presman E,Lin L,Aulabaugh A,Huang X

更新日期：2006-04-11 00:00:00

abstract：:The effects of two oppositely charged pyrene derivatives, 1-pyrenebutyrylcholine (PBC) and 1-pyrenebutyric acid (PBA), on red blood cell shape have been examined. Both compounds convert normal biconcave erythrocytes into echinocytes. However, with extended incubation time at elevated temperature, the morphology of PBC...

journal_title：Biochemistry

authors： Matayoshi ED

更新日期：1980-07-22 00:00:00