Abstract：
:A procedure was developed for selective radiolabeling of membrane proteins on cells to higher specific activities than possible with available techniques. Cell surface amino groups were derivatized with 125I-(hydroxyphenyl)propionyl groups via 125I-sulfosuccinimidyl (hydroxyphenyl)propionate (125I-sulfo-SHPP). This reagent preferentially labeled membrane proteins exposed at the cell surface of erythrocytes as assessed by the degree of radiolabel incorporation into erythrocyte ghost proteins and hemoglobin. Comparison with the lactoperoxidase-[125I]iodide labeling technique revealed that 125I-sulfo-SHPP labeled cell surface proteins to a much higher specific activity and hemoglobin to a much lower specific activity. Additionally, this reagent was used for selective radiolabeling of membrane proteins on the cytoplasmic face of the plasma membrane by blocking exofacial amino groups with uniodinated sulfo-SHPP, lysing the cells, and then incubating them with 125I-sulfo-SHPP. Exclusive labeling of either side of the plasma membrane was demonstrated by the labeling of some marker proteins with well-defined spatial orientations on erythrocytes. Transmembrane proteins such as the epidermal growth factor receptor on cultured cells could also be labeled differentially from either side of the plasma membrane.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Thompson JA,Lau AL,Cunningham DDdoi
10.1021/bi00377a014subject
Has Abstractpub_date
1987-02-10 00:00:00pages
743-50issue
3eissn
0006-2960issn
1520-4995journal_volume
26pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034394t
更新日期：2003-06-24 00:00:00
abstract：:NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9920230
更新日期：2000-02-08 00:00:00
abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500854p
更新日期：2014-09-09 00:00:00
abstract：:We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00427a037
更新日期：1989-01-10 00:00:00
abstract：:Due to their prevalence and roles in biological systems, single mismatches adjacent to G-U pairs are important RNA structural elements. Since there are only limited experimental values for the stability of single mismatches adjacent to G-U pairs, current algorithms using free energy minimization to predict RNA seconda...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800471z
更新日期：2008-09-23 00:00:00
abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible cleavage of serine to form glycine and single carbon groups that are essential for many biosynthetic pathways. SHMT requires both pyridoxal phosphate (PLP) and tetrahydropteroylpolyglutamate (H4PteGlun) as cofactors, the latter as a carrier of the single ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9904151
更新日期：1999-06-29 00:00:00
abstract：:Arylamine N-acetyltransferases (NATs) catalyze an acetyl group transfer from acetyl coenzyme A (AcCoA) to arylamines, hydrazines, and their N-hydroxylated arylamine metabolites. The recently determined three-dimensional structures of prokaryotic NATs have revealed a cysteine protease-like Cys-His-Asp catalytic triad, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0497244
更新日期：2004-06-29 00:00:00
abstract：:Members of the phospholipase C-beta (PLC-beta) family of proteins are activated either by G alpha or G beta gamma subunits of heterotrimeric G proteins. To define specific regions of PLC-beta 3 that are involved in binding and activation by G beta gamma, a series of fragments of PLC-beta 3 as glutathione-S-transferase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992021f
更新日期：2000-02-22 00:00:00
abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00401a051
更新日期：1988-01-12 00:00:00
abstract：:An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0157159
更新日期：2001-11-13 00:00:00
abstract：:Solvent reorganization can contribute significantly to the energetics of protein-protein interactions. However, our knowledge of the magnitude of the energetic contribution is limited, in part, by a dearth of quantitative experimental measurements. The biotin repressor forms a homodimer as a prerequisite to DNA bindin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400952m
更新日期：2013-09-24 00:00:00
abstract：:Most proteins are denatured by heat treatment, and the process is usually irreversible. However, some proteins, such as hyperthermophilic proteins are known to be stable even at the boiling temperature of water. We here describe a systematic investigation of thermal behavior of proteins by purifying and characterizing...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001441y
更新日期：2000-12-05 00:00:00
abstract：:Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an imp...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi301441e
更新日期：2012-12-18 00:00:00
abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00615a011
更新日期：1978-10-31 00:00:00
abstract：:The intracellular kinetic folding pathway of the human chorionic gonadotropin beta-subunit (hCG-beta) reveals the presence of a disulfide between Cys residues 38-57 that is not detected by X-ray analysis of secreted hCG-beta. This led us to propose that disulfide rearrangement is an essential feature of cystine knot f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049856x
更新日期：2004-05-04 00:00:00
abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00172a042
更新日期：1994-02-15 00:00:00
abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002938a
更新日期：2001-08-07 00:00:00
abstract：:The annexin fold consists of four 70-residue domains with markedly homologous sequences and nearly identical structures. Each domain contains five helices designated A to E. Domain 2 of annexin I was obtained by chemical synthesis including ten specifically labeled residues and studied by 1H-15N NMR and circular dichr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960747v
更新日期：1996-08-13 00:00:00
abstract：:The fluorescence properties of three variants of alpha-lactalbumin (alpha-LA) containing a single tryptophan residue were investigated under native, molten globule, and unfolded conditions. These proteins have levels of secondary structure and stability similar to those of the wild type. The fluorescence signal in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010004w
更新日期：2001-06-19 00:00:00
abstract：:A new actinoleukin-like antitumor antibiotic, BBM-928A, has been shown to interact with isolated DNA molecules. BBM-928A contains two substituted quinolines linked by a cyclic decapeptide. Quenching effects of the covalently closed superhelical PM2 DNA on the BBM-928A fluorescence revealed a strong interaction with an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00565a012
更新日期：1980-11-25 00:00:00
abstract：:Insulin and insulin-like growth factor 1 (IGF-1) share high sequence homology, but their folding behaviors are significantly different: insulin folds into one unique thermodynamically controlled structure, while IGF-1 folds into two thermodynamically controlled disulfide isomers. However, the origin of their different...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0346289
更新日期：2003-08-19 00:00:00
abstract：:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00462a002
更新日期：1990-03-13 00:00:00
abstract：:The downfield (9-15 ppm) proton spectrum of Escherichia coli 5S RNA has been examined at 500 MHz by using nuclear Overhauser methods. The data confirm the existence of the terminal and procaryotic loop helices within the molecule [Fox, G. E., & Woese, C. R. (1975) Nature (London) 256, 505-506]. Very little stable, dou...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00280a005
更新日期：1983-05-24 00:00:00
abstract：:The effects of several guanine nucleotide analogues on (Na+,K+)-ATPase activity of membranes isolated from several tissues were analyzed to determine if a G-protein might be involved in the hormonal regulation of the (Na+,K+)-ATPase. Submillimolar concentrations of GTP gamma S, but not GMPPNP, inhibit rat skeletal mus...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00241a014
更新日期：1991-07-09 00:00:00
abstract：:TcmO is an O-methyltransferase that methylates the C-8 hydroxyl to Tcm B3, a four-ring aromatic intermediate in the tetracenomycin biosynthetic pathway of Streptomyces glaucescens. The gene encoding this enzyme was expressed in Streptomyces coelicolor CH999 together with the actinorhodin polyketide synthase (PKS) gene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952957y
更新日期：1996-05-28 00:00:00
abstract：:The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901184r
更新日期：2009-09-29 00:00:00
abstract：:The role of the 2'-hydroxyl group in RNA--protein interaction has been investigated using MS2 coat protein and its hairpin RNA operator as a model system. Derivatives of the MS2 translational operator were prepared where individual riboses were replaced by deoxyribose and their binding affinities to MS2 coat protein w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00038a033
更新日期：1995-09-26 00:00:00
abstract：:Recent studies have indicated that CYP3A4 exhibits non-Michaelis-Menten kinetics for numerous substrates. Both homo- and heterotropic activation have been reported, and kinetic models have suggested multiple substrates within the active site. We provide some of the first physicochemical data supporting the hypothesis ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051689t
更新日期：2005-11-01 00:00:00
abstract：:Transient contacts between denatured polypeptide chains are likely to play an important part in the initial stages of protein aggregation and fibrillation. To analyze the nature of such contacts, we have carried out a protein engineering study of the 102-residue protein U1A, which aggregates transiently in the wild-ty...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048509k
更新日期：2004-10-19 00:00:00
abstract：:The DNA binding domain of the yeast transcription factor Mbp1 is a winged helix-turn-helix structure, with an extended DNA binding site involving C-terminal "tail" residues. The thermodynamics of the interaction of the DNA binding domain with its target DNA sequence have been determined using fluorescence anisotropy a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702339q
更新日期：2008-06-17 00:00:00
