Nuclear Overhauser experiments at 500 MHz on the downfield proton spectra of 5S ribonucleic acid and its complex with ribosomal protein L25.

abstract：:The role of the C-domain sites of cardiac troponin C in the modulation of the calcium signal remains unclear. In this study, we investigated the effects of hypertrophic cardiomyopathy-linked mutations A8V, E134D, and D145E in cardiac troponin C on the properties of the C-domain sites. The A8V mutation had essentially ...

journal_title：Biochemistry

authors： Swindle N,Tikunova SB

更新日期：2010-06-15 00:00:00

abstract：:Endonuclease V from bacteriophage T4 is a well characterized enzyme that initiates the repair of ultraviolet light induced pyrimidine dimers. Scission of the phosphodiester backbone between the pyrimidines within a dimer, or 3' to an abasic (AP) site, occurs by a beta-elimination mechanism. In addition, high concentra...

journal_title：Biochemistry

authors： Latham KA,Lloyd RS

更新日期：1995-07-11 00:00:00

abstract：:Loop 181-197 of human thymidylate synthase (hTS) populates two conformational states. In the first state, Cys195, a residue crucial for catalytic activity, is in the active site (active conformer); in the other conformation, it is about 10 A away, outside the active site (inactive conformer). We have designed and expr...

journal_title：Biochemistry

authors： Gibson LM,Lovelace LL,Lebioda L

更新日期：2008-04-22 00:00:00

abstract：:Aspartoacylase catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate and is the only brain enzyme that has been shown to effectively metabolize NAA. Although the exact role of this enzymatic reaction has not yet been completely elucidated, the metabolism of NAA appears to be nec...

journal_title：Biochemistry

authors： Le Coq J,An HJ,Lebrilla C,Viola RE

更新日期：2006-05-09 00:00:00

abstract：:An oligodeoxynucleotide containing four thymines and covalently attached to an acridine derivative through its 3'-phosphate [(Tp)4(CH2)5Acr] was synthesized. Its conformation in solution was investigated by proton magnetic resonance. Both intramolecular interactions between the acridine dye and thymines and intermolec...

journal_title：Biochemistry

authors： Lancelot G,Asseline U,Thuong NT,Hélène C

更新日期：1985-05-07 00:00:00

abstract：:Twenty-five different temperature-sensitive point mutations at 20 sites in the lysozyme gene of bacteriophage T4 have been identified. All of the mutations alter amino acid side chains that have lower than average crystallographic thermal factors and reduced solvent accessibility in the folded protein. This suggests t...

journal_title：Biochemistry

authors： Alber T,Sun DP,Nye JA,Muchmore DC,Matthews BW

更新日期：1987-06-30 00:00:00

abstract：:Western blot analysis, using a polyclonal antibody to the 240-kDa endogenous inhibitor of the 20 S proteasome, revealed that the inhibitor is a component of the 26 S complex. Although isolated inhibitor displayed a single 40-kDa band on SDS-PAGE, the antibody detected a 55-kDa component in the 26 S proteasome complex....

journal_title：Biochemistry

authors： Li XS,Etlinger JD

更新日期：1992-12-08 00:00:00

abstract：:The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...

journal_title：Biochemistry

authors： Noguchi T,Inoue Y,Tang XS

更新日期：1997-12-02 00:00:00

abstract：:Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...

journal_title：Biochemistry

authors： Swart GW,Parmeggiani A

更新日期：1989-01-10 00:00:00

abstract：:A previously unknown polypeptide myotoxin, designated myotoxin a, was isolated for the first time from prairie rattlesnake (Crotalus viridis viridis) venom. Electrophoretic homogeneity of myotoxin a was shown in beta-alanine disc gel polyacrylamide gel electrophoresis and in isoelectric focusing gel electrophoresis. M...

journal_title：Biochemistry

authors： Cameron DL,Tu AT

更新日期：1977-05-31 00:00:00

abstract：:The gene for the human substance P receptor (NK-1) was cloned using cDNA probes made by the polymerase chain reaction from primers based on the rat sequence. The gene spans 45-60 kb and is contained in five exons, with introns interrupting at sites homologous to those in the NK-2 receptor gene. Analysis of restriction...

journal_title：Biochemistry

authors： Gerard NP,Garraway LA,Eddy RL Jr,Shows TB,Iijima H,Paquet JL,Gerard C

更新日期：1991-11-05 00:00:00

abstract：:Mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins R1 and R2, each inactive alone. The R1 protein binds the ribonucleotide substrates while the R2 protein contains a binuclear iron center and a tyrosyl free radical, essential for activity. The crystal structures of the corresponding Esc...

journal_title：Biochemistry

authors： Rova U,Goodtzova K,Ingemarson R,Behravan G,Gräslund A,Thelander L

更新日期：1995-04-04 00:00:00

abstract：:The coordination complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)3(3+)], which promotes RNA cleavage upon photoactivation, has been shown to target specifically guanine-uracil (G-U) mismatches in double-helical regions of folded RNAs. Photoactivated cleavage by Rh(DIP)3(3+) has been examined on a se...

journal_title：Biochemistry

authors： Chow CS,Barton JK

更新日期：1992-06-23 00:00:00

abstract：:The binding of the inhibitor 8-nitroxanthine to urate oxidase has been investigated by Raman and UV-visible absorption spectroscopy. The absorption maximum of 8-nitroxanthine shifts from 380 to 400 nm upon binding to the enzyme, demonstrating that the electronic structure of the ligand is perturbed. It has been propos...

journal_title：Biochemistry

authors： Doll C,Bell AF,Power N,Tonge PJ,Tipton PA

更新日期：2005-08-30 00:00:00

abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...

journal_title：Biochemistry

authors： Morgan TR,Shand JA,Clarke SM,Eaton-Rye JJ

更新日期：1998-10-13 00:00:00

abstract：:The mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involving primer 3' OH deprotonation, nucleophilic attack, and pyrophosphate leaving-group elimination, preceded by dNTP binding which induces a large-amplitude conformational change for Watson-Crick nascent base pairs. Ambi...

journal_title：Biochemistry

authors： Sucato CA,Upton TG,Kashemirov BA,Osuna J,Oertell K,Beard WA,Wilson SH,Florián J,Warshel A,McKenna CE,Goodman MF

更新日期：2008-01-22 00:00:00

abstract：:The cytokinin-active isoprenoid nucleosides of Agrobacterium tumefaciens transfer ribonucleic acid were identified by high-pressure liquid chromatography, permethylation, and mass spectroscopy. Besides the expected 6-[(3-methylbut-2-enyl)amino]-9-(beta-D-ribofuranosyl)purine (i6A) and its 2-methylthio derivative (ms2i...

journal_title：Biochemistry

authors： Morris RO,Regier DA,Olson RM Jr,Struxness LA,Armstrong DJ

更新日期：1981-10-13 00:00:00

abstract：:Electron nuclear double resonance (ENDOR) of protons at Type 2 and Type 1 cupric active sites correlates with the enzymatic pH dependence, the mutation of nearby conserved, nonligating residues, and electron transfer in heterologously expressed Rhodobacter sphaeroides nitrite reductase. Wild-type enzyme showed a pH 6 ...

journal_title：Biochemistry

authors： Zhao Y,Lukoyanov DA,Toropov YV,Wu K,Shapleigh JP,Scholes CP

更新日期：2002-06-11 00:00:00

abstract：:Many early investigations on triple helices have been devoted to the study of the triplex formed by dT*dA-dT base triplets in which the third strand is oriented parallel to the dA strand. We now describe an intramolecular triple helix with dT*dA-dT base triplets in which the pyrimidine third strand is oriented antipar...

journal_title：Biochemistry

authors： Dagneaux C,Liquier J,Taillandier E

更新日期：1995-11-14 00:00:00

abstract：:Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...

journal_title：Biochemistry

authors： Tian F,Lee KC,Hu W,Cross TA

更新日期：1996-09-17 00:00:00

abstract：:Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...

journal_title：Biochemistry

authors： Maly P,Wower J,Zobawa M,Brimacombe R

更新日期：1983-06-21 00:00:00

abstract：:Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...

journal_title：Biochemistry

authors： Slean MM,Reddy K,Wu B,Nichol Edamura K,Kekis M,Nelissen FH,Aspers RL,Tessari M,Schärer OD,Wijmenga SS,Pearson CE

更新日期：2013-02-05 00:00:00

abstract：:This work shows that the voltage across membranes in two very different preparations, lipid vesicles in suspension and individual HeLa cells under a microscope, is linearly related to the ratio of fluorescence excited from the two wings of the absorption spectrum of a voltage-sensitive dye. The dye di-4-ANEPPS [1-(3-s...

journal_title：Biochemistry

authors： Montana V,Farkas DL,Loew LM

更新日期：1989-05-30 00:00:00

abstract：:Pyridoxal 5'-triphospho-5'-adenosine (AP3-PL), the affinity labeling reagent specific for lysine residues in the nucleotide-binding site of several enzymes [Tagaya, M., & Fukui, T. (1986) Biochemistry 25, 2958-2964; Yagami, T., Tagaya, M., & Fukui, T. (1988) FEBS Lett. 229, 261-264], was used to identify the ATP-bindi...

journal_title：Biochemistry

authors： Hountondji C,Schmitter JM,Fukui T,Tagaya M,Blanquet S

更新日期：1990-12-25 00:00:00

abstract：:Rad51 protein, a eukaryotic homologue of RecA protein, forms a filamentous complex with DNA and catalyzes homologous recombination. We have analyzed the structure of Xenopus Rad51 protein (XRad51.1) in solution by small-angle neutron scattering (SANS). The measurements showed that XRad51.1 forms a helical filament ind...

journal_title：Biochemistry

authors： Ellouze C,Kim HK,Maeshima K,Tuite E,Morimatsu K,Horii T,Mortensen K,Nordén B,Takahashi M

更新日期：1997-11-04 00:00:00

abstract：:We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...

journal_title：Biochemistry

authors： Bucci E,Fronticelli C,Gryczynski Z

更新日期：1991-04-02 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:The complex kinetic behavior commonly observed in protein folding studies suggests that a heterogeneous population of molecules exists in solution and that a number of discrete steps are involved in the conversion of unfolded molecules to the fully native form. A central issue in protein folding is whether any of thes...

journal_title：Biochemistry

authors： Walkenhorst WF,Green SM,Roder H

更新日期：1997-05-13 00:00:00

abstract：:We report the 1H and 15N chemical shift assignments along with an NMR-derived preliminary structure for DnaJ(1-78), a highly conserved N-terminal domain of DnaJ, the Escherichia coli Hsp40 homolog. This 9 kDa domain is believed to cooperate with DnaK, the E. coli Hsp70 homolog, in regulating a variety of cellular func...

journal_title：Biochemistry

authors： Hill RB,Flanagan JM,Prestegard JH

更新日期：1995-04-25 00:00:00

abstract：:The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...

journal_title：Biochemistry

authors： Kelly SJ,Butler LG

更新日期：1977-03-22 00:00:00